susceptibility tumour behaviour and their potential as therapeutic targets

... integrins and RTKs is actually considered as critical during normal vascular development and in vascular and inflammatory diseases (Eliceiri, 2001; Streuli & Akhtar, 2009) as well as in tumor ... increased microvascular permeability and angiogenesis EC from caveolin-1 null mice show increased tyrosine phosphorylation of VEGFR-2 and decreased association with VE-cadherin The increased ... reduction in caveolin-1 and cavin-1 (also known as polymerase and transcript release factor) interaction, disappearance of caveolae and increased expression of caveolin-1 at the plasma membrane Importantly,...

Ngày tải lên: 27/06/2014, 17:20

... interleukin (IL)-6 and IL-13, and the serine proteases chymase and tryptase that are capable of activating matrix metalloproteases (MMPs) [9] The implication of serotonin, IL-6, IL-13 and MMPs in PH ... receiving placebo was increased as compared with the healthy rats, whereas there was a decrease of MCs in MCT-rats treated with PLX and CSS (p < 0.05 versus placebo) (Figure 5A) The perivascular MCs ... peribronchial, septal and perivascular areas (Figure 1A) We counted the MCs and found that MC population was about fold higher in IPAH patients as compared to the donors (Figure 1B) There was a preponderance...

Ngày tải lên: 12/08/2014, 13:22

... If there was no response while the neonate was on high ventilatory support and FIO2 of 1.0, INO was increased gradually by ppm to a maximum of 80 ppm If there was a response, INO was maintained ... 20 ppm and FIO2 was gradually decreased to 0.6, provided the PaO2 was 80–120 mmHg Nitric oxide then was weaned to discontinuation Ventilatory parameters thereafter were weaned and HFOV was replaced ... NO gas was introduced into the ventilator circuit via an adaptor positioned on the inspiratory port of the Fisher and Paykel humidification chamber Thus, NO was mixed with the bias flow gas of...

Ngày tải lên: 12/08/2014, 18:20

... experiments performed by Foitzik and colleagues [39] and Wang and colleagues [40], as well as with clinical findings published by Beger and colleagues [24] and Ammori and colleagues [41], who found ... Fritz et al Laboratory Blood was drawn by intracardial puncture for measurement of amylase, lipase, and white and red blood cell count from arterial blood Histological assessment Histomorphological ... complications were evaluated by bacteriological assessments of blood, ascites, pancreas and mesenteric lymph nodes of both the colon and small bowel, as well as by 24-hour mortality rates In a second...

Ngày tải lên: 13/08/2014, 11:23

... RPP and PVA (Table 4) RV ejection fraction decreased (Table 3) as a result of an increase in RV endsystolic volumes and unchanged RV end-diastolic volumes (Figure 4a) RV diastolic function was ... fraction was significantly improved by inhaled iloprost, whereas RV end-diastolic volumes and diastolic function were not affected (Figure 4a and Table 3) LV contractility, diastolic function and afterload ... pulmonary vascular flow-pressure curves and pulmonary vascular impedance Cardiovasc Res 1999, 42:183-192 Ludbrook J: Repeated measurements and multiple comparisons in cardiovascular research Cardiovasc...

Ngày tải lên: 13/08/2014, 11:22

Ngày tải lên: 19/02/2014, 00:20

Ngày tải lên: 09/03/2014, 12:20

... severity as assessed by logit location in Rasch analysis: Items with extreme logit locations (derived from the Rasch analyses) were candidates for exclusion However, it was the aim to include a reasonable ... York Heart Association (NYHA) classes II and III The correlation between the CAMPHOR QoL scores and the CAMPHOR preference based scores was 0.86 Test-retest reliability After removal of cases where ... preference based measure of health status based on general population values should be used to calculate QALYs Generic preference based measures of health status include the EQ-5D [8], the SF6D [9] and...

Ngày tải lên: 18/06/2014, 19:20

... efficacy of ERAs in SScl-associated PAH and in patients in World Health Organisation Functional Class II are awaited The combined vasodilator/antiproliferative/ antifibrotic activity of ERAs raises ... haemodynamics and exercise capacity, compared with baseline values, and that there was no significant difference between the agents [17] Similarly, use of open-label sildenafil as adjunct therapy ... SScl [19] In contrast, the prostanoids improve function and survival in PAH, and may also be antiproliferative In the healthy endothelium, prostacyclin exerts vasodilatory and antithrombotic...

Ngày tải lên: 09/08/2014, 06:23

... [21] was necessary to unmask epitopes for the anti-chymase antibody Measurement of Ang II levels, chymase and ACE activities Tissue Ang II levels were measured by iodine-125 radioimmunoassay ... μl assay buffer, and the radioactivity was measured by a g counter Chymase-like and ACE activities in the lung were also determined by RIA as previously described [22] Briefly, lung tissue was ... up-regulated chymase expression at both mRNA and protein levels in hamster lungs, which was associated with increased artery remodeling, emphysema-like changes and RVSP elevation ACE and chymase-like...

Ngày tải lên: 12/08/2014, 11:21

... holds great potential as a therapeutic strategy for vascular proliferative diseases [33,34] The cell cycle progression is regulated by kinds of cyclin-dependent kinases (CDKs) and their specific ... contribute to PVSR associated with pulmonary hypertension, and up-regulated p27kip1 mediate the inhibition effect on HPH [42] The S phase kinase associated protein (Skp-2) was found as a member of ... 5% CO2 The PASMCs grew out in about a week, and cell passage was performed when the cells grew to 70% confluence Cells were used between passages and Smooth muscle cell identity was verified...

Ngày tải lên: 12/08/2014, 13:22

... hypertension and vascular remodeling in rats: (A) Percent wall thickness *p < 0.05 as compared with normoxia and hypoxic controls # p < 0.05 as compared with normoxia and BMC+CXCR4 $p < 0.05 as compared ... hypertension and right ventricular hypertrophy in rats: (A) mPAP and (B) RV/ (LV+S) *p < 0.05 as compared with other groups; #p < 0.05 as compared with normoxia and BMC+CXCR4; $p < 0.05 as compared ... exposure and femurs and tibias of the rats were dissected sterilely After cutting each end of the femurs and tibias to expose marrow, we placed each bone into a 1.5 ml sterile eppendorf tube and centrifuged...

Ngày tải lên: 12/08/2014, 13:22

... TNF-a and IL-6 (Figure 3) Hydrogen-rich saline treatment decreased MDA and 8OHdG concentrations and increased SOD activity in serum and lung tissues Concentrations of MDA and 8-OHdG in serum and ... higher and SOD activity was lower than in control group It was noted that hydrogen-rich saline treatment for either or weeks significantly decreased the MDA and 8-OHdG levels and increased SOD ... 8-OHdG and MDA levels, and SOD activity in serum and lung tissue Hydrogen-rich saline treatment (5 ml/kg once daily for or weeks) significantly decreased the 8-OHdG (A and B) and MDA (C and D)...

Ngày tải lên: 12/08/2014, 13:22

... interpreted the results and wrote the paper ET and VM co-designed the dispersal and colonization experiments, and coworked on associated data collection and their interpretation SC and PB co-designed ... lung weight was measured by careful excision of the lung from other adjacent extraneous tissues The lung was exposed for 48 h at 180°C and the dry weight was measured Water content was calculated ... demonstrated in vivo in humans [12] as well as in animals [13] suffering from severe infection In particular, increased expression is observed in sepsis and septic shock as well as in LPSexposed animals...

Ngày tải lên: 12/08/2014, 13:22

... pulmonary vascular resistance and increased cardiac output in MCT rats treated with this compound in comparison with placebo group, as evident from both invasive and noninvasive measurements ... model, as evident from TAPSE measurement TBC3711 strongly reduced the RV hypertrophy as evident from invasive measurements of heart ratio (RV/(LV+S)) and RVWT and echocardiographic measurements ... mostly expressed on PASMCs, cardiomyocytes and fibroblasts When expressed on PASMCs, the ET-B receptors together with ET-A receptors mediate vasoconstriction and proliferation of vascular cells, but...

Ngày tải lên: 12/08/2014, 13:22

... EGM and cultured until passage Total RNA isolation and Quantitative measurement Total RNA was extracted from human pulmonary microvascular ECs with an RNeasy Mini Kit (Qiagen, CA, USA) RNA and ... with aSMA to stain transitional cells aSMA staining (blue) was developed with alkaline phosphataseconjugated secondary antibody, and then CD31 staining (brown) was developed with peroxidase-conjugated ... referred to as “proximal” vascular tissue and those obtained from areas after the fibrotic clot region are referred to as the “distal” vascular tissue [11] The tissues were cultured and various...

Ngày tải lên: 12/08/2014, 14:20

... VCAM-1, ET-1 and MCP-1 mRNAs in IL-6-/- and IL-6+/+ during normoxic and hypoxic conditions Lung expression of ICAM-1, VCAM-1, ET-1 and MCP-1 mRNAs in IL-6-/- and IL-6+/+ during normoxic and hypoxic ... in IL-6-/- and IL-6+/+mice This response, as evaluated based on the RVSP increase, was similar in IL-6-/- and IL-6+/+ normoxic mice Therefore, the attenuation of PH development and vascular remodeling ... Exposure to hypoxia was associated with increases in RVSP and Fulton's index in both wild-type and IL6-/- mice However, after weeks hypoxia, RVSP was significantly lower and right ventricular...

Ngày tải lên: 12/08/2014, 14:20

... different from adult disease and potentially more amenable to the therapeutic effect of anti-proliferative medication and thus prevention of early endstage pulmonary vascular disease Competing interests ... coefficient has been calculated to describe the correlation between eNOS and iNOS expression Statistical significance was assumed at p < 0.05 There was a significant correlation of eNOS and iNOS ... short term effect as might be anticipated in cases of PPHN Rather can this increased expression of eNOS persist over months and years as shown in our oldest patients at the age of and years, respectively...

Ngày tải lên: 12/08/2014, 14:20

... progression of the disease process which was characterized by a decrease in interstitial fibrosis and vascular sclerosis, as well as lower expression of collagens I and V and pro-fibrotic cytokines Tolerance ... scleroderma model and nasal tolerance and the immunofluorescence experiments, and drafted the manuscript; WRT contributed to conception and design of the original study and analysis and interpretation ... the fibers of the collagenous system The collagen content was measured in the peribronchovascular and septal interstitium and expressed as a relationship between the quantities of collagen fibers...

Ngày tải lên: 12/08/2014, 14:20