the human major histocompatibility complex and dna based typing of human leukocyte antigens for transplantation
Báo cáo khoa hoc:"The porcine Major Histocompatibility Complex and related paralogous regions: a review" ppsx
Ngày tải lên :
09/08/2014, 18:21
... glycoproteins These two classes of glycoproteins differ in their structure, the cells and tissues in which they are expressed, the origin of the peptides they present to the T cells and the subsets of T ... among other genes harbours the HLA complex, and the small arm of the swine chromosome and part of its pericentric region [16] One may therefore expect, that the genes located near the HLA complex, ... to the SLA Ia genes, and like the human and mouse Ib genes, have no counterparts in either of these species The functions of the swine Ib genes are not known, but their tasks may include the...
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Báo cáo y học: "Critical role of the major histocompatibility complex and IL-10 in matrilin-1-induced relapsing polychondritis in mice" doc
Ngày tải lên :
09/08/2014, 01:24
... not proceed in relapses as they did in the QD and B10.Q mice, and therefore resulted in a lower mean score than for the other strains (Fig 1c) Table Susceptibility of mouse strains to immunization ... phase at score or at the end of the experiment at day 130 The tissue was immediately either snap-frozen at -70°C or fixed in 4% paraformaldehyde solution for 24 hours and further embedded in paraffin ... correlation between the titer of anti-matrilin-1 antibodies and clinical symptoms, in both the IL-10-deficient and the heterozygous mice Surprisingly, several of the IL-10 knockout mice, all of which were...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
Ngày tải lên :
18/06/2014, 16:20
... day 14 and day 28 by counting the number of foot faults in the left and right forelimbs The sum of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, ... forelimbs, whereas the differentiation of forelimb foot faults (DOFFF) is the difference between the number of faults for the right and left forelimbs SOFFF and DOFFF were used as an index of motor deficit ... of 95°C for 15 s, 60°C for min, and finally 95°C for 15 s, 60°C to 95°C for 30 s, and 95°C for 15 s Quantification of the gene of interest was accomplished by measuring the threshold cycles and...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the enzyme for the other substrate (GST-IjBa) ... not block the activity of another IKK isoform, IKK1 Consequently, IjBa may still be phosphorylated by the IKK1 isoform when the IKK1 isoform is present and activated in the system Another could ... solution and 100 lL of wash solution dispensed into the wells The plates were inverted to remove the wash solution for the last time and replaced with 200 lL of wash buffer The plates were sealed and...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 2
Ngày tải lên :
14/09/2015, 14:07
... minimum quantity of DNA needed for SNP genotyping was 80µl of DNA at a concentration of not less than 50ng/µl (minimum of 4mg of DNA per sample) DNA quantification was mostly performed by DNA staining ... 1.5mM MgCl2, 1.5µl of 5µM of the forward primer, 1.5µl of 5µM of the reverse primer, 1.5µl of 100% DMSO (Sigma-Aldrich, USA), 100ng of genomic DNA and 0.2µl of High Fidelity Taq DNA polymerase (Roche ... reactions for HLA-A,-B and -C were performed in total reaction volumes of 50µl comprising of: 5µl of 2mM dNTPs, 5µl of 10xPCR buffer, 5µl of 5µM 5´ primer, 5µl of 5µM 3´ primer, 100ng of genomic DNA and...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 3
Ngày tải lên :
14/09/2015, 14:07
... Disequilibrium and Haplotype Map of the Chromosome 6p and the Major Histocompatibility Complex To characterize the genetic variation and patterns of linkage disequilibrium (LD) of the human chromosome 6p and ... analyse the LD structure of the chromosome 6p and the MHC A high resolution SNP map was then constructed to analyse the LD of the MHC in greater detail The corresponding sections in the results ... several islands of SNPs in high pairwise LD standing out in contrast with the relatively uniform level of equilibrium in the rest of the chromosome arm At an average marker density of SNP every...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 4
Ngày tải lên :
14/09/2015, 14:07
... 0.3) by any of the 1877 SNPs Panel B: Of these poorly represented SNPs, the majority of them are present at a frequency of less than 5% in the population, and thus not informative in the Han Chinese ... composition of ethnic groups One of the stated goals of the human HapMap project was to develop a set of tag SNPs that could guide the selection of SNPs and reduce genotyping redundancy for genome ... in the rate of decay of LD across the different sub-regions of the MHC The class I, class III and extended class II segments show a level of LD similar to the MHC average, but SNP pairs in the...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 5
Ngày tải lên :
14/09/2015, 14:07
... segments of clustered EHH decay are consistent with the location of established recombination hotspots, the locations of the latter are mapped onto the EHH plots There are sources of information for ... extent of EHH; the half-life of EHH is about 1Mb for alleles A*0207, B*4601 and B*1502, and for the other CEH associated alleles there are no significant differences from non-CEH alleles The segments ... onto these sequences, the same 249kb SNP block was derived for these cell lines The two tag SNPs also capture the DR haplotypes in these cell lines, confirming the validity of the tags and indicating...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 6
Ngày tải lên :
14/09/2015, 14:07
... 2005), and using these as the basic unit for comparisons will succinctly describe similarities and differences between CEHs For the local Chinese CEHs, the reference haplotypes for the A2B46-DR9 and ... position 29,594,500 Based on of 10 chromosomes, the conservation of the A33-B58-DR3 haplotype stretches from RFP at the 29Mb mark to 33Mb at the very least, a full 4Mb of the MHC In the chromosome ... segment of strong LD is seen telomeric to the MHC and is therefore likely that the A33-B58-DR3 CEH is conserved way past the telomeric boundary of this SNP map 131 132 The colours indicate the SNP...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 7
Ngày tải lên :
14/09/2015, 14:07
... plot across the segment The position of the hotspot segment mapped by Cullen and co-workers is indicated by the red bar at the top of the chart EHH values of HLA haplotypes decay of across this ... haplotypes of each individual shown together with the linked HLA-B allele Half of the samples are homozygous leading to the telomeric edge of the 4.5kb interval with the other half homozygous to the ... Location of the Hotspot Telomeric to HLA-F This figure shows the location of the hotspot mapped to a region telomeric to the HLA-F locus The genotypes of the 19 re-sequenced samples used to define the...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 8
Ngày tải lên :
14/09/2015, 14:07
... to identifying the genetic aetiology of these diseases lies in the understanding of the LD structure of the MHC LD describes the non-independence of alleles at different loci, and knowing how ... cholera pandemic, resulting in the rapid increase in the frequencies of the protective allele The DNA in the segments surrounding the protective allele are swept along with the allele, and if the ... highlighting the ancestral proximity of the populations, even though the former consists mainly of southern Chinese while the latter is more skewed towards the northern Chinese The tight correlation...
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A linkage disequilibrium map of the human major histocompatibility complex in singapore chinese conserved extended haplotypes and ancestral blocks 1
Ngày tải lên :
14/09/2015, 14:07
... Introduction 1.1 The Human Major Histocompatibility Complex (MHC) 1.1.1 Features of the Human MHC The human Major Histocompatibility Complex (MHC) is a gene dense segment on the short arm of the human chromosome ... such as the large regions of complex polymorphism in the MHC, the RCCX module and the HLADRB locus The RCCX module is a segment of DNA that includes part of the STK19 (RP) gene, C4A/B, CYP21 and ... (Svensson and Andersson 1997) 1.1.2 MHC and Disease Given that the MHC is at the heart of the human immune system, it is not surprising that the MHC is associated with the majority of autoimmune and...
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báo cáo khoa học: "Linkage in cattle between the major histocompatibility complex (BoLA) and the M blood group system" doc
Ngày tải lên :
09/08/2014, 22:23
... family of the bull VALMIN Table gives the lod-score values obtained for M’ and for the M, factor, and shows clearly the existence of genetic linkage between the BoLA complex and each of » the factors ... matings, and the segregation of the gametes within the offspring The genes for M’ and M, was inherited respectively from sires and from sires and cow Only putative recombinants occured within the ... France), and from the university herds in the U.S.A at The Ohio State University (Department of Dairy Science) and at the University of Wisconsin (Department of Agriculture) They consisted of dam-offspring...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
Ngày tải lên :
12/08/2014, 01:22
... Digene®) The intensity of the light was proportional to the amount of target DNA in the sample Estimation of HPV viral load Ratio of relative light unit (RLU) of the sample/mean of RLU of three ... (HC-II) and approved by the US food and drug administration (FDA) The performance characteristics of HC-II assay and the PCR for detection of HPV DNA have been compared in cervical lesions and results ... rate of HPV 16 E6 DNA positivity was nearly 26% by PCR and 27.4% by the HC-II test in case of OSMF While in case of OSCC the HPV DNA positivity were 32.4% by the PCR and 31.5% by the HC-II test Therefore,...
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Báo cáo khoa học: "Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows" pps
Ngày tải lên :
07/08/2014, 17:22
... Gorham JR The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use ... isoforms would have lower rates of positive selection of CD4+ helper T cells in their thymuses However, the number of class II isoforms also influences negative selection Models of positive and ... from the normal, mastitisresistant population reflects both the effects of infection and genetic susceptibility The sum of percentages of MGS mononuclear leukocytes stained by antibodies for the...
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Báo cáo sinh học: "Divergent selection for humoral immune responsiveness in chickens: distribution and effects of major histocompatibility complex types" ppt
Ngày tải lên :
14/08/2014, 19:22
... combination of haplotypes Serological typing was performed on the parents of the 8th generation, on all the females and the selected males of the 8th generation, and on all the birds of the 9th and 10th ... L), of the the fixed effect of the lth MHC genotype within the kth line, the random additive genetic effect on the Ab titer in the mth chick and eijklm = a random error The fixed effect of generation ... using the following mixed model: Where: = the Ab titer of the mth chick, p = a i generation = the fixed effect of the ith generation j sex = the fixed effect of the = the fixed effect of the kth...
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Báo cáo sinh học: "Effects of major histocompatibility complex on antibody response in F 1 and F 2 crosses of chicken lines" ppt
Ngày tải lên :
14/08/2014, 19:22
... = = the Ab titer of the kth chick, a constant, the fixed effect of the ith sex of the chick, the fixed effect of the jth MHC genotype, the random additive genetic effect on the Ab titer in the ... and Geno being the rj estimated effects of MHC genotypes of haplotypes i and j, and r and j, respectively, and p being the number of pairwise combinations Methods II and III were applied in the ... agreement with those of obtained from the selected lines than from the C line The relative importance of the MHC genotype and the remaining genotype on the variation of the Ab titer in the F were calculated...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
Ngày tải lên :
20/02/2014, 02:21
... s, and indicate re-oxidation of the flavin and the formation of the hydride-Meisenheimer complex of TNT (C) As for panel B except the spectral changes are recorded over an extended time period and ... substrates: in the first, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive conformation Rescue of the unreactive ... relevant 2-electron reduced form of the enzyme-steroid complex We conjectured that the reduced form of the enzyme might direct binding of the steroid in the flipped conformation, thus facilitating...
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The Breast Cancer Epidemic: Modeling and Forecasts Based on Abortion and Other Risk Factors potx
Ngày tải lên :
06/03/2014, 02:21
... occupation The Scottish ratios of mortality to incidence for the social classes were used to derive an approximate gradient of incidence for England The modeling for England for the age groups 45–49 and ... range 45–49, and the latest abortion and fertility rates Fall 2007 75 entered, the 2006 TAR for Northern Ireland is 0.16, the latest TFR is 1.87, and the forecast increase in the rate of cancer ... smoothing the graph of cohort cancer incidence for Northern Ireland it was possible to fit the model and make estimates With this model fitted on the available years of data to 2004 for the age...
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Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx
Ngày tải lên :
16/03/2014, 18:20
... characteristic of the closed conformation It further strengthens the contact of the Arg side chain with bL, already formed in the binary complex with 3-PG The formation of a new H bond in the closed ... T, and IN and IU are the intercepts and mN and mU are the slopes of the pre- and post-transitional base lines of the raw data, respectively Quantitative analysis of the experimental data Thermal ... origin of the increased stability of the closed conformation In the structures of the two closed ternary complexes of Thermotoga maritima [4] and T brucei PGK [3] the characteristic contacts formed...
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