supply demand in experimental pulmonary hypertension

... 2009) Caveolin-1 inhibits eNOS through protein-protein interaction, but it also facilitates the increase in intracellular Ca2+ HSP90 binds to eNOS away from caveolin-1 in Ca2+calmodulin-depedent ... importance of EC including endothelial caveolin-1 in maintaining vascular health 3.3 Inflammation It is well established that inflammation plays a significant role in the pathogenesis of PH Inflammation ... facing the leading edge (Fig 3f) Interestingly, β-catenin was also detected in the nucleus of 32 Pulmonary Hypertension – From Bench Research to Clinical Challenges some separating, detaching...

Ngày tải lên: 27/06/2014, 17:20

... remodeling upon bringing them back to normoxia [22,23] Activated MCs produce several mediators including the biogenic amine serotonin, the cytokines interleukin (IL)-6 and IL-13, and the serine ... Grimminger F, Busch AE, Schafer S: Combined tyrosine and serine/threonine kinase inhibition by sorafenib prevents progression of experimental pulmonary hypertension and myocardial remodeling Circulation ... monocrotaline on the pulmonary vessels resulting in radical tissue injury and inflammatory process [32,33] and increased pulmonary vascular pressure/resistance [34] Taken together, the findings suggest...

Ngày tải lên: 12/08/2014, 13:22

... became INO-dependent and required phosphodiesterase inhibitor (dipyridamole) to wean them from INO Tolazoline was unsuccessfully attempted in two neonates in the referring hospitals Methemoglobin ... the administration of exogenous nitric oxide [15] In addition INO may increase the concentration of phosphodiesterase, which then degrades cyclic GMP when INO is discontinued, resulting in vasoconstriction ... give insight into new therapies that augment the pulmonary vasodilatory effect of INO and the activity of the endogenous NO system In conclusion, the administration of INO at 20 ppm, following...

Ngày tải lên: 12/08/2014, 18:20

... not result in detectable fungal infection In contrast to our experimental standardised setup, patients in the clinical setting often undergo repeated interventional procedures including central ... investigator who was unaware of the experimental design using a scoring system previously described in detail [26] In brief, the head of the pancreas was removed, fixed in 4% buffered formalin ... difficult or near impossible to include enough patients for a meaningful clinical trial • In a standardised setting of experimental necrotising pancreatitis, pancreatic superinfection, bacteraemia and...

Ngày tải lên: 13/08/2014, 11:23

... maintained during the entire procedure using an infrared heating lamp A 7.5-Fr central venous catheter was inserted into the femoral vein A 16-G arterial catheter was advanced into the descending ... the following time points: baseline, and 1, 5, 10 and 30 minutes after stopping iloprost/placebo inhalation In the PHT group, measurements at minute after stopping iloprost/ placebo inhalation ... loops (dotted lines) in one repreanalysis sentative animal at (a) baseline, in (b) pulmonary hypertension, and (c) minutes after inhalation of iloprost These were obtained during a controlled...

Ngày tải lên: 13/08/2014, 11:22

Ngày tải lên: 19/02/2014, 00:20

Ngày tải lên: 09/03/2014, 12:20

... invited to participate in the study Consenting adults were visited in their home for the TTO interview A small pilot study (n = 15) was undertaken in advance of the main study to check that interviewees ... states defined by CAMPHOR I can join in activities with my family and friends Travelling distances is not a problem I feel very dependent I never find speaking too much of an effort I can join in activities ... applied by classifying individuals into particular health states on the basis of their responses to the CAMPHOR For example, an individual who indicates that they can join in activities with...

Ngày tải lên: 18/06/2014, 19:20

... Sitbon O, Robbins IM, Frost A, Tapson VF, Badesch DB, Roux S, Rainisio M, Bodin F, Rubin LJ: Effects of the dual endothelin-receptor antagonist bosentan in patients with pulmonary hypertension: ... inhibitors [5] The use of angiotensin-converting enzyme inhibitors in SScl to prevent scleroderma renal crisis is well established [11] Studies in animal models have suggested that angiotensin-converting ... renin–angiotensin system may prove effective in RP A pilot study of the angiotensin receptor blocker losartan is encouraging In a 12-week randomised trial of losartan versus nifedipine in 25 patients...

Ngày tải lên: 09/08/2014, 06:23

... chymostatin administration significantly reduced the smokeinduced increase in chymase activity but had no effect on ACE activity, suggesting that chymostatin was mainly acting through the inhibition ... smoking-related peripheral lung injury [17] However, it still remains unknown whether chymase is involved in cigarette smoke-induced pulmonary artery remodeling and PAH The role of chymase in ... determined using the bicinchoninic acid (BCA) assay (Pierce, Rockford, IL, USA) The serine protease inhibitor aprotinin (Sigma) and the ACE inhibitor lisinopril (Sigma) were used to inhibit proteases...

Ngày tải lên: 12/08/2014, 11:21

... remodeling [19], etc The molecular mechanisms of estrogen’s cardiovascular protective effects involve decreasing induction of erythropoietin [20], inhibiting endothelin-1 expression [21], initiating ... regulated by kinds of cyclin-dependent kinases (CDKs) and their specific regulatory cyclins The cyclin-dependent kinases inhibitors include INK4 family and cip family which can inhibit the activity ... PASMCs Aiming to know whether p27 kip1 and Skp-2 were involved in chronic hypoxia-induced pulmonary hypertension and artery remodeling, the protein levels of p27 kip1 and Skp-2 in the experimental...

Ngày tải lên: 12/08/2014, 13:22

... therapeutic intervention in pulmonary hypertension Pharmacol Ther 2001, 92:1-20 Rabinovitch M: Pulmonary vascular remodeling in hypoxic pulmonary hypertension In Hypoxic Pulmonary Vasoconstriction ... that inhibition of systemic CXCR4 through administration of AMD3100 will inhibit hypoxia-induced pulmonary hypertension and vascular remodeling in rats and that specific inhibition of the CXCR4 in ... CXCR4 is involved in regulation of bone marrow cell migration during development of pulmonary hypertension and vascular remodeling induced by hypoxia This finding also demonstrated the involvement...

Ngày tải lên: 12/08/2014, 13:22

... saline, fixed in 4% paraformaldehyde for 24 h, and embedded in paraffin Sections of μm were stained with hematoxylin-eosin (H-E) for light microscopy Determination of TNF-a and IL-6 levels in ... hydrogen-rich saline one week after MCT administration was included in our study, in order to avoid having the antioxidant activity of hydrogen-rich saline interfere with the transformation of MCT in the ... of Progressive pulmonary vasculitis leads to increased vascular resistance and a gradual increase in arterial pressure beginning approximately days after a single dose of MCT [18] In our study,...

Ngày tải lên: 12/08/2014, 13:22

... Induction of lung injury by bleomycin Mice received a single intratracheal instillation of saline (0.9% w/v) or saline containing bleomycin sulphate (1 mg/kg body weight) at end-expiration in a volume ... nitrotyrosine is indeed present in lung sections after BLM administration and that AM treatment reduced this staining in the tissues We propose that AM, acting on cytokines, inhibits the iNOS expression, ... studied total TGF-b in lung sections by immunohistochemistry Bleomycin induced a remarkable increase of TGF-b staining in the alveolar epithelium and in the inflammatory infiltrate at 14 (Figure...

Ngày tải lên: 12/08/2014, 13:22

... monocrotaline (MCT)-induced pulmonary vascular remodelling The rat lung sections were stained with elastica staining for medial wall thickness determination of pulmonary vessels in all experimental ... the index of proliferation (IOP) of perivascular cells in monocrotaline (MCT) model of pulmonary hypertension In situ proliferation of perivascular cells in rat lungs was investigating by using ... TBC3711 on pulmonary artery acceleration time (ACT), cardiac output (CO), total pulmonary resistance (TPR) and oxygenation index (paO2/FiO2) in monocrotaline (MCT)-induced pulmonary hypertension...

Ngày tải lên: 12/08/2014, 13:22

... immunohistochemical staining G), Hematoxylin and Eosin (HE) staining; H), Single staining for a-SMA; I), Single staining for CD31; J), Double staining for CD31 and a-SMA; the magnification was 200× ... staining was DAPI G-J), Immunohistochemical staining of endarterectomized tissue The neointimal layer of distal vascular wall tissues was assessed by immunohistochemical staining G), Hematoxylin ... standard hematoxylin and eosin (H & E) staining was performed The CD31 antibody was used to stain endarterectomized tissue, together with aSMA to stain transitional cells aSMA staining (blue) was...

Ngày tải lên: 12/08/2014, 14:20

... on interleukin-6-induced pulmonary hypertension in rats Chest 2001, 119(2):554-561 Steiner MKSO, Kolliputi N, Mark EJ, Hales CA, Waxman AB: Interleukin-6 Overexpression Induces Pulmonary Hypertension ... circulating levels and pulmonary expression of various inflammatory cytokines and chemokines including interleukin-1beta (IL-1β), IL-6, monocyte chemoattractant protein (MCP-1), RANTES, and fractalkine ... seems increased in many types of human and experimental PH, including monocrotaline-induced PH in rats, suggesting that IL-6 may modulate the extent of PH despite the absence of a hypoxic pulmonary...

Ngày tải lên: 12/08/2014, 14:20

... similar (A) Intensity2of staining forage eNOS, (B) iNOS and (C) nNOS in lungs from infants with pulmonary hypertension and from conIntensity of staining for (A) eNOS, (B) iNOS and (C) nNOS in lungs ... This finding is consistent with the hypothesis that increased eNOS is associated with the initiation of pulmonary hypertension (chronic hypoxic model in rats and infants with pulmonary hypertension ... increased pulmonary vascular resistance secondary to congenital heart disease in infants and children This upregulation appears to be linked to pulmonary hypertension in that it occurs in the pulmonary...

Ngày tải lên: 12/08/2014, 14:20

... collagenous fibers in peribronchovascular and septal pulmonary interstitium, Massons trichrome was used to stain the collagen-containing fibers in blue Also, the Picrosirius staining method [19] ... tolerance induction with collagen V In an autoimmunity model such as SSc presenting a severe fibrotic involvement of organs, the decrease in TGF-beta is interesting, since this cytokine is fibrogenic ... 1:100) according to the labeled Streptavidin-Biotin Complex method used previously in others works [21] To quantify stained cells, a point-counting stereologic method [19] was employed using a reticulum...

Ngày tải lên: 12/08/2014, 14:20