table d 2 nondefault properties of the books table

... Observed item-score function; Dashed line = Expected item-score function under the Graded Response Model; Dashed/dotted lines indicate 95%variability envelopes under the Graded Response Model the DIF ... recoding the reverse worded items, sum scores may range from to 30; the higher the sum score, the higher the level of depression In the present study, a Dutch version of the EDS was used The EDS ... are the measurement properties of the EDS for screening depression? If the estimated IRT model fits the data adequately, the parameters from the IRT model can be used to explore and describe the...

Ngày tải lên: 11/08/2014, 15:22

... TGBp2 is able to bind viral RNA and to gain insight into the RNA-binding properties of TGBp2, we prepared unfused TGBp2 [9] and His6-tagged TGBp2 of BaMV to characterize their RNA-binding properties ... non-viral Figure The RNA-binding properties of unfused TGBp2 The RNA-binding properties of unfused TGBp2 A Effect of salt concentration on the RNA-binding activity of unfused TGBp2 After UV-crosslinking ... plasmid as a template [21 ] and the two primers, M2F and M2R (Table 1) The DNA fragment was then digested with HindIII and BamHI and cloned into pT7-6 [22 ] The His6-TGBp2 with Arg- or Lys-to-Ala substitutions...

Ngày tải lên: 12/08/2014, 04:21

... Diffusivity α⋅106 m2/s 1 .24 1 1 .24 6 1 .26 3 1 .25 4 1 .24 9 1 .26 5 1 .25 3 Volumetric thermal capacity ρc⋅10-6 J/m3K 2. 16 2. 17 2. 18 2. 16 2. 20 2. 18 2. 18 Mean temperature Tm [°C] 29 . 82 28.96 32. 27 31.11 30.56 ... additional treatment (Table 4) Table Thermal properties of the calcareous stone of Alberobello Provini A B C Average values: Conductivity λ W/(m K) 2. 681 2. 701 2. 610 2. 548 2. 678 2. 656 2. 646 Diffusivity ... 10 days The data picked up in situ allowed determining through the average and black-box methods, the thermal conductance of the wall With this particular type of construction of the monitored...

Ngày tải lên: 05/09/2013, 14:58

... for ADHD and had a symptom severity of at least 1.5 standard deviations (SD) above norm values for the ADHDRS (ADHD subscale of the SNAP in Study 3) The diagnosis was confirmed using the Kiddie-Schedule ... health and is distinct from symptoms of the disorder and objective functional outcomes (Wallander et al 20 01) It strongly depends on the subjectively perceived impact of the disorder (and of the respective ... and quality of life of children suffering from attention deficit hyperactivity disorder (ADHD) Eur Child Adolesc Psychiatry 16:316– 326 Harpin VA (20 05) The effect of ADHD on the life of an individual,...

Ngày tải lên: 12/02/2014, 19:20

... W, Downes CS & Kurth MJ (20 07) Deactivation of Src family kinases: hypothesis testing 4116 19 20 21 22 23 24 25 26 27 28 29 30 31 using a Monte Carlo sensitivity analysis of systems-level properties ... states, whereas the dotted black line shows unstable steady states (steady state values are designated as s1SS) Dependence of the oscillation amplitude (C) and period (D) on the active Csk concentration ... and B N Kholodenko Fig S2 Proximity of the Src initial state to the excitability threshold decreases the lag period Fig S3 Complete numerical investigation of the dependence of the Z-shaped QSS...

Ngày tải lên: 18/02/2014, 11:20

... and PPi did not affect the activity when added to the standard assay at or 20 mm Identification of the native glucose dehydrogenase in P torridus In order to identify the native GdhA in P torridus ... abolished by the chelating agent EDTA However, the addition of Zn2+ did not affect the specific activity of the enzyme, and even high concentrations of EDTA (20 mm) could not decrease the activity of ... observed with mm NaCl, MgCl2, MnCl2 or CaCl2 EDTA added at up to 10 mm caused no loss of activity However, the addition of ZnCl2 to the incubation buffers showed a marked effect on the stability of...

Ngày tải lên: 19/02/2014, 16:20

... reduced form of the enzyme For the reduced form, the Soret band can be observed at 415 and 4 42 nm, and for the oxidized form at 403 and 422 nm The b–band can be seen at 517 nm and the a–band at ... Correspondingly, the band at around 20 40 cm)1 of the reduced form could be attributed tentatively to the C–N stretching of a nonbridging cyanide ligand of heme a3 In the spectra observed for the critical ... cm)1 and the mode typical for the deprotonated form at 1498 cm)1, indicating the protonation of a tyrosine residue with the reduction of the protein Also the m7¢a(CO) and d( COH) of tyrosine side...

Ngày tải lên: 21/02/2014, 15:20

... monitored at 420 nm All other experimental details were analogous to those used in CD spectroscopy Species DAHKam VIHN HL H2L H3L H4L 10. 52( 2) 18.05 (2) 24 . 32( 2) 27 .16(3) 7. 92( 2) 14.48 (2) 18.37(3) Table ... coordination (in agreement with previous observations [6,7]) A slight redshift of dd bands accompanied by a subtle decrease of delocalization of the unpaired d electron of the Cu(II) ion in the DAHKam ... and for related compounds The cases of highest and lowest afnities were selected from literature data The binding afnities for the model peptides are in the middle of the range of values for...

Ngày tải lên: 22/02/2014, 04:20

... ventricle at the end of systole Preload: The "load" imposed on the ventricle at the end of diastole Measures of preload include end-diastolic volume, end-diastolic pressure and end-diastolic wall ... period of time during which the mechanical properties of the heart are characterized by the EDPVR; 150 knowledge of the EDPVR allows one to specify, for the end of 125 diastole, EDP if EDV is ... Technically, it is defined as the reciprocal of the slope of the EDPVR ([dP/dV]-1) Colloquially, it is frequently used in describing the elevation of the EDPVR Stiffness (dV/dP) is the reciprocal of compliance...

Ngày tải lên: 22/02/2014, 09:20

... of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant avoredoxin Reduction titration of avoredoxin by NAD(P)H The reduction of ... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2 -P-AMP moiety is held in the groove between the ... avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin gene We determined the nucleotide...

Ngày tải lên: 07/03/2014, 02:20

... dependent fibril properties G Lodderstedt et al that has been described for monitoring fibril formation, e.g of the NM domain of the yeast prion protein Sup35p [29 ] and also for the detection of ... ANS fluorescence The increase in the ANS signal was reciprocal to the decrease in monomeric species (data not shown) and depended on the numbers of seeds added (Fig 4) Comparison of the fibrillation ... structure of the fibril and the b-strand arrangements remains to be determined Yet, knowledge of the structure and biophysical analysis of the fibrils alone will not suffice for understanding the disease...

Ngày tải lên: 07/03/2014, 11:20

... 24 36.9 24 79.0 25 21.5 20 80.6 21 23 4 32. 5 869.9 786.5 435.4 20 76.3 21 18.3 121 4.1 22 62. 4 23 04.4 20 22. 3 20 64.3 21 06.3 24 37.9 24 79.9 25 21.9 20 81 .2 2 123 .2 13–16 6– 12 1–5 49– 52 53–71 53–71 17 29 53–73 53–73 ... ID, identification number of peaks HPLC peak ID Measured mass (Da) Theoretical mass (Da) Peptidea 4 32. 5 869.1 786.5 435.1 20 75.5 21 17.9 121 4.7 22 61.6 23 03.5 20 22. 4 20 64.4 21 06.3 24 36.9 24 79.0 25 21.5 ... protein folds compatible with the SV-IV sequence PHD [33], JPRED [34], and PSIPRED [35] web services were used to predict the secondary structure The 3D model of the peptide corresponding to the segment...

Ngày tải lên: 07/03/2014, 14:20

... magnitude of the changes in Fe-His band frequencies and the redox potential values This is exemplied by the case of CCP and its mutants D2 35E, D2 35N and D2 35A [27 ] The H-bond between the proximal ... established for the wildtype protein (Fig 2) [20 ], while the blue-shift of the Soret and the changes in the visible region of the Ca-depleted and F 221 M spectra indicate a more or less marked presence of ... mediated exclusively on the reduced state of the enzyme We hypothesize that this residue imposes a degree of rigidity to the structure of the reduced state of class III peroxidases Results and...

Ngày tải lên: 07/03/2014, 21:20

... exchanger and of caveolin, a marker of lipid rafts To avoid possible detergent-induced artifacts that could either alter the distribution of the Na+/H+ exchanger or the constituency of the lipid rafts, ... aggregation of the protein because aggregates could often be seen on the top of the SDS/PAGE gels depending on the conditions of electrophoresis (Fig 2) The effect was not due to overexpression of the ... transfected with the NHE1 isoform of the Na+/H+ exchanger Membranes were fractionated as described in the Materials and methods and separated by SDS/PAGE Membrane fractions for A D and G were prepared...

Ngày tải lên: 08/03/2014, 16:20

... were deduced using the combined information of the intensities of Jab couplings from the 2D DQF-COSY spectrum and the NOE intensities of the HN-Hb and Ha-Hb cross peaks from the 2D and 3D NOESY ... only the best defined region (residues 9–41, Table 3) of the final ensemble of the 24 structures of RIIa D/ D shows that 82. 8% of residues are found in the most favored region, 15.8% in the additionally ... layer around the ˚ protein defined by a probe of 2. 0-A radius Finite difference focusing methods [22 ,23 ] were used in the calculations, with ˚ focusing grids of 2. 5, 1 .25 , 0.5, and 0 .25 A The parameter...

Ngày tải lên: 08/03/2014, 22:20

... resulted in a 2. 5-fold increase in specific GTPase activity and increased formation of GMP during hydrolysis Thermodynamics and stoichiometry of nucleotide binding We used ITC to investigate the thermodynamics ... Biophysical properties of hGBP5a ⁄ b and hGBP5ta M Wehner and C Herrmann hand, interact dynamically with the bound nucleotide, and usually bind nucleotides in the micromolar range In addition to these ... GTP-bound form does Similar to the monomeric species for the GDP-bound state of hGBP5ta, a recent study found that dynamin oligomers are repeatedly dissociated while GTP hydrolysis occurs [22 ] In...

Ngày tải lên: 15/03/2014, 10:20

... reagents] and separated by SDS/PAGE The appearance of a band in the different conditions used (native, reduced, reduced and denatured) on the different proteins (PfPDO and mutants) is evidence of the exposure ... PfPDO; (c) PfPDO (C35S); (d) PfPDO (C146S) (B) HPLC The disulfide bond-forming activity of PfPDO is monitored using the synthetic decapeptide NRCSQGSCWN and the oxidation of this dithiol peptide ... described by Hollecker et al [21 ], on the protein treated under different conditions (native, reduced, and reduced and denatured) (Table 1) By comparing the results obtained from the different...

Ngày tải lên: 16/03/2014, 16:20

... and the other at about 43 °C during the thermal-induced unfolding monitored by CD Previous investigations using CD of the thermal-induced unfolding of skeletal Tm from other organisms also identified ... dichroism parameters for the thermal-induced unfolding of wild type (WT) and mutant Tms The values are the mean ± standard deviation of at least three experiments Tm1 is the midpoint of the thermal ... thermodynamic parameters determined by DSC for the wild type (WT) and mutant Tms The uncertainties listed are the standard errors of the mean and included the uncertainty in the determination of protein concentrations...

Ngày tải lên: 16/03/2014, 18:20

... 3B) of the biopterin cofactors or dopamine the rate of phosphorylation was decreased, and at 20 0 lM of the ligands the inhibition was more pronounced for H4biopterin ( 26 %) and dopamine ( 26 %) ... both the reduced and the oxidized cofactor interact with the N-terminal autoregulatory sequence at Ser23 A close-up of this site of interaction (Fig 4A,B) shows that the O1Â and O2Â of the dihydroxypropyl ... note that the dihydroxypropyl side-chain is positioned slightly different in the two redox states of the cofactor and that the distances from Ser23O to O1Â and O2Â are slightly different (Table 1)...

Ngày tải lên: 17/03/2014, 09:20

... sequence [28 ] The underlined nucleotides refer to nucleotides that have been altered to generate an upstream NdeI site The bold ÔAÕ represents the first nucleotide of the otsA ORF DC 155, the downstream ... coupled assay, as an indirect measure of the amount of trehalose-P synthesized However, for most of the studies described here, the anthrone assay was used The substrate specificity of the recombinant ... assayed by the anthrone colorimetric method and the other set was assayed by the coupled enzyme assay where the amount of UDP produced was measured by its conversion of PEP to pyruvate and pyruvate...

Ngày tải lên: 17/03/2014, 10:20