structure function and properties of the acquired pellicle

... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin ... sheet through the C-terminal loop is conserved Structure of the FMN-binding region The hydrogen bonds and salt bridge that encompass the ribitol moiety and the phosphate group of FeR and M acetivorans...

Ngày tải lên: 07/03/2014, 02:20

... description of recent advances in the understanding of the channel-, motor- and regulatory components of the Tic complex For reference, some of the available knowledge, including the proposed function of ... which anchor the protein in the membrane The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic ... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest...

Ngày tải lên: 07/03/2014, 03:20

... determined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic ... repeat and relative expression of the HD gene We then determined the structure of the mouse CB1 gene and determined that transcription of the CB1 gene was affected in striatal neurons of transgenic ... defining one of the toxic functions of mutant huntingtin The length of the trinucleotide CAG repeat within the HD gene is correlated with the time of symptom onset, rate of disease progression and severity...

Ngày tải lên: 16/03/2014, 18:20

... describe the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are ... on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in the presence of saturating levels of ... for the four substrates shown in the reaction It seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another...

Ngày tải lên: 17/03/2014, 10:20

... trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and those areas of the papers can be eluted to isolate the individual sugars which can then ... catalytic mechanism of TreS, each of the radioactive substrates was incubated with the enzyme in the presence of high concentrations of the unlabeled product, and the formation of radioactive product ... at times up to 22 h Properties of the TreS purified from M smegmatis Effect of time and protein concentration on formation and characterization of the products The conversion of trehalose to maltose...

Ngày tải lên: 23/03/2014, 13:20

... hydroperoxides and aldehydes These may be the primary substrates of the microsomal or membrane-bound GST in the same way as they are the substrates for Se-dependent glutathione peroxidases (the ‘classical’ ... in the amount of GST protein present, a covalent modification of GST that alters its properties, or a change in the mixture of GST isozymes present in the organ to better suit the enzyme for function ... Using the standard curve constructed from the protein standards (Fig 4B), the molecular mass of the Peak GST subunit was determined to be 34.0 kDa (Table 2) Peak showed subunits of 36.8 and 32.6...

Ngày tải lên: 23/03/2014, 15:20

... as control The reaction rate was calculated from the slope of the absorbance curve, using software installed by the manufacturer (Beckman Coulter) The extinction coefficient under the conditions ... using standard conditions described in the text Results are expressed as the percentage of activity with no addition of solvent Standard error for all experiments is less than 5% of the value ... toward medium and long chain esters of pNP Maximal reaction temperature is 55 °C and LipA shows strong activity in the presence of metals, inhibitors, and organic solvents Based on these properties, ...

Ngày tải lên: 23/03/2014, 21:20

... present the results of the methods of preparing and analyzing the specific transfer factor oral preparation in vitro experimental system The data indicated that the methods were credible and the ... pertaining to the range of normal TF (Table and Figure 1, 2) And the ratio of A260 to A280 was up to the criteria of National Health Department on transfer factor Contents of polypeptides and nucleotides ... laboratory evaluation of each TF batch and of its destined recipient is essential in order to define the function and applications of the TF Thus, what this suggests is that the transfer factor...

Ngày tải lên: 11/08/2014, 20:20

... MOLECULAR FUNCTION AND REGULATION OF THE BAX-ASSOCIATING PROTEIN MOAP-1 Fu Nai Yang INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 MOLECULAR FUNCTION AND REGULATION OF THE ... whether the major function of the BH4 domain in mammalian cells is to -17- Introduction mediate the interaction of the pro-survival members of the Bcl-2 family with Apaf-1 still requires further ... ADP and ATP between the mitochondrial matrix and the intermembrane space (IMS) between the outer membrane and the inner membrane The outer membrane separates the mitochondria from cytosol and...

Ngày tải lên: 14/09/2015, 12:54

... extracts The properties of the enzyme purified from T thioparus were therefore studied in an effort to clarify the nature of reaction and to assign a possible role to the enzyme in the oxidation of the ... due to the removal of iron from the enzyme The addition of GSH did not restore the full activity of enzyme Since the 2,2'-dipyridyl concentration of lO -4 M was not inhibitory, this amount of dipyridyl ... in support of the work and Professor H LEES for his interest and encouragement We also acknowledge the help of Dr K RAMLAL,Department of Geology, University of Manitoba for analysis of copper...

Ngày tải lên: 21/12/2016, 10:51

... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position of the ... noting the switch of Leu13 from being part of the exposed hydrophobic groove in the top-top structures to becoming part of the hydrophobic core in the top-bottom structures and assuming the role of ... of 24 structures, the lowest energy and the best (smaller rmsd from the mean) structure of RIIa D/D Each monomer of RIIa D/D consists of a disordered N-terminal segment Fig Superposition of the...

Ngày tải lên: 08/03/2014, 22:20

... structure and properties of K137E and the K137,141E mutant of human HSP22, aiming to provide new information on the structure of sHsp and to shed new light on their role in the development of human ... However, the overall structures of aB-crystallin and HSP27 predicted by these two models are very similar, and the positions of the b-strands correlate well with the corresponding positions of the ... sHsp The primary structure of the a-crystallin domain of human sHsp is very conservative and the loop connecting the b5 and b7 strands is shorter than the corresponding loop connecting the b5 and...

Ngày tải lên: 23/03/2014, 07:20

... is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location of the rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction of the FG loop with the heme and in the position of the B-helix, with Tyr32(B10) closer to the ... with the remainder of the target residues assigned by detection of NOESY residueheme and interresidue cross-peaks solely by the standard Mb fold, and the observation of relaxation effects and/ or...

Ngày tải lên: 23/03/2014, 17:22

... present structures are characterized with local materials, namely, “Tufo of Matera” for the structures of the "Sassi of Matera," and “Stone of Fasano” for the structures of the "Trulli of Alberobello," ... characteristics of the "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites of the "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... rocky church crypt [3], the energetic behavior and the microclimate of different hypogeous structures in the site of the "Sassi of Matera" [4, 5], and the thermal behavior of different opaque envelopes...

Ngày tải lên: 05/09/2013, 14:58

Ngày tải lên: 12/12/2013, 00:15

... smaller The RA and the PA sub-domains had lower correlations with their domains than most of the other domains at baseline, at endpoint, and Psychometric properties of the CHIP-CE also for the change ... the SH sub-domain (item 1: ‘‘How often does your child have a lot of fun?’’ and item 4: ‘‘How often does your child feel happy?’’) The items of the SPS sub-domain Psychometric properties of the ... as well as for the domains and sub-domains Furthermore, the sub-domains were correlated with the domains and the total score, and the domains were correlated with the total score The items/sub-domains/domains...

Ngày tải lên: 12/02/2014, 19:20

... by presenting the pulse of Src activity in the plane of the inactive and active fractions, si and s1 If the duration of the stimulus exceeds the critical value, the trajectory in the (si, s1) ... by number in the plane of the Csk and RPTP concentrations Within these two regions of monostability, there are parameter sets where the QSS dependence of the active Src fraction on the inactive ... transition from the Off state to On state (in the bistability domain) Regardless of the slope, the active Src fractions increase during the Off to On transition, whereas the value of the inactive...

Ngày tải lên: 18/02/2014, 11:20

... demonstrates the inter-connectedness of the low and high latitudes, and the global nature of the Earth’s hydrologic cycle Our case studies provide glimpses of wetland ecosystems, which expanded and ultimately ... archive of the species that grew in and near the wetland If the rate of organic matter accumulation exceeds the rate of decomposition in such a wetland environment, then a temporal component to the ... change because of the resistance of pollens and spores to decay, and their ubiquity and abundance (Traverse 2008) Wetlands are excellent sources of pollen and spores and like macrofossils, palynomorph...

Ngày tải lên: 18/02/2014, 20:20

... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... regions and the entire 3¢ untranslated regions The standard polyadenylation signals (AATAAA) and the poly(A)-tails of different lengths are present in each clone The open reading frames of the five ... comparison of the patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and...

Ngày tải lên: 20/02/2014, 11:20

... c the Soret band was reported to absorb at 418 nm, the b-band at 520 nm and the a–band at 550 nm [18] The deviations of approximately nm between the signals of horse heart cytochrome c and the ... at 415 and 442 nm, and for the oxidized form at 403 and 422 nm The b–band can be seen at 517 nm and the a–band at 547 nm and 603 nm The difference signals that can be observed between 400 and 700 ... work we study the electrochemical, UV-VIS and FT-IR spectroscopic properties of the caa3oxidase from T thermophilus in the presence and absence of cyanide, and compare the observed properties to...

Ngày tải lên: 21/02/2014, 15:20