... a snake.were / wouldn't want 14 My brother managed to kill the snake just at the time when I (be) almost exhausted If he (be) a little late, I (kill) by the snake.was / had been / would have ... a snake 14 My brother managed to kill the snake just at the time when I (be) had been almost exhausted If he (be) had been a little late, I (kill) would have been killed by the snake 15 Had I ... in a snake 14 My brother managed to kill the snake just at the time when I were almost exhausted If he had beena little late, I would have killed by the snake 15 Had I know you were ill, I (visit)would...
Ngày tải lên: 23/01/2014, 07:20
Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx
... Furthermore, the pro-peptide of GDF-8 impairs interaction of the mature part with its receptors [16] In the case of BMP-9, the pro-peptide appears not to alter significantly the activity of the ... interested in the role of the 263 amino acid pro-peptide of BMP-2, because evidence accumulated over recent years has shown that the pro-forms of growth factors can modulate the activities of the mature ... patients [39], and a role of the receptor in the development of the arthritis has been discussed [26] The data presented here provide clear evidence that the pro -form of BMP-2 can interact with...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
... in the main chain of the subunit, or in the interaction of subunits within the trimer that linked the conformation of residues 120–125 to the position of the active site lip and closure of the ... the dTTP complex to accommodate the 5-methyl group of the thymine moiety As a result, the Ala124 carbonyl was moved from the 4-oxo ⁄ 4-amino group of the bound nucleotide and the side chain of ... observation is that due to the proximity of residues 120–125 from each subunit in the centre of the trimer, the side chains of Val122 of one subunit and Thr123 of the neighbouring subunit are...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu The 2012 Nexus Event – An Unknown Form Of Energy Is Coming Our Way pdf
... to the ancient people Besides that, we hear about the hidden Astrology and the various aspects of the 12 signs of Zodiac, than about the effect which comes from the alignment of the Pole of our ... equator and the alignment of Earth with the center of the Milky Way This Cosmic Cross is considered as embodiment of the Sacred Tree, (The Tree of Life), a tree which is remembered in most of the ancient ... 28 March 2013 when in fact is the end of the Sun-Venus calendar and in the same time the start of Sixth Sun During this process from the direction of the center of the Milky Way a cosmic rays will...
Ngày tải lên: 19/02/2014, 03:20
Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
... Although the SH3 domain of Noxo1 participates in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown In the process of cloning of human Noxo1, some spliced transcripts of the ... presentation of domain structures of Noxo1 and the location of the five-amino-acid insertion in the PX domain SH3, Src-homology domain; PRR, proline-rich region (C) Sequence alignments of the PX domains of ... To compare the amounts of the two variants expressed in the testis, we performed the PCR using the primers ‘d’ and ‘c’, where the cDNAs for both Noxo1b and Noxo1c are amplified as the insert region...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot
... In the first step of the enzymatic reaction, a nucleophilic attack is performed on the anomeric carbon of the sugar substrate by the carboxylate of the Asp54 acting as the primary nucleophile, forming ... anomeric carbon of fructose The leaving group is carboxylate of Asp54 Dimerization The asymmetric units of the crystals of both the apo and complexed form of the enzyme consist of dimers with ... results of MS of recombinant b-fructofuranosidase are close to the theoretical value of the molecular mass A peak of molecular ion at m ⁄ z = 58 879 Da was observed on the MALDITOF mass spectrum The...
Ngày tải lên: 06/03/2014, 00:21
Proposal for a COUNCIL DECISION authorising enhanced cooperation in the area of financial transaction tax potx
... (pre-)financed by the European taxpayer in the course of 2008 to 2012 These operations, together with the faltering of economic activity caused by the spread of uncertainty about the stability of the overall ... cooperation in the area of financial transaction tax THE COUNCIL OF THE EUROPEAN UNION, Having regard to the Treaty on the Functioning of the European Union, and in particular Article 329(1) thereof, Having ... fully, at the scale of all 27 Member States10 Risks of fragmentation of the internal market and of a distortion of competition will first of all be reduced and/or avoided within the scope of the FTT...
Ngày tải lên: 06/03/2014, 19:20
Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... coprecipitated only the slowly migrating form of RNA pol II (Fig 1) This suggested that Pol e associates with the RNA pol IIO isoform To characterize further the phosphorylation status of the RNA pol ... isoform IIO that exhibits reduced electrophoretic mobility compared with the IIA isoform Whereas the polyclonal antiRNA pol II antibody precipitated both the fast and slowly migrating forms of ... Following phosphatase treatment, the cellular fraction of RNA pol II that associated with Pol e showed increased mobility, corresponding to that of isoform IIA The isoform IIO was also lost from RNA...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of the secondary structure of ... for further study The concentration of the proteins was determined by the method of Lowry et al [14] and the purity was examined by SDS/PAGE [15] Assay of human PKIb activity The activity of purified ... Characterization of PKI gamma, a novel isoform of the protein kinase inhibitor of cAMPdependent protein kinase J Biol Chem 272, 18169–18178 Gamm, D.M & Uhler, M.D (1995) Isoform-specific differences in the...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot
... that of its unglycosylated form, though the DV and DGP values were almost the same The Pm value of proCPY was higher than that of its unglycosylated form This is due to the higher DV value of the ... that the precursor form was less thermally stable than the mature form, regardless of the carbohydrate moiety After the temperature was lowered from the highest temperature tested to 25 °C, the ... (Fig 1A), indicating that the thermal unfolding process of the precursor form follows a two-state transition The ratio of the unfolding enthalpy (DHcal) to the van’t Hoff enthalpy (DHv) was 1.05...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
... between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface of the protein because of the interactions of the cofactor with the protein ... are comparable, the Tg values of the proteins vary slightly; there is a difference of almost C between the Tg values of the proteins, the value being higher for the holo form There have been ... unfolding thermodynamic parameters of the two forms are given in Tables 13 In the entire experimental regime, it is seen that the holo form presents better stability than the other form (Fig 4C) The...
Ngày tải lên: 16/03/2014, 10:20
The Evolving Spatial Form of Cities in a Globalising World Economy pot
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: The pharmacological chaperone isofagomine increases the activity of the Gaucher disease L444P mutant form of b-glucosidase docx
... long half-life of the enzyme The inability to detect IFG in the brain of L444P GCase mice is most probably a result of the dose of IFG tartrate administered (20 mgÆkg)1Æday)1) and the relatively ... compilation ª 2010 FEBS R Khanna et al ages further evaluation of this molecule for the treatment of neuronopathic forms of Gaucher disease The administration of IFG to L444P GCase mice for up to 24 ... stress, which, in turn, could affect the organ weights and plasma marker levels of these animals Whether the effects of IFG on the organ weights and plasma markers of inflammation in L444P GCase mice...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... allowing them to re-associate as domain-swapped dimers Apart from differences in the course of the protein backbone at the point of transition, in the swapped dimer the overall structure of the functional ... with the nucleobases pointing towards the protein surface The 5¢-to-3¢ polarity of the ligand follows that of most other nucleic acid complexes of OB-fold proteins, starting in the vicinity of ... shields the hydrophobic nucleobases and the hydrophobic-binding platform of the protein below them from the polar solvent (Fig 2B) The sugar of T1 from chain C maintains a C4¢-exo pucker, whereas the...
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4 doc
... structure of the soluble form of CLIC4 the backbone and side chain of Asn34 and the side chain of Lys24 This crystal contact stabilizes the structure of the leading side of the foot loop The putative ... the side chain of Asn81 The intervening residues of the C-terminal extension interact with the neighboring monomer via hydrophobic contacts Comparison with the structure of the soluble form of ... reduction in the size of the protein, leaving a kDa fragment [4] This supports the hypothesis that the integral membrane form of the protein has a single transmembrane region near the N-terminus...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo Y học: The presence of phosphate at a catalytic site suppresses the formation of the MgADP-inhibited form of F1-ATPase potx
... observed in the presence of Pi [18] Here we examined the effects of Pi on the formation of the MgADPinhibited form of the a(W463F)3b(Y341W)3c subcomplex The extent of ADP inhibition of the a(W463F)3b(Y341W)3c ... addition of a3b3c to 1.2 mL of the assay mixture One unit (U) of activity is de®ned as the activity of lmol ATP hydrolysedámin)1 The ATPase activities at the initial phase (the slope of A340 between ... concentrations of MgADP plus various concentrations of potassium phosphate was measured Increasing concentrations of Pi prevented the formation of the MgADP-inhibited form of the a(W463F)3b(Y341W)3c...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo khoa học: "TWO THEORIES FOR COMPUTING THE LOGICAL FORM OF MASS EXPRESSIONS" doc
... (Note also the comprehensive denotation of edible) No other theory we know of can account for the validity of these two arguments Both of these theories take it that is wine is true of the (abstract) ... assigning the same logical form to both theories Nonetheless, the meaning postulates of the two theories will differ for these.) In addition to the sorts of examples stated above, both these theories ... true of unions of elements of the semilattice) These predicates are not only true of the conventional kinds but also of conventional servings such as the bottle of wine on the table or the 250ml...
Ngày tải lên: 24/03/2014, 01:21
A Study on the Effects of Argentine Tango as a Form of Partnered Dance for those with Parkinson Disease and the Healthy Elderly pptx
... ongoing basis The touch of others, the rhythm of the music, and the novelty of the experience all contributed to the beneficial effects The sense of community spirit was evident when the tango group ... source of social support for the members The pace of the Argentine tango lessons was at the level of the average person with PD The control tango group may have experienced greater gains had their ... requested that they have lunch together at the end of the sessions During the dance classes, they were very helpful to one another Although spouses did not always dance together, all the control...
Ngày tải lên: 28/03/2014, 20:20
BUREAU OF THE PUBLIC DEBT: Legal Aspects of United States Savings Bonds pot
... on the Series H/HH bonds, unless, under the grantor trust provisions of the Internal Revenue Code, the owner of the bonds is treated as the owner of the portion of the trust represented by the ... interest on the reissued bonds If the bondowner is treated as the owner of that portion, the accumulated interest continues to be income of the bondowner rather than of the trust and, therefore, the ... in the name of one individual only (or to which the decedent was the sole person entitled at the time of death) becomes a part of the estate on the death of the owner (See Section 315.70(a) of...
Ngày tải lên: 29/03/2014, 03:21