targeting of viral replication to tumors

Role of pentraxin 3 in shaping arthritogenic alphaviral disease from enhanced viral replication to immunomodulation

Ngày tải lên : 22/09/2015, 15:19

... enhancement of RRV infection However, removal of the C-terminal domain did affect the ability of the N-terminal domain of PTX3 to modulate viral replication, resulting in only partial enhancement of viral ... flow cytometry analysis of peritoneal lavages revealed significantly increased numbers of neutrophils and inflammatory monocytes in the peritoneal cavity of RRVinfected PTX3-/- mice compared to WT ... Role of PTX3 in Alphaviral Infection PTX3 and RRV may promote viral replication processes Moreover, the presence of PTX3 was crucial for enhanced viral replication during RRV infection of WT...

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Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot

Ngày tải lên : 18/06/2014, 22:20

... correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication Journal of Translational Medicine 2011 9:106 Submit your next manuscript to ... our laboratory has demonstrated that the MHC class II transactivator CIITA could block the replication of the human HTLV-2 retrovirus by inhibiting the function of the viral transactivator protein ... relative contribution of Tax-2-endogenous NF-YB in vivo interaction to the functional impairment of Tax-2 is difficult to assess, because NF-YB is an ubiquitous factor and only part of the nuclear Tax-2...

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Báo cáo hóa học: " Modulation of viral replication in macrophages persistently infected with the DA strain of Theiler''''s murine encephalomyelitis virus" docx

Ngày tải lên : 20/06/2014, 01:20

... proving that the effect of hemin on DRAW cells was not due to compound-induced cytotoxicity, but probably resulted from the upregulation of viral replication and/or spread of the infection with ... inhibitor of rhinovirus RNA synthesis [24]; hydantoin, an inhibitor of poliovirus post-synthetic protein cleavages and assembly [25]; levamisole, a compound known to potentiate the antiviral ... treatment of the former also led to an average fivefold increase of virus titers compared to untreated DRAW cells, which might be due to upregulation and/or spread of the infection as a result of the...

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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx

Ngày tải lên : 20/06/2014, 04:20

... correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication Journal of Translational Medicine 2011 9:106 Submit your next manuscript to ... our laboratory has demonstrated that the MHC class II transactivator CIITA could block the replication of the human HTLV-2 retrovirus by inhibiting the function of the viral transactivator protein ... relative contribution of Tax-2-endogenous NF-YB in vivo interaction to the functional impairment of Tax-2 is difficult to assess, because NF-YB is an ubiquitous factor and only part of the nuclear Tax-2...

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Báo cáo y học: "Control of viral replication after cessation of HAART" pdf

Ngày tải lên : 10/08/2014, 05:22

... that a total of 160 patients had stopped HAART for the first time, after a mean of 25 months of successful viral suppression Treatment was stopped under medical guidance, mostly because of toxicity ... Page of We thank the AIDS reference laboratory (ARL) of our institute for the collection and anonymisation of samples Author details Virology unit, Department of Microbiology, Institute of Tropical ... it possible to investigate whether the virus of this patient was less fit in comparison to reference strains or virus of different rebounders Figure clearly shows that the virus of the SC replicates...

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Báo cáo y học: " Management of HIV-1 associated hepatitis in patients with acquired immunodeficiency syndrome: role of a successful control of viral replication" ppsx

Ngày tải lên : 10/08/2014, 05:22

... start of an effective HAART, without interventions finalized to reduce hepatosteatosis Indeed, as previously reported [14], such approaches lead to an improvement of antiretroviral drug tolerability ... 42:425-429 Shah T, Lampiris H, Monto A, Tien PC: Resolution of Hepatitis C VirusInduced Steatosis Improves Tolerability of Antiretroviral Drugs Associated with Hepatotoxicity in an HIV-Infected Individual ... ultrasound Esposito et al AIDS Research and Therapy 2011, 8:9 http://www.aidsrestherapy.com/content/8/1/9 Page of Table Therapeutic history of the patients Antiretroviral drugs Period of therapy ZDV...

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Báo cáo y học: " Dynamics of viral replication in blood and lymphoid tissues during SIVmac251 infection of macaques" docx

Ngày tải lên : 12/08/2014, 23:22

... three viral markers simultaneously to study in detail viral dissemination and the dynamics of viral replication in tissues: viral DNA (indicating dissemination), viral RNA (an indicator of viral replication ... follow-up of 28 days pi The studies used only RNA or total DNA viral markers Viral RNA has classically been used to evaluate viral replication or production, whereas viral DNA is generally used to evaluate ... dynamics of CD4+ T cells, viral replication and dissemination of the virus in the peripheral blood of SIV-infected macaques The dynamics of CD4+ T cells, viral replication and dissemination of the...

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báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx

Ngày tải lên : 20/06/2014, 07:20

... http://www.ovarianresearch.com/content/3/1/12 Page of 14 Figure Design of MSC-AR targeted to tumor marker A) Schematic presentation of Artificial Receptor C6.5 (AR) used to obtain MSC-AR and genomes of adenoviral vectors for AR expression ... software according to the software instruction For comparison of tumor targeting of two cell populations, total intensity of bioluminescence signal acquired from collected tumors were normalized ... Design of transiently targeted and labeled MSC-AR MSC-AR were obtained by transduction of MSCs with adenoviral vectors encoding the artificial receptor (AR) The artificial receptor to target MSC to...

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Báo cáo y học: "Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication" doc

Ngày tải lên : 13/08/2014, 05:21

... with Ti71 rotor (Beckman) at 40,000 rpm to purify virions Viral titers were quantified by RT activity assay [53] To analyze the composition of the virions, equal amount of the viral stocks were ... of the viral DNA to the host DNA; and (3) gap repair, when the 5' ends of the viral DNA are joined to the host DNA by the host DNA repair enzymes Integration of the viral DNA into the host genome ... for targeting of HIV-1 DNA to the chromosome [5-7] The interaction of IN with LEDGF/p75 has been mapped to the residues W131/W132 and the region of I161-E170 in the catalytic core domain of IN...

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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

Ngày tải lên : 16/02/2014, 09:20

... induces apoptosis in a Bax-dependent manner Inhibitor of apoptosis protein family Induction of MOMP following apoptotic stimuli results in the release of cytochrome c and subsequent activation of caspase-9 ... is characterized by the permeabilization of the outer mitochondrial membrane and the release of several pro-apoptotic factors into the cytoplasm For mitochondrial outer membrane permeabilization ... of Bax activation in response to a variety of apoptosis-inducing stimuli Molecular mechanisms of sensitivity to EGFR-TKIs JNK and mitogen-activated protein kinase phosphatase-1 The activity of...

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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt

Ngày tải lên : 16/02/2014, 09:20

... levels of cytotoxicity of individual VP2 and VP3 variants correlate with their ability to induce apoptosis The highly toxic variants of VP2 and VP3 proved to be very potent inducers of apoptosis ... inducers of apoptosis We further examined the character of cell death induced by VP2 or VP3 proteins To assess the contribution of apoptosis to toxicity, we evaluated the cleavage of both effector ... markers during the late stages of MPyV infection However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt MPyV, and replication of the virus mutated in VP2...

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Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

Ngày tải lên : 20/02/2014, 11:20

... Uptake of mitoDC-81 by energized mitochondria An ionselective electrode was used to measure the uptake of mitoDC-81 into rat liver mitochondria To calibrate the electrode ﬁve stepwise additions of ... every 61.5 mV of membrane potential This leads to a millimolar concentration of mitoDC-81 within mitochondria on incubation of cells with high nanomolar to micromolar concentrations of mitoDC-81 This ... alkylation of mtDNA The uptake of mitoDC-81 into cells driven by the plasma membrane potential (Dwp) followed by the further accumulation of mitoDC-81 into mitochondria driven by the mitochondrial...

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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx

Ngày tải lên : 22/02/2014, 04:20

... recognition of terminal tripeptides as peroxisomal targeting signal between yeast and human is due to dierent anities of their receptor Pex5p to the cognate signal and to residues adjacent to it ... extended with the C-terminal 274 amino acids of Mls1p (out of a total of 554), including the terminal SKL Expression of this GFP-Mls1p was compared to that of a control GFP extended solely by SKL ... (Fig 2) Lysates of homogenized wild-type cells were spun to yield an organellar pellet consisting of mitochondria and peroxisomes, and a cytosolic supernatant Equal fractions of each of the protein...

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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt

Ngày tải lên : 07/03/2014, 03:20

... targeted to a speciﬁc location [30] Thus, the acquisition of the dual targeting signal would be a constraint compared to targeting of location-speciﬁc proteins to avoid loss of targeting to the ... as in mitochondrial targeting signals) and may be slightly enriched in hydrophobic residues In yeast, proteins targeted to mitochondria Fig Overview of dual targeting of proteins to mitochondria ... for dual targeting, and also what is required to avoid mis-sorting of proteins between mitochondria and chloroplasts The structures of binding of targeting peptides to mammalian and plant Tom20s...

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Báo cáo khoa học: The C-terminus of viral vascular endothelial growth factor-E partially blocks binding to VEGF receptor-1 docx

Ngày tải lên : 23/03/2014, 07:20

... specificity of the viral VEGFs It has been hypothesized that the inability of viral VEGF to bind VEGFR-1 may be due to the absence of a functional groove on the receptorbinding face of the homodimer ... receptor-recognition profile of viral VEGF The C-terminal residues may therefore form a direct physical block, preventing access of the VEGFRs to the receptor-binding face Curiously, replacement of ... residues of the VEGF-A mutant, due to the influence of adjacent residues or other structural features of VEGF-A, are not orientated towards the receptor-binding face and are therefore unable to influence...

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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx

Ngày tải lên : 30/03/2014, 11:20

... hepatocytes (Fig 2C) Effects of activity and concentration of phosphorylase-a on glycogen synthesis To test the role of the phosphorylated form of phosphorylase independently of changes in total ... ? hepatocytes To test the role of phosphorylase activity, as distinct from the phosphorylation state of the enzyme, we used 1,4-dideoxy-1,4-imino-d-arabinitol (DAB), a potent inhibitor of phosphorylase ... contributory factor to the high control coefﬁcient of phosphorylase on glycogen synthesis because using a potent inhibitor of phosphorylase (DAB) that does not promote conversion of phosphorylase-a to -b...

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Báo cáo khoa học: "Analysis of limb function after various reconstruction methods according to tumor location following resection of pediatric malignant bone tumors" doc

Ngày tải lên : 09/08/2014, 03:21

... to make the limb a total of 43.5 mm longer As of years postoperatively, however, the limb-length discrepancy had reached 20 mm, with a range of motion of 45° during flexion of the knee joint Apparent ... from that in adult cases To solve this problem, we attempted to classify reconstruction of the lower limbs into types based on the sites of tumor location on MRI Type I tumors were those located ... resection of the tumor Partial skin necrosis occurred postoperatively at the insertion site of one of the pins In Case 8, the Page of skin became partially necrotic at the frontal aspect of the...

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báo cáo khoa học: " Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles" doc

Ngày tải lên : 11/08/2014, 00:22

... 331(2):527-535 Suikkanen S, Aaltonen T, Nevalainen M, Valilehto O, Lindholm L, Vuento M, Vihinen-Ranta M: Exploitation of microtubule cytoskeleton and dynein during parvoviral traffic toward the nucleus ... attached to a deleted CPV VP2 polypeptide of 14 amino acids [33] Together this suggests the possibility of replacement of the first 40 amino acids to be used for designing novel vectors for various ... fluorescence autocorrelation Figure curves of of fluorescent recombinant proteins The effect of trypsin on the fluorescence autocorrelation curves of the fluorescent recombinant proteins Normalized autocorrelation...

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Báo cáo y học: " Variability in a dominant block to SIV early reverse transcription in rhesus monkey cells predicts in vivo viral replication and time to death" pot

Ngày tải lên : 12/08/2014, 04:20

... population Page of 11 could impact the quantitation of the relative susceptibility of PBMC to SIV infection To rule out a contribution of these well-defined factors to the variability of rhesus monkey ... stage of viral replication prior to viral gene expression SIV resistance in rhesus monkey cells correlates with resistance to primate immunodeficiency viruses, but not to other viruses To determine ... in SIVsmE660 replication in resistant cell lines as we added increasing quantities of VLPs In fact, the addition of 150 ng of VLPs led to the complete loss of resistance to SIV replication in...

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Báo cáo y học: " Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P" pptx

Ngày tải lên : 12/08/2014, 04:20

... Binding of EGSs to the target mRNA results in a helix structure of bp that is equivalent to the D stem of a tRNA, which usually has bp Our choice of a D stemequivalent structure of bp instead of bp ... of UL49 expression in the EGS324-transfected cells is not necessarily due to specific EGS-directed RNase P cleavage of the target mRNA, but is due to other effects of the EGS on viral lytic replication, ... expression of viral immediate-early genes To exclude these possibilities and further determine the antiviral mechanism of the EGSdirected cleavage, we examined the expression of other viral genes...

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