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... CELL SELECTION AND RESELECTION TTKTVT HCM NGUYỄN VĂN CƯỜNG VIETTEL CORPORATION CELL SELECTION AND RESELECTION TTKTVT HCM NGUYỄN VĂN CƯỜNG VIETTEL CORPORATION TTKTVT HCM CELL SELECTION AND RESELECTION ... MEASUMENTS FOR CELL SELECTION MS search tất kênh hệ thống (124 for P‑GSM, 174 for E‑GSM, 194 for R-GSM, and 374 for DCS 1 800) MS đọc sóng mang tính toán cường độ trung bình sóng trị trung bình tính toán ... VĂN CƯỜNG VIETTEL CORPORATION TTKTVT HCM CELL SELECTION AND RESELECTION THE END CELL SELECTION AND RESELECTION NGUYỄN VĂN CƯỜNG ...

Ngày tải lên: 11/03/2014, 09:20

... ginseng culture has continued to attract considerable research and development effort in recent years and scientists seek to understand and optimize the culture conditions [2] As earlier reported ... increase of the growth ratio and dry weight were not accompanied by an increase of the saponin content Using MS medium minus NH4NO3 and plus 0.5% glucose and 2% sucrose and 2% sucrose added after ... Sciences and Technology 23 (2007) 269-274 Determination and analyses Extraction and determination of ginsenoside production were determined as reported previously [5,6] Experimental design and data...

Ngày tải lên: 12/02/2014, 10:20

... suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of ginseng cell culture Materials and methods Stock cell culture and culture condition Stock ... claimed to require 2,4-D which is a potent herbicide and carcinogen and therefore unsuitable for pharmaceutical and food industries [10] From this point of view, our system is apparently favourable ... 215–224 Wu J.Y and J.J Zhong 1999 Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects J Biotech., 68: 89-99 Dornenburg H and D Knorr...

Ngày tải lên: 14/03/2014, 10:20

... (chemostat) and substrate saturation (pH-auxostat) The specic cellular glucose and oxygen consumption rates, together with the specic protein and carbon dioxide production rates (qglu, qO2 , qCO2 and ... dilution rate was and remained 100% Both datasets indicate that no products other than biomass and CO2 were synthesized, conrming that the specic ows of catabolism (qCO2 and qO2 ) and anabolism (lmax) ... Henk van Verseveld and R J Planta We thank Bas Kooijman (Theoretical Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, The Netherlands) for discussions and advice This study...

Ngày tải lên: 16/03/2014, 04:20

... conditions were done as described by [9] Results and discussion Effects of macro elements on biomass and ginsenoside production Table and Figure show how growth and yield of ginseng cell were affected ... Higher strengths (1.0, 1.5, and 2.0) of MgSO4 were more favorable for both cell biomass growth and ginsenoside accumulation, as seen by the highest cell biomass DW (10.4 g) and ginsenoside content ... grants from the Department of Science and Technology, Vietnam National University Hanoi (QG.06.14), and Basic Research Program in Life Sciences, Ministry of Science and Technology (6.090.06) to Hanoi...

Ngày tải lên: 28/03/2014, 10:20

... thymocytes, positive and negative selecting ligands induce the localization of the components of the Ras ⁄ ERK cascade and active ERK into distinct subcellular compartments (Figs and 2) [16] Several ... negative selecting ligand Note the separate location of pJNK and pERK, and Ras-GRP1 ⁄ Grb2 ⁄ SOS ⁄ Ras ⁄ Raf at the plasma membrane lead to such distinct cell fates as survival and death Much is ... LAT They only activate Ras-GRP1 and induce its recruitment to the Golgi [16] However, the fact that negative selecting ligands activate and recruit both Ras-GRP1 and Grb2 ⁄ SOS to the plasma membrane...

Ngày tải lên: 29/03/2014, 08:20

... 0.1% hyaluronidase (Sigma), and 0.01% DNAse (Sigma) The resultant single-cell suspension was plated onto culture dishes and maintained in DMEM (Gibco BLR, Grand Island, NY, USA) containing 10 ... streptomycin, and 10% heat-inactivated FCS (Hyclone Laboratories, Logan, UT, USA) After four to five passages, leukocytes and macrophages were removed from the culture and only the adherent, and apparently ... CD29, CD49e, CD44, and CD54, and only weakly positive for CD106, but were negative for CD11a, CD18, and CD49d (Fig 3) RA-SNC77 cells also expressed a CS-1 isoform of fibronectin and a chemokine...

Ngày tải lên: 09/08/2014, 06:22

... structures and, eventually, the destruction and replacement of the affected glandular tissue [8,27-35] Page of 12 (page number not for citation purposes) Notably, cytokine-mediate and/ or autoantibody-mediated ... B-cell-targeted cytokines and survival factors, including BAFF and APRIL (a proliferation-inducing ligand) [50,55] Thus, a tight interplay between activated SGECs, infiltrating lymphocytes and DCs leads ... different types of exocrine gland [66] In patients with pSS and recurrent or persistent swelling of the major salivary glands, the lymphocytic lesions of the involved glands often contain massive...

Ngày tải lên: 09/08/2014, 10:20

... (18, 44, 46, and 48) and palindromic (1, 43, 45, and 47) INH-ODNs lacking the CCT element (45 and 46), GGG element (43 and 44), or both (47 and 48) Similar to control ODN 4173, INH-47 and INH-48 ... to complex TLR ligands [1318] This enhancement may be due to multiple mechanisms (for example, TLR7 and upregulation, increased ligand uptake, BCR-mediated delivery of TLR ligands to 'autophagosomes' ... INH-43, INH-45, and INH-47 are palindromic variants of INH-1 in which the CCT and/ or GGG elements were replaced with random nucleotide triplets Similarly, ODNs INH-44, INH-46, and INH-48 are linear...

Ngày tải lên: 09/08/2014, 14:21

... both transmembrane and soluble isoforms and is critical for ligand binding and signal transduction through the receptor [27,28] Negative regulators of IFN and other proinflammatory cytokine signaling, ... metric and complete linkage method was applied, and results were depicted as a heatmap and dendogram generated using Java Treeview software [36] A full list of antigens included on the array and ... containing 3% FBS and 0.05% Tween-20 followed by isotype-specific HRP-conjugated GAM-Ig (SouthernBiotech) Standard curves were constructed using mouse Ig isotype standards (SouthernBiotech), and total...

Ngày tải lên: 09/08/2014, 14:22

... on 2N cells, and lack of information about the ecophysiology and biochemistry of 1N cells represents a large knowledge gap in understanding the biology and evolution of E huxleyi and coccolithophores ... ribosomal structure and biogenesis Carbohydrate transport and metabolism Energy production and conversion Intracell traffic., secretion and vesicular transport Amino acid transport and metabolism ... Replication, recombination, and repair Coenzyme transport and metabolism Chromatin structure and dynamics Nucleotide transport and metabolism Cell cycle control, division and chromosome partition...

Ngày tải lên: 09/08/2014, 20:20

... especially Cd and Pb) and riverbank and sand hill reinforcement [7] However, these qualities also contribute to its competitive advantage over other plants and result in monospecific stands, which ... statistics (F-values and degrees of freedom) and several other plant characteristics, such as stem, branch and leaf numbers, leaf area, SLA, stem and leaf water contents and carbon content, reflecting ... species/clones on the production of stilbenes and emodin? Results The biomass and chemical characteristics measured and tested by ANOVA are shown in Table F-values and Page of 14 degrees of freedom may...

Ngày tải lên: 11/08/2014, 11:20

... contributions YTZ and HJX designed this study HJX and GHZ carried out the experiments JPM, ZXD, SYL, and JBH coordinated and performed the primate studies YTZ, HJX, and GHZ analyzed the results and drafted ... day 76 and increased Finally, the number declined and remained at a low level because of the death of 00317 and 98081 (Figure 3A) The mean count of mDC reached the highest site on days 202 and 265, ... 728.3 pg/ml; range, 13 to 3591 pg/ml), and then recovered and remained at a subnormal level until monkeys 98081 and 00079 lost the ability for IFN-a production and died of AIDS (mean, 201 pg/ml;...

Ngày tải lên: 13/08/2014, 01:20

... (0.7 ml) and sterile sand (0.25 g: Sigma -50+70 mesh; Sigma, Gillingham, Dorset, UK), and vortexed vigorously for 90 s to shear genomic DNA and homogenize the sample Cell debris and sand were ... Materials and methods and those referred to in the text are noted in Additional data file reports Illustration of (b) M570 (relA- sampling on cultures of (a) M600 (relA+ Figure and the growth and ppGpp-) ... the organism and the amino acid In C glutamicum, both the histidine and serine biosynthetic genes are under strong positive stringent control [18], and the his operon in E coli and Salmonella...

Ngày tải lên: 14/08/2014, 08:20

... to pursue PhD and offering me the scholarship And last but not least, this dissertation is dedicated to my family My parents are always standing with me and giving me endless love and encouragement ... for validating and comparing hNESPCs morphology and functional activity in the cells from NP and healthy controls, and demonstrated that the intrinsic factors related to cell growth and differentiation ... and encouragement My husband is always showing his patience and thoughtfulness to my work, and teaching me how to simplify works by using software Without their help and support, it’s hard for...

Ngày tải lên: 09/09/2015, 11:29

... groups for companionship, support and helpful discussions Most importantly, my family has given me incredible support and encouragement Their love and understanding helped me overcome the obstacles ... an incredible environment to grow and nurture; my sister was amazingly supportive, understanding and a great companion I also thank my fiancé for his patience and companionship, who made long ... co-bound and co-regulated by OCT4, SOX2 and NANOG have been identified (Figure 1.1A) In addition, genes that are co-bound by OCT4, NANOG and SOX2   1  include those that promote pluripotency and self-renewal,...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... the minus strand, and overlaps with another lncRNA transcribed from the plus strand (C) lncRNA_ES3 is possibly alternatively spliced, giving rise to two lncRNAs shown: BC026300 and BC018008   ... the nuclear and cytoplasmic fractions, and qPCR was performed to compute nuclear/cytoplasmic ratio HOTAIR and KCNQ1OT1 are well-characterized, nuclear-localized RNAs, while GAPDH and ACTB are ... formaldehyde, and immunoprecipitated with antibodies specific to SUZ12, a component of the PRC2 complex, and pluripotency transcription factors SOX2 and OCT4 The results indicated that lncRNA_ES1 and lncRNA_ES2...

Ngày tải lên: 09/09/2015, 17:54

... hnRNPA2B1 and SOX2 as protein partners of the lncRNA Two additional protein bands corresponding to 37 kDa and 35 kDa were observed in the RMST pulldown, and not in the controls (beads only and antisenseRMST, ... steps were performed and proteins were eventually eluted, ran on an SDS-PAGE gel The SDS-PAGE gel was Coomassie-stained, and two protein bands observed in the RMST pulldown and not in the control ... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas...

Ngày tải lên: 09/09/2015, 17:54