structure mechanism of action and heterologous expression
Mechanism of action and binding mode revealed by the crystal structures of key enzymes in plants sugar metalbolic pathway
Ngày tải lên :
11/09/2015, 09:08
... filtration profile of FRK Crystal of SeMet FRK 3.4 Sample diffraction pattern of SeMet FRK crystal 81 3.5 Crystal structure of HoFRK 85 3.6 Structure based sequence alignment of HoFRK 90 3.7 Stereo ... 2.3.7 SPS-S6P complex 2.3.8 Putative ADP / UDP binding pocket 2.3.9 Mechanism of action Chapter 3: Mechanism of Action and Structure of Fructokinase from Halothermothrix orenii 24 24 24 25 25 26 26 ... 2: Mechanism of Action and Binding Mode Revealed by the Structure of Sucrose Phosphate Synthase from Halothermothrix orenii 2.1 Introduction 2.2 Material and Methods 2.2.1 Cloning, expression and...
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Báo cáo y học: "Ultra-low microcurrent in the management of diabetes mellitus, hypertension and chronic wounds: Report of twelve cases and discussion of mechanism of action"
Ngày tải lên :
26/10/2012, 09:39
... mellitus High levels of free radicals and the decline of antioxidant defense mechanisms lead to damage of cellular organelles and enzymes, increased lipid peroxidation, and development of insulin resistance ... effects on diabetes, hypertension and wound healing Presumably, one of mechanisms of action is its antioxidant activity The action of EPRT is to produce electrical pressure rather than an electrical ... continued to improve and heal, and her remaining gangrenous toes eventually fell off Her blood pressure at admission was 166/53 with use of Lisinopril, which was dropped and eventually ceased...
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Tài liệu Báo cáo khoa học: Antifungal effects and mechanism of action of viscotoxin A3 docx
Ngày tải lên :
19/02/2014, 07:20
... generation of H2O2 in spores [9,15–18] In this work we have gained more information on the cellular targets and the mechanism of action of vis- cotoxins, by examining the interaction of VtA3 with ... monochromator, and Glan-Thompson polarizers using quartz cuvettes with continuous stirring of the suspension, bandwidths of nm for excitation and nm for emission, and excitation and emission wavelengths of ... 79 Analysis of the fungicidal effect of viscotoxin A3 M Giudici et al excitation and emission wavelengths were 360 ⁄ 425 and 362 ⁄ 450 nm for observation of the fluorescence of DPH and TMA-DPH,...
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Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
Ngày tải lên :
24/03/2014, 04:21
... in heterologous bacterial hosts [29] However, coexpression of rPFL with the foldase of P aeruginosa did Fig Alignment of the PFL sequence with Pseudomonas lipases of known three-dimensional structure ... of charged residues on the surface which enhances flexibility and ability of interaction with the solvent [3] Both factors are of importance for cold activity On the other hand, the presence of ... the number of disulphide bridges, the location of proline and arginine residues, and hydrophobic core interactions are generally recognized as key factors in determining the flexibility of the enzyme...
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Báo cáo y học: "Anti-inflammatory effects and possible mechanism of action of lupeol acetate isolated from Himatanthus drasticus (Mart.) Plumel." ppt
Ngày tải lên :
11/08/2014, 03:20
... neutrophils, and is a biomarker for inflammation Furthermore, to clarify the LA mechanism of action, we studied the participation of pentoxifylline, a PDE5 and TNF-alpha inhibitor, and morphine, a mu and ... pre-treatment of mice with LA (12.5 and 25 mg/kg, i.p) significantly reduced the volume (μL) of the edema in the 2nd (31 and 41%), rd (30 and 50%) and 4th (23 and 27%) hours after administration of dextran, ... 4Faculty of Medicine of Juazeiro Norte, Ceará, Brazil Authors’ contributions DL and EL: carried out most of the in vivo experiments MB, HV and AS: isolation and determination of the chemical structure...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
Ngày tải lên :
12/08/2014, 23:21
... study furthers our understanding of the mechanism of action for anti-HIV drug interactions and the efficacy observed for the TDF+FTC+EFV triple combination for the treatment of HIV infection Methods ... Where and represent the individual action of drug or drug 2, while 1,2 represents the combined action of the drug combination The CI values of < 1, = 1, and > indicate synergy, additive effect, and ... and FTC-TP [30]; and (2) DEC or DEC-like complex formation by TFV-terminated DNA and HIV-1 RT in the presence of the FTC-TP or EFV, and by FTC-MP-terminated DNA and HIV-1 RT in the presence of...
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Discovery and mechanism of action study of anti viral compounds for dengue virus
Ngày tải lên :
10/09/2015, 15:51
... need to determine its mode of mechanism During the studies to determine the mode of mechanism of NITD770, host lipid rafts (as suggested by chemogenomic profiling data) and cholesterol were confirmed ... 68 3.2.4 Anti-viral activity of compound NITD448 73 3.3 DISCUSSION 75 A STUDY OF THE MODE OF ACTION OF NITD770, A SMALL MOLECULE INHIBITOR OF DENGUE VIRUS 79 4.1 INTRODUCTION ... STUDY USING MACSYNERGY II 42 2.6 EXPRESSION AND PURIFICATION OF DENV NS5 PROTEIN AND MUTANTS 43 2.7 HPLC/APCI/MS ANALYSIS OF CHOLESTEROL AND ZYMOSTEROL 44 2.8 INDIRECT IMMUNO-FLUORESCENCE...
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Characterisation of the effects and mechanism of action of rapamycin and genistein on acute myeloid leukemia using high throughput techniques
Ngày tải lên :
11/09/2015, 09:18
... understanding the mechanism of action of drugs (Li, et al., 2007; Frolov, et al., 2003) (Nunoda, et al., 2007) Transcriptomic techniques can survey the expression levels of thousands of genes and ... comparison of transcriptome profiles of MV411 and THP-1 75 Figure 4.3 Hierarchical clustering of transcriptomic profiles of microarray data 78 Figure 4.4 Linear regression analysis of fold-changes of ... transcriptomic and proteomic modulations caused by the drug Such a study would go a long way in illustrating the mechanism of action of rapa in AML in its totality and in expanding our cognizance of the...
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The mechanism of action of sprouty2 characterization of the interaction between sprouty2 and PKCdelta
Ngày tải lên :
14/09/2015, 14:06
... representation of the Ras-ERK pathway induced by RTKs 1.3.1.3 The primary structure of Ras isoforms 12 1.3.1.4 Schematic representation of the primary structure of Raf 15 1.6.2 The structure and isoforms of ... the point of receptor-ligand binding, upstream of Ras, upstream of Raf, and at the level of or downstream of Raf (Guy et al., 2003; Kim and Bar-sagi, 2004) The study conducted by Casci and colleagues ... Schematic representation of PLCγ signaling 39 1.7.1 Schematic representation of the primary structure of PLCγ1 41 1.8.1 Primary structure of PKCδ 44 1.9.1 Primary structure of PKD1 53 2.1 Multiple...
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CHART OF MODAL AND SIMILAR EXPRESSION(WITH EXCISES)
Ngày tải lên :
14/09/2013, 01:10
... could/b.would./ c used to/d both b and c) 28 ……… take this letter to the post office, please?(May you/ Must you/ Would you/ none of these) 29 in our family my father ………sit at the top of the table (used to/ ... Noone …………at home(must have been/ can have been/ should have been/ none of these) 17 she’s fainted Throw some water on her face and she ……… Come round.(has to/ may/ must/ can have) 18 ……… you please ... advantage of this opportunity to thank for your co-operation (would like/can/ am able to/ might) 15 you ………… add it up I’m not good at figures.(a.have to/b ought to/c ‘d better/ both b and c) 16...
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Tài liệu BIOCHEMICAL TARGETS OF PLANT BIOACTIVE COMPOUNDS A pharmacological reference guide to sites of action and biological effects doc
Ngày tải lên :
17/02/2014, 05:20
... complex structure and function features of the various groups of phenolics are sketched below The structures of a variety of simple and more complex polycyclic phenolics are presented in the order of ... tens of thousands of individual primary scientific papers and reviews describing the isolation, structural characterization, pharmacological effects and biochemical targets of thousands of plant-derived ... K+-ATPase and Naf, K f -ATPase H Voltage-gated Na+ channel Ligand- and voltage-gated K+ channels + Voltage- and ligand-gated Ca2+channels and ~ a /Ca2+ antiporter CFTR, voltage-gated C channels and...
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Tài liệu Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans doc
Ngày tải lên :
19/02/2014, 16:20
... by the action of cycloheximide Cycloheximide binds to the 80S-subunit of eukaryotic ribosomes and prevents the initiation and elongation reaction of protein biosynthesis The mycelium of pregrown ... cell-free extract and water to a final volume of 980 lL The reaction was started by the addition of 20 lL of a 100 mM ATP solution (final concentration mM) and the reduction of NAD was monitored ... propionyl- Ó FEBS 2004 CoA and acetyl-CoA In the presence of both thioesters one might expect an accumulation of succinyl-CoA in the cell and a deadlock of further reactions of the citric acid cycle...
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Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reflection on some controversial features Elena Ghibaudi and Enzo Laurenti docx
Ngày tải lên :
20/02/2014, 02:21
... survey of the absorption features of MPO, LPO and their intermediates The absorption spectrum of MPO is characterized by an intense Soret peak at kmax ¼ 430 nm and weaker bands at 570, 620 and 690 ... cycles of both LPO and MPO have unusual features with respect to the classical peroxidase mechanism and interactions with H2O2 In the light of the experimental data presented above, we offer the ... a reaction loop involving Cpd III, Cpd II and a ferrous form of LPO Although, they cannot exclude the intervention of H2O2 as electron-donor Such a reaction scheme is cited 4406 E Ghibaudi and...
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Tài liệu Báo cáo Y học: Tissue factor pathway inhibitor A possible mechanism of action doc
Ngày tải lên :
22/02/2014, 04:20
... Scheme shows the reactions of the two-step mechanism of action of TFPI It is shown in the Appendix that Scheme is equivalent to Scheme I of [8] within the area of the applicability of the latter In ... values d and found the values of the hypothetical reactions separately The model of the inhibitory action of the Xa–TFPI complex on the enzyme–substrate complex (model 3) The reaction of X–VIIa–TF ... significant amount of the Xa–VIIa–TF complex The mathematical model has shown that the analysis of the inhibition curves of the Scheme A development of Scheme by addition of the reaction of the enzyme–substrate...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
Ngày tải lên :
06/03/2014, 19:20
... representative TEM images of mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the ... oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues Particle and Fibre Toxicology 2011 8:20 Submit your next manuscript to BioMed Central and take ... 36.6 We next determined gene expression in different adipose depots in response to PM2.5 exposure, in terms of the expression of BAT-specific and WAT-specific gene profiles by real-time PCR analysis...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... MEF2 Second, overexpression of a-actinin-4 decreases the interaction of MEF2A and HDAC7 Third, knockdown of a-actinin-4 decreases expression of TAF55 Fourth, MEF2C, a-actinin-4 and HDAC7 associate ... growth and theh survival of tumor cells Gelsolin itself interacts with AR LBD via FXXFF and FXXMF motifs and enhances its activity in the presence of androgen The interaction between the N- and ... component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA-binding proteins [4,22]; and (h) actin is a component of PICs and depletion of actin prevents...
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Báo cáo khoa học: Branched chain mechanism of polymerization and ultrastructure of prion protein amyloid fibrils ppt
Ngày tải lên :
07/03/2014, 10:20
... Branched chain mechanism of polymerization consistent with the mechanism of branched-chain reactions Employing the theory of branched-chain reactions will greatly benefit our understanding of the prion ... strong dependence of the reaction rate on the volume or the shape of reaction vessel, the presence of a lag-phase, threshold effects and a strong dependence of the reaction rate on microimpurities ... used for extraction and purification of PrPSc involved sonication, treatment with detergents and, sometimes, freezing and thawing [49–51] All of these steps cause severe fragmentation of fibrils In...
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Báo cáo khoa học: "Corpus-Based Induction of Syntactic Structure: Models of Dependency and Constituency" pdf
Ngày tải lên :
23/03/2014, 19:20
... the performance of the CCM model of Klein and Manning (2002) running off of Penn treebank word classes Again, if we modify the gold standard so as to make determiners the head of NPs, then this ... Carroll and Charniak (1992), Yuret (1998), and Paskin (2002) All systems that we are aware of operate under the assumption that the probability of a dependency structure is the product of the ... of the structure, most often the direction of the dependency (whether the arrow points left or right) Some notation before we present specific models: a dependency d is a pair h, a of a head and...
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TimeML: Robust Specification of Event and Temporal Expressions in Text doc
Ngày tải lên :
30/03/2014, 16:20
... attribute structure of TIMEX, [(eid signalID relType)] Moreover, as the specification of the values for the eventRelType and timeRelType attributes of EVENT and the relType attribute of TIMEX indicates, ... to handling basic tense and aspect features Introduction to TimeML Unlike most previous attempts at event and temporal specification, TimeML separates the representation of event and temporal expressions ... in temporal and event-based reasoning in language and text, particularly as applied to information extraction and reasoning tasks (cf Mani and Wilson, 2000, ACL Workshop on Spatial and Temporal...
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