the gene expression omnibus
Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
Ngày tải lên :
03/11/2012, 11:44
... repressed expression of the ZCD1 gene C) a repressed expression of BSG gene It is coincidence that the detected genes, the PKN1 and BSG gene cluster on the adjacent chromosomal location: The gene expression ... validated genes (A, B, C) The graphs display the results of the quantitative PCR of the differential gene expression related to the HPRT1 gene in HeLa cells 24h after treatment; the abscissae show the ... of the affected genes GO terms (GO: 24 http://www.geneontology.org/) of the differentially expressed genes are shown in table Figure Comparison of the expression level of the reference genes The...
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Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx
Ngày tải lên :
28/03/2014, 22:21
... of SREBP gene expression is not the initial trigger for the glutamine effects If induction of the SREBP gene expression serves as the initial trigger for the glutamine effects, the gene expression ... imply the involvement of the O-glycosylation of Sp1 in the induction of SREBP-1a transcription by glutamine The gene expression of SREBP-1c, which represented less than 20% of the SREBP-1a gene expression ... affects the expression of the genes involved in cholesterol homeostasis The results clearly demonstrate that glutamine stimulates SREBP processing and the gene expression of SREBP targets The same...
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Báo cáo hóa học: " Changes in the gene expression profile of Arabidopsis thaliana after infection with Tobacco etch virus" potx
Ngày tải lên :
20/06/2014, 01:20
... this gene may speed up cell division The second gene, At5g04560, encodes the DME DNA glycosylase that activates the maternal MEA allele in the endosperm The third gene, At5g64630, encodes for the ... computing the log2 of the ratio between the gene expression level in TEV-infected plants and control plants (mock inoculated) and normalized by the number of genes in the set To minimize the number ... interested in knowing whether the overall pattern of gene expression was significantly affected by TEV infection To so, an ANOVA model in which gene (the 13,722 valid genes in the dataset) and treatment...
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Báo cáo hóa học: " The effects of hypertonic fluid administration on the gene expression of inflammatory mediators in circulating leucocytes in patients with septic shock: a preliminary study" pptx
Ngày tải lên :
20/06/2014, 23:20
... used to make cDNA and then the ratio of the Housekeeper gene to the gene of interest was used Because the housekeeper gene is not affected by the treatment, the Ct and the efficiency of amplification ... (PMN) were present (Table 2) Gene expression at baseline The expression at baseline of all measured mediators was comparable between the two groups (Figure 1) The genes Page of Table Baseline ... in the peripheral blood is that only the endocrine overflow is measured, not the local autocrine and paracrine receptor binding effects [10,11] On the other hand, measuring expression of the...
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báo cáo khoa học: " HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers" docx
Ngày tải lên :
11/08/2014, 11:22
... and down-regulated genes both in the leaf and flower samples (Table 2) Similar expression data was obtained for these selected genes using both these methods The construction of the microarray probes ... impair photosynthesis, and thereby lead to sugar starvation and further on, to altered regulation of the metabolic stress-related genes The general stress response observed in the HC-Pro expressing ... variable, but it often differed from the wild type The petals were often fused together and the color of the petals was changed from pink to pale pink or variegated The anther filaments were often converted...
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Báo cáo y học: "The gene expression profile of preclinical autoimmune arthritis and its modulation by a tolerogenic disease-protective antigenic challeng" ppt
Ngày tải lên :
12/08/2014, 18:20
... agreement of the expressions obtained with the two methods As Inc phase of AA revealed the most marked differences in gene expression, we chose this phase to further study the gene expression profiles ... was used to assess the agreement in gene expression obtained with microarrays and qPCR for the selected genes Results The gene expression profiles at different phases during the natural course ... representative genes was generated in the program ‘R’ with the package 'gplots' Specifically, the fold change of expression levels in log2 scales were organized by the Expression Profiler software using the...
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Báo cáo y học: " Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells" doc
Ngày tải lên :
13/08/2014, 09:20
... contribute to the variable effects of prostratin in the absence of Tat when viral gene expression is low The overall outcome of viral gene expression observed in this study may represents the net result ... functions for HIV-1 gene expression as inferred from infections with the Tat+ and Tat- reporter viruses We therefore wished to investigate the global effects of prostratin on cellular gene expression ... luciferase expression in the Tat- virus These data indicate that in the absence of Tat, prostratin has small and variable effects on reporter virus expression, consistent with the proposal that the...
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Báo cáo sinh học: "The recombinant adeno-associated virus vector (rAAV2)-mediated apolipoprotein B mRNA-specific hammerhead ribozyme: a self-complementary AAV2 vector improves the gene expression" docx
Ngày tải lên :
14/08/2014, 19:22
... Geoscan Enabler, the image was captured using Adobe Photoshop 5.0, and the background was removed with the guidance of the stereomicroscope The total areas of the aorta and the atherosclerotic ... of atherosclerotic lesions The mice were anesthetized, exsanguinated and the aorta was carefully excised with part of the heart still attached Under the stereomicroscope (Leica MZ60), all the ... removed With the major branching vessels still attached, the aorta was opened longitudinally from the iliac bifurcation to the aortic arch, and all the branching vessels and the heart were then removed...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
Ngày tải lên :
16/02/2014, 15:20
... whether ASF ⁄ SF2 might modulate the expression of ARE-containing genes Accordingly, we tested the effect of overexpressing ASF ⁄ SF2 on the expression of Renilla luciferase (Rluc) reporter genes ... oxidative stress was a result of the relocalization of the protein and not an increase of ASF ⁄ SF2 gene expression (Fig 4A) These data are further supported by the capacity of ASF ⁄ SF2 to migrate ... direct repeats in the 3¢ UTR These reporter genes were placed under the control of a bidirectional cytomegalovirus promoter mediating the transcription of another reporter gene encoding firefly...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
Ngày tải lên :
19/02/2014, 05:20
... treatment, neither treatment with the E-cad2 antibody nor the EGFR antibody induced Erk activation (Fig 2E) Taken together, these results suggest that the observed Erk activation was specific for the disruption ... [50] However, the site at which the PI3K signaling feeds into the Erk activating pathway varies in these systems: at the Ras, Raf or MEK activation level In the Decma-induced pathway, the signal ... (Fig 1A, B,D) To determine whether the disruption of cell–cell adhesion by Decma influenced expression of the uPA gene, we examined change in uPA mRNA levels Northern blot analysis showed only...
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Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt
Ngày tải lên :
19/02/2014, 17:20
... platform spread on the b-sheet from b4 to b2 in the 5¢)3¢ direction More often, the nucleotide at the 5¢ end of the central dinucleotide contacts the loops at the bottom of the b-sheet (loop and ... [31], with any type of nucleotide either at the 5¢ or the 3¢ position The nucleotides at these two positions always adopt an anti conformation, except for the G at the 3¢ position always found in ... is provided by other non conserved elements of the RRMs The two most frequently observed elements are the protein side chains at the surface of the b-sheet (RNP position and the two adjacent...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
Ngày tải lên :
21/02/2014, 01:21
... signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in growing cells treated either with NAMI-A or the MAPK/ ERK inhibitor PD98059 The addition of ... c-myc gene expression (Fig 5C) Furthermore, NAMI-A addition to PD98059-treated cells failed to produce any additive inhibition of the residual c-myc gene expression induced by serum, suggesting the ... c-myc gene transcription and protein expression To establish whether the decrease in c-myc mRNA expression elicited by NAMI-A might have occurred at the transcriptional level, we assessed the rate...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... factors can influence gene regulation ABPs transduced the changes in cell structure that occur during morphogenesis to the nucleus, resulting in changes in gene expression via either the nuclear shuttling ... proliferator-activated receptor-c Among these, the AR is the most widely studied and well-characterized The AR is a ligand-activated transcription factor that controls the expression of genes involved in functions ... and enhances the ability of Nkx2.5 to bind to the NKE (Nkx2.5-binding element) boxes in the ANF promoter These results suggest that hhLIM promotes specific expression of the ANF gene by cooperating...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
Ngày tải lên :
07/03/2014, 05:20
... transfected with the NT siRNA (Fig 9, columns 11 and 12 versus and 10) Therefore, it seems that the longer the transfection period, the stronger the off-target effects of siRNA on gene expression mRNA ... the expression level of the RNAi pathway components in each cell type and ⁄ or by different transfection efficiencies These hypotheses highlight the importance of the cellular environment in the ... SREBF1/siRNA1 20 genes genes NT siRNA 10 genes genes B Transfection 0h Extraction h post-T 24 h Extraction 24 h post-T 48 h 72 h SREBF1/siRNA1 26 genes 12 genes NT siRNA 27 genes 15 genes Fig Effect...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
Ngày tải lên :
16/03/2014, 02:20
... including the synthesis of albumin, lipoprotein and several other liver-specific functions, and, most importantly, these functions are stable through passages They are therefore valuable in the study ... regulate, at least in part, the expression of gluconeogenic genes [17,31], we studied its status within the cell in the given experimental conditions Foxo1, another member of the forkhead family of ... F1,6bpase, and the targeted overexpression or knockouts of these enzymes play a major regulatory role in glucose homeostasis [40,41] As far as the regulation of these genes is concerned, the Foxa family...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
Ngày tải lên :
16/03/2014, 16:20
... the expression of the gene coding for this receptor, Northern blot experiments were performed The results revealed that the expression level of the glucanbinding protein at the beginning of the ... strong increase in the expression of SDFIBI occurs Likewise, a strong expression pattern was determined for the SDEGFI-PREC gene (Fig 6) The level of expression of the housekeeping gene, tubulin, ... strengthen the conclusions, in situ hybridization studies were performed, which again show that the expression of this gene is primarily seen in the cells lining the canals – the pinacoderm From these...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
Ngày tải lên :
17/03/2014, 10:20
... inserted and the resulting interrupted genes were cloned into the suicide vector, pKAS46 These constructs were then introduced into the chromosome of RAS46 by side-specific recombination The respective ... detector and the reduction of the flavin moiety of the protein was monitored at 450 nm The inset shows the difference spectrum; the fully oxidized spectrum at 10 was corrected vs the reduced spectrum ... oxidoreductases of the inside-out vesicles The reduction rate then decreased slowly until the unspecific rate of the background decline was again reached (Fig 2A) Subsequent addition of external NADH to the...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... observed at the beginning of the S phase (0 h), and these marks were attenuated for the rest of the cell cycle (10 and 20 h), correlating with the expression of the gene (Fig 6C,D) The correlation ... with Hox gene expression indicates the critical roles of MLL1 and H3K4 trimethylation in differential Hox gene expression during the cell cycle To further confirm the importance of MLL1 in the regulation ... HoxA10 gene promoters was also correlated with their respective expression profiles (compare Fig 6A,B with Fig 6C,D) In the case of the HoxA7 gene promoter, the recruitment of RNAP II and MLL1 and the...
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A gene expression database for the molecular pharmacology of cancer pptx
Ngày tải lên :
30/03/2014, 13:20
... measured gene expression levels The gene expression profiles show considerable coherence, in that cells clustered on the basis of their expression profiles for 1,376 genes and 40 targets tend to sort themselves ... a gene and a drug by taking the (normalized) level of expression of the gene in each cell line, multiplying it by the corresponding (normalized) sensitivity of the cell to the drug, summing the ... based on their activity patterns across the 60 cell lines b, Cluster tree of the 118 drugs based on the correlation of their activity patterns with expression patterns of the genes The distance...
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Báo cáo khoa học: Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation pot
Ngày tải lên :
30/03/2014, 20:20
... considerable up-regulation of the expression of the genes for selenoprotein M and spicule-associated protein was detected (Fig 8) On the basis of the data obtained, the steady-state expression was almost ... somites, the spinal cord and the axial fin fold [21] These data interestingly show that the expression pattern of the different selenoproteins in the fish is region-specific To obtain an insight into the ... Hydropathicity plot of the S domuncula SPIaP_SUBDO; the calculation was performed by the method of Kyte and Doolittle [51] The horizontal axes show the amino acid numbers along the protein vs the corresponding...
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