studies forward and reverse genetics in dissecting functional roles of nachrs

... arginine levels The levels of ornithine, citrulline and arginine remained low, at between 0.01 and 0.04 lmolặg)1 fresh weight (Fig 3E) However, arginine accumulated up to 70-fold and 80-fold in ... 5.8-fold increase in arginine relative to the wild-type plants in air, whereas in high CO2, arginine remained at a wild-type level, indicating that the high level of photorespiratory ammonium was in ... contents in leaves of Arabidopsis plants cultured with mM nitrate in air (F) Ornithine, citrulline and arginine contents in leaves of Arabidopsis plants cultured with mM ammonium in air (G) Ornithine,...

Ngày tải lên: 30/03/2014, 01:20

... coordinators of this programme most especially Prof Marcel Tanner of Swiss TPH and Prof Markus Wenk of National University of Singapore (NUS) and also to our two ladies; Ms Christine Mensch and ... inhibitor binding pocket for in vitro polymerase activity and as well for replication fitness in context of the DENV TSV01 infectious virus For these studies, individual amino acids lining this ... has a C-terminus that interacts with both finger and palm domains resulting in the closed conformation of RdRp during de novo initiation This is to restrict access to the template binding channel...

Ngày tải lên: 02/10/2015, 17:14

... Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of both TnC and Ca2+ Ac, actin; Tm, tropomyosin; ... TnCbinding site and the C-domain of TnC accompanying Ca2+ binding to site IV of TnC In vertebrate troponin, the activation may be a result of the interaction between the inhibitory region and ... 116– 131) In the relaxed state, the inhibitory region binds to actin and inhibits actin–myosin interaction [11,12], while in the contractile state, Ca2+-binding to site I and ⁄ or II of TnC causes...

Ngày tải lên: 20/02/2014, 01:20

... RT domainswapping by introducing three restriction enzyme sites, HpaI (flanking RT amino acids 288/289), SpeI (flanking RT amino acids 423/424) and ClaI (flanking IN amino acids 4/5) creating p8MJ4-HSC ... contains a natural and unique ApaI site in p6 region of gag In addition, the SpeI site in gag and two ClaI sites (upstream of gag initiation codon and in gag) were eliminated to ensure that the introduced ... causing resistance to RTIs have been shown to occur mainly in the DNA pol domain of RT, recent studies have implicated mutations in the Cterminal region of RT in resistance and possibly in restoring...

Ngày tải lên: 13/08/2014, 01:20

... Profile of editing in CaV1.3 IQ motif in different mouse brain regions Figure 2.7 Developmental profile of editing in CaV1.3 IQ motif in mouse and rat brains Figure 2.8 Membrane expression of ... enhancement in membrane trafficking of α1 subunit, associated with an increase in the number of ligand binding sites, current amplitude, faster activation and inactivation kinetics and a hyperpolarizing ... µg) and rat β2a (1.25 µg) and α2δ (1.25 µg), using 0.5 µl of PLUS Reagent and µl of Lipofectamine LTX (Invitrogen, Carlsbad, CA) Cells 32 were incubated in DNA-Lipofectamine LTX mixture for h in...

Ngày tải lên: 10/09/2015, 15:48

... Remanufacturing and refurbishing activities also may be included in the definition of reverse logistics Products may be in the reverse direction in the supply chain for a variety of reasons which include: ... the field of reverse logistics as being relevant for business and society and gave the definition of reverse logistics” Kopicki et al (1993) investigated the discipline and practice of reverse ... existing forward flows Accordingly, the 3PLs are ideally suited in handling reverse logistics and cost savings At present, many companies often handle the reverse distribution through the original...

Ngày tải lên: 08/11/2015, 16:21

... absence of bone remodelling, and satisfactory filling of the infra-bone defects with the graft material in situ After months, radiographs showed presence of mild bone remodelling and excellent infra-bone ... showed good results in manipulating and accelerating the wound healing process in a large number of medical disciplines, as evident in ophthalmology, dermatology, dentistry and rheumatology [10,11] ... known as integrins and belong to the family of cellular adhesion molecules (CAM) [17] Several studies suggest that integrin-mediated interaction plays a decisive role in 67 the regulation of proliferation,...

Ngày tải lên: 03/11/2012, 11:35

... culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ginseng ... cut into sections of 2-10 mm and then were inoculated into MS solid medium (Murashige and Skoog, 1962) containing 30 g/L sucrose, mg/L 2,4-D, and 0.1 mg/L kinetin After month callus were induced ... in the medium The maximum production of ginseng saponin and polysaccharide obtained (1.5 g/L and 2.19 g/L) at the initial nitrogen concentration of 40 mM [12] In the simultaneous production of...

Ngày tải lên: 12/02/2014, 10:20

... effects of dissimilarities in earnings, loan and deposit strategies on performance Yet, differences in the capitalisation and investment in technology and financial innovation of merging institutions ... Herring and G.P Szego (1995), The role of capital in financial institutions, Journal of Banking and Finance 19, pp 393-430 Berger, A N (1995), The relationship between capital and earnings in ... excluding interest, staff and other overheads payments) as a proportion of total assets are included to account for investment in technology and innovation (TECH) Dissimilarities in investments in...

Ngày tải lên: 06/03/2014, 09:22

... residues proposed to be involved in RNA binding and complex formation, and of the C-terminal region for the func- Lsm1 and -8 domains involved in localization tional competence of the Lsm1–7p complex ... the C-terminal domain deleted is consistent with this (Figs and 5) Hybrid proteins carrying the N-terminus of one protein and the Sm domain of the other localize according to the N-terminal contribution: ... grown in SD lacking uracil and methionine Wild-type LSM1 and LSM8 and deletion mutants were amplified by PCR and inserted into multiple cloning sites of pGFP– N-FUS or pGFP–C-FUS Point mutations in...

Ngày tải lên: 07/03/2014, 02:20

... use of their front and back of ce systems for disbursing of loans and collecting loan repayments Another promising track for savings banks is to invest their savings deposits in microfinance institutions ... microfinance institutions Linking savings to microcredits: Offering savings services requires a high level of institutional development to meet safety and soundness requirements Indeed, a large and widespread ... system, instead of relying on informal circuits In designing their products, savings banks take into account the special needs and limitations of lowincome households as well as those of more...

Ngày tải lên: 15/03/2014, 10:20

... was engineered in each plasmid at the junction between the N-terminal and C-terminal domain sequences of each protein The chimeric protein composed of the N-terminal domain of hoPL and of the ... protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility of the active site in the N2 proteins was independent of the nature of the C-terminal domain, in contrast ... composed of the N-terminal domain of guinea pig PLRP2 and of the C-terminal domain of human PL (gpN2 ⁄ huCc) was inactivated at the interface Moreover, it was reported that the C-terminal domain of...

Ngày tải lên: 16/03/2014, 06:20

... be vaccinated every months in combination with training of owners and workers on biosecurity measures and risks of introducing new livestock 2) In villages, the focus will be on vaccinating young ... application of the vaccine Farm managers in Kenya maintain vaccination times a year as it proved to be effective in preventing clinical FMD even in the presence of infected buffalo and sick wildlife in ... elements including, but not limited to, vaccine performance characteristics in relation to circulating virus strains, vaccine coverage, age of vaccinates, maintenance of the cold chain during vaccine...

Ngày tải lên: 18/03/2014, 00:20

... beliefs and behaviors, like other social practices that women and men engage in, are a means for demonstrating various forms of femininity and masculinity In examining constructions of masculinity and ... adopting health-promoting behaviors by engaging Key Determinants of the Health and Well-Being of Men and Boys TABLE 1.3 Thirty-One Key Determinants of the Health and Well-Being of U.S Men and ... gender and men’s health This fi rst chapter examines the main in uences on the health of men and boys in the United States It does so by identifying and discussing 31 key determinants of physical and...

Ngày tải lên: 22/03/2014, 16:21

... for 10-30 min, and then rinsed repeatedly with sterile distilled water The sterilized roots were cut into sections of 2-10 mm and then were inoculated into MS solid medium [7] containing 30 g/L ... The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric acid (IBA), 0.1 mg/L of kinetin and 30 g/L sucrose The pH was adjusted to 5.8 before autoclaving Cells ... Concentration of macro element NH4NO3 KNO3 Determination and analyses Extraction and determination of ginsenoside production were determined as reported previously [8,9] MgSO4 Determination of cell...

Ngày tải lên: 28/03/2014, 10:20

... customary international law is unwritten, it is often reflected in the terms of treaties, and in the writing of legal scholars and experts The principle of command responsibility is incorporated into ... including officers But according to agencies carrying out training on human rights, commanders sometimes stay away from training programs and only send foot soldiers In other instances, commanders ... France, and other countries Since this initial training, several army training programs have been established and implemented, including by the UNHCR, MONUC, the ICRC, and international and Congolese...

Ngày tải lên: 29/03/2014, 09:20

... his politics The missing link: Marcuse and U.S culture The most glaring omission in Reitz’s presentation is an analysis of the links connecting Europe of Marcuse’s youth and the United States ... liberatory power of art against the prosaic routine of daily life He argues that revolutions in art and culture—manifestations of the rebellious spirit of the aesthetic imagination—can fuel social-protest ... men and things, remains the task of political action.” Marcuse’s major contention in this essay is, how- NATURE, SOCIETY, AND THOUGHT ever, that no negation of the alienating conditions of social...

Ngày tải lên: 30/03/2014, 16:20

... appeared during these interviews Almost all the lay counsellors we interviewed were interested in future training and continuing in what was considered a professional field, including obtaining advanced ... contribution of Rebecca Dirks and Dr Justin Mandala of Family Health International, Arlington, Virginia, United States of America, in reviewing the manuscript They also thank clients, staff and the Ministry ... studies to ensure that trainees understand the concepts and methods of HIV counselling and testing In the four-week practicum, each trainee practices counselling and testing http://www.human-resources-health.com/content/7/1/44...

Ngày tải lên: 18/06/2014, 17:20

... nigral dopaminergic cell death induced by 6hydroxydopamine through inhibition of both mixed-lineage kinase 3/cJun NH2-terminal kinase (JNK3) and apoptosis-inducing kinase 1/JNK3 signaling pathways ... in PD patients, implying an intrinsic abnormality of CD200-CD200R signalling in PD brain Interestingly, CD200R expressed in human beings and rats functions only as an inhibitory signal [60] However ... study in PD patients indicate that both intrinsic abnormal CD200CD200R signalling and environmental neurotoxins participate in the pathogenesis of PD According to previous studies, the bolus administration...

Ngày tải lên: 19/06/2014, 22:20

... preparation and conduct of the study and the editing of the article BR contributed to shaping of the article and the editing of the article GEE contributed to the statistical analyses and the editing of ... prediction of risk, information of treatment strategies and preventive measures is well established in the genetic counseling method This finding might therefore be of particularly clinical interest since ... distress when interpreting signs and symptoms for the individuals at risk [11,12] In addition, information of being at risk of a possible life-threatening cardiac disorder and experiencing sudden...

Ngày tải lên: 20/06/2014, 15:20