the fear of death follows from the fear of life

... from cancer at any stage of the disease with the hope of raising awareness of the types of therapies that may be appropriate and increasing awareness of the role of the pain specialist in cancer ... towards the end of life, but also pain at diagnosis, as a consequence of cancer therapies and in cancer survivors. The document relates the science of pain to the clinical setting and explains the ... understanding of their condition. ã what the pain means to the individual and their family. ã how the pain may impact upon relationships within the patients family. ã whether the pain inuences the patients...

Ngày tải lên: 14/02/2014, 21:20

... from the same volume of whole serum with QIAmp Blood Midi Kit (QIAGEN, Valencia, CA) according to the manual. Bacterial DNA was also extracted from the bac- terial particles trapped from the ... spe- cific for 16S rRNA, another one – with the primers for ompA in nested PCR format. As can be seen from Figure 2, the sensitivity of PCR reaction was similar regardless of the primer set used. ... may originate from destructed mononuclear cells and/or macrophages residing in the atherosclerotic plaques. However regardless of the origin, we can claim that the presence of virulent chlamydial...

Ngày tải lên: 26/10/2012, 08:57

Ngày tải lên: 05/09/2013, 16:10

... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro- teins. The ... comparable with the two-metal-ion mechanism of both RNA and DNA synthesis [17]. In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plas- mid pIT3 isolated from ... account for the promiscuous nature of the synthesis functions performed by the prim–pol domain and to discriminate between the functions of in vitro primase and polymerase. Another finding of our biochemical...

Ngày tải lên: 18/02/2014, 18:20

... cleft, the putative catalytic zinc being at the bottom of the cleft and a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8]. Grad- ual depletion of the ... Novagen. Expression of Pt-gdh under the control of araB promoter was induced for 4 h at 30 °C by the addition of 0.2% ara- binose when the A 600 of the growing culture reached 0.5. The cells from a 1-L ... tested, i.e. from 0.05 to 1 mm. The influence of Zn 2+ on the pH stability of GdhA is most evident after incubation (1 h, 55 °C) at pH 3.5, where, in the presence of the metal ion at 0.1 mm, there was...

Ngày tải lên: 19/02/2014, 16:20

... transfer from CDH to cytochrome c, Phe166, which is located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the ... the special features of this enzyme. The optimum pH values of flavin reduction by cellobiose (pH 4.5–5.0) and of electron transfer from flavin to heme (pH 3.5–4.0) differ from each other, and the rate-limiting ... This was expected from the thermodynamic result that the redox potential of heme in F166Y was lower than that of WT. However, when the electron transfer rate k (s )1 ) and the driving force DG...

Ngày tải lên: 19/02/2014, 18:20

... their positions. The arrowheads depict the residues important for the structural core of the IL-10 gene. The underlined amino acid residues are the signal sequences of the respective genes. The ... analysis investi- gated. This work will pave the way for further investigation of the biological function of this gene, and the probability of the presence of IL-10-related genes in fish, as seen in mammals. Acknowledgements This ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...

Ngày tải lên: 20/02/2014, 02:21

... 1). Subsite mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (k int ) in the productive complex is independent of the length of the substrate, K m and ... Laboratory of Microbiology, Wageningen University, the Netherlands 2 Laboratory of Food Chemistry, Wageningen University, the Netherlands Pectin is a complex polysaccharide present in the cell wall of ... (1973) Subsite affinities of glucoamylase: examination of the validity of the subsite theory. Biochim Biophys Acta 302, 362–375. L. Kluskens et al. An exopolygalacturonase from Thermotoga maritima FEBS...

Ngày tải lên: 20/02/2014, 03:20

... (e.g., the height of a person), while for others it is only the center of a distribution (e.g., the weight of a coffee cup). Questions with no trusted and exact answers were eliminated. From the ... information for the requested object, the requested object will receive the average of the extracted values of the whole set of the retrieved comparable objects and the com- parison step will be essentially ... value for the given object. During the first stage it is possible that we directly extract from the text a set of values for the requested object. The bounds processing step rejects some of these...

Ngày tải lên: 20/02/2014, 04:20

... years ago and supports the notion of a universal occurrence of hemocyanins in the Myriapoda [15,16]. At least three hemocyanin subunits were present at the time of divergence of the Chilopoda and ... the determination of the N-terminal sequences, C. Hunzinger for the MALDI-TOF analyses, C. Bache and J. Hermanns for their help with the cloning experiments, and J. R. Harris for correcting the ... kDa, the isoelectric points (pI) range from 5 to 6.5 (Fig. 2). MALDI-TOF analyses of tryptic peptides show that the spots represent four distinct subunit types, as deduced from the pattern of identical...

Ngày tải lên: 20/02/2014, 11:20

... be collected from each seed word, and then checked whether each of the target terms was included in the system output. We counted the number of tar- get terms in the following five cases. The right ... total; the average number of output terms per input is 12.2 terms. We checked whether each of the 610 terms is a correct related term of the original seed term by hand. The result is shown in the ... term. 3. There is a definition or explanation of the term. 4. There are several technical terms that are re- lated to the term. We have implemented the checking program of the first two conditions in the...

Ngày tải lên: 20/02/2014, 16:20

... Rejoiced Branch Rickey and the Or igins of the Breaking of the Color Line He really leads a double life – one with his conscience and the other with the employer who pays him one of the top salaries in ... was good, for the owners both of the Negro team and the local team. The attendance for the annual game was often the highest of the year. ✶ Parallel with serious Negro ball were the black clown ... Introduction Jerry Malloy 1 The Birth of the Cuban Giants: The Origins of Black Professional Baseball Lee Lowenfish 15 When All Heaven Rejoiced: Branch Rickey and the Origins of the Breaking of the Color Line...

Ngày tải lên: 21/02/2014, 06:20

... on the same plot of the values found for de novo synthesis in the presence of 7.5 or 2.5 m M MnCl 2 with various amounts of EDTA, or in the presence of MnCl 2 only [11], as a function of the ... where the authors have identified the reverse products and shown that the CMP activation of the reverse of synthesis is by a different mechanism from base exchange [20]. Further analysis of the ... showing that the endogenous level of CDP-DAG is too low to allow detectable synthesis. The level of PtdIns in the membranes does not allow synthesis of detectable PtdIns by exchange of the polar...

Ngày tải lên: 22/02/2014, 04:20

... quality model to estimate the dispersion function, the accuracy of the estimated emissions will depend on the ability of the model to reproduce the dispersion of the pollutants. Until now, ... observed at this period of the day. From 2 pm to 6 pm, the wind direction is oblique to the street axis and the wind speed is higher than in the morning. At these hours of the day, tracer ... which, C is the concentration of a particular pollutant in the street (g/m 3 or mg/m 3 ); E is the emission of the pollutant from road traffic in the street; F is a function describing the dispersion,...

Ngày tải lên: 05/03/2014, 16:20

... determines the diagnosis of TB for the majority of adults, positive culture results often are lacking for children. In the majority of cases, the diagnosis of pediatric TB is established by the triad of ... and analysis of epi- demiologic and other data ã Development of policies and procedures and of a plan for controlling TB, on the basis of the assessment of the problem ã Assurance of diagnostic, ... health, their skills and capacities, the places where they work, the way they are organized, the equipment and systems available to them, and the fiscal resources they command) represents the public...

Ngày tải lên: 06/03/2014, 04:20

... the finding that at pH 10 and 75 °C, the inactivation of b-glucosidase also does not involve any loss of secondary structure [1]. Conclusions The stability of the b-glucosidase from the hyperther- mophile ... 113 of the large volume change associated with the forma- tion of a free charge [30]. Hence, a reduction in the number of electrostatic interactions would increase the pressure stability of the ... addi- tion, the band at  1.0 GPa in Fig. 5C does not resemble the broad, featureless band typical of an unfolded protein. Taken together, these observations suggest that the unfolding at the level of the...

Ngày tải lên: 07/03/2014, 03:20

Ngày tải lên: 07/03/2014, 05:20

Ngày tải lên: 07/03/2014, 05:20

Ngày tải lên: 07/03/2014, 10:20

Ngày tải lên: 07/03/2014, 15:20