synthesis production and characterization of liposomes

... low production yields of the enzymes In this article, the cloning, production and characterization of a novel tyrosinase from the filamentous fungus T reesei is reported Results Isolation of a ... Foundation of Raisiogroup (Raisio, Finland), the Finnish Funding Agency for Technology and Innovation (TEKES) and the Commission of the European Communities, specifically RTD program ‘Quality of Life and ... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment...

Ngày tải lên: 19/02/2014, 06:20

... sequence of the expression clone was confirmed by sequence analysis of both DNA strands Production and purification of ADH E coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a single ... gene encodes a protein of 348 amino acids and a calculated molecular mass of 37.823 kDa The sequence belongs to the cluster of orthologous groups of proteins 1063 (TDH and related Zn-dependent ... Machielsen and J van der Oost Metals and inhibitors The effect of several salts, metals and inhibitors on the initial activity of Pf-TDH was checked using butane2,3-diol as substrate in the standard...

Ngày tải lên: 16/03/2014, 14:20

... proteins and characterized from structural and enzymatic points of view The immunoreactivity of these two proteins has also been studied Results Production, isolation, and spectroscopic and structural ... candidate for a major determinant of the immunoreactivity of these proteins Aspf1, another protein belonging to the ribotoxin family, is a major and one of the best-characterized allergens of ... characteristics of the NH2terminal b-hairpin of ribotoxins, we have studied its involvement in functional and immunoreactive properties of the protein On the basis of these ideas, the allergen Aspf1 and...

Ngày tải lên: 16/03/2014, 19:20

... a function of temperature • Characterization of the morphology and particle-size of the synthesized TiO2 powder • Densification studies of the sintered specimens • Characterization of mechanical ... calcinations time and temperature have a great influence on the particle size and phase purity of the final powder 2.6 Sintering of Nanopowder Synthesis, characterization and processing of nanocrystalline ... from the raw materials selection, synthesis and characterization of the as-synthesized and calcined nano-TiO2 powder, to powder consolidation and characterization of the sintered structures 3.1...

Ngày tải lên: 02/07/2014, 12:57

... enzymes and their distribution in the soil layers References Abuzinadah R.A & Read D (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants I Utilization of peptides and ... As shown in Table I, A rubescens and L subdulcis secreted larger amounts of exocellular proteases than C geophilum and H crustuliniforme, although growth of the former fungi was considerably ... R.,A., Finlay R.D & Read D.J (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants 11 Utilization of proteins by mycorrhizal plants of Pinus contorta New F’hytoG 103, 495-506...

Ngày tải lên: 09/08/2014, 04:20

... potential, and addition of a TPP-moiety has been used to drive the selective uptake of a wide variety of Ó FEBS 2003 Fig Selective uptake of mitoDC-81 by mitochondria and subsequent alkylation of mtDNA ... the vicinity of mtDNA with the resultant binding and selective alkylation leading to a depletion of mtDNA in intact cells (Fig 1) Here we report the synthesis and characterization of a novel mitochondria-targeted ... mitochondria and cells In addition to facilitating the development of antigenomic therapies, such a reagent might also be useful in investigating the rate of turnover of mtDNA and in the derivation of...

Ngày tải lên: 20/02/2014, 11:20

... parameters and transport characteristics of Bip-[3H]Pro Results and Discussion Synthesis, chemical characterization and stability of Bip-Pro Figure shows the structure (Fig 1A) and the synthesis ... cells and 10 and 100 lm in SKPT cells) in the presence of increasing concentrations of Bip-Pro (0– 100 lm and 0–50 lm, respectively) The results are Fig Interaction of Bip-Pro with PEPT1 and PEPT2 ... concentrations and at increasing Bip-Pro concentrations The diffusional component of [14C]Gly-Sar uptake, of 8% in Caco-2 cells and of 4% in SKPT cells, measured in the presence of excess of Gly-Sar...

Ngày tải lên: 07/03/2014, 05:20

... Influences of temperature and pH on enzyme activity For the determination of the optimal temperature of the enzyme, the reaction was carried out at 25, 30, 35, 40, 45, 50, 55, 60, 65 and 70 °C and the ... activity of N-D-AAase is affected by the presence of metal ions Thus, the enzyme was treated with EDTA, EGTA, 1,10-phenanthroline and metal ions at concentrations of and 10 mM The presence of the ... Purification and properties of D-aminoacylase of Streptomyces olivaceus Agric Bio Chem 42, 107–113 Ó FEBS 2002 19 Tsai, Y.C., Tseng, C.P., Hsiao, K.M & Chen, L.Y (1988) Production and purification of D-aminoacylase...

Ngày tải lên: 08/03/2014, 16:20

... morphological property of SiNWs, a FE-SEM observation was carried out after synthesis of SiNWs Fig 1(b) shows the topview (15° tilted) and cross-section (see inset of Fig 1(b)) FE-SEM images of the SiNWs ... at λ b 700 nm [20] The difference of the reflectance might be the effect of synthesis conditions, such as thickness of metal film and SiH4 gas flow The reduction of reflectance is very helpful to ... Therefore, further reduction of the reflectance will be expected from the optimization of the synthesis conditions of SiNWs For analysis of the electrical properties, the I–V curve of the fabricated SiNWs...

Ngày tải lên: 16/03/2014, 15:12

... investigation of the termination of the nanowire lateral sides and apex Electron diffraction (ED) and analysis of zero-order and higher-order Laue-zones allows precise determination of unit cell and space ... nanowire, (d) STEM-HAADF image of a nanowire, and (e) linescan of the HAADF signal (solid line) and numerical fit of the shape of the nanowire (dashed line) As both composition and phase can be considered ... operating parameters for production of SnO2 , ZnO, and In2 O3 nanowires Despite the deposition technique is relatively simple, cleanness of the alumina tubes and purity of the atmosphere are the...

Ngày tải lên: 16/03/2014, 15:25

... sidechains of Glu332 of each rat Kv1.2 a-subunit (Kv1.2 channel is composed of four a-subunits) and Arg10, Arg19, Lys30 and Lys33 of Pi4 The Lys26 sidechain of Pi4 enters into the ion channel pore and ... binding of [125I]apamin without any other ligand, and B is the binding in the presence of the indicated concentrations of competitor Abscissa axis is the logarithm of the molar concentration of competitor ... atoms of the P-domain selectivity filter Residue Tyr35 of Pi4 is involved in a hydrophobic cluster of aromatic residues consisting of Trp344, from one of the four Kv1.2 a-subunits, and Trp345 and...

Ngày tải lên: 17/03/2014, 10:20

... large amount of water and hydroxyl groups existed in the tubular products because of the existence of a bending vibration of H O H at 1630 cm−1 , and a strong stretching vibration of O H Fig EDX ... reaction of bulky rutile powders and 10 M NaOH solution without using any templates and catalysts The inner and outer diameters of the nanotubes were approximately and nm, respectively The amount of ... the inner of the nanotubes, forming an interesting composite structure that possesses both the surface properties of rutile nanocrystals and most morphology and mechanical properties of titanate...

Ngày tải lên: 19/03/2014, 16:48

... / Journal of Alloys and Compounds 433 (2007) 216–220 Fig (A) TEM image of the sample produced at 140 ◦ C for h (B) TEM image of the sample produced at 140 ◦ C for h The insets of (A and B) show ... phase of hematite ␣-Fe2 O3 and exhibits that each porous fusiform ␣-Fe2 O3 nanocrystalline was a single crystal Fig 5A and B shows scanning electron microscope images of a typical sample of fusiform ... stainless steel autoclave of 50 mL capacity Finally, the autoclave was filled with distilled water up to 75% of the total volume, sealed and maintained at 140 ◦ C for 4, 8, 12, and 24 h, respectively...

Ngày tải lên: 19/03/2014, 16:48

... control over the shapes of nanowire bundles and nanorods, allowing the formation of urchin-like crystalline h-WO3 via simple substitution of NH4 + in place of Na+ ions of EDTA salt solutions Experimental ... growth of aggregate particles is facilitated and followed by the growth of 1D nanorods to form the urchin-like structure TEM and HR-TEM micrographs of h-WO3 nanostructures formed using Na+ ion- and ... voltammograms of (a) h-WO3 nanowire bundles, and inset Figure, CV curves of urchin-like, were measured in 0.1 M H2 SO4 at a scan rate of 100 mV/s for 10 cycles and (b) CV curves of h-WO3 urchin-like...

Ngày tải lên: 19/03/2014, 16:48

... intensity of the second and ®fth lines of the spectra, in the case of oxides This behaviour can be explained by the preferential orientation of the platelike particles, parallel to the surface of the ... enhancement of the M lines can be a measure of the orientation of the particles at the surface of the green body To illustrate, Fig shows (a) the transmission and (b) CEMS spectrum of the platelike ... ®ltered and then oxidised by drying at 110 C in air F Another path in the hematite synthesis was the direct hydrothermal treatment of an homogeneous mixtures of ferric nitrate (1 M) and urea...

Ngày tải lên: 19/03/2014, 16:48

... temperature of 160 8C and with growth time of h Fig TEM images of TiO2 nanorods grown on PT-substrate at an annealing temperature of 700 8C and at a hydrothermal growth temperature of 160 8C and with ... FE-SEM images of TNAs grown on PT-substrate at an annealing temperature of 700 8C and at a hydrothermal growth temperature of 160 8C and growth time of h (a) PT-substrate; (b) top view of TNAs; (c) ... distribution of TiO2 nanorods grown on PT-substrate at an annealing temperature of 700 8C and at a hydrothermal growth temperature of 160 8C and with growth time of h Fig SEM image of TiO2 nanorod...

Ngày tải lên: 19/03/2014, 16:48

... positions of the main IR bands which are associated with the structure of the Fig FESEM images of wt% PPy/MWCNT composites synthesized with weight ratio of PSS/pyrrole monomer of (c) 0.1, (d) 0.3 and ... conductivities and these results may be due to the decrease in the thickness of PPy with the presence of high content of PSS In order to understand the role of PSS during the formation of PPy/MWCNT ... spectroscopy of wt% PPy/MWCNT composites synthesized with weight ratio of PSS/pyrrole monomer of (b) 0.1, (c) 0.3 and (d) 0.5 For comparison, the Raman spectroscopy of (a) primary PPy/MWCNT composites and...

Ngày tải lên: 20/03/2014, 13:08

... XRD patterns of PPy/Zn0.5Cu0.5Fe2O4 nanocomposite (a) and the reference standard data for Zn0.5Cu0.5Fe2O4 of the JCPDS file No 77-0012 (b) Fig SEM micrographs of Zn0.5Cu0.5Fe2O4 (a) and PPy/Zn0.5Cu0.5Fe2O4 ... of 10 kOe Results and discussions The structures of the PPy/Zn0.5Cu0.5Fe2O4 nanocomposite were investigated by FTIR spectroscopy and X-ray diffraction FTIR spectra of the PPy and PPy/Zn0.5Cu0.5Fe2O4 ... in-plane deformation vibration of NH+ which is formed on the PPy chains by protonation at 1101 cm− [16], C–H out -of- plane ring deformation vibration at 795 cm− and the C–C out -of- plane ring deformation...

Ngày tải lên: 20/03/2014, 13:08

... source and without the presence of additives Experimental 2.1 Synthesis of WO3 Nanostructures WO3 nanostructures were synthesized by using an agedhydrothermal route A saturated aqueous solution of ... the detector; and a computer-controlled XY stage with a spatial resolution of 0.1 µm with two interchangeable gratings (600 and 1800 g mm− 1) and a confocal microscope with 10, 50, and 100× objectives ... step characteristic of mesoporous materials above the relative pressure (P/Po) of 0.4 and specific surface area (SBET) values between 34 and 35 m2 (Table 2) The formation of a mesoporous material...

Ngày tải lên: 20/03/2014, 13:08

... composition of the sample, and the results can be seen in Figure 4(d) It can be clearly identified that the nanorods were composed of Fe and O elements, and the ratio of the number of atoms Fe and O ... of single nanorod with the length of 0.8 μm and width of 0.6 μm under the high multiple When the reaction temperature was increased to 400℃, the samples were a small amount of nanolines 2.3 Characterization ... (c)－(f) and the side SEM image was shown in Figure 1(b) As seen in Figure 1(c) and (e), the shape of nanorods samples were the appearance of rectangular cross-section and with the length range of...

Ngày tải lên: 20/03/2014, 13:11