swi snfs using chromatin immunoprecipitation chip assay

... since distinguishing members of these pairs is beyond the resolution of Chip2 experimental technology ChIP, chromatin immunoprecipitation score for each method, and the ranked lists were evaluated ... computed from the ranked list of p values, using QVALUE software [32] The solid curve shows the falsediscovery rate (FDR) computed using only targeted chromatin immunoprecipitation experiments (35 targets) ... the two methods as 13 14 15 16 17 Buck MJ, Lieb JD: ChIP- chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments Genomics 2004, 83:349-360...

Ngày tải lên: 14/08/2014, 15:20

... while using co -immunoprecipitation (see above) It seems probable that the data obtained using the FRET approach are more reliable as they are determined on intact cells and membranes The co -immunoprecipitation ... Non-specific binding was defined in the presence of lM (+)-butaclamol Assays were performed in triplicate Co -immunoprecipitation experiments For immunoprecipitation experiments, membrane proteins (500 lg) ... and BaculogoldTM DNA (Pharmingen) instead of Bac-N-BlueTM DNA All the viruses were purified using plaque assay purification and amplified by serial infection of Sf9 cells Ó FEBS 2003 3930 L Gazi...

Ngày tải lên: 21/02/2014, 00:20

... damage caused by radiation, 5-FU or oxaliplatin as well as combinations thereof by using a microcolony survival assay and comparing the difference between single and split dose radiotherapy Methods ... of chemotherapy For each different dose level, five mice were treated Microcolony assay A microcolony survival assay [15] was used to analyze crypt survival after treatment Three days after radiotherapy ... and 40% (Fig 2A), which indicates a stronger cytotoxic effect than previous studies using the microcolony assay for 5-FU [20] One explanation for this lower clonogenic cell recov- Regarding oxaliplatin,...

Ngày tải lên: 09/08/2014, 10:20

... 18 Abbreviations AUC: area under the curve; bp: base pairs; ChIP: chromatin immunoprecipitation; ChIP- chip: ChIP- on -chip; ChIP- Seq: ChIP- sequencing; EEMB: early embryonic; modENCODE: model organism ... quantified using RNA-seq Pol II binding across the genome at different stages was profiled using ChIP- seq All the other chromatin features were profiled using ChIP- chip experiments These chromatin ... regression (SVR) models The genome-wide data for chromatin features and gene expression were generated by the modENCODE project using ChIP- chip /ChIP- seq and RNA-seq experiments, respectively (b)...

Ngày tải lên: 09/08/2014, 22:23

... RIN to target fruit-ripening specific genes Methods Chromatin immunoprecipitation ChIP experiments were performed as previously described [6] using ripening tomato fruit at the pink coloring stage ... Enrichment levels of the CArG boxes by ChIP were monitored using quantitative real-time PCR (qChIPPCR) as described below The IPed DNA pool was used for qChIP-PCR as a template The pre-immune ... were assayed by quantitative-PCR analyses (qChIP-PCR) for the regions containing the putative RIN-binding sites described above, and then enrichment levels of the regions were evaluated The qChIP-PCRs...

Ngày tải lên: 11/08/2014, 11:21

... AG and Figure SEP3 expression analysis and chromatin immunoprecipitation (ChIP) AG and SEP3 expression analysis and chromatin immunoprecipitation (ChIP) Confocal Scanning Laser Microscopical ... formaldehyde-crosslinked -chromatin immunoprecipitation Trends Biochem Sci 2000, 25(3):99-104 Buck MJ, Lieb JD: ChIP- chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation ... tiled oligonucleotides (ChIP- chip approach, [9,10]) The identification of target genes from MADS domain proteins by ChIP has been reported recently [5,7,11] A drawback of ChIP is that for each...

Ngày tải lên: 12/08/2014, 05:20

... [9,14-16] Specifically, some of these modified culture assays such as phage amplifica- http://www.tbiomed.com/content/6/1/4 tion assays, line probe assays and colorimetric redox-indicator methods can ... nucleic acid amplification (NAATS), phage amplification and line probe assays for MDR [10] Despite the advent of these modern TB assays, largely because there are no inexpensive technology platforms ... based on prediction of prior infection using the purified protein derivative (PPD)-tuberculin skin test (TST), detecting active TB by sputum smear staining using the Zeihl Neilson Stain (ZN) or...

Ngày tải lên: 13/08/2014, 16:21

... identified the Swi1 , Snf5 and Swi2 ⁄ Snf2 subunits of the SWI ⁄ SNF complex as direct targets bound by several acidic activators [32] Subsequently, two partially redundant regions of Swi1 and Snf5, ... of the Swi1 and Snf5 activator interaction domains, it was necessary to investigate whether the selected genes were direct targets of the SWI ⁄ SNF complex We therefore used a chromatin immunoprecipitation ... bound by SWI ⁄ SNF under our experimental conditions The activator-binding domains of Swi1 and Snf5 are required for promoter recruitment of the SWI ⁄ SNF complex We next studied the level of SWI...

Ngày tải lên: 07/03/2014, 00:20

... Colorimetric assay of endo-b-galactosidase activity As shown in Fig 3, a novel method for endo-b-galactosidase assay using was designed on the basis of the results described above This assay involves ... Compound was assayed with A orientalis b-NAHase as a coupled enzyme and 3–5 with almond b-D-glucosidase using the newly developed colorimetric assay described above Compound was assayed by HPLC ... MS-700 (JEOL Ltd, Akishima, Japan) using 2,5-dihydroxy benzoic acid as the matrix Determination of the amount of protein was carried out using a Bio-Rad protein assay kit Determination of total...

Ngày tải lên: 31/03/2014, 07:20

... Materials and methods Proximity Ligation Assay This study probes 21 putative tumor markers for relevance in pancreatic cancer using a proximity ligation assay (PLA) Multiplex PLA was performed ... Samples were thawed and mixed in a 1:1 ratio with buffer (Olink AB) for undiluted assays or in a 1:50 ratio for diluted assays before incubation for 10 minutes at Page of 12 (page number not for citation ... Logistic regression models were fit using the generalized linear model function in R Survival Analysis and Modeling Survival data were fit to a right-censored model using the Survival function in...

Ngày tải lên: 18/06/2014, 15:20

... before the signals were quantified The SMART assay: capture and detection of the assay signal The RNA signal was assayed by an Enzyme Linked OligoSorbent Assay (ELOSA) The RNA sequence includes regions ... be amplified if required (b) Detection of RNA signal by ELOSA (Enzyme Linked OligoSorbant Assay) The assay uses specific probes: a biotinylated capture probe and enzyme (Alkaline phosphatase, ... to be analysed simultaneously the assay signal and it can be further amplified by the same process if required, and detected by an enzymelinked oligosorbant assay (ELOSA) (Fig 1b) Amplification...

Ngày tải lên: 18/06/2014, 18:20

... much longer time to obtain the same cell count using either a hemocytometer or an automated cell counter Colorimetric assay such as the Alamar Blue assay, although commonly used to assess proliferation ... vascular cells using small interfering RNAs J Am Coll Surg 2007, 204:399-408 doi:10.1186/1479-5876-9-48 Cite this article as: Nabzdyk et al.: High throughput RNAi assay optimization using adherent ... obtain exact cell counts using the Celigo®’s brightfield cell segmentation function (Figure 2A & 2B) In order to address this issue, AoSMCs were trypsinized and manually counted using a hemocytometer...

Ngày tải lên: 18/06/2014, 19:20

... neutralization assay is one of the most trusted and widely used methods employed for the detection of virusspecific neutralizing antibodies [4] The power of the neutralization assay lies in its ... markers, etc.) the neutralization assay remains a proven indicator of serological immunity for many viruses In practice, however, the plaque neutralization assay is a laborious and time-consuming ... analysis was performed on the largest subset of data to determine whether within assay variability would change when using the improved counting method We evaluated all of the tests performed on...

Ngày tải lên: 19/06/2014, 08:20

... before the signals were quantified The SMART assay: capture and detection of the assay signal The RNA signal was assayed by an Enzyme Linked OligoSorbent Assay (ELOSA) The RNA sequence includes regions ... be amplified if required (b) Detection of RNA signal by ELOSA (Enzyme Linked OligoSorbant Assay) The assay uses specific probes: a biotinylated capture probe and enzyme (Alkaline phosphatase, ... to be analysed simultaneously the assay signal and it can be further amplified by the same process if required, and detected by an enzymelinked oligosorbant assay (ELOSA) (Fig 1b) Amplification...

Ngày tải lên: 20/06/2014, 01:20

... purification Conclusion Figure overlays3 Plaque assay for hCoV-NL63 on CaCo-2 cells using different Plaque assay for hCoV-NL63 on CaCo-2 cells using different overlays HCoV-NL63 was serially ... http://www.virologyj.com/content/5/1/138 Figure Plaque assays with different incubation times Plaque assays with different incubation times Plaque assays were performed with Avicel overlay and incubated ... tested for their utility in a cytopathogenic plaque assay Comparison of different overlays Three overlay techniques commonly used for plaque assays were tested for their suitability [23] CaCo-2...

Ngày tải lên: 20/06/2014, 01:20

... much longer time to obtain the same cell count using either a hemocytometer or an automated cell counter Colorimetric assay such as the Alamar Blue assay, although commonly used to assess proliferation ... vascular cells using small interfering RNAs J Am Coll Surg 2007, 204:399-408 doi:10.1186/1479-5876-9-48 Cite this article as: Nabzdyk et al.: High throughput RNAi assay optimization using adherent ... obtain exact cell counts using the Celigo®’s brightfield cell segmentation function (Figure 2A & 2B) In order to address this issue, AoSMCs were trypsinized and manually counted using a hemocytometer...

Ngày tải lên: 20/06/2014, 03:20

... neutralization assay is one of the most trusted and widely used methods employed for the detection of virusspecific neutralizing antibodies [4] The power of the neutralization assay lies in its ... markers, etc.) the neutralization assay remains a proven indicator of serological immunity for many viruses In practice, however, the plaque neutralization assay is a laborious and time-consuming ... analysis was performed on the largest subset of data to determine whether within assay variability would change when using the improved counting method We evaluated all of the tests performed on...

Ngày tải lên: 20/06/2014, 04:20

... the transduction system was modified for application to a chip- based detection method This would premit the fabrication of materials using off-theshelf materials with high stability, which would ... this article as: Hong et al.: Sensitive and molecular size-selective detection of proteins using a chip- based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy ... Principle of heteroliganded gold nanoisland-based sensing platform We examined the feasibility of a chip- based gold nanoisland sensor prepared for the sensitive and molecular size-selective detection...

Ngày tải lên: 21/06/2014, 04:20

... solid-phase dot assay in which the nanoshells are used as color markers for biospecific staining of a drop analyte placed on a nitrocellulose membrane strip Other steps of dot assay technology ... colloidal gold particles [10] The dot assay was carried out on nitrocellulose membranes (0.45 lm pore size; Schleicher & Schuell, Germany) One microliter drops of the assay material (hIgG; Sigma, USA) ... that may be used in the solid-phase dot immunoassay In principle, the silica/gold nanoshells are not the only nanoparticle platform for analogous dot assays and the similar experiments may be still...

Ngày tải lên: 22/06/2014, 22:20

... Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach 53 the binomial distribution in terms ... Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach 55 prevalence, as noted by Brenner and ... combined all these results (932 positive out of 10,289 samples), yielding a prior of B(74.5,748.2) By using the ELISA, of 2,261 serum samples screened 372 were positive, of which 75 samples were confirmed...

Ngày tải lên: 07/08/2014, 17:22