structural characterization of mammalian selenoproteins

... level of the third codon of PfPNP gene Comparison of the deduced primary sequence of PfPNP with enzymes present in GenBank Data Base reveals a much higher similarity of PfPNP with members of MTAP ... pocket where His223 of PfPNP substitutes Ala215 of PfMTAP Also in this case, the same substitution takes place in mammalian enzyme where the change of His257 of hPNP with Val233 of hMTAP makes hydrophilic ... environmental conditions Characterization of C254S ⁄ C256S mutant and role of the CXC motif To elucidate if the disulfide CGC localized at the C-terminus of PfPNP, in spite of its unusual structural features,...

Ngày tải lên: 23/03/2014, 09:21

... region of VpU, as indicated by a small number of observed long-range NOEs [24] NMR structure of micelle-associated HIV-1 VpUcyt Another important aspect is the topology of the cytoplasmic domain of ... the expected number of cross-peaks of VpUcyt were obtained with perdeuterated DPC (DPC-d38) at 30 °C A series of HSQC spectra of mm VpUcyt was recorded with varying amounts of DPC-d38 in the sample ... mark the theoretical average values of the downfield shift of 13Ca (top) and 13CO (bottom) as well as of the upfield shift of 1Ha resonances (middle) characteristic of a 100% helical secondary structure...

Ngày tải lên: 18/02/2014, 06:20

... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... effect of the d-Phe on structure, was also studied The unusual structure of conomarphin further demonstrates the high diversity of conotoxins Results Purification and primary sequence of conomarphin...

Ngày tải lên: 18/02/2014, 17:20

... others A structural alignment of these three models shows that they are indeed close structural Table The quality of fits (described by the chi-squared per degrees of freedom) for a series of Ca2+ ... 1.2 molar ratio of Ca2+ ⁄ CaM to PhK5 had been reached The inset shows an expanded view of the indicated area of the spectrum (Bottom) An overlay of spectra from the titration of Ca2+ ⁄ CaM with ... hand motifs of each CaM domain The presence of a relatively large number of Ca2+⁄ CaM ⁄ peptide complexes in the PDB opens up the possibility of using these structures to perform structural comparisons...

Ngày tải lên: 19/02/2014, 17:20

... Ó FEBS 2003 Minor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in b a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2 experiments) ... to the phosphate group at O-4 of Kdo [11,13,16] Structure of the core region of the Hep3-glycoforms In the 1H NMR spectrum of OS-1 (Fig 5A), anomeric resonances of HepI–HepIII were observed at ... Biochem 270) Preparation of OS material O-Deacylation of LPS O-Deacylation of LPS was achieved with anhydrous hydrazine, as described previously [16,26] Fig Structural model of the lipopolysaccharide...

Ngày tải lên: 08/03/2014, 02:21

... details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, which consists of only ... number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression studies may help in the prediction of the severity of HPA ... expression of the p.Q301P mutant enzyme was decreased As shown by western blotting (Fig 1A), in the presence of antiPAH serum, the intensity of the band corresponding to the 50 kDa monomeric form of...

Ngày tải lên: 16/03/2014, 01:20

... graphic softwares [22] Fig NMR characterization of Nogo-24 NH-NH region of a NOESY spectrum of Nogo-24 (mixing time of 250 ms) acquired in an aqueous buffer (50 mM phosphate buffer at pH of 6.5) ... region of a NOESY spectrum (mixing time of 250 ms) of Nogo-40 acquired in a 50 mM phosphate buffer (pH 6.5) at 15 °C (D) NH-aliphatic region of a NOESY spectrum of Nogo-40 (mixing time of 250 ... NMR characterization of the Nogo-A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation of the domain organization of the human Nogo-A protein (A) The domain organization of...

Ngày tải lên: 16/03/2014, 16:20

... large lumenal loop of the CP47 instead of the 33 kDa protein These results suggest that the structural patterns of the OEC proteins differs and not form basic structural feature of the PS II core ... peak of PSII A small shoulder at the front edge of the main peak of PSII represents the PSI contaminant Samples of PSII complexes for electron microscopy were collected from the maximum of the ... significant reduction of a mass in upper part of the particle was observed (Fig 6D) The presence of millimolar concentration of divalent ions in the buffer induced the artificial aggregation of two single...

Ngày tải lên: 16/03/2014, 18:20

... appendages of the bacterium, a hypothesis requiring further investigation It is of note that the structure of the LPS O-antigen of F columnare differs structurally from the LPS O-antigen of the fish ... a polymer of regular trisaccharide repeating units composed of three aminoglycose residues The chemical shift assignments in the 1H-NMR and 13 C-NMR spectra and the characterization of the glycose ... correlation of H2C to the C2C at 55.4 p.p.m is consistent with the presence of a C2 acetamido substituent, and the lack of a proton at C6, considered in conjunction with the long-range correlation of...

Ngày tải lên: 17/03/2014, 10:20

... evidence of the solubility limits of Sn4þ in the hematite structure and of Fe3þ in SnO2 are discussed the compositional uniformity The determined average values of x; for the analyzed series of mixed ... with coordinates of ð0; 0; zÞ occupy 2/3 of the octahedral holes in successive oxygen layers, and 1/3 of the octahedral holes with coordinates of ð0; 0; 0Þ are empty In the case of our samples, ... understanding of the site occupancy of Sn4þ in the hematite lattice [12] Finally, it was found that the degree of order given by the mentioned approach is far from perfect and that the microstructural...

Ngày tải lên: 19/03/2014, 16:48

... one of the most difficult challenges and often requires the application of multiple analytical approaches [8] MS has become a powerful analytical tool for the structural characterization of oligosaccharides ... Knowledge about the nature of oligosaccharides present in the glycoproteins is crucial for better understanding of its functional aspects [7] However, structural characterization of oligosaccharides ... characteristic of laminin Composition analysis of laminin was carried out in terms of total sugars, amino sugars and sialic acid (Table 2) The monosaccharide composition of oligosaccharides of laminin...

Ngày tải lên: 22/03/2014, 17:20

... Fig Structural comparisons of the funnels of LGOX, LAAO, and PAO, and the residues composing two entrances of the funnel of LGOX (A) Stereo view of funnels of LAAO, PAO, and LGOX The surfaces of ... Through comparison of the enzymes’ funnel shapes, the cavity of the active site (space around the N5 of FAD) of LGOX was found to be narrower than that of LAAO A structural study of PAO also revealed ... surface of the protein into the interior, terminating at the active site near to the FAD cofactor The presence of the long funnel is a charcteristic of a number of flavoenzymes The funnel of LGOX...

Ngày tải lên: 23/03/2014, 05:22

... of b sheet [7,8] Higher resolution studies of the structure of PrPC have made use of NMR and X-ray crystallography methods, but have focused almost entirely on analysis of recombinant forms of ... removed Fig MS characterization and HPLC separation of refolded states of PrP–S231C (A) Electrospray MS and deconvoluted MS (inset) of PrP-Glut after refolding of PrP–S231C in the presence of glutathione ... PrP could adopt a wide range of orientations relative to the plane of the cell membrane Some of these orientations would allow the possibility of direct interactions of the protein with the membrane...

Ngày tải lên: 23/03/2014, 10:21

... possibility of functional dissection of the dynamin protein into specific domains suggests that a detailed characterization of the intrinsic structural and dynamic characteristics of the individual ... on the interactions of the individual domains, and the mechanism and variety of the overall functions of the full length protein As of now, the structural characteristics of only the pleckstrin ... propensity for formation of short helices, transiently, and the chain as such is highly dynamic Structure of the core of the oligomers Because of the large size of the core of the oligomer, the NMR...

Ngày tải lên: 23/03/2014, 11:20

... the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this large ... IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band (indicated ... isolation of large amounts of pure Ndh complex from maize chloroplasts for further structural and functional studies This may include identification of further Ndh subunits, as well as determination of...

Ngày tải lên: 23/03/2014, 13:20

... excitation of H-1 H-resonance of the GlcNAc residue in the TOCSY step and of H-3/H-4 1H-resonance of the GlcNAc residue in the NOESY step (A) The assignments of the 1H-resonances of the Gal II ... The structure of the oligosaccharide chain of the sialylated glycoforms was determined from a series of selective excitation NMR experiments [27] Initially the axial resonance of the sialic acid ... sialylated glycoforms of the O-deacylated LPS of H influenzae strain RM118 and mutants causing sequential truncations from Hep III are illustrated in Fig DISCUSSION Structural analysis of H influenzae...

Ngày tải lên: 23/03/2014, 21:21

... temperature program of 120–180 °C at °C/min and 180–250 °C at °C/min) Mass spectra were obtained at an ion energy of 70 eV, a current intensity of 500 lA and temperature of 250 °C 2.15 Statistical ... residues, with a degree of branching (DB) of 0.33 Discussion Fig Helix-coil transition analysis of CPMN Fr III and standard polymers according to the absorption maximum of the Congo red–polysaccharide ... complex at various concentrations of NaOH For more details, see Section Fig Identification of the anomeric configuration of CPMN Fr III and standard polymers Visualization of b-linked polysaccharides...

Ngày tải lên: 28/06/2014, 11:20

... data represent the average of three independent sets of experiments with each set of experiments performed twice D Structural classification of compounds A series of compounds that independently ... Characterization of CB29 binding .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterization of CB7 binding 123 D Probing CB7 binding of ALDH3A1 site ... mechanism of conversion of aldehydes to carboxylic acid by ALDH3A1 in the presence of NAD(P)+ and water Important aldehyde dehydrogenase family members Structural, kinetic and knockout studies of several...

Ngày tải lên: 24/08/2014, 09:51

... Discussion 91 V 3.3 Structural Characterization of Nogo-B and Systematic Dissection of the Am-Nogo-A 92 3.3.1 Bioinformatics analysis 93 3.3.2 CD and NMR characterization of N- and C-termini of Nogo-B ... Structural Characterization of NgBR 134 3.6.1 Cloning and expression of NgBR and its dissected domains 136 3.6.2 Bioinformatics characterization 138 3.6.3 Structural characterization of the NgBR ... Secondary structural prediction of Nogo-40 by DNAMAN 61 Figure 3.1.5 NMR characterization of Nogo-24 64 Figure 3.1.6 CD and NMR characterization of Nogo-40 65 Figure 3.1.7 NMR spectral assignment of...

Ngày tải lên: 14/09/2015, 12:53

... fraction of the (311) reflection of magnetite Figure 4d shows a part of the wu¨stite nanocrystals by selecting a fraction of the (220) reflection of wu¨stite On the basis of the observation of diffuse ... particles of 14 nm and spherical particles of nm obtained by decomposition of mmol Fe(OAc)2 in 12 mmol of OA/14 mL of TOA (c) Cubic FexO particle isolated in the early growth state (8 mmol of Fe(OAc)2 ... precipitations of diluted hexane solutions with an equal volume of acetone Decomposition of Iron Pentacarbonyl in the Presence of Pyridine N-Oxide In a typical reaction, 7.6 mmol of PyO and 3.02 mmol of...

Ngày tải lên: 01/12/2016, 16:32