substrate specificity and mode of action

... copolymer composed of 81% of 3HB and 19% of 3-hydroxyvalerate (3HV) (Sigma-Aldrich Japan K.K., Japan) was used as the standard of 3HV unit in PHA These standards were also digested and analyzed by ... symbols, Q and r, indicate the flow rate and the recycle ratio of activated sludge And, subscripts, In, I, II, R, and Eff, indicate the influents, reaction tank I, reaction tank II, recycle, and effluents, ... lyophilized After the addition of 2mL of chloroform and acidified methanol (10% sulfuric acid with around 100mg/L of benzoic acid), the content in the tube was mixed well, and was digested at 100°C...

Ngày tải lên: 05/09/2013, 09:38

... rates and bacterial growth in each sample for the analysis of the growth of tetracycline-resistant or tetracycline-degrading bacteria and the mechanism of tetracycline degradation MATERIALS AND ... Tahara M., Shimizu K and Nisimura T (2008) Monitoring of PPCPs in Aquatic Environments in Japan and Evaluation of Removal Efficiency on Assumption of Water Purification Process, J of Japan Society ... Characteristics of Pharmaceuticals in Wastewater Treatment, - 326 - Journal of Water and Environment Technology, Vol 8, No.4, 2010 J of Water and Waste, 50(7), 587-593 Wen X., Jia Y., and Li J., (2009)...

Ngày tải lên: 05/09/2013, 10:15

... Agricultural Stabilization and Conservation Agents and Agricultural Agents of Cullman, Lawrence, and Morgan countics; and the U.S Forest Service Messrs Coy Stephenson and L J Sandlin of the Hartselle Water ... toxaphenc and BHC content of the water (Tables and 4) Prior to the spring of 1962, the treatment consisted of lime-alum coagulation, sedimentation, rapid sand filtration, chlorination, and pH adjustment, ... Estimates of the total weight of inscctitides (technical grades) used and the area treated are given in Table Each year, toxaphenc, DDT, and BHC comprised over 90% of the total In 1959, 1960, and 1961,...

Ngày tải lên: 18/02/2014, 04:20

... (CseHc1 and CseHc2), B dubia (BduHc1 and BduHc2) and P americana (PamHc1 and PamHc2) were compared with the previously identified insect hemocyanins from T domestica (TdoHc1 and TdoHc2) and P marginata ... marginata (PmaHc1 and PmaHc2), P grandis (PgrHc1 and PgrHc2), Sch americana (‘embryonic hemolymph protein’, SamEHP), T domestica (TdoHc1 and TdoHc2) and L saccharina (LsaHc1 and LsaHc2) were used ... a half-saturation pressure (P50) of  torr and shows moderate cooperativity In our studies, O2-binding kinetics could not be measured because of the small size of most specimens However, we assume...

Ngày tải lên: 18/02/2014, 08:20

... 60% of the original activity after h, and 30% after h The D266NALP I showed 40 and 20% of the original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- and ... 80% of the original activity after h The activity of N263D-Sendai decreased to 45% and 10% of the original after and h of incubation at pH 12, respectively N263D/A266Y-Sendai showed only 10% of ... three-dimensional models of the enzymes were constructed to clarify the location of substituted residues and their interactions with surrounding residues The effective mutation sites of Asp266 and Tyr269...

Ngày tải lên: 21/02/2014, 03:20

... evolution of all of the enzymes of the pathway (Fig 4), it is clear that only about half of the enzymes of the tricarboxylic acid cycle, the major pathway of carbon metabolism in mitochondria of oxygen-respiring ... subunits of PDH, OGDH and OADH are related The functional and evolutionary relationships between the subunits of these enzymes are somewhat complicated In a nutshell, the E1a subunits of PDH and OADH ... mitochondria and glyoxysomes, respectively [42,43] They usually have a molecular mass of  90 kDa and are typically homodimers of 45-kDa subunits [44,45] In the presence of Mg2+, glyoxysomal CS of plants...

Ngày tải lên: 22/02/2014, 04:20

... respectively Given the small size of introns and 10, primers C45U/C45L and C1011U/C1011L were designed to amplify together exons and and exons 10 and 11, respectively Sequencing of exon was performed starting ... components (a, b, c, d, and e) Two of these (a and c) appear to be shared with variants A and F, while components e and d seem to be in common with the A variant Therefore, band b represents the ... (a, c, and e) in the AA and MF profile were different, further heterogeneity of the PAGE components may be suspected To understand the high degree of heterogeneity observed with goat as1-Cas and...

Ngày tải lên: 22/02/2014, 04:20

... and FAD contents of supernatants and pellets were measured in aliquots (580 lL) of neutralized perchloric acid extracts by means of HPLC (Gilson HPLC system including a model 306 pump and a model ... proteinligand, proteinprotein and proteinsurface interaction: development of integrated experimental and computational approaches to the study of systems of pharmaceutical interest and from ` ... amount of FAD increased almost linearly in the rst 10 of the reaction, at a rate of 413 pmolặmin)1ặmg)1 protein The amount of FAD reached a maximum value of 3600 pmolặmg)1 protein within 15 of incubation...

Ngày tải lên: 16/03/2014, 04:20

... levels of any resistance to first-line drugs (8.8%) in Ndola, and for the first time systematically investigated and documented absence of resistance to second-line drugs (OFL and KAN) These levels of ... irregular drug intake, inadequate treatment of prior TB episode, prior history of TB, and prior use of rifamycins and rifabutin in treatment of TB and other bacterial infections [22–24] Further, ... McHugh, and S H Gillespie, “Comparison of fitness of two isolates of Mycobacterium tuberculosis, one of which had developed mulit-drug resistance during the course of treatment,” Journal of Infection,...

Ngày tải lên: 22/03/2014, 18:20

... Effect of MAP2 and tubulin on the reactivation of different substrate enzymes (A) Time course of reactivation of 0.05 lM LDH: Self-folding (trace 1, j); in presence of 0.05 lM MAP2 (trace 2, m); and ... NADH and oxaloacetate as substrates [31] All denaturation and renaturation reactions were carried out at 25 °C The extent of refolding was calculated by taking the ratio of the activity of the ... presence of 0.4 lM tubulin (trace 3, d) (B) Time course of reactivation of 0.06 lM a-glucosidase: Self-folding (trace 1, j); in presence of 0.05 lM MAP2 (trace 2, m); and in presence of 0.5 lM of tubulin...

Ngày tải lên: 23/03/2014, 12:20

... occurrence of brown adipocytes in WAT of C57BL/6J mice at 24 °C and after cold exposure We therefore measured (a) the density of multilocular cells in WAT, and (b) the levels of UCP1 mRNA and protein ... whole BAT and WAT The expression of UCP1 mRNA was measured in genital WAT, and in interscapular BAT of C57 WT and b3KO mice (Fig 3) In genital WAT of WT mice (Fig 3A), the basal level of UCP1 mRNA ... d and 10 d, respectively) The number of multilocular (ML) cells is expressed as a percentage of the total amount of adipocytes in the pooled sections of the two WAT depots n ¼ 3–4 *P < 0.05 and...

Ngày tải lên: 23/03/2014, 20:22

... rates of glycolysis from glucose and gluconeogenesis from glycerol The inhibition of glycolysis is consistent with a lowering of the Fru-2,6-P2 concentration and an inhibition of phosphofructokinase-2, ... incubated in the absence of glucose (Table 1), the bolus addition of mM glycerol resulted in an accumulation of intracellular Gro-3-P and depletion of ATP; the concentration of Fru-2,6-P2 fell by ... apparent net synthesis of glucose of 0.62 ‹ 0.05 lmolámin)1á(g wet weight))1 The rate of glycogen synthesis of 0.13 ‹ 0.01 lmolámin)1á(g wet Table E€ect of a bolus addition of glycerol on hepatic...

Ngày tải lên: 24/03/2014, 03:21

... calibrated by addition of standard aliquots of Ca2+ to the medium A standard curve was obtained from the pooled results of five independent series of determinations and used for analysis of mitochondrial ... (2.2 ± 0.1 and 0.8 ± 0.05% of the total mitochondrial activity of GDH and AAT, respectively) (Fig 3B, columns a and c) As shown in Fig 4, the total activities of the matrix enzymes GDH and AAT were ... number of cristae, destructurization of the matrix compartment, a dilated and paled matrix, lack of dense granules (Fig 1C); and (c) altered mitochondria (*) with clear vacuolization of the matrix...

Ngày tải lên: 24/03/2014, 04:21

... pathway of GDP-L-fucose in mouse (Eur J Biochem 271) 79 Fig Gene structures of the short and the long splice variants of mouse fucokinase (A) and human fucokinase (B) The long splice variant of mouse ... L-fucokinase and pyrophosphorylase The primer and probe sequences and their positions in the mRNA sequence, for GDP-L-fucose pyrophosphorylase and the short and long splice variants of L-fucokinase, ... lane 2, short splice variant of mouse fucokinase and lane 3, long splice variant of mouse fucokinase Fig Fucokinase activities of the cell lysates of COS-7 cells (A) and HeLa cells (B) transfected...

Ngày tải lên: 30/03/2014, 13:20

... Saturday that most of the 118-man crew died Aug 12 when a huge explosion Chief of Staff of the Russian Northern Fleet Mikhail Motsak Monday officially confirmed the deaths of 118 crewmen on board ... “Organization”, “Event” and “Things” The topic can be viewed as an object and the series of questions can be viewed as asking for the attributes of the object In this point of view, to find out ... the clustering of attributes of topic category is for the convenience of building a batch QA system When a batch QA system is processing a series of questions under a topic, some of the questions...

Ngày tải lên: 31/03/2014, 01:20

... Interestingly in spite of being a high risk exposure environment the high security forensic psychiatry and the relationship between, and occurrence of job satisfaction, burnout and post traumatic ... the central and northern parts of Norway with about 1.1 million inhabitants The unit consists of wards with a total number of 21 beds One of the wards (ward F) is specialised to serve offenders ... attacks on staff Assessments of professional quality of life were made with the Professional Quality of Life Scale (ProQOL) [16], which is a validated development of the Compassion Fatigue Test...

Ngày tải lên: 18/06/2014, 18:20

... current study, we compared Taq and HF-2 polymerases in terms of sensitivity and robustness, and in terms of evaluating HCV QS diversity and complexity as assessed by CFA of the E2-HVR1 on baseline ... years Immunology and virology ancillary studies including QS analysis are aimed at increasing our understanding of the complex interactions of virus and host in this debilitating and widespread ... + + + + + - - - Figure Comparison of the sensitivity of the E2-HVR1 PCR using Taq and HF-2 enzymes Comparison of the sensitivity of the E2-HVR1 PCR using Taq and HF-2 enzymes RNA was extracted...

Ngày tải lên: 19/06/2014, 08:20

... and 4) The Fairbank classification showed an increase in osteoarthritis of grade in 52% of the patients, 35% of the patients had an increase of grades and 3% of the patients had an increase of ... proximal and distal point of graft (Linvatec, Largo, FL femur 20 of 25/7 mm, tibia of 8/20 of 25 mm) No supplementary fixation was used [28] Full extension of the knee was ensured before insertion of ... the remaining laxity (KT-1000) and the grade of OA (Kellgren and Fairbanks score of the operated knee, difference in Kellgren and Fairbanks score between operated and contralateral non operated...

Ngày tải lên: 20/06/2014, 01:20