subcellular fate of proteins and nucleic acids

... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... nucleic acids; (ii) a small molecule ligand or ligands (for example gluthatione), as a nucleic acid label, and an appropriate receptor or protein fragment which binds the ligand as part of the ... specific sequences) for nucleic acid-binding proteins and should also encode corresponding nucleic acid-binding proteins The use of known protein transcription factors and their target DNA sequences...

Ngày tải lên: 11/08/2014, 00:21

... releases of produced water and petroleum and the improper disposal of produced water are national issues that concern managers of Federal, and State lands, as well as oil and gas producers and lessees, ... siltstone, and thin sandstone beds The geologic and climatic settings of the Lester and Branstetter leases resemble that of much of the major southern mid-continent oil- and gas-producing area of the ... part of the site is underlain by 1) a surface layer of eolian sand (20-70 cm thick); 2) colluvial apron and alluvial deposits of varying thickness comprised of sandstone pebbles, cobbles, and...

Ngày tải lên: 14/02/2014, 03:20

... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser and ... building blocks of eukaryotic chromatin [15] A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been well documented and includes monomethylation and Fig ETD ... breakthrough in the field of proteomics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer The scan times required for direct analysis of proteins using ETD...

Ngày tải lên: 18/02/2014, 16:20

... the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ... amino acids and the necessity to purify each individual sample In contrast, cell-free systems allow the synthesis of [15N]-labelled proteins with very small quantities of [15N]-amino acids and ... combinations of [15N]-labelled amino acids The most abundant amino acids are labelled in only one of the samples, while the least abundant amino acids are labelled in up to three of the samples...

Ngày tải lên: 07/03/2014, 12:20

... cleavage and C18 RP-HPLC has two additional amino acids (Gly and Ser) at the N terminus and a theoretical mass of 10307.6 Daltons (16) A detailed account of the expression and characterization of rCP10 ... (e.g., PicoFuge, Stratagene, Palo Alto, CA) Methods 3.1 In-Gel Digestion (see Notes and 9) 3.1.1 Excision of Protein Bands (spots) from Gels Rinse the entire gel with water and excise bands of interest ... dishes, and so on) may acquire a static charge and attract dust Accumulation of even a minute amount of dust in solutions and reagents results in massive contamination of samples with human and sheep...

Ngày tải lên: 11/04/2014, 09:50

... destination (37% of the proteins; decreased volume in 66% of the proteins of this group was observed) Most of the proteins associated with dormancy breaking in the presence of ABA (Fig 3B) were ... pattern of 71 proteins, with a shift away from accumulation of a major group of proteins, involved mainly in energy and protein metabolism [5] The same negative effect of ABA was observed on proteins ... maturation of enzymes, is necessary for protein assembly and subcellular targeting and controls the activity of enzymes, regulatory proteins and peptides Proteases are thus involved in all aspects of...

Ngày tải lên: 12/08/2014, 03:20

... induction of non-transcriptional responses to nucleic acids A common feature of the recognition of non-self nucleic acids by TLRs, RLRs and DAI is their ability to induce robust activation of genes ... localization of nucleic acid-specific TLRs is important in preventing their contact with self nucleic acids [5] TLR9 was the first TLR identified to interact with nucleic acids, and its classical ligand ... to pDCs and B cells, where the nucleic acids can activate TLR7 and/ or TLR9, resulting in the secretion of type Volume 9, Issue 3, Article 211 Chi and Flavell 211.5 I interferons by pDCs and the...

Ngày tải lên: 14/08/2014, 08:20

... analyses of single protein and simple protein complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) ... proteomics: Analysis of proteins and protein complexes by chemical cross-linking and mass spectrometry Zhuo A Chen Thesis for the Degree of Doctor of Philosophy The University of Edinburgh August ... of Pol II and Pol II-TFIIF complexes 42 44 2.2.1 The Pol II complex and the Pol II-TFIIF complex 44 2.2.2 Cross-linking titration of Pol II and Pol II-TFIIF complexes 45 2.2.3 Cross-linking of...

Ngày tải lên: 30/09/2015, 05:51

... studies of proteins both in vitro and in vivo v List of Tables Table Page The influence of C-terminal residues on the in vivo cleavage of EGFP-intein and on-column cleavage/biotinylation of EGFP ... Biotinylation of three model proteins 24 3.2.1 Cloning of target genes into pTYB1 expression vector 24 3.2.2 Expression and extraction of fusion proteins 25 3.2.3 Affinity purification and on-column ... C-terminus of the yeast gene A standard PCR mixture contained 1x HotStarTaq DNA polymerase buffer (Qiagen), 0.2 mM of each dNTPs (NEB), 0.5 µM of each primer, 100 ng of plasmid DNA template and units of...

Ngày tải lên: 08/11/2015, 16:30

... sparger, and numerous inlets and outlets for removal of samples and medium and for adding various solutions to the growing culture These include nutrients and growth factors to support growth and ... General layout of the bacterial cultivation process for production of proteins and other biological products 10.2.2 Instrumentation and Infrastructure Required To conduct a process of recombinant ... to these elements, cells need phosphorus for the formation of RNA and DNA, and sulfur for the formation of the amino acids methionine and cysteine Other trace elements, such as metal ions, are...

Ngày tải lên: 24/04/2016, 10:44

... sequences of CgueCMV-1.1 and CgueCMV-1.2 (B) Pairwise alignments of the UL56 and gB proteins and the partial DPOL proteins of CgueCMV-1.1 and CgueCMV-1.2 are shown Dots represent identical amino acids, ... (gB), and two thirds of the ORF UL54 (DPOL) (Figure 6B) The nucleotide and amino acid sequence differences of CgueCMV-1.1 and CgueCMV-1.2 were 0.6% and 0.5% (UL56), 20% and 8% (UL55) and 1% and ... sequence C Figure LNA-based and bigenic amplification of beta- and gammaherpesviruses LNA-based and bigenic amplification of beta- and gammaherpesviruses Schematic diagram of the analysis strategy...

Ngày tải lên: 18/06/2014, 18:20

... Detection of nucleic acids Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic acids ... versatility of the electrode was tested Cyclic voltammetry was used for the detection of two nucleic acids samples mentioned above and the basic electrochemical behaviour of nucleic acids at the ... suitable tool for detection of nucleic acids We show the successful application of modern nano-technologies not only for detecting of nucleic acids but also for distinguishing of all bases signals Methods...

Ngày tải lên: 21/06/2014, 03:20

... optimum of stability of monomeric proteins and of their complexes [10] This suggests that pH-dependent proper­ ties of the monomers and of the complexes coevolved at the same pH The presence of a ... more detailed inventory of H+ in subcellular compartments based on the known number and calculated pKa of weak acids and bases, and on emerging data of protein localization and concen­ trations ... many of the physical and Garcia-Moreno: Journal of Biology 2009, 8:98 98.4 biological constraints that guided the evolution and the adap­ ation of proteins to the pH and ionic properties of t...

Ngày tải lên: 06/08/2014, 19:21

... sources of contamination in PCR-based studies of XMRV: (i) MLV-encoding nucleic acids present in commercial PCR reagents, (ii) trace amounts of mouse genomic DNA in human blood and tissue samples, and ... claims of MLV-related viruses in prostate cancer and CFS patients Future assessments of the prevalence of XMRV should include more rigorous PCR and phylogenetic tests to exclude the possibility of ... Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses Retrovirology 2010 7:112 Submit your next manuscript to BioMed Central and...

Ngày tải lên: 13/08/2014, 01:20

... Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization The nucleic acid is a polymer consists of monomers called nucleotides Each nucleotide composes of a nitrogenous ... .142 Table 4.3 Typical concentration and purity of p185-ssDNA standard 144 xii LIST OF FIGURES Figure 1.1 Basic principle of nucleic acids hybridization and detection on a solid surface ... .1 1.1 Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization .1 1.1.2 Label-free detection of nucleic acids 1.1.2.1 Electrochemical ...

Ngày tải lên: 12/09/2015, 09:28

... Liebner S., Andbjer B., Kalbacher H., Wolburg H., Haller H., and Fuxe K Organization of choroid plexus epithelial and endothelial cell tight junctions and regulation of claudin-1, -2 and -5 expression ... cytoplasm and nucleus and that the subcellular localization of claudin-1 may dictate the metastatic capacity of melanoma cells Our findings suggest that nuclear versus cytoplasmic expression of claudin-1 ... amino acids long), but all of these are also sites of PKA phosphorylation (Table 1) There are however, three unique sites of PKA phosphorylation, at amino acid residues 65-69, and 189-192 and 201-205...

Ngày tải lên: 03/11/2012, 11:17

... roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and treatment of these conditions Suppression of ... Induction Fig Schematic diagram showing the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases The expression of ANGPTL2 and ANGPTL6 ⁄ AGF is induced in obese conditions ... inflammation and systemic insulin resistance through the induction of vascular inflammation and monocyte migration ANGPTL6 ⁄ AGF antagonizes obesity and insulin resistance through the enhancement of systemic...

Ngày tải lên: 14/02/2014, 22:20

... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were analyzed as arithmetic means of separate gel runs The number of gel runs ... SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-terminal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs 6H4, SAF60, SAF70 and...

Ngày tải lên: 19/02/2014, 02:20

... PGM1 and PGM4 targets) Effects of DNA cis-platination on sequencespecific DNA binding of p73 proteins We tested the influence of DNA modification with cisplatin on the binding of two p73 isoforms, ... with preparation of the p53 proteins, and Professor G Melino for donation of plasmids pcDNA3-HA-p73b and pcDNA3-HA-p73d This work was supported by IGA MH CR grant No NC ⁄ 7574-3 and partly by GACR ... respective supershifted bands are denoted as SR53, SR73d and SR73b; the presence of two supershifted bands in each of the lanes 5–7 corresponds to two possible stoichiometries of the antibody–protein...

Ngày tải lên: 19/02/2014, 05:20