the curious paradox of heat shock proteins as tumor specific antigens
Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... (2009) ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 ... among the regulation of nucleolin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative stress and Hsp70 overexpression More importantly, for the first ... nucleus and cytoplasm of the cell, as well as at the cell surface [10] Nucleolin ⁄ C23 regulates many aspects of DNA and RNA metabolism, such as RNA polymerase I-mediated transcription, the folding,...
- 11
- 614
- 0
Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis ... decreased the size of the inclusions but the effect was less intense (average size of 10 lm reduced to about 4–5 lm) In contrast, the size of the inclusions was not significantly altered by the ... was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the percentage of cells with FL2-H fluorescence greater than · 101 in the samples to that...
- 18
- 721
- 0
Báo cáo khoa học: The influence of cold shock proteins on transcription and translation studied in cell-free model systems pdf
... Instead of these, mRNA was used as template for the translation With this system, the effect of the CSPs on the translational level was studied In the absence of CSP the expression rate was approximately ... or of masking of regulatory sequences The described translation inhibition of a non-cold shock mRNA can be combined with the results of [3] They found an increase in the translation of cold shock ... and the plasmid pK7CAT The kit was used for the generation of template RNA for in vitro transcription assays and for analysis of the inuence of CSPs at the transcriptory level In the latter case...
- 12
- 487
- 0
Local induction of heat shock proteins using magnetic fluid hyperthermia for ocular neuroprotection in glaucoma
... tuning the AC heating characteristics of SPNPs to improve the efficiency of HSPs 72 induction c Identification of the induction of HSPs 72 d Investigation of the correlation between the change of ... hyperthermia Experimental set-up for AC heat measurements of ferrofluids Formation of colloidal suspension Schematic diagram of the property of SPNPs fluid Kinds of magnetism Hysteresis loops of ... Fe3O4@PEG ferrofluid The specific loss power (SLP) of all the ferrofluids calculated based on the AC heating characteristics obtained in Fig 7-8 Studies of in-vitro biocompatibility of uncoated...
- 191
- 205
- 0
Immunomodulatory roles of heat shock proteins
... recombinant heat shock proteins in the presence or absence of polymyxin B for another 16 h The NF-κB-Luciferase expression was determined using the Dual Luciferase Reporter Assay (DLR Assay, Promega) ... cross-presentation of ovalbumin (OVA) by DC2.4 to the CD8 T cell hybridoma B3Z, as determined by the increased IL-2 production In the presence of 31 the heat shock proteins, B3Z (specific for the SIINFEKL ... expression has led to dramatic decrease of the ability of DC to cross-present peptides [Binder and Srivastava, 2004] However, other receptors for the uptake of the HSP-peptide complexes such as CD40...
- 152
- 547
- 0
Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... 2005 FEBS Small heat shock proteins and disease as does the R116C mutation However, in contrast to R116C aA-crystallin, the R49C variant localizes to the cell nucleus and the cytoplasm, superficially ... clearer, as is the molecular basis of oligomerization The implications of oligomer assembly and disassembly as chaperoning prerequisites are under study, sHSP substrates have been identified, and the ... function of the small heat shock protein 16.5 from Methanococcus jannaschii J Biol Chem 277, 38468– 38475 21 Golenhofen N, Perng MD, Quinlan RA & Drenckhahn D (2004) Comparison of the small heat shock...
- 15
- 573
- 0
Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt
... Brg1 during the G1 phase of the cell cycle and regulates the expression of heat shock proteins J Cell Biochem 98, 1528– 1542 26 Kroeger PE, Sarge KD & Morimoto RI (1993) Mouse heat shock transcription ... and heat- shocked at 39 °C for the indicated times Total RNA prepared from the cells was subjected to RT-PCR analysis The genes targeted by yHSF are classified according to the structure of their ... hHSF4–VP16, without changing the magnitude of the heat shock response (cp3) The mutational analysis of the CRYGA and CRYGC HSEs suggests that the nucleotide sequences and configuration of nGAAn-like units...
- 13
- 507
- 0
Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx
... effectiveness of the chaperone Using this assay we tested the relative activity of the two wheat proteins in suppressing aggregation of MDH As shown in Fig 5, both wheat Fig Wheat sHsps suppress heat- induced ... was performed as above except the mobile phase was 200 mM Na/Pi, 100 mM NaCl, pH 7.3 Firefly luciferase reactivation assays Luc was heat- inactivated at 42 °C in the presence of sHsp as described ... TaHsp16.9C-I, suggesting class II proteins have a distinct mode of oligomer assembly as compared to the class I proteins Using malate dehydrogenase (MDH) as a substrate, TaHsp16.9C-I was shown to be a...
- 11
- 386
- 0
Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... role of HSP70 in the inhibition of the release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role of the ATP-binding domain of HSP70 in the prevention of the release of ... (HSP70WT) in other functional assays, including the inhibition of the activation of caspases-9 and -3 (Fig 6A) after exposure to H2O2 for h, as well as the inhibition of H2O2induced apoptosis as assessed ... were assayed for protease activity of caspases-9 or -3 using caspase fluorescent assay kits, and apoptotic cells were identified by elevated activation of caspases-9 and -3 The experiment was repeated...
- 10
- 726
- 0
Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc
... solution of cesium iodide, and processed using MASSLYNX software (Micromass UK Ltd) The peak series were identified manually A mass was generated based on an algorithm built into the MASSLYNX software, ... 25 °C (B) Thermal aggregation of citrate synthase (CS) at 43 °C in the presence of different concentrations of HspB or HspB(G116A) was recorded at 360 nm The CS assay was performed in the absence ... lines assemblies A transfer of CS and HspH into the void volume, at the expense of the other fractions, was confirmed by SDS-PAGE (Fig 7C) Neither HspH nor CS shifted into the void volume when they...
- 10
- 289
- 0
Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human aB-crystallin docx
... units against time in the presence of increasing ratios of MTB HSP 16.3 to CS With the increase of MTB HSP 16.3, there was an increase of protection against thermal aggregation of CS [d, CS alone; ... of stirring The mixture was removed from the ice bath and 200 lL of mgÆmL)1 DNAse was added with stirring for 30 The sample was then placed in a 50-mL tube and centrifuged at 18 000 g for h The ... measure the aggregation of CS in arbitrary units vs the ratios of MTB HSP 16.3/CS and human aB-crystallin/CS With increased ratios of the molecular chaperone protein to CS, there was increased...
- 8
- 310
- 0
Báo cáo Y học: Determination of the consensus binding sequence for the purified embryonic heat shock factor 2 potx
... and the DNA was purified on a 12% acrylamide gel About 50–100 ng of DNA was used for the next cycle of the SELEX procedure Cloning of the products of selection DNA amplified from the last cycle of ... [29] The strategy consists in measuring the affinity of the factors for a series of oligonucleotides containing an increasing number of the binding motif Immediately following the addition of the ... 9) The sequence of JUL, as well as the sequence of Shvalspz, are very close to the consensus binding sequence determined by the SELEX assay (they both contain the 8-mer core), which explains the...
- 11
- 421
- 0
Báo cáo Y học: A critical motif for oligomerization and chaperone activity of bacterial a-heat shock proteins pot
... function of the small heat shock proteins in plants J Exp Bot 47, 325–338 Arrigo, A.-P & Landry, J (1994) Expression and Function of the Low-molecular-weight Heat Shock Proteins In The Biology of Heat ... and their apparent molecular mass of 350–480 kDa was consistent with oligomers of 20–30 subunits (Fig 3A,E) Thus, the integrity of the extreme N-terminus is not relevant for the assembly of functional ... in a total volume of 1.0 mL of 50 mM phosphate buffer, pH 6.9 CS aggregation was measured by the increase of absorbance at 360 nm in the absence (r) and in the presence of a-Hsp proteins at a final...
- 9
- 467
- 0
Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 1
... ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY NATIONAL UNIVERSITY OF SINGAPORE 2011 INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT ... REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY (B SCI (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY YONG LOO LIN SCHOOL OF MEDICINE NATIONAL ... DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2011 ...
- 3
- 299
- 0
Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 2
... et al., 2005) Each of these modifications has their role in the regulation of transcription In this project we investigated the role of ubiquitin in the regulation of the heat shock gene HSP70B’ ... The Role of Ubiquitin in Transcriptional Regulation 40 1.5.4 The Proteolytic Role of the Proteosome in the Regulation of Transcription 42 1.5.4 The Non-Proteolytic Role of the Proteosome in the ... Determining the Existence of Ubiquitinated Species of Hsf1 137 3.13 The Stability of Hsf1 141 3.14 The Presence of Hsf1 at the HSP70B’ Promoter 149 vi Chapter 4: Discussion 158 4.1 Key Findings of the...
- 16
- 266
- 0
Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 3
... in the template strand to place the coding DNA in the “catalytic cleft” of the RNA polymerase II complex After the synthesis of the first 30 or so bases, RNA polymerase II is thought to release ... the destruction of proteins answered the key criticisms leveled against the Lysosome hypothesis The next question that needed to be answered was what else was there is the elute fraction of the ... was commonly thought of at the time as the typical form of a protease (Ciechanover et al., 1978) Further purification of the flow through revealed an 8.5kDa, protein that was necessary for the...
- 198
- 509
- 0
Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt
... [4] The helical repeat of the DNA (10.5 bp), the length of the unit (5 bp) and the inverted nature of the repeats, positions all of the GAA units on the same side of the double helix, with the ... structure of the yeast Kluyveromyces HSF DBD complexed with the sequence ggTTCtaGAAcc (containing one set of the nGAAn inverted repeat) has been determined [15] In the complex, the third helix of the ... co-crystals, the protein–protein interface consists of the helix amino-terminus, the turn and the wing [15] Unlike other winged helix-turn-helix proteins, the wing of the HSF does not appear to contact the...
- 10
- 565
- 0
Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
... migration of the spindle during cytokinesis In this case, the depletion of Hsp90a in the absence of HSF1 affects the mitogen-activated protein kinase pathway This study describes the role of HSF1 as ... the loss of other actors in the migration process, such as members of the doublecortin family Dcx and Dclk [146–148] Conclusions The emerging landscape of the role of HSFs in development is the ... binding was shown to be closely associated with reduced methylation of the histone H3K9, irrespective of the relative location of the HSF4-binding regions and of the transcriptional status of genes...
- 23
- 796
- 0
Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... measurement of the PGC, the 10 Time (s) 15 20 Fig FCS and FRAP analyses of OlvasGFP in the PGC FCS was used to measure the movement of OlvasGFP into the cytosol out of the nuage region of the PGC Representative ... of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the ... measured outside of the nuage in the cytoplasm of the migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement of the...
- 9
- 655
- 0
Báo cáo khoa học: Human telomeric G-quadruplex: The current status of telomeric G-quadruplexes as therapeutic targets in human cancer pdf
... displacement of bound proteins from the singlestranded overhang, chiefly hPOT1, as well as possible uncapping of telomerase from the ends There are likely to be multiple mechanisms involved, some of which ... catalytic subunit of telomerase (hTERT in humans) has reverse transcriptase enzymatic activity and synthesizes TTAGGG repeats on to the end of the 3¢ single-stranded overhang Inhibition of hTERT by ... little evidence of concomitant telomere shortening [11,18,32] This has subsequently been shown to be characteristic of the G-quadruplex ligand class as a whole, and the observations of on-target...
- 8
- 446
- 1
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose