the domain of cooking tasks

... TotalTime. The number of inter- vals present during the total time period is calculated by dividing the total time period by the sum of the length of one incident of the action and the length of a ... duration of each of these actions. 65 Defining the Semantics of Verbal Modifiers in the Domain of Cooking Tasks Robin F. Karlin Department of Computer and Information Science University of Pennsylvania ... is part of the representation of the primitive actions. The number of stirring events which fit in the total time period is calculated. The output consists of repetitions of pairs of the following...

Ngày tải lên: 21/02/2014, 20:20

Ngày tải lên: 05/05/2013, 21:33

Ngày tải lên: 05/03/2014, 23:20

Ngày tải lên: 20/06/2014, 21:20

Ngày tải lên: 22/06/2014, 06:20

Ngày tải lên: 22/06/2014, 19:20

Ngày tải lên: 23/06/2014, 00:20

... not yet been addressed. The first question is whether the expression level for RIZ1 varies over the progression of cancer; and the second question is whether the PR domain alone possesses ... described below; and the cell death rate was exam- ined by the trypan blue method after 24 and 48 hr of transfections, respectively. Western blot of PR domain The expression of the PR domain was examined ... suggested that the PR domain alone pos- sesses anticancer activity. However, it is still unclear whether the anticancer activity of the PR domain is due to its methyltransferase activity or the interaction...

Ngày tải lên: 25/10/2012, 11:15

Ngày tải lên: 07/09/2013, 13:37

... independently of Pbx, and does not possess the autoinhibitory function of the Hth domain. In summary, our data suggest that one function of the conserved Hth domain is to inhibit the activity of the transcriptional ... significantly derepressed by deletion of the Hth domain, suggesting that the inhibitory effect of this domain is not specific to the Meis2d AD. Comparison of the relative expression levels of these GBD fusion proteins ... region 2 (hr2) of the Hth domain, derepressed it to a similar degree as the full Hth deletion (Fig. 4A). To test whether the inhibitory activity of the Hth domain was specific to the Meis2 AD,...

Ngày tải lên: 16/02/2014, 15:20

... tertiary fold in the cytoplasmic region of VpU, as indicated by a small number of observed long-range NOEs [24]. Another important aspect is the topology of the cytoplasmic domain of VpU on a membrane. ... most studied activities of VpU is the induc- tion of CD4 degradation in the endoplasmatic reticu- lum of infected CD4 + cells. Only the cytoplasmic domain of VpU, together with any membrane anchor, is ... undetectable in the presence of Mn 2+ . The sol- vent-exposed C-terminus of VpUcyt probably pointed away from the surface of the micelle. The three amide cross-peaks originating from the hydrophobic...

Ngày tải lên: 18/02/2014, 06:20

... fix the SEA domain in a loca- tion on the opposite side of the proteinase domain from the active site cleft. It seems very unlikely, there- fore, that the SEA domain would directly influence the binding ... cystein-rich (SRCR) domain, which was rigidly bound to the back of the proteinase domain in the crevice between the C-terminal helix, the 204-loop and the 126-loop [7]. The C-terminus of hepsin is elongated ... Lys224– Tyr228 (the back of the pocket) and the disulfide bridge Cys191–Cys220 (the front of the pocket) (Fig. 4A). The backbones of these segments form a deep hydrophobic pocket with the negatively...

Ngày tải lên: 19/02/2014, 00:20

... domains differ with respect to the lability of the zinc ions and that, despite the increased thermodynamic stability of the a domain with Zn 2+ over the b domain, the Zn 2+ sites in the a domain were shown ... sites within the two domains. This was followed by coordi- nation of the fourth and fifth metal ions to the a domain for completion of the metallation of this domain prior to filling of the b domain. ... example of the complexity introduced by the presence of the b domain in efforts to elucidate the potentially cooper- ative nature of the metal-binding reaction within each of the domains. Thus, the...

Ngày tải lên: 19/02/2014, 00:20

... within a cutoff distance (r c ¼ 6.0 A ˚ ). The dynamics of the resulting network are then defined by the Ni · Nj Kir- chhoff connectivity matrix of interresidue contacts (G) where the off diagonal ... (nucleophilicity) for the in silico creation of the various glycoconjugates was determined by calculating the electrostatic potentials (EP) for the N e of the lysine residues of a-CT at pH 9 were the chemical ... some of these phenomena may be interconnected within the catalytic mechanism of the enzyme. Structural insights into the mechanochemical nature of a-CT catalysis A more detailed analysis of the...

Ngày tải lên: 19/02/2014, 05:20

... binding of the polyadenylated rpsO RNA. We have shown that the presence of the remainder of the acidic tail results in the thermodynamic stabilization of Hfq by 1.8 kcalÆmol )1 . A comparison of the ... destabilize the network of hydrogen bonds. The crystal structure of the first 71 amino acids of E. coli Hfq indicates that the C-terminal domain is probably located at the top of the ring, because the ... another. The difference between the Hfqs results from the insertion of a fragment of variable length between the first 67 amino acids and the end of the acidic C-terminal domain. Determining the...

Ngày tải lên: 19/02/2014, 12:20

... to the Grb4 SH2 domain. The 1 H- 15 N HSQC spectrum of the Grb4 SH2 domain (Fig. 2A) Fig. 2. Characterization of the Grb4 SH2 by heteronuclear NMR. (A) The 1 H- 15 N HSQC spectrum of the SH2 domain ... step of the expressed SH2 domain of Grb4. Lane 1: the soluble part of the cell lysate; Lane 2: the insoluble part of the cell lysate; Lane 3: the Ni-nitiloacetic acid agarose beads bound with the ... conditions. The quality of the expressed SH2 domain is improved at each step of the purification procedure (Fig. 1B). A synthetic DNA fragment encoding the PDZ domain of PDZ-RGS3 was prepared in the same...

Ngày tải lên: 19/02/2014, 12:20

... later than in the case of the histone gene cluster [10]. This difference in the tim- ing of somatic pairing perhaps reflects the difference between the complexities of the regulation of the two systems: ... approach their proper target promoter in the viscous environment of the nucleus. Somatic pairing of chromosomes and the regulation of Abd-B A peculiarity of Drosophila is the fact that the homo- logous ... required for the formation of the complex looping structure in the case of BX-C, while a shorter time is sufficient for the establishment of the much simpler regulatory interactions of the his- tone...

Ngày tải lên: 20/02/2014, 01:20

... cluster in these two regions. Know- ledge of the structure of a larger portion of MeCP2 including the transcriptional repression domain would resolve these speculations and clarify the role of the helical ... [18]. The structure of the helical coil a 2 /a 3 allows us to interpret the consequences of the six mutations. As P153(152) and G162(161) are buried in the protein core, replacement of each of these ... one-turn helix on the other face. It is thought that the two inner strands of the b-sheet lie within the major groove of the DNA and that a hydrophobic pocket formed by the side chains of Y123 and...

Ngày tải lên: 21/02/2014, 00:20

... PrP. Production of deletion mutants of PrP On the basis of the results with antibodies, a series of PrP mutants were made to assess whether deletions of certain domains of the protein decrease the SOD-like ... for the SOD activity. We determined that the active site consists of two domains. The first includes the Cu-binding domain and the second includes the conserved hydrophobic domain in the middle of ... activity of the protein. The domains deleted were based on the epitopes of the antibodies that had a clear effect on SOD-like activity of PrP. The mutants used in the study include the complete N-...

Ngày tải lên: 21/02/2014, 00:20

... possesses the same characteristics of the interior of the protein matrix. The script a is the peak–peak distance between the maximum at %287 nm and the minimum at %283 nm, and the script b is the peak–peak ... alignment of the N-terminal domain of PsbQ from spinach and Chlamydomonas reinhardtii, where the charged residues are indicated. (C) View of the 3D model for PsbQ, where the non-modelled N-terminal domain ... Cl – [32] and (c) to tune up the magnetic properties of the Mn cluster [33]. It will be very useful to address the analysis of the complex with information about the structure of the individual complexes....

Ngày tải lên: 21/02/2014, 00:20