targeting of tumor speci c dysfunctions in cells cycle control or apoptosis by adenovirus mutants
Báo cáo khoa học: "Identification of Domain-Specific Senses in a Machine-Readable Dictionary" potx
Ngày tải lên :
30/03/2014, 21:20
... of words in EDR Japanese dictionary The Mainichi documents are organized into 15 categories We selected categories, each of which has sufficient number of documents All documents were tagged by ... Tagger (Schmid, 1995) Table shows 20 categories, the number of training and test documents, and F-score (Baseline) by SVM For each category, we collected noun words with more than five frequencies ... considerable amount 2.2 Computation of rank scores of hand-labeling In this paper, we focus on domain -speci c We note that text classification accuracy used in sesenses and propose a method for...
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Báo cáo khoa học: Characterization of testis-specific serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc
Ngày tải lên :
23/03/2014, 11:20
... absence of a cofactor As TSSK3 is a testis -speci c kinase, such a cofactor is not necessarily expressed in the A14 cells that we used to analyse activation of TSSK3 in vivo by insulin which may ... activation of other kinases is governed by its cellular location For example, in insulin-induced activation of PKB ⁄ Akt the insulin-induced transient increase in 3¢-phosphorylated inositide lipids ... thereby replace the requirement for T168 phosphorylation The kinase activity of these mutants was compared with a classical kinase-dead mutation in which the critical lysine of the ATP-binding pocket...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... 3D for brain and uterine ECs; data not shown for others), but not by nonendothelial NIH3T3 cells (data not shown), indicating that the cells maintained the physiological characteristic of acetylated-LDL ... negative control Transfection of plasmids harboring a GFP-expressing cassette into tsA58T-expressing endothelial cells Transfection was performed using Lipofectamine LTX and pcDNAÔ6.2-GW ⁄ miR-neg control ... transgenic mice were back-crossed five times or more with B6 mice and used for analysis For genotyping, PCR and ⁄ or X-gal staining of tail tips were performed ESCs were maintained on a layer of irradiated,...
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Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc
Ngày tải lên :
19/02/2014, 07:20
... AATATACGGTTCATGGCAATACTGT CTACCCCGGCGCTGGAGTCAAC TCAGATGTTTTGCCACAAAGAGGTGCCTCCT CGA GCT TGC TTT ACA TCA GCC TGT GTC ATC TAG GGT GAA GCC GGC TTC AAG GTT ACT TCG CGG TGG GGC ACC AGC TTG TAA TGG GGC GAA TCG ... using the optimal number of PCR cycles (midpoint of the logarithmic range) This was 27 cycles (in the case of SERCA 2a), 28 cycles (CaN), 35 cycles (CaMKII) or 25 cycles (GAPDH), consisting of ... CaN CaMKII COX I COX Vb F1-ATPase b GGC TCC ATC TGC TTG TCC ATG GAA GCG GTT ACT CCA GTA TTG GAG CTG AAC GGG AAG CTC ACT GG GTG AGG GAG ATG CTC AGT GTT GG AGTAACAATTTTCAGTGCTCCAAAC AATATACGGTTCATGGCAATACTGT...
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Tài liệu Báo cáo Y học: Use of site-specific recombination as a probe of nucleoprotein complex formation in chromatin Micha Schwikardi and Peter Droge ¨ potx
Ngày tải lên :
22/02/2014, 07:20
... depending on the presence or on the accessibility of accessory sites in res, gd102NLS employs two recombination pathways in eukaryotic cells that can be distinguished by the resulting products In order ... is present in CHO cells throughout the time course of our experiments [10], neither changes in chromatin structure occurring during the cell cycle nor the passage of a replication fork render ... particular acetylation of the N-terminal tails of histones are thought to render chromatin more accessible for DNA-binding proteins In fact, DNA transactions such as V(D)J recombination and transcription...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
Ngày tải lên :
15/03/2014, 10:20
... C, but are due to the absence of GMP binding, as confirmed by competitive fluorescence measurements Binding and dynamics of fluorescent nucleotide analogs To investigate the binding of fluorescent ... nucleotide binding pocket and the protein surface may counterbalance the changes in conformational flexibility imposed by nucleotide binding, while in the case of hGBP5, conformational restrictions ... results), which resulted in a 2.5-fold increase in speci c GTPase activity and increased formation of GMP during hydrolysis Thermodynamics and stoichiometry of nucleotide binding We used ITC to investigate...
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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
Ngày tải lên :
16/03/2014, 22:20
... (crabp1a; 3¢-RACE: 5¢-CCC AACTTCGCCGGCACCTGG-3¢, 5¢-TGAAAGCTCTCG GCGTAAAC-3¢; 5¢-RLM-RACE: 5¢-GAGCTTTCAGA AGTTCGTCG-3¢, 5¢-GAATCTCCACATGCGGTTTG-3¢) and a zebrafish genomic DNA sequence assembly (BX663612) ... 5¢-CGGCCATCGACGGTCTC-3¢) Three 3¢-RACE cDNA and 5¢-RLM-RACE clones of each gene were sequenced and the complete cDNA sequences of crabp1a and crabp1b genes were determined by aligning and combining ... which harbors the coding sequence of another gene similar to the mammalian CRABPI (crabp1b; 3¢-RACE: 5¢-GCTAACAGATCAATAGGCTTC-3¢, 5¢-GATTTGAA AGCAAGAGGGTC-3¢; 5¢-RLM-RACE: 5¢-TTAGACGC AGCCGCACAAG-3¢,...
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Báo cáo hóa học: "Research Article Polarimetric Kronecker Separability of Site-Specific Double-Directional Channel in an Urban Macrocellular Environment" pptx
Ngày tải lên :
21/06/2014, 22:20
... separated for each polarization pair By using the proposed sum of Kronecker products, the error is investigated in this section 5.1 Criterion The ergodic mutual information introduced in Section 3.3 ... pair-wise Kronecker products approximation, which the authors shortly call as the “sum of Kronecker products,” to investigate the Kronecker separability of the joint correlation matrix for each channel ... vectors for the BS and MS are assumed to be unchanged over all directions according to [22] 4.2 Polarimetric Kronecker Product Approximations In zeromean complex circularly symmetric Gaussian channels,...
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Báo cáo hóa học: " Research Article Tools for Protecting the Privacy of Specific Individuals in Video" potx
Ngày tải lên :
22/06/2014, 23:20
... “Comparative performance of different skin chrominance models and chrominance spaces for the automatic detection of human faces in color images,” in Proceedings of the 4th IEEE International Conference ... bound of using noisy constraints Figure demonstrated the performance with aforementioned three types of constraints In contrast to the accuracy of 0.7375 without any constraints, the accuracy of ... on Advances in Signal Processing obscure human faces as those appearing in TV news But in this work, since we are interested in privacy protection for medical applications, obscuring faces might...
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Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot
Ngày tải lên :
14/08/2014, 19:22
... with ethidium bromide Specificity of PCR products was controlled using an internal 33P-5'-end labeled oligo-probe specific of human IL3 coding sequence (ACGGCCGCACCCACGCGACA), in Southern blot analysis ... multiple cloning site of pCEP4), which resulted in a PCR product specific of the gene inserted in the pCEP4 Nested PCR was performed using sense (CCAAACTCAATGTATCTTATCATGTCT) and anti-sense (TCAGATTCTAGAAGCTTGGGT) ... production of a functional growth factor in specifically CD117+ targeted cells, mediating an in vitro biological effect on hematopoiesis [14] Since our previous report evidenced interaction of...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
Ngày tải lên :
07/03/2014, 16:20
... in a b-counter after addition of scintillation fluid Nonspeci c binding was determined in the presence of lm unlabeled agonist and subtracted from the total binding to calculate the speci c binding ... Discussion Phosphorylation of serine or threonine residues in the C- terminus of GPCRs by second messenger kinases or speci c GRKs is a requirement for receptor sequestration [23] However, the context ... 7.2) and incubated with the indi- 137 Role of helix and C- termini in bradykinin receptors cated concentration of [3H]agonist in 0.3 mL incubation buffer on ice to reach equilibrium binding To start...
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Báo cáo khoa học: "Joint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue Systems" doc
Ngày tải lên :
07/03/2014, 22:20
... acts Each dialogue act class typically contains several more speci c dialogue acts that include domain -speci c semantics (for example, there are 29 subtypes of wh-questions that can be performed ... number of dialogue acts per utterance is 2.3 The dialogue act annotation scheme uses a total of 77 distinct labels, with each label corresponding to a domain -speci c dialogue act, including some ... dialogue acts The annotation also contains for each dialogue act the corresponding segment of the input utterance The dataset contains a total of 1,579 utterances Of these, 1,204 utterances contain...
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Báo cáo khoa học: Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides doc
Ngày tải lên :
16/03/2014, 16:20
... Immunofluorescence microscopy Cells grown in coverslips were washed in NaCl/Pi, fixed in acetone at )20 C for min, and incubated in NaCl/Pi containing 3% (w/v) BSA for h at 37 C to block nonspeci c ... network of CHO-K1 cells Biochem J 342, 633–640 19 Friedrichson, T & Kurzchalia, T.V (1998) Microdomains of GPI-anchored proteins in living cells revealed by crosslinking Nature 394, 802–805 20 Cochet, ... mica Clı´ nica, Facultad de Ciencias Quı´ mica, UNC, Argentina) The authors also thank G Schachner and S Deza for technical assistance with the cell culture and C Mas for excellent assistance...
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Báo cáo khoa học: Structural basis for stereo-specific catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx
Ngày tải lên :
23/03/2014, 13:20
... refinement Unsuccessful approaches HGDH crystallised in the presence of NAD+, 2-oxoglutarate or oxalylglycine showed no corresponding electron density An increase in the concentration of these compounds ... dehydrogenase [43]) Interdomain cleft closure and catalysis The flexible character of the interdomain cleft permits the exchange between binding of both substrate and cofactor, their catalytic turnover ... source for Peptostreptococcus asaccharolyticus Arch Microbiol 142, 128–135 ´ Retey J & Robinson JA (1982) Stereospecificity in Organic Chemistry and Enzymology Verlag Chemie, Weinheim Berk H, Buckel...
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Báo cáo khoa học: "Unsupervised Discovery of Domain-Specific Knowledge from Text" pptx
Ngày tải lên :
23/03/2014, 16:20
... amount of data The same need for basic knowledge appears in all domains, though In music, musicians play instruments, in electronics, components constitute circuits, circuits use electricity, etc ... base” for baseball While in NE tagging each word has only one correct tag in a given context, we have hierarchical classes: an entity can be correctly labeled as a player or a quarterback (and ... scored according to the majority of annotators’ decisions The model and baseline propositions for the full data set are both judged highly sensible, achieving accuracies of 96.6% and 92.1% (cf...
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Báo cáo khoa học: "Fully Unsupervised Discovery of Concept-Specific Relationships by Web Mining" pdf
Ngày tải lên :
23/03/2014, 18:20
... relations involving a speci c concept 5.2 Collecting contexts For each concept word S, we search the corpus for distinct contexts in which S appears (For our purposes, a context is a window with exactly ... Other words in the class can thus be generated by searching a sub-corpus of documents including at least two concept words for those words X that appear in a sufficient number of instances of both ... same class Call these concept words (For instance, if our example words were France and Angola, we would generate more country names.) Step 2: For each concept word, collect instances of contexts...
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Báo cáo khoa học: A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situ pdf
Ngày tải lên :
30/03/2014, 04:20
... primer, 5Â-GCGCAAGCGCTTACGAA AATTCGTGCCGAAACTTGCA-3Â; 5T antisense primer, 5Â-TTTTCGTAAGCGCTTGCGCTGCAAGTTTCGGCAC GAA-3Â; 2.5T sense primer, 5Â-GCGCAAGCGCTTACG ACTTAAAAAAATTGGTCAGAAAATCCAGG-3Â; 2.5T ... were introduced into FRET DNA fragment by using the following primers: 5Â-GCTTCAGCTGGGATCCGGTGGTATGGTGAG CAAGG-3Â; and 5Â-CCAGATCGCGGCCGCTTAGTGG TGATGATGGTGGTGATGATGCTTGTACAGCTCGT CC-3Â Following ... Medical Center, Nashville, TN, USA) [45] The Cerulean gene was subcloned from pECFP -C1 with the primers 5Â-GCAGGTGTGAATTCCATGGTGAGCAAGGGCGAG GAGC-3Â and 5Â-CCAGATCGCGGCCGCCTTGTACAG CTCGTCATGCCGAGAG-3Â;...
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Báo cáo khoa học: " Kinetic modeling of tumor growth and dissemination in the craniospinal axis: implications for craniospinal irradiation" pps
Ngày tải lên :
09/08/2014, 10:21
... cells cleared from the CSF This is accounted for in the model by incorporating modulating efficiency factors for transfer of viable cells from the CSF to solid tumor and from solid tumor to CSF, ... lead to clinical complications, implying that kshed for these tumors is exceedingly low For example, CSI was once the treatment of choice for intracranial germinomas [22,23] However, more recent ... craniospinal axis has been made This may not be the case in all circumstances [26] Incorporation of changes in cell density in the different compartments could be incorporated in future versions of...
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Báo cáo y học: "The role of tumor necrosis factor-alpha in systemic lupus erythematosus" pdf
Ngày tải lên :
09/08/2014, 10:22
... complications [33,34] Tumor necrosis factor can deteriorate murine lupus Drug-induced lupus and lupus nephritis under tumor necrosis factor blockade In contrast to the findings in NZB/W mice, in ... indirect in part, involving cytokines such as IL-6 for B cells (B) and IL-18 for cytotoxic T cells (Tc) On the other hand, chronic TNF exposure leads to the disassembly of T-cell receptors (TCR), ... Most clinical manifestations of SLE are the consequence of inflammatory changes subsequent to immune complex formation with Fc receptor and complement activation In the sera of SLE, in contrast...
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Báo cáo khoa hoc:" Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes" ppt
Ngày tải lên :
09/08/2014, 18:21
... procedure for screening recombinant plasmid DNA, Nucleic Acid Res 19 (1979) 62416247 [3] Chevalet C. , Corpet F., Statistical decision rules concerning synteny or independence between markers, Cytogenet ... specic primers could be obtained As a result, we decided to use primers specic for a microsatellite derived from a phage vector containing the bovine gene [12] and which serves as a reference for ... consisting of pools of plates, columns and rows using a 3D strategy PCR-based screening was performed as described in Eggen et al (submitted) The BAC clone DNA mini-preparations were performed according...
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