stress protein misfolding synaptic injury and neuronal cell death
Báo cáo " RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS AND ANIMAL CELL TECHNOLOGY " potx
Ngày tải lên :
28/03/2014, 09:20
... media and sufficient oxygen supply (Sandig et al., 2005) Animal cells have low cell density and grow at slow kinetics (Sandig et al., 2005) They are also high sensitive mechanical stress (Sandig ... CHO cell lines, efficiency of protein production is dependent on the fraction of cells carrying transgene in a functional and nonrearranged form Continuous growth in culture, cell handling, and ... disadvantages and challenges which could suffer from when using animal cell in the production of recombinant proteins Due to low yield and complicated and costly cultivation, therefore animal cells...
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Báo cáo khoa học: The silencing of adenine nucleotide translocase isoform 1 induces oxidative stress and programmed cell death in ADF human glioblastoma cells doc
Ngày tải lên :
29/03/2014, 09:20
... Scrambleand ANT1 and ANT2 siRNA-transfected cells; the 33 kDa band corresponds to ANT proteins, the 43 kDa band corresponds to actin (E) Western blot analysis of FLAGANT1 and FLAG-ANT2 recombinant protein ... ANT1 and ANT2-silencing on ADF cells (A) Cell growth curves analyzed by crystal violet assay in nontransfected ADF cells, and in Scramble- and ANT1 siRNA and ANT2 siRNA ADF-transfected cells ... BA, ATR and ANT1 siRNA on cell viability and glucose consumption (A,B) Cell growth curves analyzed by crystal violet assay in BA and vehicle-treated (A) and in ATR and vehicle-treated (B) cells...
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ecombinant protein production expression systems and animal cell technology
Ngày tải lên :
28/06/2014, 12:52
... media and sufficient oxygen supply (Sandig et al., 2005) • Animal cells have low cell density and grow at slow kinetics (Sandig et al., 2005) • They are also high sensitive mechanical stress (Sandig ... CHO cell lines, efficiency of protein production is dependent on the fraction of cells carrying transgene in a functional and nonrearranged form Continuous growth in culture, cell handling, and ... disadvantages and challenges which could suffer from when using animal cell in the production of recombinant proteins • Due to low yield and complicated and costly cultivation, therefore animal cells...
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Dual functions of AP 1 in neuronal cell death and differentiation
Ngày tải lên :
16/09/2015, 15:54
... nitric oxide on some important cellular proteins 19 1.2.4 Understanding the paradoxical effects of nitric oxide on cell viability 23 1.3 AP-1 and programmed cell death 26 1.3.1 Regulation ... 1.1 Programmed cell death Programmed cell death (PCD) is an evolutionary conserved process that is important for multicellular organisms to delete unwanted cells during development and homeostasis ... necrosis, a pathological form of cell death resulting from acute cellular injury, is characterized by cell swelling and lysis, release of cytoplasmic contents, and the induction of an inflammatory...
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Báo cáo khoa học: " Hepatitis C virus core protein induces apoptosis-like caspase independent cell death" docx
Ngày tải lên :
12/08/2014, 04:21
... HCV proteins and protein complexes did not show any effect with the exception of a very slight stimulation by the NS3-4A and NS4B proteins Cell death induction of the core protein expressing cells ... the core protein and not on the cell density employed Thus, testing two high and two low expression cell lines from the UHCV and the UC cells, DNA fragmentation was induced only in the UC cell line ... core protein (data not shown) Cell death could not be induced by further HCV proteins Next, we addressed the question, whether further HCV proteins expressed in our test system also exert cell death...
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Mechanisms of hypochlorous acid mediated neuronal cell death
Ngày tải lên :
10/11/2015, 12:35
... which cells play an active role in their own death Apoptosis describes cell death morphologically by cell shrinkage, plasma condensation, and membrane blebbing, followed by cell fragmentation and ... processes between h and 24 h treatment 85 Figure 5.3 Protein expression of ATF-3, HSP70, and cell cycle proteins analyzed by Western blotting 86 Figure 5.4 Effect of HOCl on cellular GSH and ATP content ... activation and calpain inhibitors provide protection against HOCl-induced cell death 67 4.4 Intracellular Ca2+ levels are increased in HOCl-treated cortical neurons 69 4.5 Extracellular and intracellular...
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Báo cáo khoa học: Shedding of the amyloid precursor protein-like protein APLP2 by disintegrin-metalloproteinases Retinoic acid-induced upregulation of substrate and proteinase ADAM10 during neuronal cell differentiation ppt
Ngày tải lên :
30/03/2014, 11:20
... inhibitor of matrixmetalloproteinases (MMPs) and ADAMs, decreased basal APPsa and APLP2s secretion to 60 and 75%, respectively, of untreated cells (Fig 2A,B, lanes and 3) and reduced the PMA-stimulated ... neuroblastoma cell lines on ADAM10 and TACE expression and on the release of secreted APLP2 and APPsa For neuronal (N)-type SH-SY5Y cells, differentiation by RA was accompanied by the generation of long cellular ... Expression of APLP2 (A) and ADAM10 (B) in differentiated neuroblastoma cell lines Cell lysates of RA-differentiated SH-SY5Y and SKNMC cells were subjected to 7.5% SDS ⁄ PAGE, and the proteins were detected...
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báo cáo khoa học: " The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathway" pptx
Ngày tải lên :
11/08/2014, 11:21
... the ER stress- and osmotic stress- integrating Figure The osmotic -stress and ER -stress signal-integrating pathway ER stress and osmotic stress activate two independent signaling pathways (1 and 2), ... potentiate a cell death response Therefore, the integration of the ER stress and osmotic stress signals into a circuit of cell death occurs through the activation of NRPmediated signaling This cell death ... target of the NRP-mediated cell death signaling that integrates ER stress and osmotic stress responses GmNAC6 promotes cell death in tobacco leaves and in soybean cells We have recently demonstrated...
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Báo cáo y học: " Classic swine fever virus NS2 protein leads to the induction of cell cycle arrest at S-phase and endoplasmic reticulum stress" potx
Ngày tải lên :
12/08/2014, 04:21
... the subcellular localization of CSFV NS2 protein and elucidate the effects and mechanisms of this protein on cell growth and cell cycle progression Here, we show that the expression of NS2 protein ... CSFV NS2 protein has properties that can alter cell cycle replication Presently, no data exists on the subcellular localization of CSFV NS2 protein and its effects on cell growth and cell cycle ... examine the expression and subcellular localization of CSFV NS2 protein, the stable cell lines expressing GFP-NS2, NS2-GFP protein or control cells (GFP and untransfected cells) were grown on glass...
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Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
Ngày tải lên :
19/02/2014, 05:20
... both HeLa and NIH ⁄ 3T3 cells (Fig 4A–C) In the same cells the mitogen-activated protein kinase or extracellular signal-regulated kinase (MEK1) protein fused to a C-terminal EGFP tag and expressed ... the nurse cells of the ovaries and in the preblastoderm embryos [1] After gastrulation, the maternal transcript disappears and the zygotic transcript and protein are found in a subset of cells expressing ... localizations of the human ER and PDIP46/SKAR proteins We examined the intracellular localization of the human ER and PDIP46 ⁄ SKAR proteins fused with enhanced green fluorescent protein (EGFP) in the...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... bromophenol blue and 30 mM dithiothreitol) and then incubated at 86 °C for Typically, cell extracts (5–10 lg of protein) were separated by SDS/PAGE using the standard Laemmli system [30] with 13% and 4% ... of mM DNP on cell growth (h) and expression of UP12 (j) (B) Monitoring of cell growth (s) and amount of UP12 (d) before and after addition of 0.1 mM CCCP (C) Effect of temperature on cell growth...
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Báo cáo khoa học: Protein-misfolding diseases and chaperone-based therapeutic approaches pdf
Ngày tải lên :
07/03/2014, 12:20
... the cell, and the use of different chaperones as therapeutic agents against various protein- misfolding disorders Protein misfolding and aggregation cause several diseases Protein misfolding and ... Protein- misfolding diseases T K Chaudhuri and S Paul and non-native states of targeted proteins, but how they discriminate between correctly and incorrectly folded proteins and how they ... gene-encoded protein ataxin-3 The truncated form of mutated ataxin-3 causes aggregation and cell death in vitro and in vivo In vitro cellular models and transgenic animals have been created and 1336...
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Báo cáo khoa học: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling docx
Ngày tải lên :
07/03/2014, 12:20
... specific subcellular locations where they bind to regulatory and ⁄ or substrate proteins and are integrated into cell signalling pathways and cell cycle events [23] The UD, Src homology and Src homology ... The proteins were visualized by silver staining, and the protein bands were analysed by MALDI-TOF MS Protein identification by peptide mass fingerprinting analysis Silver stained candidate bands ... vivo in 253J cells and in cotransfected 293T cells (A) To confirm whether MVP interacts endogenously with full-length Src, we prepared 253J (stomach cancer cell line) cell lysates and immunoprecipitated...
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Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt
Ngày tải lên :
16/03/2014, 11:20
... plasmids are shown in (B) and (E) on SD plus L-histidine and adenine and (C) and (F) on SD lacking L-histidine and adenine forming activity of Rat1 cells expressing both exogenous proteins was examined ... and HAUXT fusion proteins are indicated by arrows bacterially expressed and 35S-methionine-labelled proteins and the results are shown in Fig These confirm that UXT binds Rp (Fig 3B, lane 5) and ... Recombinant retrovirus produced in BOSC-23 cells was used to infect Rat1 and RatFL cells and cell populations expressing UXT were selected Neither UXT expression in Rat1 cells nor empty vector controls...
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Báo cáo khoa học: Hyperefficient PrPSc amplification of mouse-adapted BSE and scrapie strain by protein misfolding cyclic amplification technique pptx
Ngày tải lên :
23/03/2014, 04:21
... (ng) C PK (–) PK (+) NBH mBSE Chandler Fig Estimation of PrPSc concentration in mBSE-BH and Chandler-BH by dot-blot analysis (A) Detection of PrP in NBH, mBSEBH and Chandler-BH without ()) or with ... PK and peptide:N-glycosidase F (PNGase F), a single 16 kDa band of nonglycosylated PrPres(NBH) was detected; the fragments of nonglycosylated mBSE- and ChandlerPrPSc were estimated to be 17 and ... PrPSc amplification A Fujihara et al (mM) 37 id az ol e Im TA A ED mBSE-BH and Chandler-BH were 1.21 and 1.86 lgÆmg)1 of total protein, respectively When conventional PMCA is performed on BHs, EDTA...
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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
Ngày tải lên :
23/03/2014, 10:21
... the protein in stress- responsive pathways Developing cell models to examine effects of DCA on cell death SEP53 was originally identified as a protein synthesized ex vivo after heat or ethanol stress ... detected (lanes and versus and 4) In the middle panel, SEP53 expression vectors were used in HCT116 cells without a tag (lanes and 6), with a HIS-tag (lanes and 7), or GST tag (lanes and 8) and immunoblotted ... antibodies to SEP53 and the human SEP53 gene was cloned into a bacterial and insect cell- expression system for the purification and acquisition of full-length protein for immunization, and to develop...
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Báo cáo khoa học: Human papillomavirus 16 E7 protein inhibits interferon-c-mediated enhancement of keratinocyte antigen processing and T-cell lysis docx
Ngày tải lên :
29/03/2014, 00:20
... immunotherapy [32,33] IFN-c, secreted by CD8 T cells, by activated NK and NKT cells and by dendritic cells [34,35], is a key intermediate mediator of CD8 T-effector cell function, enhancing antigen presentation ... and E7-KCs to IFN-c and SIINFEKL, a peptide A B C D Fig Lysis of KCs and E7-KCs treated with IFN-c Mouse KCs were exposed to SIINFEKL and IFN-c as shown (A) Expression of MHC class I on KCs and ... soluble protein into the class I pathway of antigen processing and presentation Cell 54, 777–785 Carbone FR & Bevan MJ (1990) Class I-restricted processing and presentation of exogenous cell- associated...
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Báo cáo khoa học: ERK and cell death: ERK1⁄2 in neuronal death Srinivasa Subramaniam1 and Klaus Unsicker2 pptx
Ngày tải lên :
29/03/2014, 08:20
... hand, in non -neuronal cells, cytoplasmic retention of ERK1 ⁄ is required for death- associated protein kinase-mediated cell death [35] Thus, ERK1 ⁄ might promote cell death depending upon the cell ... ERK1 ⁄ 2-implicated cell death paradigms ERK1 ⁄ in cellular models of neuronal death The first evidence for a role of ERK1 ⁄ in cell death was demonstrated in the oligodendroglial cell line, CG4 The ... 2-mediated neuronal death are only beginning to emerge It is challenging to understand how ERK1 ⁄ can promote either neuronal survival or neuronal death under different paradigms Oxidative stress...
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Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death ppt
Ngày tải lên :
29/03/2014, 08:20
... shown to participate in cell death in response to various cytokine signals, including IFN-cinduced cell death [2], TNF-a and FAS-induced cell death [48], and TGF-b-induced cell death [10] There are ... control and cell metabolism [15] In mammalian cells, two structurally and functionally distinct mTOR-containing complexes have been identified, mTORC1 and mTORC2 [15] mTORC1 directly regulates cell ... source maintaining cell survival, it has been proposed that autophagy can contribute to cell death in a process DAPK and signal transduction termed autophagic (type II) cell death Disturbance...
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Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death docx
Ngày tải lên :
29/03/2014, 08:20
... blebbing cells has not been examined in blebs of cells undergoing cell death Although the proteins involved in the execution of blebbing appear similar in apoptotic and autophagic cell death, the ... apoptotic cell death resemble those of ‘healthy’ cells Growth and retraction FEBS Journal 277 (2010) 58–65 ª 2009 The Authors Journal compilation ª 2009 FEBS 61 Blebbing in programmed cell death ... recruitment of membrane–cortex linker proteins, actin, actin-bundling proteins and contractile proteins Indeed, as bleb expansion slows, the ERM protein ezrin (and possibly moesin) is rapidly recruited...
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