the application of btem to ir and raman spectroscopic data

... 5.2.4 In-situ Spectroscopic Measurements 117 vii 5.2.5 Data Analysis: The Application of BTEM to IR and Raman Spectroscopic Data 120 5.3 Results: The Experimental Far -IR and Far -Raman Spectra ... determination of the number of observable species present and their pure component spectra, (2) determination of all the moles of all species, the number of reactions, and the reaction stoichiometries, ... goals of this thesis are: (1a) the development of new and broadly-applicable algorithm, namely Chiral -BTEM (1b) the application of this new algorithm to types of polarized spectroscopies and to...

Ngày tải lên: 12/09/2015, 08:20

... places the top n records at the top of the view Next, get the Freight value for the nth record and set the DataView filter to contain only rows with a Freight value greater than or equal to that ... as is the case with Freight In this case, iterate over the records following the nth record and add criteria to a copy of the data view filter to exclude them from the view Use either the primary ... combination of columns to identify the row to be excluded in each case Apply the new filter to the view If the view is ordered on the primary key or unique columns in addition to the top n columns,...

Ngày tải lên: 14/12/2013, 18:16

... application of this method by referring to the actor and the historian as the historian of the future” and the historian of the past,” respectively.20 Regardless of the purpose for which it is used, therefore, ... interests The ideology is the smoke screen for their loot, the fictitious clothes spun by the intellectuals to hide the naked plunder of the Emperor The task of the historian, then, is to penetrate to ... with the choice of ends and of the means resorted to for the attainment of these ends, and with the valuation of the outcome of actions performed.21 Furthermore, whether directed toward planning...

Ngày tải lên: 17/01/2014, 02:20

... = 150 N Problem 2-17 Determine the magnitude and direction of the resultant force F R Express the result in terms of the magnitudes of the components F and F and the angle φ Solution: F R = F ... Problem 2-20 The plate is subjected to the forces acting on members A and B as shown Determine the magnitude of the resultant of these forces and its direction measured clockwise from the positive ... Problem 2-41 Determine the magnitude and direction of the resultant FR = F + F2 + F of the three forces by summing the rectangular or x, y components of the forces to obtain the resultant force...

Ngày tải lên: 17/02/2014, 14:20

... requires a cytosolic chaperone-mediated association of precursor protein with peripheral translocase of outer membrane (TOM) receptors (TOM20, TOM22 and TOM70), which enables the translocation of ... targeted to mitochondria in transiently transfected hepatoma cells [22] The nature and specificity of the endoprotease and the site of proteolytic cleavage remain unknown The same investigators were ... experiment, we determined the concentration of digitonin required to release mitochondria-associated CYP2E1 into the soluble fraction, and compare it with the amounts needed to release the outer membrane-specific...

Ngày tải lên: 18/02/2014, 16:20

... as the ligand-macromolecule reaction occured A binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macromolecule ratio From the ... heavily in the presence of Cu2+ at pH and to a lower extent at pH Therefore, no comparison of the stability of the tertiary structure to the effect of Cu2+ could be made Influence of copper on ... [12,14] Therefore, the differences in the far UV CD must be due to the contribution of tyrosines to this region of the spectrum [28] Figure 4B shows the near UV CD spectra of stefin A, stefin B, and the...

Ngày tải lên: 19/02/2014, 06:20

... study and RT-PCR analysis of transcripts (A) Schematic representation of the pAO1 gene and ORF cluster flanked by Tn554 and DTn The cluster consists of the pmfR gene, encoding the regulator of the ... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities of the proteins Our results demonstrate the presence of two pathways of ... members of the polyamine oxidases, the FAD cofactor was noncovalently bound to the apoprotein and the C-terminal fingerprint sequence SGGCY of monoamine oxidases, with C being the cysteine residue to...

Ngày tải lên: 19/02/2014, 07:20

... indirect effect on the binding environment of fosfomycin The direct influence comes from two hydrogen bonds formed by the nitrogen of the N-acetyl group and the 3¢-oxygen to the phosphonate and ... ligand binding The loop region of the upper domain moves towards the active site of the enzyme, thus shielding the substrates (and ligands) from bulk solvent A detailed thermodynamic study of ... independent of the thermodynamic and kinetic properties of the binding process and is solely based on the detectability of the peptides of interest We have used this method to show that binding of fosfomycin...

Ngày tải lên: 19/02/2014, 13:20

... important for the GABA transport activity 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal structures of the N-glycans of GAT1, ... (Fig 2B,C), the large band is assigned to proteins with N-glycans of mature complex type and the small band to the proteins with N-glycans of mannose-rich type All bands of the mutants had reduced ... GAT1 to the plasma membrane In order to study the influence of N-glycosylation on plasma membrane trafficking of GAT1, the distribution of wild type and mutants on the cell surface and in the cell...

Ngày tải lên: 19/02/2014, 17:20

... acid [40,41] They are prime target of ROS due to their location near to the site of ROS production [42] and could be the source of carbonyl adducts Other recent reports have shown that there is intracellular ... between the relative rates of glycoxidation/oxidation and proteolysis in the mitochondrial matrix Although the respiratory function of mitochondria is not compromised in spite of the extent of altered ... ng atoms of oxygen consumed per minute and per mg protein The rate of oxygen consumption in state and in state 4, respiratory control ratio (RCR) of oxygen consumptions in states and and the...

Ngày tải lên: 20/02/2014, 11:20

... initiated through productive binding to the substrate via two elements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer In the case of short unstructured peptides or unfolded ... interaction between ClpS and the N-domain of RR ⁄ AA, and this result suggests that neither the local nor the overall structure of the RR ⁄ AA mutant was compromised Mutation of the conserved arginine ... wished to compare the ability of the RR ⁄ AA mutant to bind to the various substrates tested To this, we constructed a ClpA variant in which the glutamic acid residue within the Walker B motif of...

Ngày tải lên: 07/03/2014, 05:20

... mol) The results are mean ± SD, n ¼ 1–4 The degree of permeabilization is expressed as the percentage of the maximal value obtained at the end of the assay by the addition of mM Triton X-100 The ... Anderluh et al Stefin B and cellular toxicity A Insertion in monolayers B The ability of stefins and their aggregates to insert at the air–water interface, i.e in the absence of lipids, was determined ... pH 3.3 (up to 40% loss of viable cells) Therefore, the MTS test appears suitable for discriminating the cytotoxic effect of the stefin prefibrillar forms In order to determine whether the prefibrillar...

Ngày tải lên: 07/03/2014, 17:20

... reported for other members of the neurohypophysial hormone receptor family; for example, the distal N-terminus is required for agonist binding to the OTR [11,12] and also to the vasotocin receptor [13], ... NEN) [18] as the tracer ligand Binding data were analyzed by nonlinear regression to fit theoretical Langmuir binding isotherms to the experimental data using the FIG P program (Biosoft, Cambridge, ... 1) Indeed, there is no correlation between the Ki and EC50 values for the mutant receptors (Fig 4) The ratio of EC50 to Ki is an indicator of efficacy, i.e the likelihood that a receptor will become...

Ngày tải lên: 07/03/2014, 21:20

... weight to asymmetry in these proteins The crystal dimer of the NBD of the maltoseuptake system of Thermococcus littoralis (MalK) consists of two monomers with deviation from perfect twofold symmetry ... from the NBD than ATP Rapid dissociation of hydrolysis product may be expected as part of the kinetics of the transport cycle of the intact transporter Furthermore, the data demon- strate that the ... properties of the NBDs, rather than of their interactions with the TMDs (e.g [50] and see introduction for further references) Recent structural determinations of both intact ABC transporters and NBD...

Ngày tải lên: 08/03/2014, 08:20

... evaluation of the function of the three serine mutants, the kinetic properties of the mutant enzymes were compared to those of the parent enzyme, P-DN42 As shown in Table 1, the Km values of all ... ends of the putative b-strands, lining the face of the predicted active site cleft [15] These proposed structural features of the GT1 motif in GalNAc-T1 are similar to the UBD, raising the possibility ... donor and/ or acceptor specificities Together with information on the three-dimensional structure of the GalNAc-transferases, this will allow an understanding of the catalytic mechanism(s) of these...

Ngày tải lên: 08/03/2014, 10:20

... appear to correspond to the lower two immunobands of wild-type Kv3.1 In both cases, the upper band was darker than the lower band Additionally, the upper band was not visible in the presence of TM ... development 3288 Fig Topological model of Kv3.1 and amino acid sequences of the S1–S2 linker of Kvs (A) Topology of a Kv3.1 monomeric unit Black circles represent the Asn of native N-glycosylation ... utilized in rat brain and infected Sf9 cells, and the role that occupancy of these sites has in the expression of functional Kv3.1s at the cell surface of Sf9 cells Immunoband patterns of wildtype Kv3.1,...

Ngày tải lên: 16/03/2014, 13:20

... in lanes and 11 Ó FEBS 2004 Analysis of the N-myristoylation consensus motif (Eur J Biochem 271) 867 The levels of incorporation of [3H]Leu and [3H]myristic acid into the lower band of the expressed ... incorporated into the upper of the two bands detected with [3H]Leu (lane 6) As there is no Met residue in the mature domain of TNF, this result indicates that the upper band corresponds to the protein ... affects the susceptibility of the protein to protein N-myristoylation In this case, the presence of Lys at position was found to affect the amino acid requirement at position 3, and allowed Lys to...

Ngày tải lên: 16/03/2014, 16:20

... specificity of Ets factors is determined by their site of expression, their selective target sequence recognition, their selective abilities to interact with other transcription factor coactivators, and ... activates the PS1 ERM transactvates PS1 and does not require the )10 Ets element We have begun to identify the Ets factors controlling the expression of PS1 using the yeast one-hybrid selection and the ... using the concentration of protein in the extract as an internal standard The activity of ()119/+178) PS1CAT in the presence of the empty vector was taken as 100% The values represented in the...

Ngày tải lên: 16/03/2014, 18:20

... of the effect of single point mutations on the solution structure of monomeric derivatives, we analysed the 2D NMR spectra of the different variants of mBS Figure shows the expanded regions of ... present A further indication of the similarity of their global fold to that of the parent protein is provided by the 1D H-NMR spectra (data not shown), which display all the characteristic signals ... 4.1 to RNase A, which has a thermal stability higher than that of mBS However, P19A/L28Q-mBS has the same thermal stability of the parent protein, in spite of greater similarity to RNase A To directly...

Ngày tải lên: 16/03/2014, 23:20

... capacity and also modulating the catalytic properties of the enzyme [19] To extend the information about the role of the noncatalytic SBD regions and their effect on the C-terminal CD, we further ... potential target for the manipulation of the level and quality of plant starch However, little is known about the role of SSIII in starch synthesis and the structure–function relationship of this protein ... contains the catalytic site Thus, we evaluated possible intramolecular interactions between the N-terminal SBDs and the C-terminal CD, and their effect on the regulatory properties of SSIII To investigate...

Ngày tải lên: 22/03/2014, 21:20