stochastic model of the cell cycle with chemotherapy

... analysis of the complete set of different transcriptional profiles generated in this study involved in most instances the comparison of the profiles of G0-arrested WT cells with those of the other ... participation of the Ras isoforms in cellular responses to the stress of serum deprivation to be gauged On the other hand, the study of the transcriptomic profiles of the same set of serum-arrested fibroblast ... documenting the occurrence of specific transcriptional profiles associated with the absence of H-Ras and/or N-Ras during defined moments of the early stages of the cell cycle Results Analysis of serum-dependent,...

Ngày tải lên: 09/08/2014, 20:20

... If the points have too much scatter, the fine detail of the single cell linear patterns is lost There is also a second RCP at the end of the cycle A much more detailed analysis of populations of ... along with a great deal of skill and hard work In one set of experiments on single cells [17], they made a measurement of the dry mass of single cells by interferometry and then placed it in the cycle ... The selected cells come from more than a very narrow region of the cycle Some of the variation can be reduced by a correction for asynchrony [5] but there is still cell- to -cell variation in cycle...

Ngày tải lên: 13/08/2014, 22:22

... at the beginning of the S phase (0 h), and these marks were attenuated for the rest of the cell cycle (10 and 20 h), correlating with the expression of the gene (Fig 6C,D) The correlation of ... during cell cycle progression, we analyzed the expression profiles of MLL1, CGBP, Ash2 and Rbbp5, together with cyclins E and B, as a function of the cell cycle Western blot analysis of the whole -cell ... affected, they are modulated at the promoters of specific genes over different phases of the cell cycle 1630 Results and Discussion Dynamics of MLL1 and its interacting proteins during the cell cycle...

Ngày tải lên: 23/03/2014, 06:20

... 33 °C) of the intensity of the signals is shown (B) A dominant-negative mutant of human CDK2 was expressed as fusion proteins with HA in HeLa cells The effect of the expressed CDK on the phosphorylation ... revealed by the retarded mobility of the protein in SDS gel, compared with the mobility of protein pre1226 Fig Specificity of binding of phosphoMCM4 antibodies The binding specificity of six antiphosphoMCM4 ... Linearity of DAPI-stained DNA content of a nucleus was preserved throughout such treatments; i.e the DNA content of G2, M cells are almost double that of G1 cells and the DNA content of S cells ranges...

Ngày tải lên: 16/03/2014, 13:20

... until the end of the experiment We then tested whether the plasmodial size (growth rate, Fig 2A,C,E) and the duration of the cell cycle (Fig 2B,D,F) depended on the dose of injected PMLA, over the ... increases the growth rate and shortens the duration of the cell cycle is the polymer-inherent isosterism of the carboxylates with the array of phosphates in nucleic acids and, consequently, the competition ... We suspected that the mechanism(s) underlying the increase in the growth rate and the shortening of the cell cycle might be related to the ability of PMLA to form complexes with nucleic acid...

Ngày tải lên: 16/03/2014, 18:20

... show that the minimum in the inhibitory activity corresponded with a maximum of DNA polymerase activity in the cell cycle The in vivo activity of DNA polymerases The cell cycle dependence of DNA ... mitosis Thus, the in vivo and in vitro activities of DNA synthesis corresponded with the minimum of the inhibitory activity in the cell cycle The activity of DNA polymerases in the nuclear extracts ... whether the intrinsic DNA polymerase activity of the extract varied during the cell cycle or whether it was totally modulated by the degree of complex formation of DNA polymerases with PMLA The...

Ngày tải lên: 31/03/2014, 21:21

... uptake profile, b) [3H]-FAC uptake profile, c) [3H]-FDG uptake profile The black lines and the number next to them represent the average change in PET tracer uptake of the cell lines with the same ... secondary mutations in this group of cell lines, with one cell line having a mutation in EGFR, and one cell line with a mutation in AKT (Table 1) In addition, the M257 cell line, which is wild type ... copies of wild type BRAF and a point mutation in CDKN2A The distribution of amplification events in MITF and EGFR were also spread among the cell lines Of note, there was no clear trend regarding the...

Ngày tải lên: 18/06/2014, 16:20

... mention that the outcome of our experiments was most likely influenced by the stage of the estrus cycle of the dogs from which the oocytes were collected; the positive effect of β-ME on IVM of canine ... important to mention that the outcome of our experiments was most likely influenced by the stage of the estrus cycle of the dogs; the positive effect of EGF supplementation on IVM of canine oocytes was ... supplementation on the base of the stages of the estrus cycle of the ovaries, and demonstrated that β-ME or EGF supplementation significantly increased maturation of canine oocyte to MII stage A number of studies...

Ngày tải lên: 07/08/2014, 18:20

... by the set of oncogene/suppressor genes that are active within the cell and the number of different cellular mechanisms that this set of genes acts upon to change the cell' s behavior with respect ... collect components of cellular behavior that best fit our knowledge of the cellular phenotype; i.e the cell' s growth capacity and survival, the cell' s differentiation status and the cell' s transformation ... in the expression of the oncogene protein but in the alteration in the DNA by the oncogene In the case of Bcr-Abl, it is the observed 9–22 chromosome translocation that affects the behavior of...

Ngày tải lên: 13/08/2014, 23:20

... phases of the cell cycle as well as cell size The simultaneous effect of each dsRNA on six different cellular phenotypes was recorded: the percentage of cells with a DNA content of 2N (percentage of ... size Becuase cells grow as they progress through the cell cycle, dsRNAs that increase the proportion of cells in G2 will also increase the mean cell size of the entire cell sample without necessarily ... delays cell- cycle progression without having an effect on growth or the distribution of cells in the cell cycle These two possibilities could be distinguished by examining the effect of these...

Ngày tải lên: 14/08/2014, 16:21

... stages of the cell cycle 1.4.1 Regulation of DNMT1 expression The DNMT1 protein peaks during DNA replication in the S phase of the cell cycle, but is present at very low levels in non-cycling Go cells ... facilitate cell cycle arrest governed by p53 as well as enhance the apoptotic activity of E2F1, the levels of p53 and E2F1 in cells may have a profound impact on cell cycle progression Other than ... DISCUSSION Regulation of DNMT1 expression in the Cell Cycle 3.1.1 Inverse relationship between DNMT1 and p21WAF1 in the 63 cell cycle 3.1.1.1 3.1.1.2 DNMT1 expression in the cell cycle 63 WAF1 in...

Ngày tải lên: 14/09/2015, 14:13

... percentage of child deaths in the total the losses of life in the flood seasons in the MD Save the Children (2003) reported that the vast majority of the drowning deaths in recent years in the region ... those living in the urban areas, children in the rural area are familiar with the water in rivers and canals Their daily lives are linked with the rice fields and flood plains Some of them learn to ... rowed and fell down the water while sleeping In Vietnam, the first day of the new academic year is usually in early of September In the MD, this period coincides with a high risk of flood Many pupils...

Ngày tải lên: 18/10/2013, 12:15

... problems with the connected devices and the speakers are turned on Gathering information is by far the most important step of the troubleshooting cycle Step Given the above scenario, what are the ... verifying the proper installation of the sound card in the PCI slot If the problem still exists, check for system resource conflicts Finally, update or reinstall the driver Step The first aspect of the ... solution involves gathering correct information, knowledge of the computer components, and the ability to recognize symptoms The problem has been isolated to a bad installation of the sound card -...

Ngày tải lên: 23/10/2013, 01:15

... • the amplitude is the height of the wave the distance from node to crest or node to trough the amplitude is a measure of how intense the light is – the larger the amplitude, the brighter the ... Waves • the frequency, (ν) is the number of waves that pass a point in a given period of time the number of waves = number of cycles units are hertz, (Hz) or cycles/s = s-1 1 Hz = s-1 • the total ... is proportional to the amplitude and frequency of the waves the larger the wave amplitude, the more force it has the more frequently the waves strike, the more total force there is Tro, Chemistry:...

Ngày tải lên: 28/11/2013, 01:11

... working model for the transport cycle of the active TAP variant 2–1C2 The initiation step and the progress of the transport cycle are similar to the hypothesized peptide translocation model of the ... our view initiates the recovery phase of the transport cycle [10] The observed allosteric effect seems to depend on the combination of the core NBD1 with core NBD2, and not of the C-terminal tails, ... [31] As the polypeptide chain of TAP2DV ends with the D-loop region (Fig 1A), the differences in transport activity of the TAP2iso product and TAP2DV might be due to the different length of the C-terminal...

Ngày tải lên: 20/02/2014, 02:21

... range of the changing Fig Calculated uxes in the vicinity of the physiological operating point The steady-state uxes were calculated with the improved version of the computer model in which the ... changes in the concentration of Pi not modify the partition (Fig 3A) However, the presence of Pi considerably affects the dynamics of the system Indeed, in the presence of 10 mM Pi, the model indicates ... improve the model with a simplication of the TS mechanistic equation The improved version of the numerical model was in a very good agreement with the in vitro results and consistent with threonine...

Ngày tải lên: 20/02/2014, 02:21

Ngày tải lên: 07/03/2014, 16:20

... urea cycle intermediates were added to the cells, in order to assess the influence of these added substrates on the rate of urea synthesis Model Written in Mathematica, the basic model simulates the ... Simulating the effect of the addition of mM unlabelled arginine on the distribution of radioactivity in cytoplasmic intermediates of the urea cycle As the pattern of distribution of radioactivity ... [10], but aspects of channelling and the effects of subcompartmentation of the cycle were not explored Hence, the aims of the current work were to: (a) extend this model in the widely available...

Ngày tải lên: 08/03/2014, 08:20

... one the officers stole out from the cabin with bare feet, and made their way up to the quarterdeck, until some thirty of them were gathered there, being all the officers of the regiment, the ... invasion of England He pushed his arguments with fiery zeal, urged the disaffection of all classes, the hatred of the Commons, the defection of the Lords, the alarm of the Church, and the wavering ... what they were, for when they got within a mile of us two black flags ran up to their mastheads "The captain he trained one of the stern chasers hisself, and the first mate took the other They...

Ngày tải lên: 08/03/2014, 15:20

... To execute the second part of the conceptual plan, convert the energy of the pulse from mJ to J Then divide the energy of the pulse by the energy of a photon to obtain the number of photons Epulse ... energy of the electron and the size of the orbital; the angular momentum quantum number (l) determines the shape of the orbital; and the magnetic quantum number (ml) determines the orientation of the ... occur, the spacing of the slits has to be on the order of atomic dimensions 5090X_07_ch7_p252-285.indd 264 The heart of the quantum-mechanical theory that replaced Bohr’s model is the wave nature of...

Ngày tải lên: 09/03/2014, 00:20