... Plants at the Faculty of Engineering ofthe Polytechnic of Turin from 1987 to november 1991 Full Professor ofthe ISS ING-IND 11 - Environmental Technical Physics, always at the university ofthe Basilicata ... if the present structures are characterized with local materials, namely, “Tufo of Matera” for the structures ofthe "Sassi of Matera," and “Stone of Fasano” for the structures ofthe "Trulli of ... and the knowledge ofthe temperature coefficient ofthe material of which it is made A single measure allows for the determination of diffusivity, thermal conductivity, and specific heat of the...
... QoL and the core symptoms The influence ofthe QoL ofthe parents or the parents’ diseases (such as ADHD) could not be assessed because these data were not obtained Conclusions The strength of this ... smaller The RA and the PA sub-domains had lower correlations with their domains than most ofthe other domains at baseline, at endpoint, and Psychometric propertiesofthe CHIP-CE also for the change ... use the sub-domains rather than the domains ofthe CHIPCE when evaluating ADHD patients This is supported by the factor analysis based on the sub-domains and the correlation analysis ofthe sub-domains,...
... by presenting the pulse of Src activity in the plane ofthe inactive and active fractions, si and s1 If the duration ofthe stimulus exceeds the critical value, the trajectory in the (si, s1) ... transition from the Off state to On state (in the bistability domain) Regardless ofthe slope, the active Src fractions increase during the Off to On transition, whereas the value ofthe inactive ... all steady states ofthe Src cycle When there are three intersections, the steady state O1 at the lower branch ofthe Z-shaped curve (i.e low Src activity) and the state O3 at the upper branch...
... containing the structural zinc at the mouth ofthe cleft and thus exposed to the solvent [8] Gradual depletion ofthe enzyme first ofthe structural and then ofthe catalytic zinc has also been ... rate under the conditions present in the cytoplasm of Picrophilus The kinetics of NADPH degradation was followed by measuring the rate of decrease of its absorbance at 340 nm over the pH range ... abolished by the chelating agent EDTA However, the addition of Zn2+ did not affect the specific activity ofthe enzyme, and even high concentrations of EDTA (20 mm) could not decrease the activity of GdhA...
... cooperativity ofthe other redox centers In order to discriminate the contributions ofthe cofactors, inhibitors uncoupling or changing the cooperativity can be used Addition of cyanide strongly shifts the ... spectra and the deconvolution ofthe amide-I region A particular problem ofthe assignment in the difference spectra is the superposition of signals from different constituents ofthe oxidase, ... modes on the basis ofthe data presented here, in spite ofthe fact that bands in the difference spectra are observed in the region where the modes were attributed The vibrational modes of bound...
... represent the native HSA sequence and Val-Ile-His-Asn (VIHN), the N-terminal peptide of another blood serum protein, des-angiotensinogen [11] The structure ofthe Ni(II) complex ofthe latter ... below) The comparison of CD spectra of complexes also points toward slight differences in the conformation ofthe chelate rings The characteristic alternate pattern ofthe dd bands in the CD ... complexes of GGH, where the a carbon ofthe His residue is the sole source of chirality [10] However, while positions ofthe component dd bands and of CT transitions are relatively constant, their...
... as the pressure ofthe left upper corner ofthe loop; the significance of this pressure will be discussed in detail below Moving to the bottom ofthe loop, we can reason that the pressure ofthe ... instantaneous atrial volume) The pressure ofthe point at the bottom right corner ofthe loop is the pressure in the ventricle at the end ofthe cardiac cycle and is called the end-diastolic pressure ... mechanical properties by plotting the time course of change in the slope ofthe instantaneous PVR Above, we referred to the slope ofthe ESPVR as an elastance Similarly, we can refer to the slopes of the...
... b sheet with the b12 ofthe other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity ofthe other monomer For the FeR dimer, the N-terminal ... Resolution ofthe structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side ofthe isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... in the binding ofthe phosphate group(s) of FMN or FAD Lys92 forms a salt bridge with the O3P of FMN A salt bridge that involves avin species has not been reported in the structures ofthe other...
... domain of PABPN1 consisting of amino acids 1–125 ofthe wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation propertiesofthe most extreme disease-associated ... that the increase in ANS fluorescence correlates with the increase in fibrillar species at the expense of monomeric protein According to the hypothesis that seeded fibril formation in the case ofthe ... using the nanoscope analysis software Chemical stability of fibrils The chemical stability of fibrils was tested after the fibrillation process was completed (no further rise of ANS signals) The sample...
... acetylation at one ofthe three residues, because the signal ofthe triacetylated species was less intense than that ofthe diacetylated species The MS/MS Ó FEBS 2003 Structural propertiesof SV-IV (Eur ... the total number of charges measured by electrospray MS Theoretical mass is the mass calculated on the basis ofthe protein amino-acid sequence The relative abundance refers to the ratio ofthe ... the mass increase of 42 mass units The relative level of acetylation of a peptide was estimated on the basis ofthe intensity ratio ofthe native and acetylated species From the data summarized...
... way to the wild type [16,17] The pKa for the alkaline transition being similar to that ofthe wild type, % 11. 1 [18] Finally, comparison ofthe X-ray structures ofthe oxidized forms ofthe native ... disposition ofthe proximal His and Asp residues, affecting the imidazolate character ofthe His, but also by the formation of a LS species The latter indicating the probable binding of His42 to the ... impact on thepropertiesof His170 The strength ofthe hydrogen bond between the proximal His and Asp residues, and thus the imidazolate character ofthe His is expected to modulate not only the strength...
... domain-specific thesaurus In our case, since the books can cover several domains, the construction and use of domain-specific thesauruses is not plausible, as the advantage of such resources is offset by the ... reduce the size ofthe data set, possibly eliminating the training instances that will have the least negative effect on the usability ofthe data set The first step to reduce the size ofthe data ... permutation ofthe composing words for each index entry, looks up the frequency of that permutation in the book, and then chooses the one with the highest frequency as the correct reconstruction of the...
... amount at the 105 110 kDa size The amount of 105 110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top ofthe gels The amount of aggregate present at the ... b-cyclodextrin-treated cells ofthe Na+/H+ exchanger, biochemical characterization ofthepropertiesofthe full length protein has proven difficult because of its low abundance and because ofthe difficulty ... either alter the distribution ofthe Na+/H+ exchanger or the constituency ofthe lipid rafts, we used an established detergent-free lipid raft isolation procedure for some of our studies [11] ...
... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt ofthe N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position ofthe ... shows the van der Waals model ofthe top face ofthe best structure of RIIa D/D in the region )1 to 44 This view is generated by rotating the orientation of RIIa D/D of Fig 4A by 90° about the ... hydrophobic interface ofthe two monomers (Fig 6B) A further rotation ofthe orientation of RIIa D/D of Fig 4B by 180° about the x-axis shows the bottom face of RIIa D/D (Fig 4C) There is some hydrophobic...
... true in many other fields, as well For example: • 20% ofthe input creates 80% ofthe result • 20% ofthe workers produce 80% ofthe result • 20% ofthe customers create 80% ofthe revenue • ... very closely to the sound ofthe word he is learning The problem here is that the vowel [aun] ofthe English word and the Chinese equivalent are not exactly the same, so when he uses the Chinese ... verbally, the speaker has to coordinate various parts of his body from the brain to the tongue, the mouth, the lips, the breath and so on You may not be aware of this complexity with your mother tongue...
... mass (Mapp) in the GDP-bound form of approximately 70 kDa, most likely due to an elongated shape ofthe protein The same behavior is observed in the presence of GDPÆAlFx Because ofthe unexpected ... for the lack of GMP formation (see Fig S2) The similar hydrolytic activities ofthe a ⁄ b and C-terminally truncated forms of hGBP5 are in contrast to that ofthe analogous deletion mutant of ... conserved in the primary structure ofthe GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner Sequence alignments ofthe large GTP-binding domains of hGBP1,...
... the presence ofthe nucleotide These experiments indicate that the binding and/or hydrolysis of ATP not have any effect on the conformation of PfPDO, possibly because ofthe localization ofthe ... participation of both N-terminal and C-terminal active sites Refolding ofthe scrambled RNase in the presence of PDI was used as a positive control, and the absence ofthe recovery ofthe RNase activity ... using PDI However, the temperature ofthe assay, which is limited by the stability ofthe protein substrate RNase, is very far from the optimal growth temperature ofthe hyperthermopilic micro-organism...
... stability ofthe mutant Tms The mutations did not affect the structure ofthe protein as there was no significant alteration in the function or in the amount ofthe secondary structure However, the ... probe Thus, the analysis ofthe melting profile of human Tm was enhanced by the use of different probes The mutations affect the stability ofthe protein Heller et al ([19] and references therein) ... in the unfolding of chicken Tm monitored by CD and suggested that the lower Tm (Tm1) reflected the stability ofthe C-terminus and the higher Tm (Tm2) reflected that ofthe N-terminus These authors...
... features of its hysteretic properties Thus, the structures ofthe binary and ternary complexes of hPAH have provided a detailed picture ofthe protein contacts involved in the binding of biopterin cofactor ... both the regulatory and the catalytic domains (residues 1429), were performed to demonstrate the interactions ofthe dihydroxypropyl side-chain ofthe pterin cofactors with the N-terminal autoregulatory ... both the reduced and the oxidized cofactor interact with the N-terminal autoregulatory sequence at Ser23 A close-up of this site of interaction (Fig 4A,B) shows that the O1Â and O2Â ofthe dihydroxypropyl...
... for the four substrates shown in the reaction It seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another ... emerged in the region suggesting a molecular weight of 220 kDa (Fig 3) These data suggest that the active TPS probably exists as a tetramer Propertiesofthe recombinant TPS The activity ofthe purified ... compared to the amount of NAD+ produced (nmol) in the coupled assay, as an indirect measure ofthe amount of trehalose-P synthesized However, for most ofthe studies described here, the anthrone...