swelling and solubility of granular starch

... folding or of increased solubility of the protein of interest We propose that the success of fusion protein systems should be ascertained only once the protein of interest has been cleaved off and shown ... enhanced solubility phenomenon observed in Pfg27A, we identified three key issues: (a) the role of the N-terminal extension; (b) the role of the D to N mutation; and (c) the role of the sequence and ... presence of 17 extra residues at the C-terminal end It is probable that MBP and the C-terminal extension both contribute to the high yield and solubility of Pfg27A in a complex process of assisted...

Ngày tải lên: 23/03/2014, 21:20

... thermal effects and configurational rearrangements mix, the two can be readily separated (i.e a heap of hot sand will have many of the characteristics of a heap of cold sand) and we can write ... sketch of the density of granular matter in a vessel after being shaken at amplitude A Adapted from [172, 173, 356] Detailed studies of the density of shaken granular systems as a function of the ... functions and forces in granular systems 217 points from the centroid of grain g to the point of its contact with grain g The grains g and g also exert a force on one another through the contact, and...

Ngày tải lên: 01/11/2013, 08:20

... effects of the SG knockout on granular staining properties and storage of proteases, it was first important to determine whether the lack of SG affected the actual assembly of granules and whether ... the amount of actual sulfated PGs is not directly correlated with the level of SG mRNA, and it was therefore of interest to also follow the levels of sulfated PGs during the course of MC differentiation ... after days of culture, and reached a plateau from about day 12 (Fig 2B) Both the kinetics of accumulation and the level of maximal storage were virtually identical in SG+ ⁄ + and SG– ⁄ – cells, indicating...

Ngày tải lên: 07/03/2014, 11:20

... different kind of starch- binding site [34,35] or a second type of SBD consisting of some sequence repeats of unknown structure [36,37] are outside the scope of this work The SBD of the present ... cluster of Bacillus and Thermoanaerobacter CGTases and the remaining enzymes The positions of the two CGTases from K pneumoniae and Nostoc sp PCC-9229 are, however, in agreement with the values of ... Comparison of the domain-level organization of starch hydrolases and related enzymes Biochem J 80, 51–55 Jespersen, H.M., MacGregor, E.A., Henrissat, B., Sierks, M.R & Svensson, B (1993) Starch- and...

Ngày tải lên: 08/03/2014, 08:20

... loss of functional activity The object of this study was to increase the solubility of the C-terminal domain of AMPP, and in so doing to determine which residues are responsible for its poor solubility ... expressed and assayed for solubility, and neither gave rise to detectable levels of protein using the GelCode Blue stain reagent as detector, as shown in Fig 97.4 66.2 Improving solubility of the ... the reduced solubility of the AMPP#3-40 mutant was due to the absence of the R166G mutation, whereas the reduced solubility of the AMPP#3-1 mutant could be attributed to a number of changes (Table...

Ngày tải lên: 23/03/2014, 07:20

... properties of native and annealed starches and their granular residues after 5, 15, and 36 h of hydrolysis by both enzymes as measured by DSC are listed in Table Native and annealed waxy and common ... D Q (1988) Studies on the susceptibility of granular cassava and corn starches to enzymatic attack Part 2: Study of the granular structure of starch Starch – Starke, 40(1), 29–32 ¨ Gallant, D., ... results of enzyme hydrolysis of native and annealed starches are listed in Table 1, and all results are depicted in Fig The results showed that the hydrolysis kinetics of native and annealed starch...

Ngày tải lên: 05/05/2014, 08:43

... details Laboratory of Condensed Matter Spectroscopy and Opto-Electronic Physics, and Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Department of Physics, Shanghai ... the “bottom-up” mechanism of ceramic matrix and thereby may provide a new avenue for nanofabrication With the increasing deposition time of Co, the size or content of granular Co embedded in the ... from that of the superparamagnetism of Co particles in Cu and dendrimer matrix [22,36] Figure shows magnetic hysteresis loops of Co/InP composite structure with the deposition time of for both...

Ngày tải lên: 21/06/2014, 04:20

... absence of light respiration, and Oand Ci are the partial pressures of oxygen and CO inside the leaf, and Kc, Ko are the Michaelis-Menten constants of Rubisco for CO O and Rd, the day 22 and (light) ... 22 °C, 75% of relative humidity, and various levels of CO and under a range of light intensities Spe, 2 cifically, light and CO curves were generated The on total leaf area suming a of the needles ... convexity of the quantum response of the potential electron transport of needles and was fixed at 0.79 (Leverenz and Jarvis, 1979) a is the initial slope of the quantum response curve of potential...

Ngày tải lên: 08/08/2014, 18:21

... affects the charge and separation of enzymes allowing the possibility of increasing the number of zones of enzyme activity It is relevant to note that the R values and f numbers of bands may also ... electrically neutral and not migrate For some pH conditions (B B B and H zones ,, 345 ), of EST-c (Est#6, Est#7, Est#8), zone of ACP (Acp#3), and a few bands of ALP migrated cathodally ... 1990) Some of these factors include the nature of the tissue and type of storage conditions, protocols for enzyme extraction, starch gel preparation and electrophoretic conditions, gel and electrode...

Ngày tải lên: 08/08/2014, 23:22

... three different ears of both Wt and sbe1a genotypes were germinated, demonstrating no Table Kinetics of digestion1 of the resistant starch assay for Wt and sbe1a mutant starch2 Starch y0(%) S1(%) ... of 13 Figure Micrographs of Wt and sbe1a mutant starch samples A Scanning electron micrographs of native starch from Wt (left) and sbe1a mutant (right) Scale bars represent 10 μm at the top of ... produced Analysis of the CL distribution of isoamylase-debranched b-LDs showed a higher proportion of chains of DP ≥ 100 and lower proportions of other chains (DP < 100), before and after pullulanase...

Ngày tải lên: 11/08/2014, 11:20

... JC, Schmele H: Use of ibuprofen and methylprednisolone for the prevention of pain and swelling after removal of impacted third molars J Oral Maxillofac Surg 1995, 53:2-7 Page of (page number not ... tion of pain, inflammation, and fever [3,11] The reduction of biosynthesis of prostaglandins by inhibition of the cyclo-oxygenase enzyme system is considered an important mechanism of action of ... Dionne RA: Comparison of nonsteroidal anti-inflammatory drugs, ibuprofen and flurbiprofen with methylprednisolone and placebo for acute pain, swelling and trismus J Oral Maxillofac Surg 1990, 48:945-952...

Ngày tải lên: 11/08/2014, 23:22

... Standard Test Method of Making, Accelerated Curing and Testing of Concrete Compression Test Specimens Standard Specification for Expansive Hydraulic Cement Standard Test Method of Sampling and ... properties, and including the size and position of all structural components, connections and reinforcing steel, the required concrete strength, and the required strength or grade of reinforcing and ... intrusion of cement paste or water (or both) and the loss of coating materials dur- DESIGN AND CONSTRUCTION OF CONCRETE SILOS AND STACKING TUBES ing concrete placement The anchorage and end fittings...

Ngày tải lên: 24/10/2014, 16:04

... in units of two or three, and the combined action of salivary and pancreatic amylase leads to the production of large amounts of maltose and maltotriose, and relatively small amounts of limit ... of starch hydrolysis (% total starch hydrolysed at different time intervals) of wheat noodles and noodles with addition of different dosage of grape seed extract (GSE) Table 12 Cooking loss of ... concentration of 18 mg/mL and sucrase concentration of 0.15 U/mL; porcine pancreas α-amylase (B) at starch concentration of 10 mg/mL, and amylase concentration is U/mL Figure 16 Eadie-Hofstee plot of acarbose...

Ngày tải lên: 10/09/2015, 09:01

... border to clean up the edges of the selected area of color ߜ Smooth: When you click with the Magic Wand to make a selection of an area of color, you might see all kinds of tiny 1- or 2-pixel areas ... again, and repeat a number of times to create a series of paw prints marching into the distance Figure 8-14: The many faces of transformations The mask-related selection commands At the bottom of ... terms as you read about the various tools and commands with which you make selections Both feathering and anti-aliasing make the edges of your selections softer by using partially transparent or...

Ngày tải lên: 27/08/2012, 14:35

... and all associated documentation are proprietary and copyrighted products Worldwide rights of ownership rest with Computers and Structures, Inc Unlicensed use of the program or reproduction of ... trademark of Microsoft Corporation Adobe and Acrobat are registered trademarks of Adobe Systems Incorporated DISCLAIMER CONSIDERABLE TIME, EFFORT AND EXPENSE HAVE GONE INTO THE DEVELOPMENT AND DOCUMENTATION ... bridge spans 60 feet, and has a width and height of 12 feet each The supports are rollers at one end, and pins at the other The trusses and cross members are to be constructed of 2L4X4’s, while the...

Ngày tải lên: 06/09/2012, 15:56

... and all associated documentation are proprietary and copyrighted products Worldwide rights of ownership rest with Computers and Structures, Inc Unlicensed use of the program or reproduction of ... trademark of Microsoft Corporation DISCLAIMER CONSIDERABLE TIME, EFFORT AND EXPENSE HAVE GONE INTO THE DEVELOPMENT AND DOCUMENTATION OF SAP2000 THE PROGRAM HAS BEEN THOROUGHLY TESTED AND USED ... title of the window reads YZ Plane @ X=24 This same title also occurs on the left-hand side of the status bar at the bottom of the SAP2000 window Click the roof level beam on the left side of the...

Ngày tải lên: 06/09/2012, 15:56

... areas of cost and capacity rather than performance There are two components of disk performance: transfer bandwidth and access time Although both of these factors are improving, the rate of improvement ... us to understand the effects of random access patterns and locality, both of which can be exploited to reduce the cost of cleaning The simulator models a file system as a fixed number of 4-kbyte ... on disk and in terms of how much of the data written to the log they represent More than 99% of the live data on disk consists of file data blocks and indirect blocks However, about 13% of the...

Ngày tải lên: 12/09/2012, 15:05

... generation and recognition are used to communicate between humans and machines Rather than using your hands and eyes, you use your mouth and ears This is very convenient when your hands and eyes ... very specialized audience State -of- the-art researchers need this kind of detailed mathematics to understand the theoretical implications of the work A basic premise of this book is that most practical ... -Simulation and modeling FIGURE 1-1 DSP has revolutionized many areas in science and engineering A few of these diverse applications are shown here Chapter 1- The Breadth and Depth of DSP in many...

Ngày tải lên: 13/09/2012, 09:49

... Social phobia and disruptive social anxiety are features of the lives of many thousands of people But exactly what is social phobia? What causes it? What is its nature and what kinds of treatments ... key concepts and methods and a substantive body of research, this book aims to answer these questions and clarify social phobia by means of critical discussions and examination of evidence It ... clinicians, and students in clinical and health psychology and psychiatry AR I E L ST R AV Y N S K I is Professor of Clinical Psychology in the Department of Psychology at the University of Montreal...

Ngày tải lên: 21/09/2012, 10:54