structure dna replication and virus assembly

... progression, DNA replication, and DNA repair mechanisms When forks collapse during replication and generate single-stranded DNA ends, these ends activate the replication checkpoint Activated replication ... Pathophysiologic Role and Clinical Application DNA replication and MCM2-7 family proteins Prior to DNA replication and during late M and G1 phases of the cell cycle, MCM2-7 form the pre -replication complex ... progression of meiotic DNA replication from DNA replication during mitotic proliferation, connecting DNA replication and homologous recombination Several chapters are dealing with viral DNA replication...

Ngày tải lên: 08/03/2014, 19:20

... generation of excessive single-stranded DNA and allow coordinated and coupled leading and lagging strand synthesis Unlike gp59, the viral gp41 helicase is required for extended replication of R-loop substrates ... leading- and lagging-strand synthesis and in this context has been called a “gatekeeper” When gp59 binds to replication fork-like structures in the absence of gp41, it blocks extension by T4 DNA polymerase ... leads to the DNA arrest phenotype, characterized by a paucity of late DNA replication The additional inactivation of UvsW suppresses this DNA arrest phenotype and allows high levels of DNA synthesis...

Ngày tải lên: 11/08/2014, 21:21

... PCNA, DNA primase, GINS, and MCM Since DNA replication proteins should interact physically and/ or functionally in the replication factory, one can expect that genes encoding different DNA replication ... (that is, these DNA replication factors are heteromultimeric proteins), including RFC (RFC-s and RFC-l), primase (PriS and PriL), the PolD holoenzyme (DP1 and DP2), and Topo VI (A and B subunits) ... annotations and to look for missing proteins (see Materials and methods); Additional data file provides a table list- ing all putative DNA replication proteins identified and used in our analysis DNA replication...

Ngày tải lên: 14/08/2014, 08:21

... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage ... checkpoints, and the G2/M DNA damage checkpoint The G1 and G2 checkpoints respond to some types of localized physical damage to the DNA and are known as DNA damage checkpoints In S.cerevisiae, the G1 DNA ... Regulation of Spindle Dynamics by DNA Replication Checkpoint 183 6.2 Regulation of Spindle Dynamics by the DNA Damage Checkpoint 186 6.3 A Unified View of DNA Replication and Damage Checkpoints ...

Ngày tải lên: 14/09/2015, 14:03

... GROWING DNA POLYMER ENTERING dTTP DNA TEMPLATE dNTP GẮN VÀO CHUỖI DNA ĐANG KÉO DÀI TẠI VỊ TRÍ 3’ Các thành phần tham gia trình nhân đôi DNA dnaA Primasome dnaB dnaC dnaG DNA gyrase Rep protein SSB DNA ... the content of T, and the content of G equals the content of C in double-stranded DNA from any species Mạch đôi DNA 5’ 3’ Rãnh Rãnh phụ “B” DNA 3’ 5’ 3’ 5’ DNA LÀ CỐT LÕI CỦA NST DNA LÀ CỐT LÕI ... III DNA pol I DNA ligase gắn vào trình tự DNA mạch gốc helicase (tháo xoắn DNA sợi gốc) gắn dnaB primase (tổng hợp RNA primer) nhận biết đầu âm cuộn xoắn DNA ngã nhân đôi helicase (tháo xoắn DNA...

Ngày tải lên: 09/11/2016, 04:25

... and with various batches of the template DNAs pJC389 and pJC433 ([32]; Figs and 2) As expected murine DNA polymerase a-primase did not support SV40 DNA replication (Fig 2A, lanes and 6) JCV DNA ... SV40 and JCV DNA replication using the monopolymerase system The SV40 system contains SV40 TAg and DNA with the SV40 replication origin (A); the JCV system contains JCV TAg and DNA with the JCV replication ... of JCV DNA replication on human replication proteins Figure represents a comparison between the SV40 (panel A) and JCV (panel B) cell-free DNA replication systems Figure 1B shows a DNA replication...

Ngày tải lên: 17/03/2014, 03:20

... we investigated protein DNA interactions involving the RuvA and RuvC proteins, and have analysed both quantitative and qualitative differences between wild-type RuvA and a mutant which lacks ... comprised four 24-mer and four 50-mer oligonucleotides DNA annealing and purification were essentially as described previously [33] HJ50 contained HJ5, 6, and HJ 25 contained HJ1, 2, and Three way junctions ... 25 contained HJ1, 2, and Three way junctions were prepared using HJ5, and and duplex DNA annealed using D1 and D2, ASP1 and ASP2 All oligonucleotides used in the binding studies are shown in...

Ngày tải lên: 21/02/2014, 01:21

... chromatin assembly factors and remodelers participate in the rebuilding of higher-order chromatin after DNA replication Perhaps some chromatin assembly factors are specialized for early replication and ... play during DNA replication and the passage of DNA polymerases and helicases In light of this, it is interesting to note that the FACT components Spt16 and Pob3 are found associated with DNA polymerase ... remodelers, thus improving access of replication factors to the origin Replication- coupled chromatin assembly Once replication initiation has been triggered and as the replication fork plows through...

Ngày tải lên: 16/03/2014, 16:20

... constructs were assembled by standard cloning methods and confirmed by DNA sequencing The backbone of pEBTet stems from pCEP-Pu [23] The Tn10-derived TetR open reading frame and the CMV-tetO2-tetO2 promoter ... underlined) cDNAs were inserted into the polylinkers of pEBTet or pEBTetD The cDNA sequence of ETTh [9] corresponds to GenBank entry Y09881 For pEBTet ⁄ ETTh, the 5¢ interface between cDNA and pEBTet ... transporter probe and with a GAPDH probe (cf Fig 2) Relative background transcription was calculated from the ratios of signals for transporter mRNA and GAPDH mRNA With OCT1, OCT2, and CAT4 both...

Ngày tải lên: 23/03/2014, 09:21

... both their anti-tumour activity and DNAbinding properties, providing unambiguous evidence of a marked structure activity relation Materials and methods Drugs and DNA A series of actinomycin derivatives ... the melting of DNA and its complexes with ligands may be calculated from the general thermodynamic relation: DG ¼ DH À TDS ð4Þ The thermostabilities of DNA and its complexes with ligands were investigated ... of DNA and its complexes with drugs, calculated using eqns 2–4 and the binding parameters, DHbind, DSbind and DGbind, are summarized in Table Differences in interaction of ActII–ActV with DNA...

Ngày tải lên: 31/03/2014, 07:20

... AG/A site and internal AG/K site mutations, the N-terminal AG/A and C-terminal AG/K site mutations, and the internal and C-terminal AG/K site mutations were indicated as SD 1&2, SD 1&3, and SD 2&3, ... nuclei and insoluble fraction was resuspended in 900 μl of PBS [15] Abbreviations VV: Vaccinia virus; IV: Immature virus; IMV: Intracellular mature virus; IEV: Intracellular enveloped virus; ... processing of vaccinia virus membrane and core components J Virol 2004, 78:6335-6343 Mercer J, Traktman P: Genetic and cell biological characterization of the vaccinia virus A30 and G7 phosphoproteins...

Ngày tải lên: 18/06/2014, 18:20

... VP0, VP1 and VP3, which form a noncovalent complex, the pro- Genomic structure of poliovirus and processing of the P3 domain of the polyprotein Figure Genomic structure of poliovirus and processing ... 42 43 Gamarnik AV, Andino R: Switch from translation to RNA replication in a positive strand RNA virus Genes Dev 1998, 12(15):2293-2304 Herold J, Andino R: Poliovirus RNA replication requires ... stimulation by 3CDpro both during RNA synthesis and particle assembly remains to be determined Materials and methods Cells and viruses HeLa R19 cell monolayers and suspension cultures of HeLa S3 cells...

Ngày tải lên: 19/06/2014, 08:20

... AG/A site and internal AG/K site mutations, the N-terminal AG/A and C-terminal AG/K site mutations, and the internal and C-terminal AG/K site mutations were indicated as SD 1&2, SD 1&3, and SD 2&3, ... nuclei and insoluble fraction was resuspended in 900 μl of PBS [15] Abbreviations VV: Vaccinia virus; IV: Immature virus; IMV: Intracellular mature virus; IEV: Intracellular enveloped virus; ... processing of vaccinia virus membrane and core components J Virol 2004, 78:6335-6343 Mercer J, Traktman P: Genetic and cell biological characterization of the vaccinia virus A30 and G7 phosphoproteins...

Ngày tải lên: 20/06/2014, 01:20

... pRB-1B5 DNA; Lane – pRB-1B*6X DNA; Lane – vRB-1B5-infected CEF DNA; Lane – vRB-1B*6X-infected CEF DNA; Lane – wtRB-1Binfected CEF DNA C: Comparison of in vitro and in vivo replication and percentage ... loxP site in the virus genome The cre/loxP system has been used on BAC clones of other herpesviruses such as pseudorabies virus [16], human cytomegalovirus [17] and rhesus cytomegalovirus [18] to ... (black triangle) and wtRB-1B (grey diamond) viruses The survival rates showed statistically significant differences between the wtRB-1B and vRB1B5 (p =< 0.0001) and wtRB-1B virus and vRB-1B*X6 groups...

Ngày tải lên: 20/06/2014, 01:20

... JJ: Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus J Virol 1974, 13:394-399 Cook RF, Avery J, Dimmock NJ: Infection of chicken erythrocytes with influenza and ... STAT1, and PKZ at regular intervals during haemagglutination reactions using native virus and virus inactivated by exposure either to UV light or to heat The data are summarized in Figures and The ... (elution and uptake) of the ISAV-induced haemagglutination of Atlantic salmon erythrocytes between virus strains of differing pathogenicities We found remarkable differences in virus replication and...

Ngày tải lên: 20/06/2014, 01:20

... hepatitis B and C viruses [6] Moreover, point mutations in Coxsackie virus B3 (CVB3/0) and influenza A virus (H3N2) have been associated with increased disease severity in Se-deficient mice [7-9], and ... immunodeficiency virus type replication in vitro AIDS Res Hum Retroviruses 1997, 13(15):1325-1332 Sandstrom PA, Murray J, Folks TM, Diamond AM: Antioxidant defenses influence HIV-1 replication and associated ... Se-deficient and Se-adequate Vero and SK-N-SH cells and the profile of antioxidant enzymes, such as CuZnSOD, MnSOD, catalase and iNOS, were characterized by Western blotting at days and 10 post-induction...

Ngày tải lên: 20/06/2014, 01:20

... and PRRSV, respectively Assessment of Replication and Virulence of Attenuated Pseudorabies Virus in Swine 63 Viru s iso latio n an d qu a n titatio n The presence and level of PRV in swabs and ... and respiratory syndrome virus (PRRSV) based on serology Additionally, the day before challenge, a blood sample and nasal swab were collected from each animal and tested for PRV by serology and ... I (Fig 3) to contrast the PRV MS+1 and the viruses reisolated after back-passage, did not revealed any changes in the number and pattern of DNA fragments of viruses reisolated from tissues in...

Ngày tải lên: 07/08/2014, 15:20

... interactions of bacteriophage T4 DNA replication proteins gp59 and gp32 with singlestranded (ss) DNA Co-modulation of ssDNA binding activities in a DNA helicase assembly intermediate J Biol Chem ... Bacteriophage T4 DNA Replication The semi-conservative, semi-discontinuous process of DNA replication is conserved in all life forms The parental anti-parallel DNA strands are separated and copied ... which promotes DNA unwinding and the subsequent bi-directional loading of DnaB, the replicative helicase [5] Assembly of the replication complex and synthesis of an RNA primer by DnaG initiates...

Ngày tải lên: 11/08/2014, 21:21

... factor and inhibit IFN induction when transferred into A/Udorn/1/1972 (H3N2) virus [32] Here we show that the F103L and M106I NS1 gene mutations are adaptive and control IAV replication and virulence ... infected with the parental recombinant viruses rHK-wt, rPR8-wt and rWSN-wt and the different recombinant viruses, rPR8HK-NS, rPR8-H5N1-NS and rWSN-Ck/Bj-NS viruses at MOI = Twenty-four hours post ... and Ile at position 106 were selected in mice and independently in avian IAV lineages and are required for an increase in virulence and growth in both human A/HK/1/68 and avian (Ck/ Bj/1/95 and...

Ngày tải lên: 11/08/2014, 21:21