studies of variable vector cell contact and adsorption periods
Báo cáo sinh học: " The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events" pot
... Transduction – studies of variable vector- cell contact and adsorption periods 293T cells were grown in 24-well plates to approximately 70% confluence The cells were incubated with 500 µl of pHR' CMVLacZ ... depth of relevance to diffusion), vector decay and the period of vector adsorption (and thus vector decay) We also examined the use of various MOIs on several commonly used cell lines and tried ... the number of transduced cells (Figure 2a) At hours less than half of the active vectors had adsorbed on to the cells Since vector adsorption to cells was often protracted, the issue of thermostability...
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Báo cáo y học: "Genetic polymorphism of p53, but not GSTP1, is association with susceptibility to esophageal cancer risk – A Meta-Analysis"
... located in epithelial cell of the mouth throat or lungs and adenocarcinoma is composed of cells of glandular tissue Over the past decades, many changes in the prevalence of esophageal cancer have ... studies with 2919 cases and 4074 controls for p53 Arg72Pro and 1885 cases and 2194 controls for GSTP1 Ile105Val 301 Materials and Methods Identification and Eligibility of Relevant Studies We attempted ... Arg72Pro) and Val allele (for GSTP1 Ile105Val) frequency (MAF) were 0.44 and 0.20 for Asian studies, while around 0.60 and 0.32 for Mix and Caucasus populations, respectively Table Characteristics of...
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Báo cáo y học: "TPO, but not soluble-IL-6 receptor, levels increase after anagrelide treatment of thrombocythemia in chronic myeloproliferative disorders"
... model of action of TPO with its receptor on target cells It is well established that serum levels of TPO are regulated by the binding of the ligand to the receptor and the endocytosis of the ligand-receptor ... reduction of the platelet count with at least 50 % of the baseline value, and treatment failure (F) as a reduction of the platelet count with < 50 % of the baseline value Here, and for the purpose of ... magnitude of the change of platelet counts, on the one hand, and changes of TPO or sIL-6R concentrations, on the other Neither were there any significant correlations between changes of TPO or...
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Five stories that are almost true, but not quite
... very special and prosperous section of expatriate Hellenism was returning to the motherland after a century and a half of residence in Egypt to become diluted and anonymous A sense of superiority ... her, she is thousands of miles away in distance and already three days past in time They seem more like three months A deadly combination of space and time I got out of bed and left my room I ... of Egypt They had produced poets, novelists and artists of international renown, scores of philanthropists that endowed their fortunes to build schools, hospitals and stadiums both in Egypt and...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... induced the appearance of an 68-kDa band in wild-type, Y18F, Y29F, Y197F and Y310F tau, this band was not apparent on western blots of lysates of Y394F tau (Fig 2A) The absence of this larger species ... bands of 64 kDa in the GST–FynSH3-bound fraction from cells transfected with each of the tau constructs Densitometric analysis of Fyn-SH3bound tau as a proportion of that in corresponding cell ... NaCl ⁄ Tris and harvested in lysis buffer, as described above Preparation of GST fusion proteins and GST pulldown Isolation of DRMs Generation of GST, GST–Fyn-SH2 and GST–Fyn-SH3 and subsequent...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... Measurement of cytosolic cAMP and InsP3 Intracellular levels of cAMP and InsP3 in resting and activated platelets were measured as described previously [27] Basal concentrations were 2.59 ± 0.17 and ... thrombin-induced Ca2+ response, and abolished the effects of ADP, AR-C and wortmannin A B C D Fig Contribution of SERCA and phospholipase C to P2Y12-dependent prolongation of Ca2+ responses (A, B) Fura-2-loaded ... importance of the PI3-Kb isoform PI3-Kb and not PI3-Kc mediates P2Y12-dependent procoagulant activity of mouse and human platelets Prolonged elevation in [Ca2+]i can signal for surface exposure of phosphatidylserine,...
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Tài liệu Báo cáo khoa học: Activated Rac1, but not the tumorigenic variant Rac1b, is ubiquitinated on Lys 147 through a JNK-regulated process docx
... Ubiquitination and proteasomal degradation of Rac1L61 and Rac1b (A) Activated Rac1L61 undergoes ubiquitination in HEK293 cells Ubiquitination of Rac was assessed by transfecting HEK293 cells with ... form of Rac1, in the presence of UbR48 (C) Ubiquitinated forms of Rac1L61 accumulate in the presence of the proteasomal inhibitor MG132 HEK293 cells were transfected with a combination of expression ... panel) HEK293 cells were transfected with a combination of expression plasmids of HA–Rac1L61, HA– Rac1bWT, HA–Rac1bL61 and 6His–myc–Ub as indicated Proteasomal degradation of Rac1L61 and Rac1b (right...
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Cytoskeleton reorganization mediates alpha beta integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells pdf
... numbers, and RGD on subpyknotic anti-α6 different ratios follicles culturedpeptides Effect of6 rates ofIgG on cellsmall of LNproliferation and Effect of anti-α6 IgG on cell numbers, proliferation and ... cytochalasin D on cell numbers, proliferation and pyknotic rates of GC cultured on LN substratum Effect of3 Effect of cytochalasin D on cell numbers, proliferation and pyknotic rates of GC cultured ... (percentage of labeled cells) and the pyknotic index (percentage of pyknotic cells) after 48 h of culture This has been shown to allow the be the optimal culture time for the study of the effects of various...
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Blackwell Science, Ltd Group breeding dramatically increases reproductive success of yearling but not older female scrubwrens: a model for cooperatively breeding birds? ppt
... risk of starvation of young or vigilance might reduce the risk of predation, and so increase the reproductive success of the group In extremely poor conditions, however, helpers may be of little ... bred both in the Gardens and in the adjacent Canberra Nature Park (c km2 ), and there was dispersal both into and out of the Gardens The Gardens occupy an area of 40 ha, of which 27 are planted ... Journal of Animal Ecology, 70, 370– 385 Social groups are territorial throughout the year, and usually consist of a single breeding pair, or a trio of a female and two males (about 10% of groups...
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Dangerous But Not Omnipotent pptx
... coordination of meetings and flow of information We would also like to thank Andrew Hoehn, Vice President and Director, RAND Project AIR FORCE, and David Ochmanek, Director of PAF’s Strategy and Doctrine ... who conceived of the study and offered valuable guidance, advice, and encouragement David Ochmanek also offered a useful and thorough critique of draft versions of this monograph RAND colleagues ... should be of interest to U.S security policymakers, military planners, and analysts and observers of regional affairs in the Middle East and Central and South Asia RAND Project AIR FORCE RAND Project...
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Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx
... outline of the process leading to the solution of crystal structures and after discussion of the appearance of the electron-density maps and the indicators of quality of both the experimental data and ... number of ligands, the geometry of the coordination sphere, and the B-factor of the ion and its environment For example, the distance between ˚ calcium and oxygen atoms should be 2.40 A and ˚ ... X-rays – the shape and symmetry of the cell define the directions of the diffracted beams, and the locations of all atoms in the cell define their intensities The larger the unit cell, the more diffracted...
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Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot
... consumption of glucose and the synthesis of ethanol in yeast cells treated with H2O2, which produced a decrease in both the consumption of glucose and synthesis of ethanol The apparent effect of H2O2 ... study of oxidative response of S cerevisiae, and of other cell types, to stress can be focused under different aspects: the oxidative defence systems of the cell, inducible adaptive responses and ... rates of disappearance of substrates and appearance of products (Fig 5B) Inhibition of glycolysis as a plausible theoretical explanation for increased inosine Yeast cells treated with H2O2 and...
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Báo cáo Y học: Ruk is ubiquitinated but not degraded by the proteasome ppt
... (B) An aliquot of each cell lysate was immunoblotted with anti-EE antibody to check expression of Ruk isoforms (C) Fig Heterodimerization of Ruk L with Ruk M and Ruk S Hek293 cells were transfected ... and 200 lM for chloroquine (Chloro)] Cells were lysed and the quantity of protein measured by Bradford assay 10 lg of total protein from each cell lysate was separated by SDS/PAGE The level of ... induced by stimulation of cells with EGF and found to be dependent on tyrosine phosphorylation of the C-terminal region of Cbl ([12] and our data, not shown) The same mode of interaction has been...
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Báo cáo khoa học: Amyloid structure – one but not the same: the many levels of fibrillar polymorphism potx
... properties of amyloid assemblies lies in the regularity and repetitiveness of their underlying molecular architecture of intermolecular b-strands stacked perpendicular to the fibril axis [18], often ... atomic resolution structures of the molecular packing of peptides in amyloid-like structures are beginning to emerge [19], much of our understanding of the structure of amyloid-like fibrils comes ... parallel bundles of two or more protofilaments (Fig 1) Fibril preparations often contain several different morphologies, and for some of these the turn lengths and 4592 Fig Comparison of representative...
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Báo cáo khoa học: Insulin is a kinetic but not a thermodynamic inhibitor of amylin aggregation pot
... min, and 200 lL of supernatant of the sample was loaded into an HPLC system for SEC analysis The concentration of amylin was 10 lM, and the concentration of insulin was 100 lM Intensity of ThT ... formation in different time periods The concentration of amylin was 10 lM in each sample The concentration of insulin was 10 lM in (B) and (D), and 100 lM in (E) The identity of each sample is shown ... interaction between amylin and insulin may contribute to the pathogenesis of T2D [2,3,7] Several studies have investigated the effects of insulin on amylin aggregation, and suggested that insulin...
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Báo cáo khoa học: Novel isoenzyme of 2-oxoglutarate dehydrogenase is identified in brain, but not in heart potx
... protein bands of our preparation as follows: E1o (band 1), E2p (band 2), E3 (band 3), E2o and the E3-binding component of the pyruvate dehydrogenase complex (protein X; band 4), E1pa (band 6), ... Processing of the spectra was performed by the use of data analysis software package and biotools software from Bruker A peptide mass tolerance of ± Da, a fragment mass tolerance of ± Da and one ... MS analysis of band In the brain preparation, this band contained both OGDH and OGDHL, a hypothetical isoenzyme of OGDH predicted from the nucleic acid data [16] Our analysis of 10 band samples...
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Báo cáo khoa học: The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH docx
... Effects of intracellular pH on the mitotic apparatus and mitoitc stage in the sand dollar egg Cell Mot Cytoskel 37, 263–270 46 Van Duijn, B & Inouye, K (1991) Regulation of movement speed by intracellular ... interaction of G4–6 with G-actin Discussion The pH-dependence of the G2/G4–6 interface Activation of gelsolin by calcium and/ or low pH has been found to be necessary for the binding of actin [21] and ... helix of gelsolin’s G6 domain binds G2 and that this contact is released upon the binding of calcium We have further shown that residues 203–225 and 159–193 within G2 form the G6 binding site, and...
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Social capital and Health status: a protective impact among elderly or inactive but not among active ? doc
... information sharing, the coordination of activity and the process of collective decision Through the introduction of social norms and sanctions, social network and institutions introduce a social ... health and mortality: a review of twenty-seven community studies" , Journal of Health and Social Behaviour, vol 38, no March, pp 21-37 Islam M.K (2007), “Essays on Social Capital, Health and Socio-economic ... creation and the used of social capital depend not only on the social environment but also on the institutional context Therefore, mutual trust is both the result of institutional context and of social...
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Báo cáo Y học: The C-terminal region of ammodytoxins is important but not sufficient for neurotoxicity pot
... (AtnI2 and AtxA are composed of 121 and 122 amino Fig Alignment, SDS/PAGE and CD spectra of chimeric sPLA2s (A) Amino acid sequence alignment of chimeric sPLA2s with nontoxic ammodytin I2 (AtnI2) and ... AtnI2(N24F)/AtxA(K108N) and 33.3 lM for wild-type AtnI2 Protein water solutions and water were scanned three times in a cell of mm pathlength, the spectra were then averaged and smoothed Binding studies AtxC ... Madison, WI), and the cells were grown at 37 °C in · 450 mL of LB-enriched Enzymatic activity was determined by a slightly modified standard method using a micellar substrate [26] Hydrolysis of egg-yolk...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... calnexin and the heavy chain of HLA-I; ii) that the disruption of the first transmembrane domain of HPV16 E5 modifies the subcellular distribution of the protein; and iii) that the disruption of the ... sequences of E5 and the mutants M1, M2 and M3 Transmembrane Hidden Markov Model posterior probabilities for the sequences of E5 and the mutants M1, M2 and M3 A) Amino acid sequence of the wild-type ... domain of HPV16 E5 prevents the interaction, colocalisation and immunoprecipitation of the viral protein with calnexin, and also of that with the heavy chain of HLA-I Discussion Eukaryotic cells...
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