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the experimental far ir and far raman spectra

Development of multivariate curve resolution and associated system identification tools for IR emission, chiroptical, and far infrared and far raman spectroscopies

Development of multivariate curve resolution and associated system identification tools for IR emission, chiroptical, and far infrared and far raman spectroscopies

Cao đẳng - Đại học

... (a) experimental Far- IR spectra of Mn2(CO)10 and (b) experimental Far- Raman spectra of Mn2(CO)10 122 Figure 5.3 (a) experimental Far- IR spectra of Re2(CO)10 and (b) experimental Far- Raman spectra ... Data Analysis: The Application of BTEM to IR and Raman Spectroscopic Data 120 5.3 Results: The Experimental Far- IR and Far- Raman Spectra 122 5.3.1 Experimental Data Sets 122 5.3.2 Spectral Reconstruction ... pure component spectra (●) ,UV-Vis pure component spectra (Δ) and experiment design (○) 110 Figure 5.1 (a) experimental Far- IR spectra of Mo (CO)6 and (b) experimental Far- Raman spectra of Mo...
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Tài liệu Selecting the Top n Rows in a DataTable doc

Tài liệu Selecting the Top n Rows in a DataTable doc

Quản trị mạng

... several steps First, sort the DataView on the Freight field in descending order; this places the top n records at the top of the view Next, get the Freight value for the nth record and set the DataView ... as is the case with Freight In this case, iterate over the records following the nth record and add criteria to a copy of the data view filter to exclude them from the view Use either the primary ... where there might be duplicate values in the nth record This would be used instead of the technique just outlined However, the technique shown requires no sort other than on the top n column The...
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Tài liệu Murray N. Rothbard - History Of Money And Banking In The United States (pdf) pptx

Tài liệu Murray N. Rothbard - History Of Money And Banking In The United States (pdf) pptx

Ngân hàng - Tín dụng

... knows about the way in which people value different conditions, about their wishes and desires and their plans to realize these wishes and desires It is the knowledge of the social environment in ... economic interests The ideology is the smoke screen for their loot, the fictitious clothes spun by the intellectuals to hide the naked plunder of the Emperor The task of the historian, then, is to penetrate ... scrutinize the goals and actions of the large Wall Street commercial and investment bankers, their industrial clientele, and their relatives and allies in the political arena Rothbard’s analysis of the...
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Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx

Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx

Kĩ thuật Viễn thông

... = 150 N Problem 2-17 Determine the magnitude and direction of the resultant force F R Express the result in terms of the magnitudes of the components F and F and the angle φ Solution: F R = F ... Problem 2-20 The plate is subjected to the forces acting on members A and B as shown Determine the magnitude of the resultant of these forces and its direction measured clockwise from the positive ... = 561 lb Problem 2-22 Determine the magnitude and direction of the resultant FR = F + F2 + F of the three forces by first finding the resultant F' = F1 + F and then forming F R = F' + F Given:...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Báo cáo khoa học

... targeting to the microsomes requires the cotranslational insertion of the newly synthesized protein into the microsomal membrane, where the N-terminal hydrophobic signal sequence of the protein ... in the association of the translational complex with the microsomal membrane [13,14] Thus, the N-terminal hydrophobic sequences of CYPs are important for their targeting to and retention in the ... single N-terminal membrane anchor and most of the remaining protein exposed to the cytosolic side [15,17,30,31] The intramitochondrial localization of CYPs and their topologies were studied using...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

Báo cáo khoa học

... ultrafiltration with the chelating buffer at pH and then diluted to the appropriate concentration The other part was diluted directly into buffer with 50 lm CuSO4 The far UV CD spectra of stefin B ... rectangular cell, bandwidth nm, each nm for s) (B) Far UV CD spectra of stefin B variant (Y31 isoform) in the presence and without Cu2+ (C) Far UV CD spectra of the P79S mutant in the presence and without ... UV CD spectra in Fig 4A reveal small differences in intensity and shape between stefins A and B and the P79S mutant The two isoforms of stefin B have exactly the same far UV CD Regardless of the...
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Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc

Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc

Báo cáo khoa học

... representation of the pAO1 gene and ORF cluster flanked by Tn554 and DTn The cluster consists of the pmfR gene, encoding the regulator of the purU-mabO-folD operon, a permease-like ORF, the genes of the purU-mabO-folD ... of the monoamine oxidase (mao) and the succinate semialdehyde dehydrogenase (sad)-like genes The pAO1 DNA carrying the corresponding ORFs was amplified with the primer pair #1 and #2 for mao and ... Prosite PS00070) in the form of GEAC304TAAN The unexpected finding that CH3-4-aminobutyrate and not 4-aminobutyrate was the substrate of the AO and thus both MABO and AO have the same substrate...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

Báo cáo khoa học

... indirect effect on the binding environment of fosfomycin The direct influence comes from two hydrogen bonds formed by the nitrogen of the N-acetyl group and the 3¢-oxygen to the phosphonate and ... green and the nitrogen atoms of R120 and K22 in blue The open form of MurA is unliganded (A) and the closed conformation (B) contains fosfomycin (orange) and UDPNAG (purple) in the active site The ... change upon ligand binding The loop region of the upper domain moves towards the active site of the enzyme, thus shielding the substrates (and ligands) from bulk solvent A detailed thermodynamic...
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Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx

Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx

Báo cáo khoa học

... cells and their N-glycans were processed before they arrived at the cell surface To determine the influence of N-glycosylation on the quality control of GAT1, the half-life of GAT1 wild type and ... mutants (with the exception of DDQ) gave two bands, while plasma membrane proteins gave only one large band (Fig 3A) On the basis of the Endo H digestion (Fig 2B,C), the large band is assigned ... mature complex type and the small band to the proteins with N-glycans of mannose-rich type All bands of the mutants had reduced molecular mass, compared with that of wild type NNN The reduced molecular...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

Báo cáo khoa học

... determine whether the matrix defect could be the consequence of precocious impairment in the inner membrane The decrease in enzymatic Lon protease activity may be the result of either a loss of ... acid [40,41] They are prime target of ROS due to their location near to the site of ROS production [42] and could be the source of carbonyl adducts Other recent reports have shown that there is intracellular ... from 10-month-old (lane 1) and from 27-month-old rats (lane 2) Standard molecular masses (lane 3) The arrow and arrowhead show the disappearance and appearance of bands, respectively Ó FEBS...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf

Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf

Báo cáo khoa học

... in the N-domain and the other in the pore of the ClpA hexamer In the case of short unstructured peptides or unfolded proteins such as casein, binding to the tyrosine residues in the hexameric pore ... interaction between ClpS and the N-domain of RR ⁄ AA, and this result suggests that neither the local nor the overall structure of the RR ⁄ AA mutant was compromised Mutation of the conserved arginine ... the standard error of the mean A non-specific protein band is indicated by an asterisk (D) The functional interaction between ClpS and ClpA (wild-type and RR ⁄ AA) was observed by monitoring the...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc

Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc

Báo cáo khoa học

... during the injection phase was the largest and there was low dissociation after the end of injection Discussion The main hypothesis for pathology in AD and other neurodegenerative diseases is the ... at desired concentrations in a mL cuvette and stirred at 25 °C Vesicles were added at the required concentration and the time course was followed for 30 The permeabilization induced by the proteins ... mol) The results are mean ± SD, n ¼ 1–4 The degree of permeabilization is expressed as the percentage of the maximal value obtained at the end of the assay by the addition of mM Triton X-100 The...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx

Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx

Báo cáo khoa học

... CiÆmmol)1; DuPont NEN) [18] as the tracer ligand Binding data were analyzed by nonlinear regression to fit theoretical Langmuir binding isotherms to the experimental data using the FIG P program (Biosoft, ... a-helical transmembrane domains traversing the lipid bilayer The enlarged circle shows the amino acid sequence of the distal N-terminus of the V1aR and indicates the position of Arg46 that was subjected ... for each of the 19 mutant constructs and the dose–response characteristics compared to the wildtype V1aR None of the 19 encoded amino acids could substitute the Arg46 and retain the wildtype...
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Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf

Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf

Báo cáo khoa học

... then added to the immobilized fusion protein The N-terminal NBD passed through the column, and was present entirely in the flow-through fraction, demonstrating that under the conditions used there ... Our results show that the N-terminal and C-terminal NBDs are functionally similar in their ability to bind ATP and ADP, but show some differences in their ability to bind other nucleotides, including ... part of the kinetics of the transport cycle of the intact transporter Furthermore, the data demon- strate that the two NBDs interact in a similar manner with 2-deoxy-ATP and CTP Lastly, the data...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt

Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt

Báo cáo khoa học

... protein bands on the immunoblots was quantified by densitometry scanning and the intensity of each band was determined using the NIH Image software The enzymatic activity of the P-DN42 and mutant ... of the domain structure and the position of the cysteine residues in the cloned GalNAc-transferases Arrows indicate cysteine residues and the numbers indicate residues mutated in this study The ... ends of the putative b-strands, lining the face of the predicted active site cleft [15] These proposed structural features of the GT1 motif in GalNAc-T1 are similar to the UBD, raising the possibility...
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Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

Báo cáo khoa học

... a similar position as the lowest faint band observed for wild-type Kv3.1 and the lower faint band of the single mutants, and furthermore, the immunoband did not shift in the presence of TM To ... to the lower two immunobands of wild-type Kv3.1 In both cases, the upper band was darker than the lower band Additionally, the upper band was not visible in the presence of TM A single immunoband ... a predominant immunoband at about 87 kDa and two lower faint bands The lowest band migrated to about 77 kDa, and the middle band was at about 81 kDa Only the two lowest bands were detected when...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt

Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt

Báo cáo khoa học

... determine whether the remarkable change in the amino acid requirement at position was specific for Ser or not, the Ala at position in MG3X6 A-TNF was changed to other amino acids, and their susceptibility ... N-myristoylation, and the amino acid requirements at this position were significantly affected by the amino acid at position [25] Therefore, we next determined the effect of the amino acid at position on the ... incorporated into the upper of the two bands detected with [3H]Leu (lane 6) As there is no Met residue in the mature domain of TNF, this result indicates that the upper band corresponds to the protein...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx

Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx

Báo cáo khoa học

... determined by their site of expression, their selective target sequence recognition, their selective abilities to interact with other transcription factor coactivators, and their selective modification ... initiation requires at least one of the two sites It appears that the )70 Sp1 motif is required whether initiation is directed by either of the )10 or +90 Ets site Ó FEBS 2004 Regulation of the presenilin ... activates the PS1 ERM transactvates PS1 and does not require the )10 Ets element We have begun to identify the Ets factors controlling the expression of PS1 using the yeast one-hybrid selection and the...
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Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx

Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx

Báo cáo khoa học

... PALQ) and P19A-mBS (panel P19A), in comparison with the same region of the parent mBS (panel mBS) The new signal at 8.40–1.40 p.p.m., which appears in the spectra of P19A-mBS and Fig Far- UV spectra ... dithiothreitol and gel filtered The fractions containing the reduced dimer were pooled and air oxidized The amount of the MxM form in the isolated sample at this stage was 85% Aliquots were then separately ... missing, and are replaced by a set of new signals tentatively attributed to Gln28 (data not shown) Apart from these expected differences, the high similarity among the spectra of the variants and the...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf

Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf

Báo cáo khoa học

... properties of the enzyme [19] To extend the information about the role of the noncatalytic SBD regions and their effect on the C-terminal CD, we further explored the amino acids of the N-terminal ... PPase is the first committed step in starch biosynthesis, SS catalyzes the elongation of a-1,4-glucans by the transfer of the glucosyl moiety from the sugarnucleotide to the nonreducing end of the ... D(316–344) in the D2 domain and D(495–535) in the D3 domain, are involved in the interaction with CD, and that this interaction enhances the catalytic activity of the enzyme Our results show that the interaction...
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