structure contents and location of the automatic diagnostic repository

... determined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic ... repeat and relative expression of the HD gene We then determined the structure of the mouse CB1 gene and determined that transcription of the CB1 gene was affected in striatal neurons of transgenic ... levels in the medial striatum of any of the wild-type and HD mice examined The optical density of the hybridization signal in the lateral striatum, corrected by subtracting the signal in the medial...

Ngày tải lên: 16/03/2014, 18:20

... In the case of non-related lemmas, one of them is usually much more frequent (as with avto and avt), whereas in the case of related lemmas, the meaning of the missing one can be inferred from the ... match a text string as a part of the dictionary entry; match a text string at the beginning of the dictionary entry In the current version, the internal structure of the dictionary is a table containing ... Slovenian, the text is spell-checked and proof-read, the error-rate is low (Jakopin, 2002) The results of our evaluation will give an approximation of how well the lexical knowledge represented in the...

Ngày tải lên: 17/03/2014, 22:20

Ngày tải lên: 12/12/2013, 00:15

... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21 ... downstream of v-KIND overexpression [12]) enhances the activity of MAP2 to bind to microtubules and promote their assembly [18], and that the inhibition of JNK1 (such as downstream of v-KIND knockdown...

Ngày tải lên: 14/02/2014, 19:20

... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... regions and the entire 3¢ untranslated regions The standard polyadenylation signals (AATAAA) and the poly(A)-tails of different lengths are present in each clone The open reading frames of the five ... comparison of the patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and...

Ngày tải lên: 20/02/2014, 11:20

... for the determination of the structures of the conformers of the C-domain The dipolar couplings provided long-distance information on the global folding of both conformers The structure of the ... on one side of the b-sheet and two on the other side The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal of the HN of Gly337 in the open conformer and the signal of the water protons All of these observations indicate that the loop region of the open conformer has a higher degree of...

Ngày tải lên: 06/03/2014, 11:20

... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin ... sheet through the C-terminal loop is conserved Structure of the FMN-binding region The hydrogen bonds and salt bridge that encompass the ribitol moiety and the phosphate group of FeR and M acetivorans...

Ngày tải lên: 07/03/2014, 02:20

... comparison of part of the protein backbone structure of the representative solution structure of the human eRF1 M domain and the Ca trace in the crystal structure of RF1 in the whole ribosome structure ... on the equivalent segments of each member of the family of the solution structure of the M domain of human eRF1 The resulting rmsd values (y-axis) and their deviations through the 25 NMR structures ... Fig The solution structures of the M domain of human eRF1 (A) The stereo view of the ensemble of the final 25 calculated structures superimposed on heavy backbone atoms (Ca, N and C) The poorly structured...

Ngày tải lên: 07/03/2014, 05:20

... involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg2+) and of Dom-a¢ of a neighbouring subunit (pyrimidine moiety) The active centre ... with the gene of the benzaldehyde lyase and for helpful discussions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... comparison of the relative B-factors along the ThDP molecules shows that the Fig Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis The secondary structure...

Ngày tải lên: 07/03/2014, 12:20

... appropriate subroutines This raises the further question of the functions and design of the executive routine and subroutines In this type of parsing program, the function of the executive routine will ... view the executive routine of the algorithm which contains the grammar as the actual parsing algorithm and to view the remaining portions as forms of input data Leaving aside the matters of rule ... difference in the adequacy of the parsings of natural-language sentences will be alterations of the format itself in terms of increasing the degree of context-sensitivity This in effect means that the...

Ngày tải lên: 07/03/2014, 18:20

... CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT 24 THE WHEAT BOOK THE WHEAT BOOK CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT THE STRUCTURE AND DEVELOPMENT OF THE CEREAL ... parts of a leaf are the tip of the blade and the top of the sheath Where the blade and sheath join, there are structures called the ligule and the auricles (Figure 2.3) Characteristics of the shoot ... structure of the stem and leaf of the wheat plant 27 CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT DESCRIPTION OF THE WHEAT PLANT THE WHEAT BOOK (continued) at their bases, so the...

Ngày tải lên: 08/03/2014, 23:20

... represent the NMR structures The quality of the final structures was accessed using the program of PROCHECK-NMR [35] Further analysis and visualization of the conformers including calculation of root ... structures of DMT1-TM4 in both SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig The quality of the final structures was assessed using the program ... disappearance of the cross-peaks of Ala13 in the TOCSY spectrum (Fig 6B), a significant reduction in intensities of the cross-peaks of Leu9 and Phe16, and a moderate reduction in intensities of the cross-...

Ngày tải lên: 16/03/2014, 16:20

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21] Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... N-terminal, structured part of Xis is involved in the recognition of DNA [18,57] On the other hand, the results also indicate that the C-terminal a-helix, predicted in the work of Wu and co-authors for ... 12–17 (the flexible loop between the two a-helices), these structures can be superposed with a backbone RMSD ˚ of 0.71 A The statistics of the final structure calculation are summarized in Table The...

Ngày tải lên: 23/03/2014, 15:21

... [86] Nevertheless, the observed homodimerization of the GCD and the metal ion dependence of the catalytic activity support the integrity of the expressed proteins As expected from the high sequence ... site in the AC C1 domain [81] The N-terminal and C-terminal ends of each monomer are labeled The two-fold symmetry axis in the dimer runs perpendicular to the plane of the page The dimer structure ... lmolÆmin)1Æmg)1 to 23 lmolÆmin)1Æmg)1 [29,88–90] Together, these data seem to suggest that the structure of the Cyg12 GCD dimer may reflect the structure of the GCD in the dimerized full-length NPRA in its basal...

Ngày tải lên: 28/03/2014, 23:20

... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... in the ArCMLE1 structure The figure was created from the coordinates of the P212121 structure of ArCMLE1 and of PpCMLE (Protein Data Bank code 1RE5) (B) Water cavity below the active site The ... competitive 0.98 and 121 versus noncompetitive 0.99 and 58 Site-directed mutagenesis of ArCMLE1 From the crystal structure of the P putida CMLE [6] and sequence comparisons with other members of the fumarase...

Ngày tải lên: 30/03/2014, 10:20

... LaRonde-LeBlanc et al Structure and activity of the Rio1 kinase Fig The structure and conservation of Rio1 (A) The structure of APO-Rio1 showing the important kinase domain features and the Rio1specific ... Asp99 and Phe102, Met104 and Ile107, and Ser108 and Glu111, which marks the start of aC The entire flexible loop packs between the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A) The ... Stereoview of the alignment of the active sites of AfRio1 (green) and AfRio2 (orange; PDB code 1ZAO) (D) Stereoview of the alignment of the active sites and bound nuceotide of AfRio1 (green) and PKA...

Ngày tải lên: 30/03/2014, 20:20

... Southern analysis of the zebrafish fabp3 gene DNA sequence and structure of the zebrafish fabp3 gene Using a pair of primers flanking the entire coding region and a portion of the 3¢ UTR for the zebrafish ... sequences of the cDNA and the 5¢ upstream region of zebrafish fabp3 gene (A) The 5¢ upstream region of zebrafish fabp3 gene The coding sequence of the first exon is shown in uppercase letters and underlined ... migrated off the end of the gel during electrophoresis As both the HaeIII and MboIdigested DNA samples generated two fragments in the Southern blot hybridization, and neither site is present in the...

Ngày tải lên: 31/03/2014, 07:20

... position of the chemical shifts of proton H-4 and carbon C-4 of the Kdo-residue (C) of compound and the downfield shift to the same frequencies of proton H-5 and carbon C-5 of the Kdo-residue (C) of ... linked to the a-GlcN (A) of the lipid A backbone leading to a splitting of the signal of its anomeric proton and another phosphate linked to the 4-position of the b-configured GlcN (B) The far downfield ... glucosamine backbone of the LPS of H influenzae I-69 Although, Kdo-4P alone is not bound to the antibody, the position of the phosphate group strictly determines the specificity of the epitope as no...

Ngày tải lên: 31/03/2014, 21:21

... very indicative, in our opinion, of the adequacy and the basis of this orientation, on the one hand, and the destiny of linguistics, on the other hand Of course, these implications might be too ... constructions The frequency of WA, FA-, LA-, and Ø in front of QAD Frequency of the compound perfect The distribution and frequency of sawfa/saThe frequency of QAD, laʕalla, and rubbamaa The contextual ... STRUCTURE AND FUNCTION OF THE ARABIC VERB Structure and Function of the Arabic Verb is a corpus-based study that unveils the morpho-syntax and the semantics of the Arabic verb...

Ngày tải lên: 03/04/2014, 12:48

... learnability and suitability, influences the level of acceptance and use of mRDTs The effectiveness of the health system in providing the enabling environment and the integration of the diagnostic ... of performance, readability of the results, cost, heat stability data, and reported sensitivity and specificity The accuracy of the ICT Pf brand had earlier been established in Uganda [15], and ... Ministry of Health of Uganda, and the Uganda National Council of Science and Technology (UNCST) The procedures followed were in accordance with the Helsinki Declaration In addition to asking the HC...

Ngày tải lên: 21/06/2014, 19:20