tet on system toward hepatic differentiation of human mesenchymal stem cells by hepatocyte nuclear factor
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... MF: Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ... separated by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff ... Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
Ngày tải lên :
09/08/2014, 10:23
... Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during ... F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J ... Inhibition of osteoblast differentiation but not adipocyte differentiation of mesenchymal stem cells by sera obtained from aged females Bone 2006, 39:181-188 Page of (page number not for citation...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
Ngày tải lên :
10/08/2014, 05:21
... enhance differentiation of MSCs into insulin producing cells To examine the effects of ECM, FN and LAM on IPC differentiation by MSCs, a four-stage differentiation protocol in suspension pellet ... derived from mesenchymal stem cells in Wharton’s Jelly of the human umbilical cord for transplantation to control type diabetes PLoS ONE 2008, 3(1):e1451 Liu M, Han ZC: Mesenchymal stem cells: biology ... PY: Human bone marrow mesenchymal stem cells can express insulin and key transcription factors of the endocrine pancreas developmental pathway upon genetic and/or microenvironmental manipulation...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
Ngày tải lên :
09/09/2015, 10:07
... biodegradable nanofibers mimicking native bone ECM can enhance the osteogenic differentiation of mesenchymal stem cells and promote bone formation By culturing human MSCs on PLLA electrospun nanofibrous ... images of bone nodules (indicated by arrows) produced by human MSCs of passage on the PLLA nanofibers and TCPS plates (control) in the Ost medium after 21 days of culture The cells on the nanofibrous ... native bone (bone structure, bone cells, bone formation and bone repair), bone grafts and available bone graft substitutes, stem cell response to biomaterial topography, the importance of nanofibers...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
Ngày tải lên :
11/09/2015, 09:16
... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... Kaplan DL 2005;Matrix-mediated retention of adipogenic differentiation potential by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion Biomaterials (31):61676175 83 Mariman ... differentiation of rat bone marrow-derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132(6):1049-1061 Heo JS, Lee MY, Han HJ: Sonic hedgehog stimulates mouse embryonic stem cell proliferation by cooperation of ... IharaWatanabe M, Sakai N, Kogo M, Ozono K, Michigami T: Involvement of nuclear factor I transcription/replication factor in the early stage of chondrocytic differentiation Bone 2007, 41(6):1025-1035 Wang ... hematopoietic stem cells (HSCs) from human umbilical cord blood, and it promotes differentiation of HSCs into progenitor cells by inhibiting ABCG2 expression[56] Page 10 of 17 (page number not for citation...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... evidence of formation of alternate lineages during DE differentiation 128 4.2.6 Gene Expression Analysis of Differentiation Using Microarray Technology 131 Conclusion and ... endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in ... Delta μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
Ngày tải lên :
30/03/2014, 04:20
... examination of the molecular mechanisms underlying hepatic differentiation from stem cells For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte- like cells using ... process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In the case of our study, transdifferentiation, in ... 2, human liver; lane 3, AT-MSC-Hepa; lane 4, human primary hepatocytes The expression level of human hepatocytes was set to 1.0 induced by hepatic differentiation treatment of ATMSCs A number of...
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Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide. ppt
Ngày tải lên :
30/03/2014, 20:20
... variations on levels comparing to the native AtoC, depending on various conditions of DNA-binding and phosphorylation by putative donors All forms were shown to bind at different extension on specific ... (from conditioned media of EA.hy926, HUVEC, HULEC and HUMMEC cell lines) The process of purification has consisted of concentration of the media and three chromatographic steps: Cibacron Blue ... synthesis of deoxyribooligonucleotide conjugates carrying Al(III)–, Zn(II)–, and Co(II)tetracarboxy-Ptcs groups The yields of conjugates were 30–40% Thermodynamics of interaction of Ptc-oligonucleotide...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
Ngày tải lên :
10/08/2014, 05:21
... expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal ... neural differentiation and examined the expression of Oct4 and Nanog During osteogenic differentiation, we noticed a continuous upregulation of Oct4 and Nanog until day followed by downregulation of ... downregulation of both Oct4 and Nanog in 3A6 compared with KP (Figure 2A) Downregulation of the embryonic transcription factors such as Oct4 and Nanog is associated with differentiation of neural stem cells, ...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Ngày tải lên :
16/09/2015, 15:55
... manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno-Free Support Systems and in the Presence of Xeno-Free Proteins 36 Introduction hESC lines ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... approximately out of 10,000 bone marrow cells is a HSC (Spradling et al 2001) Mesenchymal stem cells Mesenchymal stem cells (MSCs) are another well-characterized population of adult stem cells MSCs...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human ... and replated on a fresh MEF feeder layer After days of culture following serial passage (P52 to P55), the degree of spontaneous differentiation of the hESC colonies was assessed by morphological ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton Biol...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
Ngày tải lên :
07/03/2014, 12:20
... progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We observed ... only detected at the core regions [14,15] Localization of RNA Pol II to the LCR was independent of active transcription elongation; the addition of the elongation inhibitor DRB did not affect...
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Atlas of Human Pluripotent Stem Cells doc
Ngày tải lên :
22/03/2014, 09:20
... expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby in 1978 ... and human embryonic stem cells derived These breakthroughs paved the way to the derivation of the first five human embryonic stem cell lines by Thomson et al in Madison, Wisconsin, 1998 (Thomson ... of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration...
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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx
Ngày tải lên :
09/08/2014, 10:20
... Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of hematopoietic lineages, and Wnt3a-specific maintenance of ... transcription factor Twist1 inhibits chondrogenesis J Biol Chem 2006, 281:1381-1388 46 Friedman MS, Long MW, Hankenson KD: Osteogenic differentiation of human mesenchymal stem cells is regulated by bone ... Armstrong MA, Li G: Mesenchymal stem cells in immunoregulation Immunol Cell Biol 2006, 84:413-421 30 Baksh D, Song L, Tuan RS: Adult mesenchymal stem cells: characterization, differentiation, and...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, ... H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; MacroH2A, histone variant MacroH2A; ... cell type of origin used to derive iPSC (c) H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3,...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... Blunn GW, Su Y: Restoration of bone mass and strength in glucocorticoid-treated mice by systemic transplantation of CXCR4 and cbfa-1 co-expressing mesenchymal stem cells J Bone Miner Res 2009, 24:837-848 ... suppression of T cell proliferation through up-regulation of B7-H1 Cell Res 2008, 18:846-857 13 Glennie S, Soeiro I, Dyson PJ, Lam EW, Dazzi F: Bone marrow mesenchymal stem cells induce division arrest ... interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
Ngày tải lên :
09/09/2015, 10:16
... Y Liu, SH Teoh Effects of Strontium on the Proliferation and Bone Forming Capacity of Human Fetal Mesenchymal Stem Cells Seeded onto Scaffolds International Conference on Cellular & Molecular ... bones (Clarke 2008) 2.2 Anatomy of Bone The human skeletal system consists of 80% cortical bone and 20% spongy bone overall, with varying ratios depending on the skeletal site and type of bone ... Expansion in Hypoxiacontinuous on the Osteogenic Differentiation of hfMSC .147 6.5 Discussion .148 6.6 Conclusion .152 Chapter – Conclusion, Considerations and...
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Baculovirus mediated genetic modification of human embryonic stem cells
Ngày tải lên :
11/09/2015, 14:38
... the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human embryonic stem cells by baculovirus vectors had no effects on the cell ... ABBREVIATIONS XIII CHAPTER ONE: INTRODUCTION 1.1 General Introduction 1.1.1 Human Embryonic Stem Cells 1.1.2 Non-Viral Genetic Modification Systems for hES Cells ... benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring pure populations of specific types of ES cell-derived cells, altering antigenicity of cells...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
Ngày tải lên :
09/10/2015, 11:24
... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells into ... bone formed by hMSC A comparison of osteogenesis of hES cells within 2D and 3D culture systems demonstrated that the osteogenic differentiation of hES cells is enhanced in a 3D culture system compared ... understanding of the mechanism underlying the lineage specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived...
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