... for routine testing of large numbers of samples Therefore, the inspection of food for the presence of Salmonella has become routine all over the world (Malorny, Paccassoni et al 2004) The rapid, ... characterized by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles The higher the starting copy ... product at the end of the PCR cycle Diagnostic real-time PCR for the specific detection of Salmonella in foods ¡is increasingly being used as a rapid and reliable tool for the control of contaminated
Ngày tải lên: 31/07/2024, 17:15
... in the case where half of the population was made up of F2 individuals. The other half was equally divided between PI, P2 and Fl individuals. Finally, considering these ... comes from the detection of a mixture of subdistribu- tions within the global distribution of either F2 and/or backcrosses. In principle, the test statistic used (the likelihood ... The detection power for additive genes was low and nearly independent of the population size and structure. In contrast, in the case of a dominant gene, the power increased
Ngày tải lên: 09/08/2014, 18:21
báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc
... (Fig- ure 4). On the contr ary in SBEIIa-D (D462N) the mutation caused the loss of two of the seven amino acids involved in the binding and catalytic activity in normal SBEIIa protein, respectively ... quency of the wheat population used in the present work did not require deep pooling to increase the throughput of the screening. Our finding of a mutation density of 1 SNP for each 40 kb is in agreeme ... in the region of the N-term- inal domain of t he glycogen branching enzyme family, reported to be essential for the size of the glucan chains transferred and also for the catalytic activity of
Ngày tải lên: 11/08/2014, 11:21
Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... role in these lesions. The purpose of this study was to compare the efficacy of HC-II assay and PCR for the detection of specific HPV type (HPV 16 E6) in OSMF and OSCC cases as well as find out the ... of the intensity of HPV infection. This ratio of any specimen represents empirically a relative measure of the viral load in it. Cut off val ue (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of ... suspicious lesions of theoralcavitybyasoftbrushprovidedinthekit.Gen- tle rolling strokes were made over the affected area as per the manufacturer’ s instructions and samples were collected in the sample
Ngày tải lên: 12/08/2014, 01:22
Báo cáo sinh học: "A microsatellite-based analysis for the detection of selection on BTA1 and BTA20 in northern Eurasian cattle (Bos taurus) populations" doc
... and significance of LD in the cattle populations using the Haploview 4.0 program. Numbers in the blocks indicate the percentage of the LD metric D’ values > 0.3; shadings indicate Fisher’s ... nRθ-test is largely independent of the demographical past, the additional power of linked loci is balanced by the cost of an increasing dependence of the demographic past due to the fact that LD ... heterozygotic status at the POLL locus in 19 phenotypically polled cattle of the three Finnish native populations, on the basis of their offspring/parent phenotypes . In addition, there were 19 animals
Ngày tải lên: 14/08/2014, 13:21
Báo cáo y học: "Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies" docx
... Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, FI-20521, Finland †Department of Mathematics, University of Turku, Turku, FI-20014, Finland Correspondence: Laura L ... Laajala 2009 10, Issue 7, Article R77 Method Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies Essi Laajala*, Tero Aittokallio*†, Riitta Lahesmaa* ... under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the
Ngày tải lên: 14/08/2014, 21:20
On the detection of seasonal variation in the onset of disease
... regression. This includes the presentation of childhood cancers in the United States of American (USA), breast cancer in Singapore, the cases of methicillin-resistant staphylococcus aureus in Spain, and ... each of these studies had identified and reported a date of peak incidence 4 One problem in the investigation of seasonality is that of missing values or incomplete years of observation ... of onset was observed in either Singapore or the 11 distinct locations in the USA. In contrast, a strong peak (early January) was found in Sweden but the 95% confidence interval (November 17
Ngày tải lên: 16/09/2015, 17:11
Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate
... 4.4 The density of each type of bacteria in Ash – PRQherbicide mixture 32 Table 4.5 The density of each type of bacteria in Ash - GPTherbicide mixture 32 Table 4.6 The density of bacteria in ... one of the amplification primers may be used in Sanger sequencing, isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into ... concentration and their growth within the contaminated environment in laboratory This research brought many significances in scientific learning and research areas In terms of this aspect, doing the proposed
Ngày tải lên: 28/04/2016, 13:40
Prevalence and psychometric screening for the detection of major depressive disorder and post-traumatic stress disorder in adults injured in a motor vehicle crash who are engaged in
... than suggest the likelihood of the disorder is high, with larger the number, the more convincing that the detection of the disorder is correct LRs between and suggest the likelihood of the disorder ... pain and suffering Claimants must have reported the accident and injuries within 48 h of the road crash, and lodge the CTP claim within months from the date of the crash This study is part of ... all items in the scale have been included in the cut-off calculation None of the above papers have reported sensitivities or specificities related to the cut-off scores employed The findings from
Ngày tải lên: 10/01/2020, 14:22
Effectiveness of PIVKA-II in the detection of hepatocellular carcinoma based on realworld clinical data
... especially with the development of next-generation sequencing, immune therapy and targeted drugs [4–6] However, things are different in the area of hepatocellular carcinoma (HCC) Due to the inadequate ... written into the guidelines of the Japan Society of Hepatology (JSH) [16, 17] © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International ... Key Laboratory of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China Full list of author information is available at the end of the article Africa
Ngày tải lên: 06/08/2020, 03:54
Retrospective and comparative analysis of 99mTc-Sestamibi breast specific gamma imaging versus mammography, ultrasound, and magnetic resonance imaging for the detection of breast cancer in
... false-negative findings of BSGI We had 20 cases of false-positive BSGI findings The pathology of false-positive BSGI lesions is shown in Table We also analyzed the false-negative findings according to the ... is the leading type of new cancer cases and the second leading cause of cancer related deaths in females worldwide [1] During the past 30 years, the incidence and mortality of breast cancer in ... imaging tests (BSGI, US, Fig a ROC analysis for determining cut-off value of TNR in detection of breast cancer b TNR of BSGI distribution in breast malignant and benign disease c Sensitivity of
Ngày tải lên: 21/09/2020, 01:57
Tailored media for the detection of E. coli and coliform in the water sample
... villages They are the main sources of water So in most of the villages the people have to go daily almost a kilometer for the search of drinking water as well as for cleaning purposes They don’t ... health than the people of developing countries However, the people of developing countries including Nepal should fight against intestinal diseases Water, which may appear pure to the nake eye, ... a variety of processes depending upon the source and nature of the water These processes include clarification, sedimentation, filtration and disinfections The overall main aim of these procedures
Ngày tải lên: 27/09/2020, 15:24
Analytic performance studies and clinical reproducibility of a real-time PCR assay for the detection of epidermal growth factor receptor gene mutations in formalin-fixed paraffin-e
... HJL oversaw the study design and conduct of the external reproducibility study and was involved in drafting of the manuscript RS was involved in the study design and conduct of the clinical reproducibility ... of the use of next generation sequencing in routine clinical diagnostics, for the accurate selection of patient therapy, method of testing for EGFR mutations should be well validated both clinically ... running all analytical performance and verification testing FS was involved in drafting the manuscript and interpretation of the data WW, LU, SS were involved in study design and acquisition of the
Ngày tải lên: 05/11/2020, 06:20
molecular assays for the detection of prostate tumor derived nucleic acids in peripheral blood
... prognostic indicator of aggressive prostate cancer Enumeration of prostate CTCs alone provides minimal information of the metastatic nature of these cells, although the presence of CTCs in blood ... by increasing the analytical sensitivity of these assays For example, the PCA3 assay used in the present study was developed originally for urine specimens [18] A more sensitive version of the ... following definitive biopsy Furthermore, tests are needed to detect disease recurrence following radiation and/or surgical intervention, especially considering the increasing rate of targeted therapies
Ngày tải lên: 02/11/2022, 14:24
design of electric field assisted surface plasmon resonance system for the detection of heavy metal ions in water
... refractive index can then be considered to be a constant value of 1.881 that is calculated using a linear fitting of the plot in Fig The final term in Eqn.(8), which is the change of the signal ... “SPR dip” in the figure The location of the SPR dip shifts with the changes on the sensor surface as demonstrated in a line plot of reflectivity in Fig.5(c) The plot in Fig 5(c) contains the SPR ... across the channel The intensity was found to increase gradually during the ion deposition till it reaches a saturation point The slope of the curve represents the linear rate of change in the signal
Ngày tải lên: 24/11/2022, 17:39
Validation of an Automatic Video Monitoring System for the Detection of Instrumental Activities of Daily Living in Dementia Patients
... corner) The activity modeling was performed with the support of domain experts The models were mostly made taking into account one or more of the following constraints: the presence of the person in ... Over the last few years, the use of new technologies for the support of elderly people and in particular dementia patients received increasing interest We investigated the use of a video monitoring ... specific zone, their posture, and their proximity to the object of daily living (when static, e.g., the telephone) These constraints were defined as primitive state models The combination of these models,
Ngày tải lên: 10/04/2023, 20:57
Circuit theory of finance and the role of incentives in financial sector reform
... placing money with investment financial institutions in the capital market, and hold the remaining share in noninterest bearing, short-term bank deposits (eq 7) The share of savings going to the ... focus of the theory, however, leaves the essential microeconomic aspects of finance out of the picture In particular, although recognizing time as a fundamental determinant of the circuit, the theory ... understanding More progress can be gained by further exploring the implications of the time dimension in the economic process This will be one of the undertakings of this paper The paper analyzes the
Ngày tải lên: 24/10/2012, 09:33
Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... measured by the MCG device The resulting mathematically integrated patterns of the abnormal indexes are then compared for their degree of abnormality to the abnormal index patterns in the reference ... http://www.medsci.org Int J Med Sci 2009, analyses included in the database were performed using the same “made in USA” equipment as in the included trials and were analyzed using the same software and ... quality of the tracing was visually rechecked and graded as “good,” “marginal,” or “poor” A poor tracing was defined by one of the following: • five or more 5.12-second segments of ECG data containing
Ngày tải lên: 03/11/2012, 10:58
The Art of Building in the Classical World
... envisioningof theoria (an envoy’s seeing of truth) according to geometric and optical mod-els that first came into being in the graphic planning of the architectural typeof the theatron (the place ... opening toreceive the flow of the Tao in order to bring forth the universe.39As in the Yang, for Plato as well there is the repeated metaphor of a cre-ative outflow of light Yet the Idea of the ... into the eyes, which takes place in ? ?the eye of the soul” (t¼ tvyucv Àmma, 533d) as well .The metaphor of sex therefore heightens the reader’s awareness of the rience of seeing, amplifying the
Ngày tải lên: 15/11/2012, 10:32
The trend of globalization in every field
... overview of the study in which the rationale for the research, the research problem, the purposes, the research questions, the scope of the study, the research method, the significance of the study, ... means the teaching of pronunciation depends largely on the teacher’s turning on and rewinding a cassette player (or any other instrument), and the main activities in the class are listening and ... English in the future. Therefore, their English pronunciation must go far beyond the “accepted level”, as they are going to teach English for the coming generations of the country. After finishing
Ngày tải lên: 05/04/2013, 21:13