... CTCTTGGGATCCTCCTCAGTGTTTAGCATGGTG CCTCTTCATCTGGCCGCACCACCTGACAACACG CCAGACCACCTGACAACACGGCAGCTTACTTAGTATTAGCTATAG GTTCCTCTATACACTAACCGCAAATACCCTTCCACACACCGG CCGCACGGGGGTGCATACGTGGACGGCACC GTACGTGGACGGCACCGCAGGAATTCTCTACAACAG ... Science Council of Republic of China Grant NSC 94 -23 11 -27 52- B-005-011-PAE and NSC96 -27 52- B-005009-PAE References The non-specific RNA binding of TGBp2 also raises the question of "how the non-specific ... property of TGBp2, which is responsible for the non-specific interaction between TGBp2 and viral RNA On the basis ofthe known topological propertiesof TGBp2 [9], we propose that the self-assembly of...
... W/(m K) 2. 681 2. 701 2. 610 2. 548 2. 678 2. 656 2. 646 Diffusivity α⋅106 m2/s 1 .24 1 1 .24 6 1 .26 3 1 .25 4 1 .24 9 1 .26 5 1 .25 3 Volumetric thermal capacity ρc⋅10-6 J/m3K 2. 16 2. 17 2. 18 2. 16 2. 20 2. 18 2. 18 Mean ... characteristics ofthe "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites ofthe "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... Researcher at theof Engines and Energy Institute ofthe Faculty of Engineering at the University of Bari from 1983 to 1987 Associate professor of Technical Plants at the Faculty of Engineering of the...
... mitosis Bioinformatics 22 , e158–e165 18 Fuss H, Dubitzky W, Downes CS & Kurth MJ (20 07) Deactivation of Src family kinases: hypothesis testing 4116 19 20 21 22 23 24 25 26 27 28 29 30 31 using a Monte ... transition from the Off state to On state (in the bistability domain) Regardless ofthe slope, the active Src fractions increase during the Off to On transition, whereas the value ofthe inactive ... conformation, the SH2 domain binds to pYi on the C-terminal tail, and the Src homology (SH3) domain binds to the linker between the SH2 and kinase domains at the back ofthe small lobe, preventing the...
... al [1] The medium contained (per L): 1. 32 g (NH4)2SO4, 0 .28 g KH2PO4, 0.07 g CaCl2.2H2O, 0. 02 g 0 .25 g MgSO4.7H2O, FeCl3.6H2O, 1.8 mg MnCl2.4H2O, 4.5 mg Na2B4O7.10H2O, 0 .22 mg ZnSO4.7H2O, 0.05 ... from that ofthe sample incubated in the absence of salts (data not shown) Also, EDTA completely abolished the stabilizing effect of Zn2+ When the enzyme was incubated with ZnCl2 and EDTA supplied ... abolished by the chelating agent EDTA However, the addition of Zn2+ did not affect the specific activity ofthe enzyme, and even high concentrations of EDTA (20 mm) could not decrease the activity of GdhA...
... uncouples or changes cooperativity in the binuclear center [20 ,22 ,28 ] The shifts ofthe titration curve upon addition of cyanide can be seen in Fig 2B (open circles) The theoretical Nernst fit yielded ... Em1 ¼ )0 .22 ± 0.01 V, Em2 ¼ 0.00 ± 0.01 V and Em3 ¼ 0.17 ± 0. 02 V (the values correspond to )0.0 12, 0 .20 8 and 0.378 V vs SHE¢, respectively) The potential at )22 0 mV can be attributed to the heme ... modes on the basis ofthe data presented here, in spite ofthe fact that bands in the difference spectra are observed in the region where the modes were attributed The vibrational modes of bound...
... mechanical properties by plotting the time course of change in the slope ofthe instantaneous PVR Above, we referred to the slope ofthe ESPVR as an elastance Similarly, we can refer to the slopes ofthe ... constant, but varies with atrial contraction and instantaneous atrial volume) The pressure ofthe point at the bottom right corner ofthe loop is the pressure in the ventricle at the end ofthe ... as the pressure ofthe left upper corner ofthe loop; the significance of this pressure will be discussed in detail below Moving to the bottom ofthe loop, we can reason that the pressure of the...
... b sheet with the b 12 ofthe other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity ofthe other monomer For the FeR dimer, the N-terminal ... with the FAD and NADH domains of BenC, which is the reductase component of benzoate dioxygenase reductase The crystal structure of BenC [27 ] shows that Cys307, which corresponds to Cys4 12 ofthe ... Lopez R et al (20 07) Clustal W and Clustal X version 2. 0 Bioinformatics 23 , 29 4 729 48 FEBS Journal 27 6 (20 09) 48404853 ê 20 09 The Authors Journal compilation ê 20 09 FEBS N Shibata et al Supporting...
... N-terminal domain of PABPN1 consisting of amino acids 1– 125 ofthe wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation propertiesofthe most extreme ... Simonelig M (20 06) A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1 EMBO J 25 , 22 53 22 62 Hoyt MA, Zich J, Takeuchi J, Zhang M, Govaerts C & Coffino P (20 06) ... using the nanoscope analysis software Chemical stability of fibrils The chemical stability of fibrils was tested after the fibrillation process was completed (no further rise of ANS signals) The sample...
... F1 72, F 229 , G168, D230, I 228 and is directly constrained by the carbonyl oxygen of G168 Inclusion of F 221 obscures the local structure in the proximity of T171 and for clarity is omitted Table ... reduction ofthe catalytic activity if they have a direct impact on the disposition ofthe catalytically important His 42 and Arg38 residues [29 ,30] However, although the Fe-Im bands of many distal mutants ... and specic to the reduced state ofthe enzyme The Fe(II) state ofthe enzyme has features in common with both the Phe 221 Met mutant and Ca-depleted proteins whilst the Fe(III) state is essentially...
... acids ofthe protein The immunoprecipitate was then probed with the anti-NHE1 monoclonal antibody Ó FEBS 20 02 4890 B L Bullis et al (Eur J Biochem 26 9) A 0% βME 1 .25 % βME 3% βME 0% βME B 2% SDS 2% ... amount at the 105–110 kDa size The amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top ofthe gels The amount of aggregate present at the ... concentrations ofthe fractions, together with the relative amount of Na+/H+ exchanger present in the supernatant and the pellet [19], it was found that over 80% ofthe Na+/H+ exchanger remained in the...
... observed 20 46 D Morikis et al (Eur J Biochem 26 9) Ó FEBS 20 02 Fig The hydrophobic and electrostatic character of RIa D/D (A) A backbone representation of residues 9–41 ofthe ensemble ofthe 24 lowest ... contributes to the stability and packing ofthe dimer Parallel packing ofthe aromatic side chains Tyr16, Phe31, Tyr35 and Phe36 and contacts involving the side chains of Leu 12, Val20, Leu28, Val29, Val33, ... shows the van der Waals model ofthe top face ofthe best structure of RIIa D/D in the region )1 to 44 This view is generated by rotating the orientation of RIIa D/D of Fig 4A by 90° about the...
... displays of status They often express agreement with a partner’s suggestions And when they have ideas of their own, they’ll put them in the form of questions, such as “I’ll be the teacher, okay?” Their ... created The boys pushed the girls around, refused to take turns, and would ignore a girl’s request to stop or give the toy back By the end ofthe morning, Leila had retreated to the other end ofthe ... The Female Brain is one ofthe most-talked-about booksofthe year “I’ve found I can change the conversation at any social gathering by mentioning Louann Brizendine’s book, The Female...
... : (25 0 mM); (25 0 mM) : 0; : (each 125 mM); (187.5 mM) : ( 62. 5 mM); (22 5 mM) : (25 mM) At the end ofthe incubation, 40 lL ofthe mixture was added to each ofthe five solutions; these were then ... assayed at 1 .2 lM for all the proteins Both the wild-type PfPDO and the mutant C35S were active in the insulin reductase assay [23 ], whereas the activity ofthe mutant C146S and the double mutant was ... coefficient of 5600 M)1Æcm)1 at 27 8 nm The oxidized state ofthe peptide was generated by incubating the peptide at a concentration of 50 lM in 0 .2 M Tris/HCl, pH 8.4, at 20 °C for 15 h The reduced state...
... shows the fitting ofthe DSC scan for wild type Tm obtained using three endotherms The Tms ofthe wild type and mutant endotherms are shown in Table It is evident from the data that the unfolding of ... stability ofthe mutant Tms The mutations did not affect the structure ofthe protein as there was no significant alteration in the function or in the amount ofthe secondary structure However, the mutations ... 60 50 0.00 0 .25 0.50 0.75 1.00 1 .25 1.50 1.75 2. 00 Tropomyosin (µΜ) A WT (-[θ ]22 2 ) d-[θ ]22 2 /dT 35000 2+ 30000 -1 -[θ ]22 2 (deg.cm dmol ) 25 000 Fig Inhibition of actomyosin S1 Mg ATPase activity...
... in the two redox states ofthe cofactor and that the distances from Ser23O to O1Â and O2Â are slightly different (Table 1) because ofthe different positions and hybridizations ofthe C6 atom of ... explaining the redox state dependent regulatory propertiesofthe pterin cofactor Moreover, the superposition ofthe ternary structure [21 ] revealed a similar orientation ofthe pterin cofactor as in the ... Biochem 27 0) 987 Table Comparison of distances (A) ofthe superposition ofthe crystal structure of ligand-free dimeric rPAH (PDB id code 1PHZ), which contains both the regulatory and the catalytic...