... making more DNA, the processes of replication, and several ofthe applications related to DNA sequencing Then it’s off to RNAand protein synthesis We also spend some time talking about the Human ... such, there must be an associated equilibrium constant (K) The equilibrium constant in the preceding equation is Kw The value of Kw is the product ofthe concentrations ofthe hydrogen ion andthe ... physiological buffers In the human body, the pH of various body fluids is important The pH of blood is 7.4, the pH of stomach acid is 1–2, andthe pH in the intestinal tract is 8–9 If the pH of blood is more...
... G-tracts of one strand and only the 3¢-end G-tract ofthe other strand [30] (Fig 3D) The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one strand ... G-quadruplexes, there remain many challenges associated with the structure and molecular recognition in the human telomeres These include: (a) the structure and dynamics of all possible DNA, RNAandDNA ⁄ RNA ... to the situation for human telomeric DNA A Long human telomeric DNAandRNA sequences The next step toward understanding the structure ofthe ‘real’ telomeres is to address the question on the...
... amount ofthe RNA- RNA interactions contain compensatory base changes across the binding sites The algorithm presented herein is an extension ofthe PETfold algorithm [25] and makes further use ofthe ... description ofthe scoring of single-stranded positions However, they are scored the same way as in the original PETfold approach; for more details, see [25] The PETfold score is the sum ofthe evolutionary ... s1 and s … s , where s1 is a ncRNA and s is its target sequence For convenience, we adopt the convenk tion of RNAcofold and assume that the positions in s1 k k are numbered ≤ i ≤ | s1 | and the...
... years [2] The aim of this work to carry out homology modeling of both DNAandRNA polymerases from the linear mitochondrial plasmid of M perniciosa With the accomplishment of this work, these models ... observed in the whole structure As well as other polymerases from that family, this polymerase showed the three standard domains ofthe group: Palm, Fingers, and Thumb The active site oftheDNA polymerase ... structure oftheDNA ciosa mitochondrial plasmid polymerase from the M perniThe 3D The 3D structure oftheDNA polymerase from the M perniciosa mitochondrial plasmid Magenta: helices; yellow: strands;...
... emphasis on the induction ofDNA fragmentation, which is one ofthe most typical characteristics of apoptosis Regulators ofDNA fragmentation One ofthe terminal processes of apoptosis is DNA degradation ... They induce the permeabilization ofthe mitochondrial membrane with the release of cytochrome c andthe formation ofthe apoptosome [38] The major pro-apoptotic protein Bax exists mainly in the ... signal on and off Caspase activation is under the direct control of kinases and phosphatases, andthe indirect control of phosphorylation through the regulation of other apoptotic proteins Furthermore,...
... 589 and 597 within RGG3 are important for the binding of RGG3 to G-quadruplex DNAandRNA In ssDNA and ssRNA RGG3 (nM) Fraction ofRNA bound Fig Binding affinity of RGG3 to Htelo or rHtelo TheDNA ... poly G and poly U RNA in vitro [8] On the other hand, Hume et al [15] suggested that EWS binds to the proximal-element DNAofthe macrophage-specific promoter ofthe CSF-1 receptor gene The RGG ... previously [43] The 32 P-labeled 25-mer primer annealed to the 3¢ end ofthe template and could be extended by a DNA polymerase upon the addition ofthe dNTPs If complete extension ofthe primer occurred,...
... and mss are the intercepts and slopes ofthe lower and upper baselines ofthe melting curves, respectively; T is the temperature of interest in Kelvin, a is the molar fraction of strands in the ... sets ofthe temperature-dependent thermodynamics for the DNA/ DNAandRNA /DNA oligonucleotide duplexes In the present study, we determined the temperatureindependent and temperature-dependent thermodynamic ... As the enthalpy and entropy changes are state functions, their values, in nature, are dependent on the temperature of interest Table summarizes the thermodynamic parameters ofthe 24 DNA/ DNA and...
... Eqns (19) and (20), respectively m Thermodynamics ofthe binding of ADM and (1,10-phenanthroline)-copper to DNA Fig A comparison ofthe UV and visible spectrum of BLM-A5 after the cleavage of calf ... curves The molar ratio, r, ofDNA to L is defined as r ¼ nDNA;0 =nL;0 ¼ DNA 0 =½L0 ð17Þ where nDNA,0 and nL,0 are the initial amounts ofDNAand L, respectively Substituting Eqns (15) and (17) ... binds to DNAandDNA L the complex between DNAand L The intrinsic binding constant, KB, is defined by the equation [24,28,29]: KB ¼ y ð1 À yÞð DNA 0 À ny½L0 Þ ð14Þ Here, [DNA] 0 and [L]0 are the initial...
... distance of 50 mm underneath the dishes andthe particles passed through 50 mm of helium andthe thin mylar bottom ofthe culture dishes before entering the cells through the bottom ofthe dishes ... fraction ofthe DSB (about 1% of DSB induced by g-rays and about 10% by a-particles) are causing cell death On the other hand, the values of a for formation of chromosome fragments and colour ... problem The RBE value of 14.6 for cell reproductive death is similar to values in the range of to 15 published for many other lines of cultured cells [45] The RBE of derived for the induction of DNA- DSB...
... pathway to further our current understanding ofthe underlying mechanisms of AdbFGF-siRNA-induced growth suppression and apoptosis of glioma cells Materials and methods 2.1 Cell Culture and Adenovirus ... Interestingly, the expression of pSTAT3 Ser727 moderately decreased at 24 and 48 h and then restored to the control level at 72 h Furthermore, compared with the levels under the control and Ad-GFP ... http://www.jeccr.com/content/30/1/80 Page of Figure Ad-bFGF-siRNA reduces the activation of upstream molecules andthe expression of downstream molecules of STAT3 in U251 cells (A) Ad-bFGF-siRNA (MOI = 100) reduces the phosphorylation/activation...
... conceived the study, was leading the execution ofthe experiments, and participated in the writing ofthe manuscript K.S coordinated and supervised the study and was involved in the writing ofthe ... amounts of PCR products indicative ofthe presence of similar levels of these cellular RNAs in all three particle preparations These results suggest that packaging of these RNAs was independent ofthe ... independent of genomic RNA [28,32] Furthermore, the efficiency of encapsidation of most ofthe cellular RNAs seems to reflect their cellular abundance [28,32,33] One ofthe first cellular RNAs identified...
... The presence andthe therapeutic efficacy of anti-EGFR antibody in serum from theDNA vaccine group of mice The presence andthe therapeutic efficacy of anti-EGFR antibody in serum from theDNA ... naked DNA vaccine elicited Th1-bias immune response Hence, the choice ofthe route ofDNA immunizations and formulation ofDNA could thus represent an important element in the design of EGFR DNA ... with the design ofthe experiments and prepared the draft manuscript, MCY, CFT, CCW, PSL and HJY performed the experiments; CML provided advice on the design ofthe study and commented on the...
... Woods The Influence ofthe Ku80 Carboxy-Terminus on Activation ofthe DNA- Dependent Protein Kinase andDNA Repair is Dependent on the Structure ofDNA Cofactors In mammalian cells DNA double strand ... in which the initial radical induced on theDNA produces a single strand break The radical is then transferred to the other strand causing the second break7 Of these two possibilities the latter ... in which the initial radical induced on theDNA produces a single strand break The radical is then transferred to the other strand causing the second break7 Of these two possibilities the latter...
... involved in the maintenance ofthe methylation pattern during DNA replication where, the methylation ofthe parental strand is faithfully copied onto the daughter strand Two other families of enzymes ... discovered that they are involved in the longevity of Drosophila The intactness ofthe gene was required for the maintenance ofthe normal life span and over-expression resulted in prolonging the life ... al., 2001) 1.2 DNA methylation and cancer The analysis oftheDNA methylation profile, suggests the existence of several differences between normal and cancer cells Both sporadic and hereditary...
... access to the array and, if other clients had already referenced the data ofthe array, the physical memory cache ofthe array would be directly accessible to the client with no further message ... 5.5 Fault Handling The Mach page fault handler is the hub ofthe Mach virtual memory system The kernel fault handler is called when the hardware tries to reference a page for which there no valid ... entirely responsible for the initial values of this data andthe permanent storage ofthe data if necessary The Mach kernel makes no assumptions about the purpose ofthe memory object In order...
... worked in the communications sector Twenty years later, though the number of men remained unchanged, the number of women rose to 550 000 A similar situation was seen in the wholesale and retail ... 450 000 by 1995 The number of men grew only marginally from 425 000 to 480 000 over the same period In defence, the number of men declined from 225 000 to 200 000, while the number of women rose ... answer: The two decades between 1975 and 1995 brought significant changes in the representation of women in Freedonia's work force, according to the graphs In 1975, for example, some 300 000 men and...
... evidence is the lack of rigour and generalisability Furthermore, the magnitude ofthe effect ofthe MET may be influenced by institution-specific administrative features and policies Buist and coworkers ... of centres Before -and- after studies lack rigour and generalisability they are In the words ofthe slogan ofthe American Society of Critical Care Medicine, we need to deliver the ‘right care, ... lack of statistical power due to the large variance in outcome incidence and wide standard deviation andthe lower-than-expected incidence ofthe outcome measures under investigation Given the...
... ideas…related to the topic ofthe text drew the attention of 40% ofthe teachers and 12.5% ofthe students This shows that words and structures are of importance in avoiding the students’ misunderstanding ... in their mid-term and final exams for scholarship one hand and not re-taking the exams on the other hand Furthermore, the University of Odonto and Stomatology has been now co-operating with other ... true to the content field which forces learners to train themselves more outside the classroom to update them and meet the demand of their work Being aware ofthe great importance of ESP in the teaching...
... level of Proficiency at Hanoi University of Industry” is submitted in partial fulfillment ofthe requirements for the degree of Master of Arts is the result of my own work, except where otherwise ... improve the situation of teaching and learning speaking English to the year non-major English students would be made The thesis consists of three main parts: the introduction, the development, andthe ... the conclusion The introduction - gives rationale for the study and sets up the aims, scopes and methods ofthe study The develoment - which is considered as the main part ofthe study, includes...