synthesis purification and characterization

... sequencing of our FIII-3a and FIII-3b proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles ... from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624), with a serine protease ... purification and characterization of xylanasefrom Staphylococcus sp SG-13 and its application in biobleaching of kraft pulp J Appl Microbiol 88(2):325–34 Gupta GS, Singh GP (1983) Isolation and...

Ngày tải lên: 20/06/2014, 23:20

... starting from the raw materials selection, synthesis and characterization of the as-synthesized and calcined nano-TiO2 powder, to powder consolidation and characterization of the sintered structures ... Materials and Aerospace Engineering (MMAE), Advanced Materials Processing and Analysis Center (AMPAC) and UCF for their financial and experimental support Moreover, I would like to thank my labmates and ... floor and walls of operating rooms, silicone rubber for medical catheters and hospital garments and uniforms Others Public rest rooms, bathrooms and rat breeding rooms Air cleaning Water purification...

Ngày tải lên: 02/07/2014, 12:57

... rD ot eud era sknaht luftraeH stnemgdelwonkcA nietorp siht fo noisserpxe ortiv ni dna ygolonhcet AND tnanibmocer fo noitacilppa eht ot gnidael ,erudecorp noitalosi ANRm na fo esu eht etatilicaf...

Ngày tải lên: 07/08/2014, 20:23

... Expression and characterization of the UL31 protein from duck enteritis virus Virol J 2009, 6:1 doi:10.1186/1743-422X-7-241 Cite this article as: Zhang et al.: Production, purification and characterization ... weight markers Lane and Lane respectively represented the uninduced and induced BL21 bacteria within pET-32b(+) plasmid; Lane and Lane respectively represented the uninduced and induced BL21 bacteria ... Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 represented bicolor prestained protein markers A Purification...

Ngày tải lên: 12/08/2014, 01:21

... surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification of LSV CP 15% SDS-PAGE ... understanding of its role in cell attachment and LSV tropism This investigation to LSV should provide some specific antibodies and aid to development a detection system for LSV diagnostics and epidemiologic ... with the antibody By bleeding and separation of antiserum and DEAE cellulose column purification, anti-LSV CP IgG (ALCP) was isolated A 34.5 kDa specific blotting band was detected at the corresponding...

Ngày tải lên: 12/08/2014, 04:21

... phytase: purification and characterization Biotechnol 19:508-520 Ullah, A H J and Gibson, D M 1987 Extracellular phytase (E.C 3.1.3.8) from Aspergillus ficuum NRRL 3135: Purification and characterization ... phytase from Lilium longiflorum: PuriWcation and structural characterization Protein Expression and Purification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of ... this research, the thesis on "Purification and characterizations of phytase enzyme from Aspergillus niger" has been performed with the aim to obtain purifed phytase and some its valuable characteristics...

Ngày tải lên: 06/10/2015, 13:05

... binding and selective alkylation leading to a depletion of mtDNA in intact cells (Fig 1) Here we report the synthesis and characterization of a novel mitochondria-targeted alkylating reagent and ... liver mitochondria (40 mg protein) and incubated with 500 lM mitoDC-81 for h and then cut with ClaI (C and D) DNA was separated by electrophoresis on an agarose gel and the ethidium bromide fluorescence ... bromide gel and the fluorescence recorded (A and C) The DNA was then transferred to a nylon membrane and TPP moieties covalently attached to the DNA were visualized using anti-TPP serum (B and D) The...

Ngày tải lên: 20/02/2014, 11:20

... parameters and transport characteristics of Bip-[3H]Pro Results and Discussion Synthesis, chemical characterization and stability of Bip-Pro Figure shows the structure (Fig 1A) and the synthesis ... Ala-4-nitroanilide and Lys(4-nitrobenzyloxycarbonyl)-Val were synthesized according to peptide synthesis standard procedures [18,29] All other chemicals were of analytical grade Synthesis of Bip-Pro and Bip-[3H]Pro ... existence of two conformations Fig Structure and synthesis of Bip-Pro (A) Bip-Pro structure (B) Bip-Pro synthesis: mixed anhydride (MA) method (C) Synthesis of Bip-[3H]Pro EDC, N-ethyl-N¢-(3-dimethyl...

Ngày tải lên: 07/03/2014, 05:20

... experimental chamber and it was reacted with hydrogen radicals SiNWs were synthesized for 20 at 400 °C Detailed other synthesis conditions are summarized in Table The optical and morphological characteristics ... diameters of SiNWs on the bottom and top were approximately 60 nm and thinner than 10 nm, respectively Their lengths extended up to ~ 1.5 μm Moreover, the SiNWs were tapered and catalysts remained on ... the typical dark and light I–V curve of the SiNWs solar cell Clear rectifying behavior and power generation was distinguished in the cm2 SiNWs solar cell The short circuit current and open circuit...

Ngày tải lên: 16/03/2014, 15:12

... deposition Microstructural characterization Scanning and transmission electron microscopy (SEM and TEM) have been carried out in order to determine the degree of homogeneity and crystalline arrangement ... of the nanowire lateral sides and apex Electron diffraction (ED) and analysis of zero-order and higher-order Laue-zones allows precise determination of unit cell and space group Incoherent imaging ... exceeds several microns and the width is smaller than 100 nm As shown in Fig 2a, the length and width of the nanowire measure 25.3 ␮m and about 50 nm, respectively The length and flexibility allows...

Ngày tải lên: 16/03/2014, 15:25

... both hydrophobic forces and hydrogen bonding between Tyr35 (dyad) and the aromatic cluster consisting of Trp344, Trp345* and Tyr355* (Fig 7B, legend), and between Ile28 and Val361 The Lys26 (dyad) ... and the filtrate was precipitated and washed twice with cold diethyl ether After centrifugation at 2800 g for 12 min, the supernatant was discarded and the crude peptide was dissolved in H2O and ... apamin and was shown to be less than 8% of the total binding Electrophysiology Oocyte preparation Xenopus laevis oocytes (stages V and VI) were recovered and prepared for cRNA injection and electrophysiological...

Ngày tải lên: 17/03/2014, 10:20

... reaction of bulky rutile powders and 10 M NaOH solution without using any templates and catalysts The inner and outer diameters of the nanotubes were approximately and nm, respectively The amount ... for 96 (c) and 144 h (d), the peaks at 250 and 620 cm−1 had almost disappeared and new peaks were observed near 290 and 660–760 cm−1 The peaks at 290 and 660–760 cm−1 might be suggested to be ... (c) and 144 h (d–f) J Yu, H Yu / Materials Chemistry and Physics 100 (2006) 507–512 accelerating voltage and the applied current were 40 and 40 mA, respectively X-ray photoelectron spectroscopy...

Ngày tải lên: 19/03/2014, 16:48

... filtered, washed with distilled water and absolute ethanol, and finally dried in vacuum at 50 ◦ C for h 2.2 Characterization The rodlike ␣-FeOOH (goethite) and porous fusiform ␣-Fe2 O3 (hematite) ... and O, and no contamination elements are detected The atomic ratio of Fe and O matched their stoichiometries quite well Associating all those results, the whole reaction to form the ␣-FeOOH and ... 24 h and indicate the large quantity and good uniformity ␣-Fe2 O3 nanocrystallines were achieved using this approach These fusiform ␣-Fe2 O3 nanocrystallines have a mean diameter of 60 nm and...

Ngày tải lên: 19/03/2014, 16:48

... , 754 cm−1 , and 810 cm−1 can be observed According to the lit- erature [23,24], these bands can be assigned to the fundamental modes of crystalline h-WO3 The bands at 754 cm−1 and 810 cm−1 ... mV/s, 500 mV/s, and 1000 mV/s during the 10th cycles ing mode of the bridging oxygen The band at 435 cm−1 can be attributed to the characteristic band of crystalline WO3 [23] Broadened and slightly ... confirmed by XRD and TEM analyses SEM and TEM images revealed self-assembled nanowire bundles and nanorods that formed urchin-like structures The shapes of the hWO3 nanowire bundles and urchin-like...

Ngày tải lên: 19/03/2014, 16:48

... Hydrothermal synthesis of ultra®ne Fe2O3 and Fe3O4 powders, Mat Res Bull 33 (1998) 841 [7] D Barb, L Diamandescu, D Mihaila-Tarabasanu, A Rusi, Romanian Patent Osim 86 979, 18 December 1984 [8] L Diamandescu, ... ®ltered and then oxidised by drying at 110 C in air F Another path in the hematite synthesis was the direct hydrothermal treatment of an homogeneous mixtures of ferric nitrate (1 M) and urea ... into ammonia and carbon dioxide, acting as precipitation agent In all cases after hydrothermal treatment, the powders were ®ltered, washed with distilled water, dried at 110 C in air and then investigated...

Ngày tải lên: 19/03/2014, 16:48

... substrate is clean and nothing is observed on its surface, and TiO2 nanorods grow radially on the unmodified substrate and form many papillae in a random pattern, as illustrated in Fig 1b and c The FE-SEM ... 8C and at a hydrothermal growth temperature of 160 8C and growth time of h (a) PT-substrate; (b) top view of TNAs; (c) side view of TNAs the nanorods have a diameter between 15 and 25 nm and ... electron microscope (TEM, PHILIPS-FEI TECNAI20) and high-resolution TEM (HRTEM, JEM-2010) were used to further elucidate the microstructure and phase characterization X-ray diffraction (XRD) analysis...

Ngày tải lên: 19/03/2014, 16:48

... bands at 3435 and 1635 cmÀ1, which correspond to the presence of the OH group and water, absorbed on the surface of the TiO2 samples [23–25] In addition, for samples S2–S4, two bands at 2860 and ... 8, 16 and 24 h, respectively, and (d) TiO2 nanotubes (sample S2); (e) HRTEM image of the sample S2 and the inset shows the enlarged picture of the tube wall; (f) HRTEM image of sample S1 and the ... nanorods, nanowires and nanotubes with different diameters and lengths were formed To understand the formation of the different 1D nanostructures controlled experiments were carried out and the products...

Ngày tải lên: 20/03/2014, 13:08

... Advanced chemical methods for soil and clay mineral research Dordrecht: Reidel; 1980 [40] Jeevananda T, Siddaramaiah S, Seetharamu S, Saravanan S, D’Souza L Synthesis and characterization of poly (aniline-co-acrylonitrile) ... AgNO3 and pyrrole in the presence of poly(vinylpyrrolidone) J Phys Chem B 2005;109:18283–8 [37] Sahoo NG, Jung YC, So HH, Cho JW Polypyrrole coated carbon nanotubes: synthesis, characterization, and ... the peak at 1040 cmÀ1 is attributed to C–H deformation and N–H stretching vibrations, and the broad band at 1300 cmÀ1 demonstrates the C–H and C–N in-plane deformation vibration, respectively UV–Vis...

Ngày tải lên: 20/03/2014, 13:08

... of 10 kOe Results and discussions The structures of the PPy/Zn0.5Cu0.5Fe2O4 nanocomposite were investigated by FTIR spectroscopy and X-ray diffraction FTIR spectra of the PPy and PPy/Zn0.5Cu0.5Fe2O4 ... deformation vibration at 1302 and 1053 cm− 1, C–N stretching vibration at 1190 cm− 1, Fig XRD patterns of PPy/Zn0.5Cu0.5Fe2O4 nanocomposite (a) and the reference standard data for Zn0.5Cu0.5Fe2O4 ... (a) and PPy/Zn0.5Cu0.5Fe2O4 nanocomposite (b) well-stirred reaction mixture The reaction was carried out at °C under nitrogen while stirring for h The nanocomposite was obtained by filtering and...

Ngày tải lên: 20/03/2014, 13:08

... Alonso-Nuñez G Synthesis and Structural Characterization of Mo–Ni–W oxides nanostructures J Nanosci Nanotechnol 2008;8(6):2983–9 [23] Solarska R, Alexander BD, Augustynski J Electrochromic and structural ... of the material for the peaks O1 s and W4f are shown in Fig 6a and b respectively Peaks position for O1 s is 530.3 eV and binding energy peak located at 35.4 and 40.6 eV is attributed to W4 f ... large scale synthesis of WS2 nanotubes from WO3 nanorods prepared by a hydrothermal route Solid State Sci 2005;7:67–72 [19] Ha J-H, Muralidharan P, Kyung Kim Do Hydrothermal synthesis and characterization...

Ngày tải lên: 20/03/2014, 13:08