... of
PEX11(andTIM)inthecell.
The study ofthe importance of TbPEX14 for the parasite
has shown that the suppression of peroxin expression by
RNAileadstoareductioninthegrowthrateofT. brucei,
both ofthe bloodstream ... brucei because the level of PEX14
mRNA in these cells was significantly lower (not shown).
In order to determine the influence ofthe reduction of the
expression of TbPEX14 on theimportof glycosomal ... analysing the subcellular distribu-
tion ofthe glycosomal enzymes GAPDH, ALD and TIM
upon reduction ofthe PEX14 mRNA level. RNAi
treatment of T. brucei resulted in the release of part of the
glycosomal...
... for the nuclear import. Results on mt9
suggested that phosphorylation ofthe tyrosine residue
is not related to the nuclear import. Then, the role
of these two residues PY in nuclear importof ... 2005 FEBS 3985
of D02 was 4.0 nm and the K
D
of DNS 9.2 nm, that is
double that of D02. This larger K
D
was accounted for
by the twofold larger k
d
of DNS than that of D02 and
their nearly similar ... sequences of D01 and D02 were
found as an NLS [29,30]. However, there is no clear
evidence for the nucleocytoplasmic shuttling activity
of NLS and the involvement of Trn-1 in the nuclear
import. The...