targeting cancer with small molecular weight kinase inhibitors

Ngày tải lên: 12/01/2014, 22:19

... non -small cell lung cancer Cancer Res 2004, 64:5564-5569 32 Offersen BV, Pfeiffer P, Andreasen P, Overgaard J: Urokinase plasminogen activator and plasminogen activator inhibitor type-1 in nonsmall-cell ... papers examining lung cancer tissues and/or lung cancer cell lines using DNA microarrays and/or qPCR have been published e.g [1-3,6-10,19,20], lung cancers still lack reliable molecular markers [20] ... non -small cell lung cancer subtypes Lung Cancer 2009, 63:32-8 Kim B, Lee HJ, Choi HY, Shin Y, Nam S, Seo G, Son DS, Jo J, Kim J, Lee J, Kim J, Kim K, Lee S: Clinical validity of the lung cancer...

Ngày tải lên: 15/02/2014, 04:20

... with water-jacketed cell holder and a Julabo F25 circulator bath with programmable temperature controller The actual temperature inside the quartz cell (with path length of mm) was measured with ... molecular mass to TetR, is a homodimeric molecule with a larger domain responsible for the cAMP binding and a smaller domain, which possesses HTH structure, responsible for the interactions with ... affected by pH change with increasing temperature A comparison of calorimetric enthalpy and denaturation temperature for the protein in the absence of Tc, in buffer with and without Mg, does not...

Ngày tải lên: 20/02/2014, 02:21

... number-average molecular weight is described by Flory-Fox equation:   Tg Tg  K Mn (3)  where Tg is a glass transition for polymer with the infinite number-average molecular weight, K is an ... number-average molecular weight of polymer 2.1 Thermal properties of Kraft lignin extracted with organic solvents In this sub-chapter, an example of the effect of various extraction solvents on molecular weight ... properties on the value of the molecular weight of polymer, the polymer processing methodology and the presence of additives in the polymer mixtures Effect of molecular weight on glass transition...

Ngày tải lên: 06/03/2014, 22:20

... protein kinase inhibitor of cAMPdependent protein kinase J Biol Chem 272, 18169–18178 Gamm, D.M & Uhler, M.D (1995) Isoform-specific differences in the potencies of murine protein -kinase inhibitors ... contained large amounts of random coil and turn structures, with a small amount of a-helix and b structures However, this was compatible with previous reports [23] that minimal structure should ... protein kinase J Biol Chem 266, 11158–11162 Scarpetta, M.A & Uhler, M.D (1993) Evidence for two additional isoforms of the endogenous protein kinase inhibitor of cAMP-dependent protein kinase...

Ngày tải lên: 07/03/2014, 15:20

... lower temperature The ternary complexes of t-S1 and Nt-S1 with ADP and phosphate analogs Previous DSC studies performed with skeletal S1 and with D discoideum myosin head fragments have shown that ... similar to those obtained earlier with uncleaved S1 [15,16,36] Thus, the ternary complexes of Nt-S1 with ADP and Pi analogs are as stable as the complexes obtained with control uncleaved S1 as they ... transition with maximum at 58 °C characteristic for control uncleaved S1 in the S1ÆADPÆVi complex turns into transition with maximum at 53.1 °C which corresponds to Nt-S1 in the ternary complex with...

Ngày tải lên: 17/03/2014, 10:20

... z z y x y x (a) (b) (c) Fig (a) The 1-form dx, with surfaces perpendicular to the x axis and in nite in the y and z directions (b) The 1-form dz, with surfaces perpendicular to the z -axis and ... convention are that it is more consistent with the mathematical point of view, in which basis vectors and forms are abstract objects not associated with a particular system of units, and that ... surface, where each tube is weighted positively if its orientation is in the direction of the surface's oriention, and negatively if opposite This is illustrated in Fig 3b As with 1-forms, 2-forms...

Ngày tải lên: 24/04/2014, 16:51

... Thermogravimetric Analysis (TGA) • thermobalance to monitor sample weight as a function of temperature • weight calibration known weights using • temperature calibration based on ferromagnetic transition ... to the sensor * small sample masses and low heating rates increase resolution, but at the expense of sensitivity Al EYP 2006 Pt alumina Ni Cu quartz Purge Gases Sample may react with air - oxidising ... heat capacity, slow accurate heating rates (0.5–2.0 °C/min), and similar sample and reference pan weights calibrants • • • • • • high purity accurately known enthalpies thermally stable light stable...

Ngày tải lên: 24/05/2014, 20:41

... tyrosine kinase inhibitor, targets Src kinase, in addition to BCR-Abl, c-KIT, PDGFR and ephrin-A receptor kinases It is the most potent Src kinase inhibitor currently in clinical development with ... EphA2 kinase EphA2 IP: p-EphA2 FAK kinase p-FAK py861 p-FAK py397 α-tubulin 48 Hr 24 Hr Hr Hr 0.5 Hr C C EphA2 IP: EphA2 kinase EphA2 IP: p-EphA2 Figure IPblotting for Src kinase, phospho-Src kinase ... A Src kinase p-Src py418 EphA2 IP: EphA2 kinase EphA2 IP: p-EphA2 FAK kinase p-FAK py861 α-tubulin B HT144 - Lox-IMVI + - Malme-3M Sk-Mel-5 Sk-Mel-28 - + - + - + + Dasatinib 100 nM Src kinase...

Ngày tải lên: 18/06/2014, 15:20

... at 50–150°C Without As4 At first, we confirmed the surface structure transition by applying voltage at low temperatures After the InAs WL growth, we applied voltage on the InAs WL without As4 Figure ... Nanoscale Res Lett (2010) 5:1930–1934 Experimental Details All experiments were carried out with the STMBE system We used a GaAs (001) just substrate All samples were prepared at same procedure: ... structure (45 50 nm2) appeared around the hole structure as shown in Fig 1(c’) This ring structure was smaller than that of Fig 1(b’) This result indicated that the size of the surface structure transition...

Ngày tải lên: 21/06/2014, 08:20

... rice starch-water systems within the temperature range studied *Mean ± standard deviation (n = 2) Means within a column (compared within same starch to water ratio) with the same letter are not ... phase transitions were broad and flatten with Tcxo of ~119oC for 1:1, sharpen prominently and bigger for 1:2 but became slightly smaller and broader for 1:3, with Tcxo of ~89oC and 85oC, respectively ... cooling T cxo oC Tcx oC ΔH J/g *Mean ± standard deviation (n = 2) Means within a column (compared within same starch to water ratio) with the same letter are not significantly different at p < 0.05...

Ngày tải lên: 07/08/2014, 14:20

... autoimmune and inflammatory diseases as well as certain cancers [13] NF-κB is highly activated in the synovial tissue of patients with RA [14,15], with IKK-β being a key regulator of synovial inflammation ... pre-incubated for hours with NBD or MUT peptides (50 µM) in medium containing 0.5% FCS and stimulated with IL-1-β (2.5 ng/ml) After 30 minutes of stimulation, cells were washed twice with ice-cold phosphate-buffered ... obtain macrophages, with half of the medium refreshed every days [27] Macrophages were preincubated for hours with NBD peptides (50 µM), and cells were stimulated for 24 hours with rhIL-1-β (2.5...

Ngày tải lên: 09/08/2014, 08:22

... control medium (10 ml) with or without IL-1+OSM (1 and 10 ng/ml, respectively) (in triplicate) was then added (day 0) At day 7, culture supernatants were harvested and replaced with fresh medium containing ... proteoglycan was released from the cartilage stimulated with IL-1+OSM (Figure 1) in line with previous findings [14] This was concomitant with the rapid and high levels of induction for ADAMTS-4 ... cartilage resorption to compare the temporal expression of metalloproteinases and their inhibitors and correlate this with pro-collagenase activation and aggrecan and collagen release Materials and methods...

Ngày tải lên: 09/08/2014, 08:22

... useful inhibitors of the tyrosine kinase activity of c-Met combined with radiation have not been previously tested in GBM models In the work presented here, a novel inhibitor of c-Met tyrosine kinase, ... cells treated with MP470 (10 μM) for hr showed reduced expression of pAkt protein those mice with MP470, irradiation, or both, with animals per group Treatment started on day 25 with MP470 which ... Factor/MET Signaling in Cancer Clin Cancer Res 2008, 14:5941-5946 Lal B, Xia S, Abounader R, Laterra J: Targeting the c-Met Pathway Potentiates Glioblastoma Responses to γ-Radiation Clin Cancer Res 2005,...

Ngày tải lên: 09/08/2014, 10:20

... and 3.08° respectively with neutral suspension casting The SRD with digital scanning of Discussion Digital scanning and neutral suspension casting present the clinician with two distinctly different ... technique In rater a similar pattern was noted, with mean measures of forefoot to rearfoot alignment higher with neutral suspension casting than with digital scanning, but the SRD remaining proportionately ... foot were taken by rater followed by rater with a minute rest period between each cast Three digital images were then captured by rater followed by rater with a 20 second rest period between image...

Ngày tải lên: 10/08/2014, 21:24

... tumor growth models with the breast carciTable 1: Kinase inhibition profile of ABT-869 (with permission adapted from Molecular Cancer Therapeutics 2006;5:995–1006) Related RTKa Kinase KDR FLT1 FLT4 ... in Combination With Tarceva in Subjects With Solid Tumors Phase Study of ABT-869 in Subjects With Solid Tumors Phase Study of ABT-869 in Subjects With Advanced Non -Small Cell Lung Cancer (NSCLC) ... Subjects With Advanced or Metastatic Non -Small Cell Lung Cancer (NSCLC) Phase Study of ABT-869 in Subjects With Advanced Renal Cell Carcinoma Who Have Previously Received Treatment With Sunitinib...

Ngày tải lên: 10/08/2014, 22:20

... Phosphatidylinositol 3 -kinase inhibitors: promising drug candidates for cancer therapy Cancer Sci 2008, 99:1734-1740 32 Kong D, Yaguchi S, Yamori T: Effect of ZSTK474, a novel phosphatidylinositol 3 -kinase inhibitor, ... cells were also cultured with M-CSF (10 ng/ml) for two days and then with M-CSF and sRANKL (100 ng/ml) for another three days The inhibitors were added simultaneously with sRANKL a) and c) TRAP-positive ... RANKL with or without ZSTK474 pretreatment c) NFATc1 localization was visualized using immunofluorescence microscopy in RAW264.7 cells cultured with sRANKL and TNF-α for 24 hours Treated with...

Ngày tải lên: 12/08/2014, 12:20

... the main pathways involved in this Ca2+-sensitization is the RhoA/Rho -kinase pathway [7,6] Activated Rho -kinase interferes with the equilibrium of MLCK and MLCP activities by phosphorylating and ... Rhokinase inhibition [14] The enhanced contribution of Rho -kinase to airway responsiveness could involve increased expression of RhoA, as protein levels of this upstream activator of Rho -kinase ... to a certain agonist is associated with the extent to which the agonist is dependent on Rho -kinase for its contractile effect Conclusion Inhalation of the Rho -kinase inhibitor Y-27632 causes a...

Ngày tải lên: 12/08/2014, 16:20

... type of cancer, and not any other types of cancer or normal tissues [6], is part of the domain knowledge Hence the term 'marker' – for genes that mark the particular cancer associated with them ... shown in Table We observe that for datasets with large K (such as BRN, GCM, and NCI60) the range R( x0,k ) is greater than in case of datasets with smaller K Looking at the maximum and minimum ... (small) percentage of the total variance, which is most likely caused by noise or variance within class (i.e., 'non-relevant' variance as opposed to the first type of variance since variance within...

Ngày tải lên: 12/08/2014, 17:20