... close.PREPARED BY SANDRA DANIELAn effective five paragraphessay places the thesis, or argument, at the beginning of the essay so the writer can offer support for the argument in subsequent paragraphs. ... three supporting paragraphs that follow.PREPARED BY SANDRA DANIELHow To Write An Effective Five Paragraph Essay PREPARED BY SANDRA DANIELTopic: The death penalty should be abolished. Do ... make an argument but organizational skills as well. A well-structured essay should draw the reader in, reveal a strong argument, offer effective support, and come to a logical close.PREPARED...
... Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similarto what is observed in VPRK. Both PRK and VPRKhave calcium bound at Ca3. SPRK also has an aspar-tic acid ... 1022–1025.39 Perrakis A, Harkiolaki M, Wilson KS & Lamzin VS(2001) ARP ⁄ wARP and molecular replacement. ActaCrystallogr Sect D Biol Crystallogr 57, 144 5– 145 0. 40 Jones TA, Zou JY, Cowan SW & Kjeldgaard ... subtilases is capable of accommodating at least sixamino acid residues (P4–P2¢; notation according toSchechter and Berger [ 14] ) ofa polypeptide substrateor inhibitor [15], and both main chain and...
... Payan F, Theys F, Qian M, Haser R &Gerday C (19 94) Stability and structural analysis of alpha-amylase from the antarctic psychrophileAlteromonas haloplanctis A2 3. Eur J Biochem 222, 44 1– 44 7.51 ... polarity of protein surfaces or differentdegrees of packing [42 ,44 ,47 ].Cold-adapted 1SH7 and mesophilic 1IC6 have a lar-ger solvent accessible surface area and a larger non-polar surface area ... proteinase encompasses amino acids 140 42 0 of the 530 amino acid prepro-enzyme [ 34] . The modelwas refined at a resolution of 1. 84 A ˚with an R-factor of 14. 1% and an Rfreevalue of 19.6% (Table 1).Figure...
... experimentally determinednumerical values of the ratio a/ b, and r a is the theoreticalnumerical value of ratio a/ b for a mixture of aromaticamino acids (Tyr and Trp), containing the same molarratio ... photosystem II complex of spinach. Biochim. Biophys. Acta 936, 46 5 47 4.22. Kuwabara, T., Murata, T., Miyao, M. & Murata, N. (1986)Partial degradation of the 18-kDa protein of the photosyntheticoxygen-evolving ... not mark any as pooror inappropriate. Another structural analysis, obtained bytheVERIFY3Dprogram [61], gave an average value of 0.21,which is greater than zero, the quality value indicated...
... theutilization of oxygen by Desulfovibrio gigas. Eur J Bio-chem 216, 44 3 44 8.32 Sakuraba H, Takamatsu Y, Satomura T, KawakamiR & Ohshima T (2001) Purification, characterization,and application ofa ... Significantly, this structural feature of NOXtp is highly similar to that of valosine-containingprotein-like ATPase from Th. acidophilum, an archaealmember of the AAA family (ATPases associated ... with a variety of cellular activities) [ 24] . In addition, the structure of the cysteine mutants, NOXtpC4 5A, NOX-tpC12 2A and NOXtpC4 5A ⁄ C12 2A, was the same asthat of the wild-type. Thus, it appears...
... (1997) Quantification of information transfer viacellular signal transduction pathways. [Erratum (1997)FEBS Lett. 41 9, 150] FEBS Lett 41 4, 43 0 43 4.20 Posas F & Saito H (1998) Activation of the ... cerevisiaeMAP kinase cascades typically composed of three tiers of protein kinases, a MAP kinase (MAPK), a MAPK kinase(MAPKK) and a MAPKK kinase (MAPKKK), arecommon signalling modules in eukaryotic ... differential equations. Pac Symp Bio-comput 4, 29 40 .8 Arkin A, Ross J & McAdams HH (1998) Stochastickinetic analysis of developmental pathway bifurcation inphage lambda-infected Escherichia...
... HinCel 6A and CcCel6C (Fig. 2)indicated that Asp150 and Asp3 34 of CcCel6C arethe potential catalytic residues and could act as a proton donor and a base, respectively. Another aspar-tic acid ... Igarashi3,Masahiro Samejima3, Kiyoharu Fukuda1, Atsushi Nishikawa2and Takashi Tonozuka21 Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Japan2 ... Similar knobs, which areformed by Arg 140 and Gln433, are observed at theentrance of the cleft of HinCel 6A, but the distancebetween atom NH1 of Arg 140 and atom NE2 of Gln433 is only 6.9 A ˚(Fig....
... organicrinorganichybrid materials due to their applications in catalysis,sorption, energy storage, molecular electronics, optical ma-wxterials and ceramics 1 4 . A promising synthetic routetakes advantage ofa ... synthesized and structurally characterized. The2102 310 structure of title compound consists of infinite chains running parallel to the b axis, that are made up of distorted WO octahedra linked6through ... low-temperature soft approach, hy-drothermal method, and the structure- directing function of organoamines. Recent developments in this area haveproven its viability for the successful preparation of...
... Trypanosomatidae and theglycosomal redox balance of insect stages of Trypanoso-ma brucei and Leishmania spp. Mol Biochem Parasitol 149 , 155–169.16 van Hellemond JJ, Simons B, Millenaar FF &Tielens ... compilation ª 2009 FEBS 2831 of the alanine of the AXXCH motif (Ala25) (ingreen) and the unsaturated vinyl group of the heme(cyan) are separated by 3 .41 A ˚(as compared with a typical thioether ... R value: a novel statisticalquantity for assessing the accuracy of crystal structures.Nature 355, 47 2 47 5. 49 Cruickshank DW (1999) Remarks about protein structure precision. Acta Crystallogr...
... at both ends. In the case of Xgh7 4A, the active cleft is open. Although a precise anal-ysis of the mode of action of Xgh7 4A has not beenperformed, Xgh7 4A appears to be an endoglucanasebecause ... Collaborative Computational Project N (19 94) TheCCP4 suite: programs for protein crystallography. ActaCrystallogr D 50, 760–763.18 Navaza J (19 94) AMoRe: an automated package formolecular replacement. ... Gusakov AV, Markov AV, Ustinov BB,Semenova MV & Sinitsyn AP (20 04) Specific xyloglu-canases as a new class of polysaccharide-degradingenzymes. Biochim Biophys Acta 16 74, 268–281. 4 Hasper...
... homologs.Crystal structureof b-lactamase TAE4 fromPse. fluorescens and structure comparison withrelated enzymesCrystals of the b-lactamase TAE4 belong to spacegroup P21, with unit cell parameters a ... enzymes:molecular basis of cold adaptation. Cell Mol Life Sci53, 830– 841 .29 Matsumura N, Minami S & Mitsuhashi S (1998)Sequences of homologous beta-lactamases from clinicalisolates of Serratia marcescens ... France) on a MarResearchCCD.Data processing, molecular replacement andrefinement of Pse. fluorescens TAE4 b-lactamaseData were processed with the hkl suite package [45 ]. A molecular replacement...
... 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’D36N + D 341 N D36N-15’-CATCCTTCCCTATCTCAACATCCAGCTGGTTTACT-3’D 341 N-15’-AAGGGGAGAACTCAACGGAACACCGGAGG-3’D389N 5’-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-3’5’-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3’M. ... [17].Table 3. Primer sequences.TmIDH mutant Primer sequenceR186M 5’-CCTGGAGAAATCGATCATGAGCTTCGCTCAGTCGTG-3’5’-CACGACTGAGCGAAGCTCATGATCGATTTCTCCAGG-3’F205M 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’D36N ... 5’-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-3’5’-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3’M. Karlstro¨m et al. Structure and thermal stability of T. maritimaFEBS Journal 273 (2006) 2851–2868 ª 2006 Karolinska...
... Ragona L, Molinari H, Tava A & Zetta L(2003) Anticarcinogenic Bowman-Birk Inhibitor Isolatedfrom Snail Medic Seeds (Medicago scutellata): Solution Structure and Analysis of Self Association ... respectively, bymeans of standard Lineweaver–Burk analysis of initial ratekinetics. Fitting of the experimental data required as inputdata the initial concentrations of BApNA, enzyme andinhibitor, ... A ˚(rmsd)bb(37–51) 0. 64 A ˚Ramachandran plot statisticsAmino acids in most favoured region 64. 1%Amino acids in allowed region 31.6%Amino acids in generously allowed region 4. 3%Amino acids...