... thermochemistry), the steric interactions that govern these preferences are likely to be present to some extent in the transition states More importantly, the conformation of the terminal unit of the ... a better measure of their effective steric bulk The present results thus suggest the rotational barriers are likely to be significant It is interesting to note that in Figure 5, apart from the ... studies need only take into account the stereochemistry of the penultimate unit of the polymer chain into account when predicting the stereochemistry; further ab initio testing of this result...
Ngày tải lên: 14/03/2014, 09:20
... Believing that the contraction of the sub-unit could be pulling the fiber and causing microbends, ADC set out to test the theory Choosing a sub-unit that was exhibiting high attenuation loss at low temperature, ... section, the attenuation disappeared - thus isolating the problem to the fiber sub-unit alone Although this, in itself, provided a potential field solution–stripping the entire length of sub-unit material ... both labor-intensive and risky to the fiber As a result, ADC set out to establish a craftfriendly procedure that would simplify the solution to the problem Using a typical fiber cutting tool to...
Ngày tải lên: 10/12/2013, 20:15
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... nucleophile attack by the Oc of the catalytic serine, while the proton is shuttled to the catalytic histidine This results in the formation of a tetrahedral transitionstate intermediate, the negative ... bkcat activity required protonation of two amino acids This biphasic titration accentuates the fact that the intermediate, singly protonated species is active That is to say, complete protonation ... shift supports the assumption that the observed pKa is related to His438 because it is consistent with the fact that the electrostatic stabilizing effect of the E197 side chain on the protonated...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... weight) At time intervals, aliquots were taken from the reaction mixture, and the proteolysis was stopped by adding to the mixture the inhibitor pepstatin (enzyme ⁄ inhibitor molar ratio of : 5) The ... exposure (a) to water of Tyr residues in proteins can be estimated by second-derivative UV spectroscopy [31] This method takes advantage of the fact that the peak-to-trough distances in the 280– ... of the Hill plot indicates absence of cooperativity, which is consistent with the monomeric structure of both proteins It is of note that even at pH 4.7, Ngb retains the ability to bind 0.8 0.8...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc
... substantially in their sequences at these locations, consistent with the possibility that the functions of the single c-tubulin found in most organisms may be partitioned between the two protistan ... shown that activation of the heatshock response in the ciliate T thermophila requires GATA motifs, heat-shock transcription elements, and their cognate transcription factors [39] Taken together, ... nucleotides shorter than that of c -T1 but, otherwise, these two sequences were quite similar We have not yet investigated the role of message degradation with respect to the control of c-tubulin transcript...
Ngày tải lên: 07/03/2014, 04:20
Changes in the Economic Value of Variable Generation at High Penetration Levels: A Pilot Case Study of California pot
... AS The costs that are attributed to VG reflect the assumption that AS requirements change in proportion to the DA schedule for VG The amount of AS added to compensate for the additional short-term ... have the incentive to not participate in the DA market (or bid a very high cost so that they receive a DA schedule that has them not generate) but then make the generation available in the RT to ... conventional generation that enters the market is able to cover its annualized fixed cost of investment In other words, the short-run pro t of incumbent generation that stays in the market must exceed...
Ngày tải lên: 08/03/2014, 06:20
Maximum reproductive rate of fish at low population sizes potx
... catch rates to estimate the numbers at age in the final year and to reconstruct previous numbers at age under the assumption that catch at age is known without error and that natural mortality ... reproductive rate (the uncorrected value) with the expectation, where the median and expectation are taken with respect to the distribution of the random effects 2411 (Fig 7) The corrected estimates ... of the database used here The ML estimators that we have used to estimate the underlying distribution of annual reproductive rates may result in estimates that are less “heavy tailed” than they...
Ngày tải lên: 14/03/2014, 16:20
Crop uptake and extractability of cadmium in soils naturally high in metals at different ph levels
Ngày tải lên: 15/03/2014, 23:22
TiO2nanotubes and CNT–TiO2hybrid materials for the photocatalytic oxidation ofpropene at low concentration
... TiO2 Thus, the TiO2-NT2 photocatalyst, which contains 72% of anatase and 28% of rutile, is the best one The other is the composition, especially the carbon content Thus, the CNT–TiO2 photocatalyst, ... heat treatment in air at 520 8C Sample TiO2-NT3 was prepared by heat treatment in Ar at 700 8C followed by a heat treatment in air at 520 8C XRD analysis showed that the heat treatment in air at ... with the purpose of studying the effect of the crystalline composition (anatase/rutile) in the catalytic activity In addition, the effect of the presence of the carbon nanotubes in the CNT–TiO2...
Ngày tải lên: 20/03/2014, 13:11
Báo cáo Y học: Characterization of a partially folded intermediate of stem bromelain at low pH ppt
... with a concomitant decrease in the ¯uores- cence intensity These observations suggest that the protein at pH 2.0 is present in a conformational state that is different from the native state at ... compared to the protein at neutral pH The properties of the pH 2.0 state proteins are intermediate between those in the native state and molten globule state and justify its occurrence on the native ... than that of the possible molten globule state at pH 0.8 but greater than that of the native state Acrylamide quenching data clearly show that stem bromelain at pH 2.0 is in an unfolded state as...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo khoa học: ATPase activity of RecD is essential for growth of the Antarctic Pseudomonas syringae Lz4W at low temperature potx
... activity (data not shown) This is surprising, considering the fact that the protein displayed approximately 30% ATPase activity at °C This suggests that the DNA strand separation assay in vitro ... interesting questions about the contribution of these two enzymatic activities to RecD function during growth at low temperature While it is impossible to directly assess these enzymatic activities of ... ATPase activity with 3¢ overhangs and blunt-ended DNA (Fig 2A) No detectable intrinsic ATPase activity was associated with the protein To compare the ATP-hydrolyzing activities of the mutant...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: The histidine-phosphocarrier protein of Streptomyces coelicolor folds by a partially folded species at low pH ppt
... from a state with residual secondary structure, but no tertiary-like structure, to a conformation with native-like secondary and tertiary structure (Fig 1) This acquisition of tertiary structure ... observed at lower pH values Although it is difficult to rationalize a native-like tertiary structure around the tryptophan without the acquisition of a native-like compactness, it could be that the ... buffer The continuous lines represent the fitting of the experimental data to a two-state reversible model binding did not show any sigmoidal titration (data not shown), suggesting that no intermediate...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo hóa học: " Catalytic ozone oxidation of benzene at low temperature over MnOx/Al-SBA-16 catalyst" pdf
... et al Nanoscale Research Letters 2012, 7:14 http://www.nanoscalereslett.com/content/7/1/14 catalytic activity of Al-SBA-16-MA15% was higher than that of Al-SBA-16-MN15% It was shown that the type ... Park et al Nanoscale Research Letters 2012, 7:14 http://www.nanoscalereslett.com/content/7/1/14 (Mn nitrate), to investigate the effect of Mn precursors on the catalytic ozone oxidation of ... small (200) reflections This result indicates that the cubic mesostructure was not destructed by the incorporation of alumina on the silica framework It is shown in Figure that the Mn/ Al-SBA-16...
Ngày tải lên: 20/06/2014, 23:20
Báo cáo hóa học: " A Versatile Route for the Synthesis of Nickel Oxide Nanostructures Without Organics at Low Temperature" doc
... and the state of water at elevated temperatures is different from that at room temperature The solubility and the reactivity of the reactants also change at high pressures and high temperatures, ... out and cooled to room temperature naturally The resulting reaction mixture was centrifuged to reclaim the precipitated product The final product was filtered, washed with deionized water and ethanol ... simplicity and practicability of synthesis techniques When developing a synthesis method for generation of nanostructures, the most important issue that one needs to consider is the simultaneous control...
Ngày tải lên: 22/06/2014, 01:20
Báo cáo hóa học: " Research Article How to Solve the Problem of Bad Performance of Cooperative Protocols at Low SNR Charlotte Hucher, Ghaya Rekaya-Ben Othman, and Jean-Claude Belfiore" potx
... estimates its own source-relay link and transmits a single bit to the destination indicating whether it is in outage or not Then, the steps are the same as for the adaptive AF: the destination ... it is not in outage, then three different transmission schemes can be considered: 6.2 Implementation constraints As in the adaptive AF strategy, it is the destination who has to select the best ... one with the greatest instantaneous capacity 8.1 PERFORMANCE OF THE ADAPTIVE DF STRATEGY Outage probability The outage probability of the adaptive DF protocols can be proven to be lower than the...
Ngày tải lên: 22/06/2014, 19:20
báo cáo khoa học: " Light has a specific role in modulating Arabidopsis gene expression at low temperature" potx
... assimilation before the treatments, plants were transferred after hours of the beginning of light period directly from growth temperature to the low temperature treatment The cold treatments were ... in vitro translated proteins, mM L-methionine was used Five double stranded oligonucleotide probes were made: DREB 5'tgactaCCGAcatgagttcc3', ABF 5'ccttgtccacGTGTatc atc3', DOF 5'atcttatatAAAGcaccatt3', ... growth The relative humidity was controlled during growth and low temperature treatments and kept constantly at 60% Cold treatments for hours were performed at 3°C (measured from the bottom of the...
Ngày tải lên: 12/08/2014, 05:20
báo cáo khoa học: " Spatial distribution of transcript changes in the maize primary root elongation zone at low water potential" docx
... plant reproductive success and survival Indeed, the adaptation of roots to counteract a limiting water supply is highlighted by the fact that root growth is often less sensitive to water deficit ... groups: those that were part of the stress response that brought about early root cell maturation, and those that were part of maturation itself Region contained a greater number of differentially ... different regions of the root growth zone 48 h after stress imposition when the root elongation rate was at steady state [4,6] Most striking was the complete maintenance of longitudinal expansion rate...
Ngày tải lên: 12/08/2014, 05:20
Báo cáo khoa học: "Hepatic Cobalt and Copper Levels in Lambs in Norway" docx
... cobalt-containing supplement This may indicate that cobalt supplementation was still not much used outside the south-west at the time these data were collected Cobalt supplements have been shown to ... unknown to the laboratory, but most probably contained cobalt The pasture histories of the flocks are indicated in Table 2, with capital letters indicating the main summer pasture Flocks with capital ... or after their period in the mountains Twenty-one flocks had grazed on uncultivated lowland pastures, but not in the mountains Forty-eight of the 50 flocks that had grazed on uncultivated mountain...
Ngày tải lên: 12/08/2014, 15:20
Báo cáo y học: " Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels" doc
... 37°C RTd PBS a The aim of this step is to eliminate proteins within the section which could otherwise interfere with binding of the probe to the target DNA b The aim of this step is to eliminate ... 3:38 eliminate RNA sequences including those that are homologous to the probe, NaOH treatment to denature the DNA present in the sections, and heat treatment to denature the double-stranded DNA ... The nature and mechanisms of this intranuclear transport remain entirely to be characterized Competing interests The author(s) declare that they have no competing interests Authors' contributions...
Ngày tải lên: 13/08/2014, 09:20
Báo cáo sinh học: "Genotype by environment interaction for adult body weights of shrimp Penaeus vannamei when grown at low and high densities" ppt
... identity e 546 A.M Ibarra, T. R Famula matrix and r2 is the unknown residual variance It is important to note that, e because of the mating system, g may contain effects beyond the additive genetic ... affect the average expression of traits, the amount of genetic and environmental variance of the traits, and consequently the heritability of the traits [9] Whereas a change in heritabilities ... it at high densities However, before selection for BW is practiced in this type of environment, a further understanding of the effects on genetic correlations of BW with other traits within this...
Ngày tải lên: 14/08/2014, 13:21