techniques used for rna quantitation in cell based assays

... was 50°C for and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for min, and finally 95°C for 15 s, 60°C to 95°C for 30 s, and 95°C for 15 s Quantification of the gene of interest ... 400 g for 10 to remove cell contamination, and the supernatant was then stored at -80°C Tissue Processing for histology and immunohistochemical staining Upon completion of the in vivo monitoring, ... until staining Immunohistochemistry staining Immunohistochemical staining of nestin and BrdU were performed to assess the property of NSC in cultures and track the migration and homing of allogeneic...

Ngày tải lên: 18/06/2014, 16:20

... explain the motivation for the present research using an example that was extracted from our training data, as shown in Figure In the alignment of this example, three lines (in dot lines) are used ... consideration behind is in order to generate target function words in a robust manner In order to avoid generating too large a derivation forest for a packed forest, we further used chunk-level information ... the forest-to-string decoder described in (Mi et al., 2008) that makes use of forestbased translation rules (Mi and Huang, 2008) as the baseline system for translating English HPSG forests into...

Ngày tải lên: 30/03/2014, 21:20

... semantic information, but we assume that this information obeys all the same constraints as the semantics for a sign, so in effect such a rule is semantically equivalent to having null elements in ... because they involve negative conditions: for instance, we could not write TFS constraints that absolutely prevent a grammar writer sneaking in a disallowed coindexation by specifying a path into the ... we will simply use relh to stand for such a constraint, intending it to be neutral between, for instance, =q (qeq: equality modulo quantifiers) relationships used in MRS and the more usual ≤ relationships...

Ngày tải lên: 31/03/2014, 04:20

... incubated at 50°C for 60 minutes followed by incubated at 70°C for 15 minutes Single stranded RNA was digested from DNA /RNA hybrid by adding 0.5 µL of RNaseH and incubated at 37°C for 20 minutes http://www.virologyj.com/content/5/1/54 ... suggesting that the ORF58 gene is dispensable for viral replication in cell culture Before performing the remaining experiments, in order to exclude the possibility to affect the packaging of ... L, Sommer MH, Heineman TC, Cohen JI, Kaneshima H, Arvin AM: The ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu mouse...

Ngày tải lên: 20/06/2014, 01:20

... informants were interviewed regarding medicinal plants used in veterinary medicine throughout 2001-2010 Based participant observation and open and semi-structured interviews we obtained information ... regional study in which 64 informants (35 women and 29 men) were interviewed about medicinal plants used in veterinary medicine during 2001-2004, 2006 and 2010 The age of the informants ranged ... Ethnoveterinary medicines used for ruminants in Trinidad and Tobago Prev Vet Med 1998, 35:149-163 10 Lans D, Harper T, Georges K, Bridgewater E: Medicinal plants used for dogs in Trinidad and...

Ngày tải lên: 10/08/2014, 09:22

... (encoding ubiquitin) by semi-quantitative RT-PCR in 17 OCP lines, as well as in WT and cSUC2 lines, and those transformed with pART-uidA-BAR (uidA lines) (Figure 2) In Figure 2, the OCP lines ... di-N-demethylation [1,19] In addition, CYP genes from other organisms have been used for engineering herbicide resistance in plants, as well as for developing new herbicides in conjunction with cognate ... had fewer seeds, resulting in an overall lower seed yield (Figure 3A, B) To gain insight into why fecundity in OCP lines was compromised, scanning electron microscopy was used to analyze flower...

Ngày tải lên: 11/08/2014, 11:22

... concerning faculty development in disaster medicine [13] and no reports that evaluate the teaching performance of instructors in disaster medicine training Well-trained instructors are essential for ... experience conducting instructor training courses Items were chosen to judge the competencies of trainers in preparing, executing and evaluating skills and knowledge for disaster medicine training The ... of performance indicators in evaluating the staff skills during disaster medicine training [7-10] The same approach could be used to compare the teaching skills quantitatively Therefore, in this...

Ngày tải lên: 13/08/2014, 23:20

... differentials in transcripts expressed at low levels This can be achieved in principle by defining a cost factor consisting of the two parameters being tested One form of defining such a cost ... experiments differing in spike-ins with two fold difference in concentration viz., experiments with 3, with and with Probe level data were extracted from Cell files (using tiling coordinates defined by ... than the chip designated the baseline chip, the equation for probesets with value for baseline chip higher can be obtained by interchanging xie and xib For example for a probeset that satisfies the...

Ngày tải lên: 14/08/2014, 14:21

... THE RESULTS OF THE INVESTIGATION Based on the criteria to determine the linguistic devices used for reducing or avoiding conflict in communication, after collecting and analyzing the data, we have ... 4.3 AN INVESTIGATION ON LINGUISTIC DEVICES USED IN HEDGING STRATEGY FOR AVOIDING CONFLICT 4.3.1 An overview Table 4.4: Results of the survey on Vietnamese linguistic devices used in hedging strategy ... conflict in English films? What are linguistic devices used for avoiding conflict in Vietnamese films? What are the similarities and differences in the use of language for avoiding conflict in English...

Ngày tải lên: 14/05/2016, 18:39

... Journal on Applied Signal Processing CALL FOR POSITIONING Development of positioning techniques for cellular networks was mainly motivated by emergency requirements stating that all 911 calls in ... Scientist Researcher His main interests include UMTS radio network planning and development of cellular location techniques Jukka Lempi¨ inen was born in Helsinki, a Finland, in 1968 He received the ... multiple internal UE measurements Thus, the deviations in accuracy of RSCP measurements in different terminals (±10 dB) are averaged, minimizing the in uence of the terminal type on the PCM performance...

Ngày tải lên: 22/06/2014, 22:20

... Diploma in electrical engineering from the National Technical University of Athens, Greece, in 1979; the M.S degree in systems engineering from the Moore School of Electrical Engineering, University ... Electrical Engineering, and Dean of the School of Engineering and Applied Science He has also held visiting appointments at a number of universities, including recently Imperial College, Stanford, and ... Pennsylvania, Philadelphia, in 1980, and the Ph.D degree in electrical engineering and computer science from Princeton University, Princeton, NJ, in 1983 From 1983 to 1986 he was with INRIA, France, and...

Ngày tải lên: 22/06/2014, 23:20

... authors [13-16] Local heparin injection is a well-known procedure in the management of venous thrombosis [13] We avoid the opening of the artery by injecting high dose heparin into the pedicle artery ... Neck 2001, 23:830-835 Chalian AA, Anderson TD, Weinstein GS, Weber RS: Internal jugular vein versus external jugular vein anastamosis: implications for successful free tissue transfer Head Neck ... a squamous cell carcinoma in the mandibular region in January 2007, the patient underwent hemimandibulectomy and primary soft tissue reconstruction with a radial forearm flap in combination with...

Ngày tải lên: 11/08/2014, 20:20

... things such as asking for information, looking for jobs, complaining about something, inviting and so on b Language: Writing letters c Stage of teaching: While-writing d Levels: Except the beginners ... some examples used with this technique: Join these pairs of sentences, using the words in brackets: 1.It was raining heavily We went hunting (Although) 2.I had lived in the North for years I moved ... a variety of techniques from the simplest techniques to the most difficult ones I TECHNIQUES USED TO TEACH : WRITING SENTENCES: In this part, I’m going to describe the techniques used to teach...

Ngày tải lên: 03/06/2013, 01:26

... since it is essential in conducting communication There are several principles in teaching vocabulary in order to make the teaching learning process more interesting They are as follows: It interests ... they are enjoy discovering and understanding interesting things in life Primary students gradually dominate their curiosity in order to form the discipline and implement learning regulations… All ... 33 Findings and discussion of the findings 34 Chapter 3: Some suggested techniques in teaching English vocabulary in primary schools in Quang Ninh Realia 36 Teaching through...

Ngày tải lên: 11/12/2013, 23:53

... deep Further, integrated shrimp farming and mangrove forest modeling was applied in Mekong Delta including study areas, so this method can be used in recognizing shrimp ponds The best indicator with ... (from older images), in the field trip, we identified and draw boundaries of interesting areas that were used for determining the training sites of the classified images in the laboratory Methods ... 20 training areas and 25 sites were chosen for the supervised classification of the Travinh and Camau images, respectively List of training areas in Tra Vinh and Ca Mau were indicated in Table...

Ngày tải lên: 22/12/2013, 06:16

... bilingual student’s knowledge In itself, word twinning is a way involving CS technique for translating terms used to solve issues of applying CS techniques to insert such English terms combining ... phonemes instead of explaining or paraphrasing them in full forms in Vietnamese, their acronyms are acknowledged with meaningful units and copied completely forms instead of indicating by paraphrasing ... students find out steps for shifting terms into TL • Showing students’ habits of using CS in their language learning practice, especially in translation activities Major findings In conclusion, CS techniques...

Ngày tải lên: 29/01/2014, 10:33

Ngày tải lên: 18/02/2014, 11:20

... following contributions: • we formulate the question retrieval task as a phrase -based translation problem by modeling the contextual information (in Section 3.1) • we linearly combine the phrase -based ... of exploring category information for question retrieval in community question answer archives In Proceedings of WWW D Chiang 2005 A hierarchical phrase -based model for statistical machine translation ... contextual information in modeling the translation of phrases as a whole More precise translation can be determined for phrases than for words It is thus reasonable to expect that using such phrase...

Ngày tải lên: 20/02/2014, 04:20

... variety of oral codeinecontaining agents, including acetaminophen plus codeine and proprietary combinations of ∗ ∗ acetaminophen, codeine, and doxylamine (Mersyndol® ) or buclizine (Migraleve® ) ... ergotamine-containing compounds, and ergostine-containing compounds were inconsistent and difficult to interpret This is in part because many of these trials are older and used different dosing ... therapy for patients with status migrainosus (Grade C) • Lidocaine— Intranasal Findings: Limited studies reported lidocaine superior to placebo in relieving acute migraine headache at 15 minutes...

Ngày tải lên: 21/02/2014, 12:20

... were injected in the gonad with 100 lL of saline solution containing dsRNA, or saline solution for the control After dsRNA injection, oysters were maintained in raceways in conditions allowing ... therefore considered as an optimal quantity of dsRNA for in vivo RNAi experiments in adult oysters The inhibition rates for Oyvlg mRNA levels were similar at days and month postinjection, indicating ... presented in this article were therefore performed with a mix of oyvl4-dsRNA and oyvl5-dsRNA, called ‘oyvl-dsRNA’ To validate the in vivo dsRNA injection method in oyster, we used an original technique...

Ngày tải lên: 07/03/2014, 00:20