structural analysis and evaluation

... analysis and drafted the manuscript XM and LC participated in its design and coordination and helped to draft the manuscript GH and ZR participated in its design and experimental work LZ participated ... functions of the ORFs As shown in Fig and 10, the secondary structure of gp17 protein was composed of 33.10% Alpha helix (Hh), 25.25% Extended strand (Ee) and 30.29% Random coil (Cc) The PHYRE program ... nodes helices and b strands with intermittent unstructured intervening regions The arrangement is characteristic of crystallized hydrolase Domain B is composed of a helix and b strands with intermittent...

Ngày tải lên: 12/08/2014, 01:22

... terms "cough" and "environment" a total of 984 entries were found in PubMed While 370 entries contained the terms "cough" and "smoke" (Fig 1), 306 entries contained "cough" and "tobacco" and 298 entries ... ETS exposure [60] Figure search for the terms cough and tobacco and publication dates PubMed2 PubMed search for the terms cough and tobacco and publication dates An exponential trendline indicates ... nonsmoking wives and smoking discordant husbands Here, it was also shown that wives with never-smoked husbands had lower frequencies of chronic cough, next to a better socio-economic status and better...

Ngày tải lên: 13/08/2014, 08:20

... Concepts and Skills • Understand forecasting risk and sources of value • Understand and be able to conduct scenario and sensitivity analysis • Understand the various forms of break-even analysis ... is sensitivity analysis, scenario analysis and simulation? • Why are these analyses important, and how should they be used? • What are the three types of break-even analysis, and how should each ... 66,400 39,357 19.7% 11-9 Simulation Analysis • Simulation is really just an expanded sensitivity and scenario analysis • Monte Carlo simulation can estimate thousands of possible outcomes based on...

Ngày tải lên: 25/03/2015, 19:44

... Background Electrolyte and Detection Mode 52 2.2.5 Extraction of Nodularin and Microcystins LR, RR and YR 52 2.3 Results and discussion 53 2.3.1 Detector and Background Electrolyte ... involve relatively lengthy analysis time and high solvent and chemical reagent consumption More sensitive than mouse bioassay, and can distinguish the microcystins if standards are available Using ... effects of microcystins LR, RR and YR were studied because they are the most frequently occurring microcystins (in Poland 4, 5,Thailand and in Europe; in Africa, Australia and in China ) The effects...

Ngày tải lên: 11/09/2015, 10:14

... system (Wang and Li, 2003) 11 1.3 Structural analyses of the two focused systems 1.3.1 Analysis types and methods A complete structural analysis typically consists of three types of analysis: static, ... systems and can be found in many real structures The other one is deployable cable-strut truss representing free standing systems and having great potential for quick and temporary use Structural analysis ... vibration and forced vibration analysis Static analysis is to obtain the stress and deformation of the system under static load Free vibration analysis is used to calculate the natural frequencies and...

Ngày tải lên: 14/09/2015, 12:50

... other competitors and new players Figure 13: Figure 11: Figure 12: FINANCIAL ANALYSIS Huge gain in revenue in 2010 and 2012 In 2012, Vingroup recorded a huge gain in revenue in 2010 and 2012 In 2010, ... expand its business by focusing on developing Vinpearl and Vincharm and Vinmec Secondly, In years later, Vingroup’s revenue still based on sale of inventory, including apartments for sale and ... 2013 and 2014 In the two years, the group’s revenue mainly comes from large number of sale and purchase contracts Royal City (operate in July, 2013), Times Thirdly, the demand for medical and...

Ngày tải lên: 19/03/2017, 22:39

... Da and an isoelectric point of 4.81 A search in GenBank revealed 86% and 67% sequence identity between the C sepium b-amylase and the b-amylase from sweet potato and soybean, respectively Analysis ... surfaces was 1.4 A and a standard ˚ Stern layer was used to exclude ions from the 2.0 A molecular surface [37] The inner and outer dielectric constants applied to the protein and the solvent were, ... Concentrations of glucose, maltose and cyclohexaamylose were 80 mM , 80 mM and 1.25 mM , respectively The substrate concentration ranged between 0.025 and 0.5% (w/v) Fig SDS/PAGE and isoelectric focusing...

Ngày tải lên: 22/02/2014, 07:20

... from CONICET and ´ ´ Agencia Nacional de Promocion Cientı´ fica y Tecnologica M F Drincovich and C S Andreo are members of the Researcher Career of CONICET, and V A Campos-Bermudez and F P Bologna ... equilibrated with 100 mm phosphate buffer at pH 7.4, and calibrated using molecular mass standards (Sigma-Aldrich, St Louis, MO, USA) The sample and the standards were applied separately in a final volume ... Pta and the three truncated Ptas in both the forward and reverse reactions (Fig 5A) NADH and ATP substantially inhibited the activity of E coli Pta in both directions (Fig 5A) On the other hand,...

Ngày tải lên: 06/03/2014, 11:20

... allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic ... glycopeptide with a signal at m ⁄ z 1608.7 obtained by PSD analysis amidase-F and glycoamidase-A were permethylated and subjected to MALDI MS and MS ⁄ MS analyses, taking advantage of the enzyme’s ... a-(1,3)-fucosylated, and a small portion also carries core xylosylation and ⁄ or additional GlcNAc This pattern of heterogeneity was demonstrated by MS analysis of both the glycopeptides and the released...

Ngày tải lên: 07/03/2014, 12:20

... (residues G and I), a-Galp (residues K and L), and a-GlcpN (A) The analysis of an HMBC spectrum showing intraresidual cross-correlation signals from anomeric protons to carbons C-3 and C-5 was ... at the reducing end, residues A and B) and at positions of both L,D-Hepp-residues E and F as determined by 31P,1H-COSY, as well as 1H- and 13C-chemical shift analysis The sequence of residues ... L,D-Hepp (residue H) and the inner a-Kdop (C), and between the adjacent L,D-Hepp (residue F) and the side-chain a-Kdop (residue D) were observed in the analysis of the R3 and R4 core oligosaccharides...

Ngày tải lên: 08/03/2014, 09:20

... DNA polymerase (Toyobo, Japan), using the bF and 68r primers, bR and 68f primers, aF and 109r primers, aR and 109f primers, aF and 114r primers, or aR and 114f primers Following an initial denaturation ... 55 °C, and a incubation at 68 °C The second PCR was also performed with KOD-plus DNA polymerase, using the two megaprimers and the bF and bR primers, or the two megaprimers and the aF and aR ... reaction, was used The 0.72 kb DNA fragment and pUC18-NHase were digested with EcoRI and NotI, and the 1.08 kb DNA fragment and pUC18-NHase were digested with NotI and HindIII The PCR product was ligated...

Ngày tải lên: 16/03/2014, 16:20

... Protein Data Bank IDs 2D8D, 1YBZ and 1ECM 2D8D and 1YBZ are annotated as CMs from Thermus thermophilus (TtCM) and Pyrococcus furiosus (PfCM), respectively, from Structural Genomics projects on ... structural and sequence homology with EcCM, the active site residues of 90-MtCM can be identified, and are shown in Fig The striking difference between EcCM and 90MtCM is that the EcCM residues Ser84 and ... (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such as Bacillus subtilis (BsCM) [18]’ was corrected to ‘Erwinia herbicola CM (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such...

Ngày tải lên: 17/03/2014, 17:20

... manner, and incorporating alignment-based features into the CCG parser (Clark and Curran, 2007) is also a viable possibility some of the variables ( j and k in rules (i) and (iii), and j, k, and ... NP-COOD and VP-COOD Among coordinations annotated with COOD tags, we selected those surrounding and, ” “or,” and “but.” This yielded 3598 coordinations (2997, 355, and 246 for and, ” “or,” and “but,” ... semantic similarity calculated from a large text collection, and agreement of numbers between n1 and n2 and between n1 and n3 Nakov and Hearst (2005) collected web-based 968 COORD COORD CJT N...

Ngày tải lên: 23/03/2014, 16:21

... and passive methods require an understanding of their mechanism and control For this reason a major part of the book is devoted to the damping of structural vibrations Structural Vibration: Analysis ... structural vibrations Structural Vibration: Analysis and Damping benefits from my earlier book Structural Vibration Analysis: Modelling, Analysis and Damping of Vibrating Structures which was published ... engineers, designers and students of engineering to first degree Preface vii level a thorough understanding of the principles involved in the analysis of structural vibration and to provide a sound...

Ngày tải lên: 28/05/2014, 20:06

... Figure 1.12 Structural Analysis: In Theory and Practice 12 loads at joint 3, and the internal forces acting on it from the right-hand portion of the structure Similarly, the right-hand portion ... fundamentals of structural analysis, which I believe are becoming lost in structural engineering Having a solid foundation in the fundamentals of analysis enables engineers to understand the behavior ... better understanding of structural analysis; it will make you more proficient and efficient in your day-to-day work David A Fanella, Ph.D., S.E., P.E Chicago, IL March 2008 Part One Analysis of...

Ngày tải lên: 28/05/2014, 20:15

... HPLC Analysis for Evaluation 128 plasticizers and other soluble components may leach out over extended periods, while water or saliva is absorbed The loss of plasticizer may cause brittleness and ... hardness and the weight percentages of the insoluble parts The mean domain size and the volume fraction of the rubber phase favor that of the water-bath cured specimens Naik and Jabade4 evaluated and ... switched off and left for 15 It was then boiled again for 20 and then switched off let it to cool down slowly Specimen was taken out from mould and the excess margins were polished by the sandpaper...

Ngày tải lên: 07/08/2014, 14:20

... spine imaging and cryomicrotomy Spine 2001, 26:2443-2448 Antonaci F, Bulgheroni M, Ghirmai S, Lanfranchi S, Dalla Toffola E, Sandrini G, Nappi G: 3D kinematic analysis and clinical evaluation of ... highly automated function and therefore better suited for standardized analysis A clinically validated marker system [27,28] was adopted for the purpose of this investigation, and the consistency of ... citation purposes) Journal of Brachial Plexus and Peripheral Nerve Injury 2009, 4:10 gait and posture abnormalities ;and (2) Computerized 3D gait analysis provides objective data for diagnosis...

Ngày tải lên: 10/08/2014, 10:20

... DNA strands are separated and copied following hydrogen bonding rules for the keto form of each base as proposed by Watson and Crick [1] Progeny cells therefore inherit one parental strand and one ... 5’ to 3’ exonuclease, and gp30 ligase, the ATP dependent DNA ligase Leading and lagging strand synthesis is coordinated by the replisome Lagging strand primer extension and helicase progression ... both leading and lagging strand DNA synthesis The Pol B family includes eukaryotic pol a, δ, and ε The full length T4 enzyme and the exo- mutant (D219A) have been cloned, expressed and purified...

Ngày tải lên: 11/08/2014, 21:21

... incandescent lamps (OSRAM, Germany) Seeds and seedlings were watered six days a week and given a complete nutrient solution once a week Plant tissue preparation and examination For structural analysis, ... provide valuable insights into elucidating and understanding the biochemical and structural basis of the abscission process [26] Such mutants have been described and characterized in a wide range of ... harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis and the drafting of the manuscript MA...

Ngày tải lên: 12/08/2014, 03:20

... tables and graphics in these pages summarize all known modifications from different experiments, along with relevant literature and information about the number and type of sequence and structural ... source The database is publicly available online, and experimentalists are encouraged to submit their data for storage and display Results Handling and storage of phosphosite data The mtcPTM database ... structures (see Materials and methods, below) The NR set comprised 324 structural models, representing a wide range of Pfam domains, and contained 264 modified serine/threonine and 219 tyrosine residues...

Ngày tải lên: 14/08/2014, 07:21