... determined the sequence andstructureofthe 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced thestructureofthe CB1 gene by comparing the cDNA sequences to the genomic ... repeat and relative expression ofthe HD gene We then determined thestructureofthe mouse CB1 gene and determined that transcription ofthe CB1 gene was affected in striatal neurons of transgenic ... levels in the medial striatum of any ofthe wild-type and HD mice examined The optical density ofthe hybridization signal in the lateral striatum, corrected by subtracting the signal in the medial...
... In the case of non-related lemmas, one of them is usually much more frequent (as with avto and avt), whereas in the case of related lemmas, the meaning ofthe missing one can be inferred from the ... match a text string as a part ofthe dictionary entry; match a text string at the beginning ofthe dictionary entry In the current version, the internal structureofthe dictionary is a table containing ... Slovenian, the text is spell-checked and proof-read, the error-rate is low (Jakopin, 2002) The results of our evaluation will give an approximation of how well the lexical knowledge represented in the...
... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions ofthe v-KIND KIND2 andthe MAP2 CD were ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members ofthe KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) ofthe endogenous MAP2 from cerebellar lysates of P21 ... downstream of v-KIND overexpression [12]) enhances the activity of MAP2 to bind to microtubules and promote their assembly [18], and that the inhibition of JNK1 (such as downstream of v-KIND knockdown...
... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment ofthe HcA clone to the major sequence ofthe upper hemocyanin band in the SDS/PAGE ... regions andthe entire 3¢ untranslated regions The standard polyadenylation signals (AATAAA) andthe poly(A)-tails of different lengths are present in each clone The open reading frames ofthe five ... comparison ofthe patterns ofthe SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and...
... for the determination ofthe structures ofthe conformers ofthe C-domain The dipolar couplings provided long-distance information on the global folding of both conformers Thestructureofthe ... on one side ofthe b-sheet and two on the other side The rmsd ofthe heavy atoms (Ca, C, and N) ofthe protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal ofthe HN of Gly337 in the open conformer andthe signal ofthe water protons All of these observations indicate that the loop region ofthe open conformer has a higher degree of...
... Resolution ofthestructureof NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side ofthe isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... present the crystal structureof DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing ofthe avoredoxin ... sheet through the C-terminal loop is conserved Structureofthe FMN-binding region The hydrogen bonds and salt bridge that encompass the ribitol moiety andthe phosphate group of FeR and M acetivorans...
... comparison of part ofthe protein backbone structureofthe representative solution structureofthe human eRF1 M domain andthe Ca trace in the crystal structureof RF1 in the whole ribosome structure ... on the equivalent segments of each member ofthe family ofthe solution structureofthe M domain of human eRF1 The resulting rmsd values (y-axis) and their deviations through the 25 NMR structures ... Fig The solution structures ofthe M domain of human eRF1 (A) The stereo view ofthe ensemble ofthe final 25 calculated structures superimposed on heavy backbone atoms (Ca, N and C) The poorly structured...
... involved in binding ofthe cofactor ThDP are located at the C-terminal ends ofthe b-strands of Dom-c (diphosphates and Mg2+) andof Dom-a¢ of a neighbouring subunit (pyrimidine moiety) The active centre ... with the gene ofthe benzaldehyde lyase and for helpful discussions, the teams ofthe Swiss Light Source (Villigen ⁄ CH) andofthe EMBL outstation Hamburg for their help with data collection and ... comparison ofthe relative B-factors along the ThDP molecules shows that the Fig Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis The secondary structure...
... appropriate subroutines This raises the further question ofthe functions and design ofthe executive routine and subroutines In this type of parsing program, the function ofthe executive routine will ... view the executive routine ofthe algorithm which contains the grammar as the actual parsing algorithm and to view the remaining portions as forms of input data Leaving aside the matters of rule ... difference in the adequacy ofthe parsings of natural-language sentences will be alterations ofthe format itself in terms of increasing the degree of context-sensitivity This in effect means that the...
... CHAPTER – THESTRUCTUREAND DEVELOPMENT OFTHE CEREAL PLANT 24 THE WHEAT BOOK THE WHEAT BOOK CHAPTER – THESTRUCTUREAND DEVELOPMENT OFTHE CEREAL PLANT THESTRUCTUREAND DEVELOPMENT OFTHE CEREAL ... parts of a leaf are the tip ofthe blade andthe top ofthe sheath Where the blade and sheath join, there are structures called the ligule andthe auricles (Figure 2.3) Characteristics ofthe shoot ... structureofthe stem and leaf ofthe wheat plant 27 CHAPTER – THESTRUCTUREAND DEVELOPMENT OFTHE CEREAL PLANT DESCRIPTION OFTHE WHEAT PLANT THE WHEAT BOOK (continued) at their bases, so the...
... represent the NMR structures The quality ofthe final structures was accessed using the program of PROCHECK-NMR [35] Further analysis and visualization ofthe conformers including calculation of root ... structures of DMT1-TM4 in both SDS (pH 6.0) and TFE were chosen to represent the solution structures ofthe peptide, as shown in Fig The quality ofthe final structures was assessed using the program ... disappearance ofthe cross-peaks of Ala13 in the TOCSY spectrum (Fig 6B), a significant reduction in intensities ofthe cross-peaks of Leu9 and Phe16, and a moderate reduction in intensities ofthe cross-...
... to other proteins of this class, such as the DNA-binding domain ofthe Mu repressor [21] Third, the backbone atoms ofthe region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... N-terminal, structured part of Xis is involved in the recognition of DNA [18,57] On the other hand, the results also indicate that the C-terminal a-helix, predicted in the work of Wu and co-authors for ... 12–17 (the flexible loop between the two a-helices), these structures can be superposed with a backbone RMSD ˚ of 0.71 A The statistics ofthe final structure calculation are summarized in Table The...
... [86] Nevertheless, the observed homodimerization ofthe GCD andthe metal ion dependence ofthe catalytic activity support the integrity ofthe expressed proteins As expected from the high sequence ... site in the AC C1 domain [81] The N-terminal and C-terminal ends of each monomer are labeled The two-fold symmetry axis in the dimer runs perpendicular to the plane ofthe page The dimer structure ... lmolÆmin)1Æmg)1 to 23 lmolÆmin)1Æmg)1 [29,88–90] Together, these data seem to suggest that thestructureofthe Cyg12 GCD dimer may reflect thestructureofthe GCD in the dimerized full-length NPRA in its basal...
... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structureofthe protein and therefore the ... in the ArCMLE1 structureThe figure was created from the coordinates ofthe P212121 structureof ArCMLE1 andof PpCMLE (Protein Data Bank code 1RE5) (B) Water cavity below the active site The ... competitive 0.98 and 121 versus noncompetitive 0.99 and 58 Site-directed mutagenesis of ArCMLE1 From the crystal structureofthe P putida CMLE [6] and sequence comparisons with other members ofthe fumarase...
... LaRonde-LeBlanc et al Structureand activity ofthe Rio1 kinase Fig Thestructureand conservation of Rio1 (A) Thestructureof APO-Rio1 showing the important kinase domain features andthe Rio1specific ... Asp99 and Phe102, Met104 and Ile107, and Ser108 and Glu111, which marks the start of aC The entire flexible loop packs between the N-terminal portion of aC and part ofthe C-lobe (Figs 2A and 3A) The ... Stereoview ofthe alignment ofthe active sites of AfRio1 (green) and AfRio2 (orange; PDB code 1ZAO) (D) Stereoview ofthe alignment ofthe active sites and bound nuceotide of AfRio1 (green) and PKA...
... Southern analysis ofthe zebrafish fabp3 gene DNA sequence andstructureofthe zebrafish fabp3 gene Using a pair of primers flanking the entire coding region and a portion ofthe 3¢ UTR for the zebrafish ... sequences ofthe cDNA andthe 5¢ upstream region of zebrafish fabp3 gene (A) The 5¢ upstream region of zebrafish fabp3 gene The coding sequence ofthe first exon is shown in uppercase letters and underlined ... migrated off the end ofthe gel during electrophoresis As both the HaeIII and MboIdigested DNA samples generated two fragments in the Southern blot hybridization, and neither site is present in the...
... position ofthe chemical shifts of proton H-4 and carbon C-4 ofthe Kdo-residue (C) of compound andthe downfield shift to the same frequencies of proton H-5 and carbon C-5 ofthe Kdo-residue (C) of ... linked to the a-GlcN (A) ofthe lipid A backbone leading to a splitting ofthe signal of its anomeric proton and another phosphate linked to the 4-position ofthe b-configured GlcN (B) The far downfield ... glucosamine backbone ofthe LPS of H influenzae I-69 Although, Kdo-4P alone is not bound to the antibody, the position ofthe phosphate group strictly determines the specificity ofthe epitope as no...
... very indicative, in our opinion, ofthe adequacy andthe basis of this orientation, on the one hand, andthe destiny of linguistics, on the other hand Of course, these implications might be too ... constructions The frequency of WA, FA-, LA-, and Ø in front of QAD Frequency ofthe compound perfect The distribution and frequency of sawfa/saThe frequency of QAD, laʕalla, and rubbamaa The contextual ... STRUCTUREAND FUNCTION OFTHE ARABIC VERB Structureand Function ofthe Arabic Verb is a corpus-based study that unveils the morpho-syntax andthe semantics ofthe Arabic verb...
... learnability and suitability, influences the level of acceptance and use of mRDTs The effectiveness ofthe health system in providing the enabling environment andthe integration ofthediagnostic ... of performance, readability ofthe results, cost, heat stability data, and reported sensitivity and specificity The accuracy ofthe ICT Pf brand had earlier been established in Uganda [15], and ... Ministry of Health of Uganda, andthe Uganda National Council of Science and Technology (UNCST) The procedures followed were in accordance with the Helsinki Declaration In addition to asking the HC...