... II and Nco I The amplified fragment was cloned into pd19 by digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI The fragment was ligated into the plasmid and the ... allowing the red blood cells to settle for 30 The supernatant was harvested and centrifuged for at 500 g The cells were then resuspended in 0.2 Â NaCl/Pi to induce red blood cell lysis for and then ... blocked for h with DMEM containing 1% BSA Blood cells and cell lines were labeled with calcein-AM (5 mL per 108 cells) for 30 at 41 8C Cells were then washed twice in HBSS and added to the blocked...
Ngày tải lên: 24/03/2014, 04:21
... of m and the length of the second log varied from to m depending on the soundness of the upper stem The average number of annual rings in the lower disk was 149 and 131 in the upper one The boundary ... applied to test whether the sapwood and the outer and the inner heartwood differed from each other in the studied wood characteristics The pair-wise comparisons between the stem sections were ... calculate the overall means and standard deviations (sd) for the sapwood and the outer and the inner heartwood The coefficients of variation (CV%) were used to describe the variation among the trees...
Ngày tải lên: 08/08/2014, 01:21
Báo cáo khoa học: "Foliation of spruce in the Giant Mts. and its coherence with growth and climate over the last 100 years" pdf
... chronologies 158 C Sander and D Eckstein CORRELATION AND RESPONSE FUNCTION ANALYSIS The time series of the different foliation and growth variables were compared with each other by correlation ... Variation of foliation and growth variables Boxes represent values between the lower and the upper quartile including the median, while the whiskers show the range (min–max) Dots (°) and asterisks (*) ... more and more by proventive shoots, competition for water and nutrients lowers the supply of regularly formed shoots and therefore results in a lower number of needle primordia On the other hand,...
Ngày tải lên: 08/08/2014, 14:21
Báo cáo y học: "Tumor microenvironments, the immune system and cancer survival" ppt
... may lead to elimination of the cancerous cells (b) If elimination is unsuccessful, the immune system and the cancer can reach an equilibrium in which immune cells keep the cancer in check but cannot ... transformation of cells in a normal layer (diamond-shaped cells) into cancerous cells (with irregular shapes), attack by various different cell types of the immune system (indicated by round cells) may ... expressed in the non-malignant cells Therefore, in this study, patient outcome was most directly associated with the type of immune response, not the expression profile of the cancer cells themselves...
Ngày tải lên: 14/08/2014, 14:21
thoughts on the funding system, and its effects
... by the hand of industry, its waters become at once the ornament of the country and the instrument of its commerce The deity of the stream, then weary of beholding only mallards, and pollards, and ... only shews the direct and immediate loss which proceeded from the adoption of the funding system; the differences between the price paid to the state by the contractors for stock, and the price ... be without hope and without remedy Yet the woods and the marshes, the bogs and the glens, among which the few miserable natives of Britain sought a scanty and precarious subsistence, when the...
Ngày tải lên: 05/11/2014, 13:25
The herpetofauna of the peruvian dry forest along the andean valley of the marañón river and its tributaries, with a focus on endemic iguanians, geckos and tegus
... through the deep valley between the Cordillera Occidental and the Cordillera Central At its confluence the Marañón River merges with the Huallaga River and other smaller rivers to form the Amazona ... areas The dry forest ecoregion of the Marañón River and its tributaries is located in the Central Andes confined by the Cordillera Occidental to the west, and by the Cordillera Central to the East ... junction with the Rio Chinchipe Located near the Rio Marañon, to the West of the river Located near the Rio Marañon, to the West of the river Located near the Rio Marañon, to the West of the river...
Ngày tải lên: 19/11/2015, 15:51
Tài liệu MECHANICAL PROPERTIES OF THE HEART AND ITS INTERACTION WITH THE VASCULAR SYSTEM ppt
... into the pulmonary vascular system which contains the right ventricle, the pulmonary arteries, the pulmonary capillaries and pulmonary veins and the systemic vascular system which contains the ... left ventricle, the systemic arteries, the systemic capillaries and the systemic veins Each pump provides blood with energy to circulate through its respective vascular network While these pumps ... intraventricular pressure, the functioning of the cardiac valves, and something about the load against which the ventricles contract (i.e., the properties of the systemic and pulmonic vascular systems) On a...
Ngày tải lên: 22/02/2014, 09:20
Tài liệu The NULL Encryption Algorithm and Its Use With IPsec ppt
... NULL and IPsec November 1998 [ESP] specifies the use of an optional encryption algorithm to provide confidentiality and the use of an optional authentication algorithm to provide authentication and ... computing authentication data which covers the data portion of a packet as well as the immutable in transit portions of the IP header ESP_NULL does not include the IP header in calculating the authentication ... ESP can then be used to provide authentication and integrity without confidentiality Unlike AH these services are not applied to any Glenn & Kent Standards Track [Page 3] RFC 2410 NULL and IPsec...
Ngày tải lên: 14/02/2014, 16:20
Tài liệu Báo cáo khoa học: From heart to mind The urotensin II system and its evolving neurophysiological role ppt
... particularly with regard to arousal and sleep Molecular constituents of the UII system Presently, three molecules are known to form the UII system: the UII receptor and the peptides UII and urotensin-related ... receptor or its ligands will provide a rational approach for the investigation of the neurophysiological role of the UII system While our understanding of the molecular constituents of the UII system ... tested and that will aid the understanding of UII’s interaction with other sleep and wake promoting systems Conclusions The discovery of UII as a direct activator of central cholinergic neurons and...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot
... internal b-sheet of the b-sandwich domain The last four amino acids of the native sequence at the C-terminus and the His-tag protrude from the b-sandwich domain and interact with the Nterminal b-propeller ... resembles the shape of a fishtrap The active site is located at the bottom of the axial funnel created by the interblade loops and the loops between b-strand 2–3 of each blade The second funnel on the ... located in the funnel created by the five blades of the b-propeller on its axis (Fig 2A) The first strand of each blade creates an internal surface of the ‘whirl’ and all five strands go from the substrate...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo "On the asymptotic behavior of delay differential equations and its relationship with C0 - semigoup " potx
... satisfies the hypothesis of theorem (2.3) then the solution of Eq.(12) in the case µ = is asymptotic equivalence to the solution of Eq.(12) in the case µ = Ackowledgments This paper is based on the ... we can show that the null solution x(t) ≡ of Eq.(4) is uniformly stable ii) By assumption (ii) of the theorem there exist the positive constants C and λ δ= Ce−λt , T (t) ∀t > By the similar argument ... [12]), we can get the following result: Theorem 2.1 Suppose (T (t))t≥0 is C0 - semigroup with the generator (A, D(A)) The following assertions are true: (1) If T (t) M, ∀t ≥ 0, then the null solution...
Ngày tải lên: 22/03/2014, 11:20
Báo cáo khoa học: "METONYMY: REASSESSMENT, SURVEY OF ACCEPTABILITY, AND ITS TREATMENT IN A MACHINE TRANSLATION SYSTEM S" ppt
... acceptance in the group to which the expression belongs to in the result of the survey, the system should accept it for analysis, and come up with some metonymic reading using its inference mechanism ... ideas using existing systems such as the ULTRA MT system (Wilks and Farwell 1990) and the Metallel metonymic analysis program (Iverson and Helmreich 1992) Iverson, Eric and Helmreich, Stephen ... allow a metonymic expression which corresponds to the original form, then the system should use the result of the metonymic inference and come up with an acceptable translation REFERENCES Fass,...
Ngày tải lên: 23/03/2014, 20:20
scientific american - 1993 09 - special issue - life, death, and the immune system
... interlopers and guide the bodyÕs defenses This process is crucial to the function of the immune system and its failure 80 How the Immune System Recognizes the Body Philippa Marrack and John W Kappler The ... American, Inc Life, Death and the Immune System By defining and defending the self, the immune system makes life possible; malfunction causes illness and death Study of the system provides a unifying ... Þrst is the innate immune system innate in the sense that the body is born with the ability to recognize certain microbes immediately and to destroy them The second is the adaptive immune system, ...
Ngày tải lên: 12/05/2014, 14:50
báo cáo hóa học: " Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers" pptx
... preconditioned with methanol and water After lavage with mL water and mL hexane, the analyte was eluted with 3.5 mL dichloromethane The extract was concentrated to about 200 μL and injected into a HPLC system ... performed the PAH analysis and contributed to the writing of the manuscript MG/MJR: participated in the study design and contributed to the scientific content of the manuscript and its revision ... Christine Kohler and Dr Nancy Hopf (Institute for Work and Health) for their help in the metal/creatinine analysis, and comments on the manuscript The medical team of the Institute for Work and Health...
Ngày tải lên: 20/06/2014, 00:20
báo cáo hóa học:" The impact of iron overload and its treatment on quality of life: results from a literature review" pptx
... endure the daily task of inserting a needle into their child and constant battles with their child in order to comply with the treatment regimen, which would then increase parental stress-levels These ... stated that their sex lives and relationships were affected by their treatment (4/ [44%]), and some stated that the treatment affected their self-esteem (2/9 [22%]), with some stating that they chose ... from the youngest age through adolescence and therefore may have a negative impact on the parents' relationship with their child and may also cause the child to become over-dependent on their...
Ngày tải lên: 20/06/2014, 16:20
The Modulation of Optical Property and its Correlation with Microstructures of ZnO Nanowires ppt
... electrons in the valence band (VB) can be excited to the conduction band (CB) to form the photo-generated electrons in the CB and the same amount of holes in the VB The holes in the VB are prone ... solution and 60 mg ZnO nanowires The UV lamp (6 W) was placed in the center of the tube and surrounded by the reactor Prior to irradiation, the solution was sonicated for 30 and then stirred in the ... which is quite different from the nanowires grown in the air atmosphere by comparing with Fig 2a, b On the other hand, the oxygen content can also affects the shape of the nanowires In our experiments,...
Ngày tải lên: 22/06/2014, 00:20
Báo cáo sinh học: "Parasite immunomodulation and polymorphisms of the immune system" ppsx
... [8] and animal models [9] The best-understood of these suppressive systems is the regulatory T cell (Treg), a cellular safety catch on the immune response that normally blocks autoimmunity and ... exceptionally susceptible to either infection or pathology, and a deeper understanding of the intimate co-evolution of pathogens and the immune system 10 11 12 13 14 Acknowledgements The author’s research ... proteins and display them for recognition by T cells; TCR, T cell receptor, the highly variable receptor through which T cells recognize their targets This may be the explanation for the link...
Ngày tải lên: 06/08/2014, 19:20
Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... inducing the local induction of Th3 or Tr1 cells [74], extends this paradigm The fate of the TR cells induced at the site is not known — whether these cells exist only transiently then die, whether they ... setting T cells will become activated, and will recruit other T cells and other inflammatory cells and mediators to the site As the response becomes mature, a group of regulatory T cells will ... they persist in the body as TR cells, or whether they return to their previous role as nonregulatory resting T cells remains unknown The balancing act required by the immune system to attack foreign...
Ngày tải lên: 09/08/2014, 01:24
báo cáo khoa học: "Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia" doc
... conception and design laboratory study XH, SHC, QS, LJY, and BL performed the laboratory technique process and the laboratory analyses LYZ, SXG and XD were responsible of the patient’s treatment and ... 0.995) The amplification efficiencies of BCL2L1 and the b2M control gene were 95.30% and 95.16%, respectively, and the melting curves are shown in Figure 2C PCR products from the b2M control gene and ... clinical data YQL and XH coordinated the study and helped to draft the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing...
Ngày tải lên: 10/08/2014, 22:21
Báo cáo y học: " The development of a knowledge test of depression and its treatment for patients suffering from non-psychotic depression: a psychometric assessment" ppt
... of the following statements about psychotherapy is FALSE? a Both individual and group talk therapy provides an opportunity to express and discuss thoughts and feelings with the therapist b Therapy ... in the three groups with respect to age distribution, durations of illness, and the duration of the current episode, and the number of visits with a psychiatrist over the last six months There ... could handle the episode themselves On the other hand, those who sought help felt that their experience of the episode was too painful, lasted too long, and disrupted their interpersonal and role...
Ngày tải lên: 11/08/2014, 17:20