structures and structure function relationship

... HIV-1 Tat responsive element (TAR) RNA binding, NMR structures of TAR–ligand complexes and aminoglycoside–RNA interactions, we have designed and synthesized a set of conjugates of aminoglycoside ... aminoglycosides Thus, a new set of poly arginine 6-mer and 9-mer d- and l-aminoglycoside conjugates (APACs) was designed and synthesized, and their cell uptake and antiviral activities were determined We ... interaction with CXCR4 and compete with its natural ligand SDF-1a to CXCR4 Results Synthesis and chemical characterization of APACs The synthesis of the regioselectively functionalized aminoglycosides...

Ngày tải lên: 16/03/2014, 06:20

... al Structure reactivity function study of GIF A1 B1 A2 B2 Fig (A1) and (A2) Simulated structure of the b-domain of rlMT2 (B1) and (B2) Predicted structure of the b-domain of hGIF Panels A1 and ... mainly been made using NMR and molecular dynamics simulation Structure of the a-domain of GIF The structures of the a-domain of rat GIF and hGIF were solved by Armitage [10] and our group [9], from ... spectroscopic and biochemical studies Structure reactivity function study of GIF showed that the whole structure and dynamic properties of sGIF, as well as the solvent accessibility and stability...

Ngày tải lên: 16/02/2014, 15:20

... crystal structure) inducing significant alterations of the protein conformation This is also valid for the solution structures as determined using SAXS Superposition of solution and crystal structures ... Superposition of structure models of the ADP-EcODC complex in crystal and solution The crystal structure of 2q28 is shown in ribbon and line style in deepsalmon, the solution structure model of ... program DAMMIN [29] is shown as aquamarin spheres The structures on the left hand side are rotated 90° around the y axis (middle) and z axis (right hand side) C D (Fig 2B) In the case of ThDP enzymes,...

Ngày tải lên: 06/03/2014, 11:20

... shown how evolution of the structure function relationship of a protein can be studied using comparative biochemistry and how hypotheses regarding the structure function relationship can be proved ... chimeric and truncated proteins As structure determines function, and because much current research is associated with human diseases such as amyloidoses, insight into the structure function relationships ... eightstranded b-sheets, one inner (strands DAGHH¢G¢A¢D¢) and one outer (strands CBEFF¢E¢B¢C) (Fig 2) The interactions predominantly involved are hydrogen bonding between two F strands (F, F¢) and...

Ngày tải lên: 07/03/2014, 00:20

... (triplets of 5-H and 6-H at 7.04 and 7.12 p.p.m., doublets of 4-H and 7-H at 7.39 and 7.42 p.p.m and the singlet of 2-H at 7.16 p.p.m with identical integrals of all signals) and to the b-CH2 ... and spin systems of the indole moiety (triplets of 5-H and 6-H at 7.12 and 7.18 p.p.m., doublets of 4-H and 7-H at 7.43 and 7.75 p.p.m and the singlet of 2-H at 7.81 p.p.m with identical integrals ... kDa), and ovalbumin (45 kDa) (Combithek, calibration proteins for chromatography, Ó FEBS 2003 Structure function studies of E cloacae IDPC (Eur J Biochem 270) 2325 Boehringer Mannheim GmbH) and...

Ngày tải lên: 08/03/2014, 02:20

... and SMART [30] databases, and through further PSI-BLAST and fold recognition experiments Modelling of the common conserved region of clostripain was carried out with MODELLER [31] using the structures ... Secondary structure in experimental structures was dened with STRIDE [41] Results and discussion Kinetics of reaction of BDD with clostripain Incubation of ChC with 5.65148.6 lM BDD at pH 7.6 and 25 ... predicted for clostripain) The caspase and gingipain structures shown (1bmq [32] and 1cvr [35], respectively) both contain inhibitors bound at the catalytic site and covalently attached to the catalytic...

Ngày tải lên: 23/03/2014, 12:20

... ‘acid’ loop between the b1 and b2 strands and an 11-residue ‘basic’ loop between the a3 helix and the b12 strand (Fig 2B) Both loops (largely disordered in the crystal structures) are located in ... or directly (steric contacts) Further structural and functional evidence will be required to fully understand and validate such models Structure and role of the intracellular region With few exceptions ... Four structures of full-length (D1 + D2) intracellular regions have been reported to date: LAR [67], CD45 [68], RPTPr (NYSGC, PDB ID: 2fh7) and RPTPc (Oxford SGC, PDB ID: 2nlk) The LAR and CD45 structures...

Ngày tải lên: 30/03/2014, 04:20

... Tat variants and almost no α-helix [10] However, the secondary structures of Tat are dependent upon its environment and change dramatically with an αhelix becoming the main secondary structure in ... Chaloin O, Le Grand R, Loret E, Briand JP, Roques B, Desgranges C, Muller S: Specificity and effect on apoptosis of Tat antibodies from vaccinated and SHIV-1-infected rhesus macaques and HIV-1-infected ... Country France France USA Gabon India South Africa Brazil DR Congo DR Congo DR Congo Uganda Uganda Uganda Thailand Clade B B B B C C C D D D D D A CRF_AE Figure Tat sequences representative of the...

Ngày tải lên: 12/08/2014, 23:21

... papa and mama for your upbringing and everything, my sisters and my brother-in-law, for taking care of me and my parents since I left Malaysia more than 11 years ago Thanks, my wife, your love and ... Wu and Matijevic-Aleksic, 2005; Gross and Weitz, 2008) 1.3.3 Direct thrombin inhibitors Limitations of heparin and warfarin drive the continual and intense efforts to develop new, efficacious and ... thrombostasin • • • Single domain of ~ 16 kDa Eight-stranded (A-B-C-D-E-FG-H) β-barrel and a central ligand-binding pocket Directional inversion in B and C strands compared to typical lipocalin topology...

Ngày tải lên: 14/09/2015, 14:11

... function, whereas D2 connects D1 to the membrane The crystal structure of rCD2 D1 revealed that nine β strands sandwiched in two β sheets of AGFCC’C’’ and BED with four well-defined hairpin structures ... C, C’ and C’’ β-strands and the FG, CC’, and C’C’’ loops (Figure 1-7 (B)) [48, 49] Crystal structure of hCD2-hCD58 complex showed that the center of the contact area is the C, C’ stands, and the ... array and is linked to CD2-mediated adhesion and activation events [31] Figure 1-2 Topology of domain of CD2 and CD58 (a) Crystal structure of rCD2 D1 [30] (b) human CD2 D1[27, 33] and (c) NMR structure...

Ngày tải lên: 04/10/2015, 15:46

... mutations are noticed at the level of function Bio-molecule function, in turn, is directly related to 3D structure As such, by studying and comparing the sequences and 3D structures of the different human ... identification and functional analysis of human SDR family on sequences and structures is the primary goal of the study leading to new targets for drug design and development 1.3 Aims and Objectives ... important for the function and for stabilization the secondary and three-dimensional structures of the protein That is, it can illustrate which residues and regions that represent conserved functional...

Ngày tải lên: 23/10/2015, 15:38

... (* all possible structures for trans and cis products were shown in Figure 11) 23
 Figure 26 Acid-catalyzed cyclization of imine (* all possible structures for trans and cis products ... mixture (* all possible structures for trans and cis products were shown in Figure 11) 25
 Figure 28 Acid-catalyzed cyclization of imine (* all possible structures for trans and cis products were ... proton j 15
 Figure 16 Structures of isomers E and Z 15
 Figure 17 NMR spectra of imines (b) and (a & c) in DMSO-d6 16
 Figure 18 Equilibration of Z and E isomers to a common...

Ngày tải lên: 26/11/2015, 10:48

... Analytical enzymes and enzyme substrates were specied in previous papers [1518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc StP and apo-Ec MalP ... citrate and 0.1 M cysteine hydrochloride, in short, the resolution buffer Various pH values between 5.0 and 8.0 were tested, and a pH of 7.0 was chosen (see below) Prior to the resolution, CcStP and ... R226A and R242A mutants (Table 1), all enzymes were stable for h in the presence of mM potassium phosphate and potassium sulphate Reconstitutions with PLP of apo-Cc StP and mutants thereof, and...

Ngày tải lên: 19/02/2014, 16:20

... hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands These ether strands mimic the distance between the nucleobase and the diphospho-bridge, but on the other hand ... investigation in the future Structure activity relationship of cADPR In-depth reviews covering the chemistry and biological activity of many cADPR analogues have been published [16,53–55] and the reader interested ... permeabilized T-cells and induced both local and global Ca2+ signalling in intact T-cells [46] 8-Azido- and 8-NH2-cIDPRE performed similarly (Fig 4) whereas the halogenated compounds 8-Br- and 8-Cl-cIDPRE...

Ngày tải lên: 20/02/2014, 01:20

... FLUID -STRUCTURE INTERACTIONS SLENDER STRUCTURES AND AXIAL FLOW VOLUME FLUID -STRUCTURE INTERACTIONS SLENDER STRUCTURES AND AXIAL FLOW VOLUME MICHAEL P PAIDOUSSIS ... elements, monitoring and control tubes, thin-shell structures used as heat shields in aircraft engines and thermal shields in nuclear reactors, jet pumps, certain types of valves and other components ... ultimately found some direct or indirect, and often very important, practical application *See also Becker (1981) and Paidoussis (1986a 1991) SLENDER STRUCTURES AND AXIAL FLOW (ii) to serve as a vehicle...

Ngày tải lên: 13/02/2014, 16:20

... [28] The amino acid sequence identity is 34% between dNK and dGK [29], and 47% between dGK and dCK [28], and the structures of dNK, dGK and dCK appeared to be very similar [27,28] Despite the ... the structure and function of the enzyme by mutating Val106 to amino acids differing in polarity, size and conformation, and subsequently investigating their effect on the quaternary structure and ... glycerol and mM dithiothreitol) with and without 2.5 mM ATP, and incubated on ice for h before storage at )80 °C The enzymes incubated and stored with and without ATP are referred to as the +ATP and...

Ngày tải lên: 16/03/2014, 16:20

... analyze and understand the structure function relationships This review deals with the structure function relationship of each individual domain as well as the holotoxin 4468 S Swaminathan Crystal structures ... and the structures were expected to be similar Indeed, individual domains are similar although E differs from A and B in the domain organization The crystal structures of individual domains and ... substrate peptides and specific scissile bonds are unique to BoNTs The crystal structures of holotoxins BoNT ⁄ A, B and E have been determined and have given insight into the function and mechanism...

Ngày tải lên: 22/03/2014, 15:21

... Molecular modeling The structures of monomeric and dimeric variants of BS-RNase were calculated from the NMR and X-ray structures deposited at the PDB In particular, the NMR structure of mBS (PDB ... DHv:H: from CD and DSC curves, relative to the unfolding of the secondary and tertiary structure respectively, are for each dimer very close, indicating simultaneous collapse of both structures As ... Ribonucleases: Structures and Functions Academic Press, NewYork Merlino A, Ercole C, Picone D, Pizzo E, Mazzarella L & Sica F (2008) The buried diversity of bovine seminal ribonuclease: shape and cytotoxicity...

Ngày tải lên: 22/03/2014, 17:20

Ngày tải lên: 23/03/2014, 03:20