... CW: Widespread genome duplications throughout the history of flowering plants Genome Res 2006, 16:738-749 The Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant ... counterpart Most of the genome shrinkage in Br could be explained by the deletion of roughly one-third of the redundant proteome as well as TEs in the euchromatic Br genome Only 14% of the Br genes ... indicates the genome split at around MYA (Figure 5g) Detection of a peak reflecting 3R in the Bo and Bna genomes but absence of this peak in the Br genome and between the other Brassica genomes...
Ngày tải lên: 09/08/2014, 20:20
... Although the size of the assembled genome increased by 29 Mb (12% of the version 4.0 genome) as a result of additional sequencing reads and better assembly, a total of 76 Mb of sequence (32% of the genome) ... level of resolution of the map (average 93 kb) This agreement could seem to be a circular argument as the map is the framework of the assembly This is not the case The genetic map and sequence ... organize them The eradication of errors in the map, even if it used the sequence to detect them and helped their resolution, was based on genetic methods (controls or addition of genotypes) Genome...
Ngày tải lên: 14/08/2014, 18:20
Analysis, sequencing and in vitro expression of PCR products
... of fragments are generated that all start at the 5′-end of the primer and extend to one of the A positions in the growing chain, and thereby correspond to each T in the template strand When these ... Comparing the migration rates of the fragments from the different reactions allows the sequence of the DNA to be read starting with the fastest migrating fragments that are closest to the primer ... not the touch the side of the tube with the DNA Wash the pellet by adding 100 µl ice-cold 70% ethanol, vortex briefly and centrifuge as before with the tube in the same orientation Aspirate off the...
Ngày tải lên: 25/10/2013, 22:20
Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
... observed in the cytoplasm of the cells The staining appears to be specific of the probe sequence because the staining is clear with the complementary sequence to RpCAbr, but not with the sense probe ... amplifications of RpCAbr and RpCAtr with 18S amplification The number of cycles on the y-axis is the difference between the number of cycles required to amplify each transcript and the number of cycles ... expressed in tissues of the worm These sequences are also compared with other CA sequences from vertebrates and nonvertebrates Results CA sequences from the SSH libraries From the body wall-subtracted...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt
... brain of rainbow trout consists of a subunit of 55 kDa [9] Therefore, the 55-kDa protein was assumed to be a single subunit of Nile tilapia anserinase As the molecular mass of Purification and sequence ... anesthesia (50 mgÆL)1 of benzocaine) and tissues were then collected Total RNA was prepared from tissues of the fish using the TRIzol Reagent, and the first-strand cDNA was synthesized using the ... in which homologous sets of the proteins of interest are grouped into families, and the homologous families are grouped into clans [19] Therefore, the blastp program of the MEROPS database was...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx
... effect of the length of these regions on the distribution The intronic and intergenic regions were then classified on the basis of their lengths Within each of the length groups, the numbers of LIRs ... none of the others They are labeled as rare LIRs, accounting for 49% of all the LIRs in the human genome We explored the LIRs in the NPS-Septin gene family in more detail A blat search in the ... Although there are also a large number of (CA)n and (GA)n repeats in the human genome, the frequency of their complementary repeats (TG)n and (CT)n is much lower [37] Therefore, the presence of the...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
... proceeds until the end of the D-loop, overlapping with the HSP [19,20] In the mouse system, the 3¢ end of the D-loop H-strand RNA maps to the CAA sequence ending at nucleotide 16 295 of the genome ([20] ... of as yet unknown function [19,20] The 5¢ end of this RNA maps to nucleotide 15 417 of the mouse genome, which marks the start of tRNAPro on the L-strand The 3¢ end maps to nucleotide 16295 of ... protein bound to the H and L-strands of the probe Some of the discrepancies between the South-western and UV cross-link analyses as well as the failure to obtain the sequence of the poly(vinylidine...
Ngày tải lên: 08/03/2014, 08:20
The Selection, Use and Maintenance of Personal Protective Equipment (PPE) potx
... location of the piece of equipment; • Particulars of the equipment; • Date of examination/test; • Signature of the person who carried out the test; • Description of the condition of the equipment, ... tested) Breakthrough time – the time between initial contact of the chemical on the surface of the glove and the analytical detection of the chemical on the inside of the glove Given on a chemical ... into account: • the toxicity of the agent • the size of the particle • the amount of movement involved in the task and working conditions • the individual, e.g face shape, presence of beard, glasses...
Ngày tải lên: 08/03/2014, 13:20
Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx
... collected from another region of Crete, and this residue substitution was confirmed There are no N-glycosylation sites in the deduced sequence Quality of the model The high quality of the collected ... represents the concentration of charybdin that inhibited in vitro protein synthesis by 50% DNA sequence and derived amino-acid sequence The DNA sequence and the derived amino-acid sequence are ... activity depends on the source of the RIP and of the ribosome There are many reports that RIPs induce apoptosis [2,3] The main application has been focused on the construction of chimeric molecules...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Natural and amyloid self-assembly of S100 proteins: structural basis of functional diversity doc
... maintaining the dimeric state of the S100 proteins The residue invariability and the conserved spatial arrangement of the helices at the dimer interface are the basis for heterodimer formation In the ... binding of Zn2+ to the low affinity site triggers the formation of a tetramer via the assembly of two S100A2 dimers [15] Together, these results point to a very clear role of metal ions in the formation ... presumably a result of the general destabilization of the protein Further contributions might come from the exposure of a hydrophobic surface upon Zn2+ binding, making additional exposure of the hydrophobic...
Ngày tải lên: 23/03/2014, 03:20
Báo cáo khoa học: Cytoskeleton-modulating effectors of enteropathogenic and enterohaemorrhagic Escherichia coli: Tir, EspFU and actin pedestal assembly pdf
... bacterial attachment to the surface of epithelial cells and the effacement of microvilli [5,6] The formation of these ‘attaching ⁄ effacing’ lesions is critical for the pathogenesis of EPEC and EHEC, ... which these sequences activate N-WASP The first half of each EspFU repeat forms an a helix that mimics the connector portion of the WCA domain of N-WASP, which is normally involved in binding to the ... Notably, all of these factors may interact with EspFU [62], suggesting that they are not mere bystanders during EHEC infection of host cells Thus, the ability of EspFU to alter the activity of these...
Ngày tải lên: 29/03/2014, 09:20
compact heat exchangers selection design and operation
... account In the fourth chapter the implications of compactness are examined analytically, from the point of view of their impact on the size and shape of one side A feature of this chapter is the separate ... Heat of reaction will then be added or removed as appropriate by the other fluid, but without any control of the rate of removal, which ot~en depends on the rate of mixing The effectiveness of ... Another, more recent derivative of the PHE is the welded plate exchanger, which utilises speeialised seams to enable the welding of the plates together either as pairs or as a whole unit These...
Ngày tải lên: 02/04/2014, 15:23
emulsions and oil treating equipment selection, sizing and troubleshooting
... elimination of the choke, used to control the flow rate of a well, is not a solution to the problem The degree of agitation and the nature and amount of emulsifying agent determine the “stability” of the ... to the bottom of the tank Thus, the greater the difference in density between the oil and water phases, the easier the water droplets will settle 1.3.4 Size of Water Droplets The size of the ... is a result of the decrease in viscosity of the oil phase 1.3.8 Water Salinity The salinity of the water is a measure of the total dissolved solids in the water phase As salinity of the water increases,...
Ngày tải lên: 02/04/2014, 16:03
wiley finance, investment manager analysis - a comprehensive guide to portfolio selection, monitoring and optimization [2004 isbn0471478865]
... 107 or 108 of the 1976 United States Copyright Act, without either the prior written permission of the Publisher, or authorization through payment of the appropriate per-copy fee to the Copyright ... a result of scheduling, information delays, and a variety of other factors OUTLINE AND STRUCTURE The structure of this book is designed to take the reader through all the stages of the investment ... ANALYSIS Example of Investment Objective The financial objectives of the plan are based on a comprehensive evaluation of the capital markets in the context of modern portfolio theory and have been...
Ngày tải lên: 08/05/2014, 10:12
Báo cáo hóa học: " Research Article Carrier Frequency Offset Estimation for Multiuser MIMO OFDM Uplink Using CAZAC Sequences: Performance and Sequence Optimization" ppt
... the idea to the estimation of multiple CFO values in the uplink of multiuser MIMOOFDM systems Let the training sequence of the first user be s1 The training sequence of the mth user is the cyclic ... freedom in the sequence construction The S&H sequences have the largest number of degree of freedom in the construction of the CAZAC sequences However, the simulation results show that they have ... Chu sequences, there are a finite number of sequences for each sequence length Therefore, the optimal sequence can be obtained using a search among these sequences However, for S&H sequences, there...
Ngày tải lên: 21/06/2014, 05:20
Báo cáo hóa học: "Research Article End-to-End Joint Antenna Selection Strategy and Distributed Compress and Forward Strategy for Relay Channels" potx
... constraint P across m antennas of stage n Therefore with AF by each antenna subset on the chosen th path, the received signal at the SkN subset of antennas of the ∗ destination at time t + N of ... subset of antennas of stage n, n that is, Skn ⊂ [Mn ] Let eSkn Skn+1 be the edge joining the set of antennas Skn of stage n to the set of antennas Skn+1 of stage n + 1, where |Skn | = m, ∀, n Then ... of κ + independent paths Then each relay node knows on which of the paths of S it lies, and depending on the index of the element of S from the destination, it knows whether to transmit or remain...
Ngày tải lên: 21/06/2014, 20:20
Automotive Transmissions Fundamentals, Selection, Design and Application
Ngày tải lên: 24/06/2014, 09:26
ASAP: Amplification, sequencing and Annonation of Plastome pot
... thành thực Phương pháp tập trung khái thác vùng mã hóa có độ bảo tồn cao chèn nằm gene plastid Theo đó, trình tự plastid loài hai mầm xếp thẳng hành nhằm dò tìm vùng bảo tồn Sau cặp mồi anh em...
Ngày tải lên: 01/07/2014, 18:20