stem cells in regeneration and therapy

... embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells by co-culture ... pivotal role in translation initiation by binding eIF4E and eIF4A, by forming a bridge between the mRNA and the ribosome, and by binding PABPC1 to facilitate translation of poly(A)-containing mRNAs ... stored mRNAs RINGO/Spy protein is absent in the oocyte cytoplasm, while its mRNA is maintained in a dormant state This is achieved by binding of Pumilio-2, an RNA-binding protein initially described...

Ngày tải lên: 22/03/2014, 20:21

... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... medium containing 100 U/ml penicillin, 100 μg/ml streptomycin, and mM L-glutamine, and subsequently resuspended in 100 ul (0.5 M cells per 100 ul) of buffer (PBS+0.5% BSA) Cells were stained with...

Ngày tải lên: 18/06/2014, 16:20

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... stained with Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic media and confirmed calcium deposition A and B: Oil red O stain, C and D: Alizarin red S stain, ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells...

Ngày tải lên: 07/08/2014, 23:22

... of injection, the hearts were harvested, washed in PBS, and fixed in 10% formalin overnight at 4°C Paraffin embedded tissues were cut into 5-μm sections and stained by Masson’s Trichrome staining ... in prolactin-mediated cell proliferation and angiogenesis in human endothelial cells Int J Mol Me 2002, 10:433-440 Zeng B, Ren X, Lin G, Zhu C, Chen H, Yin J, Jiang H, Yang B, Ding D: Paracrine ... intraperitoneal injection of the HO-1 inhibitor zinc-protoporphyrin (ZnPP, Porphyrin Products, Logan, UT, USA) at a concentration of 50 μmol/kg/day, starting two days before and continuing until days...

Ngày tải lên: 10/08/2014, 05:21

... such as intestine [52] and from the ectodermal tissues including skin [53], deciduous teeth [54] and nerve tissue [8,9,55,56] ASCs originate during ontogenesis and remain in a marginal area in a ... islets and reintroduce physiological secretion of human insulin AHSCT improves b -cells function and frequently decreases the exogenous insulin need [20] or induces a persistent insulin independence ... enlargement and blood clots in veins and arteries Surgery is the usual therapy and, in many cases, is followed by chemotherapy [234-236] The gastrointestinal tract is a target of GVHD in transplants and, ...

Ngày tải lên: 10/08/2014, 10:20

... were fixed in formalin (4%) until further procession Formaline-fixed microspheres were dehydrated in increasing ethanol concentrations (50%, 75%, 90% and 100%) and embedded in paraffin (Paraplast ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... slides, deparaffinized with xylol and rehydrated in decreasing ethanol concentrations Samples were stained with alizarine red solution (2%) to detect calcium and counterstained with toluidine blue,...

Ngày tải lên: 11/08/2014, 20:21

... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and...

Ngày tải lên: 13/08/2014, 23:20

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving...

Ngày tải lên: 11/09/2015, 09:16

... expand and differentiate [6] Their origin could be in embryonic cells or in progenitors unrelated to embryonic stem cells Adult stem cells are undifferentiated cells occurring in tissues and ... capacity [42] Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a ... minimizing the risk of rejection ASCs regulate T cell function by promoting induction of suppressor T cells and inhibiting production of cytotoxic T cells, NK cells, and proinflammatory cytokines...

Ngày tải lên: 18/06/2014, 16:20

... and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing ... mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups with seven mice in each ... DNR-mediated apoptosis in HepG2r/ADM cells, we investigated apoptosis-related protein expression in the cells (Figure 6C) DNR or Cdte QDs cannot induce apoptosis strongly in HepG2r/ADM cells due to multidrug...

Ngày tải lên: 21/06/2014, 03:20

... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C ... latipsoH gnihcaeT lacideM yranireteV ytisrevinU lanoitaN luoeS ta god lergnom gk 85 a fo noitces nairaseaC eht gnirud deniatbo saw BCU :noitcelloc lleC sCSM-BCU eninac fo snoitaraperP dehsilbatse saw ... yliad semit eerht tsael ta demrofrep saw noisserpxe reddalb launam ,dedeen fi dna ,tinu erac evisnetni na ni derotinom erew sgod eht ,noitarepo eht retfA h 21 retfa devomer neht dna ,erutus epyt...

Ngày tải lên: 07/08/2014, 23:22

... centrifugation Cells were resuspended in buffer containing 0.1% bovine serum albumin and 0.1% NaN3 at 106 cells/ ml Nonspecific antibody binding at the Fc receptor was blocked by treating 106 cells with ... antigen-experienced T cells, are mainly CD28– and can also express CCR5 [10,23,24] The fraction of CD28– T cells is also expanded in WG and is correlated with organ involvement [25–28] These findings support ... CCR5+ and CCR3+ T cells owing to enhanced recruitment into inflammatory sites Downregulation of the cell-surface expression of the inducible inflammatory chemokine receptors CCR5 and CCR3 during...

Ngày tải lên: 09/08/2014, 01:21

... delivered via either adenoviral infection or osmotic pumps in inhibiting bleomycin-induced increases in TNF-α, IL-1α, and trafficking of lymphocytes and neutrophils into the lung [56] These successful ... and IL-6, and secrete MMPs, leading to the systemic inflammation that eventually results in joint destruction [78,80] Pharmacological interventions aiming at reducing inflammation, including methotrexate ... Technology insight: adult stem cells in cartilage regeneration and tissue engineering Nat Clin Pract Rheumatol 2006, 2:373-382 Bianco P, Robey PG, Simmons PJ: Mesenchymal stem cells: revisiting history,...

Ngày tải lên: 09/08/2014, 13:22

... for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as ... were stained with only haematoxylin Three pathologists (M.N., H.O., and T.N.) independently made a diagnosis using the μm sections stained with haematoxylin and 2 Immunohistochemical Staining The ... between LCNEC and LCC, and 453 in the overlap among three groups; SCLC contained a total of 1,096 including 362 unique, 100 in the overlap only between SCLC and LCC and the overlapped proteins described...

Ngày tải lên: 10/08/2014, 09:22

... of stem cells and for understanding their behaviour in physiological and pathological conditions Niches are local tissue microenvironments that maintain and regulate stem cells [52], Livraghi and ... systems Labeled cells retain both biological and proliferating activity, and are ideal for in vitro cell labeling, in vitro proliferation studies and long term, in vivo cell tracking In the current ... cell therapy and tissue regeneration a clinical reality[7] In this rapidly expanding field of cell based therapy, more attention has been paid to the relationship between stem cells and tumor cells...

Ngày tải lên: 10/08/2014, 10:21

... YY (a marker of early endocrine cells) , and endocrine hormones including insulin, glucagon, and somatostatin (39) Although lineage tracing has yet to be performed on cells grown under these experimental ... of proinsulin to insulin In addition, many of the insulinpositive cells were positive in the TUNEL assay, suggesting that they were undergoing apoptosis Finally, it was demonstrated that cells ... Lumelsky and coworkers (65) generated a population of nestin-expressing cells from mouse ES Chapter / Embryonic Stem Cells 11 cells through growth of EBs in serum-free media containing insulin, transferrin,...

Ngày tải lên: 10/08/2014, 18:21

... of stem cells derived from From: Contemporary Endocrinology: Stem Cells in Endocrinology Edited by: L B Lester © Humana Press Inc., Totowa, NJ 49 50 Mitchell Fig Stem cells and origins from inner ... Chapter / Germ Line Stem Cells 45 46 Nagano MC Homing efficiency and proliferation kinetics of male germ line stem cells following transplantation in mice Biol Reprod 2003;69:701–707 47 Shinohara T, ... stem cells, including many that are expressed in ES, EG, and neural precursor or stem cells (Table 1) In addition, umbilical cord matrix cells can be easily expanded and maintained in culture for...

Ngày tải lên: 10/08/2014, 18:21

... ng/mL bovine insulin) as rat insulin standards in the RIA kit No insulin was detected in any of the negative controls analyzed Pancreatic β cells induced from adult pluripotent stem cells as ... Expression of insulin, glucagon, and somatostatin in adult rat pluripotent epiblastic-like stem cells, pluripotent epiblastic-like stem cells induced to form germ layer lineage endodermal stem cells, ... to insulin, glucagon, and somatostatin Visualization of bound antibody occurred with 3,3′-diaminobenzidine (DAB) Original magnifications, ×100 (A) Minimal intracellular staining for insulin (B)...

Ngày tải lên: 10/08/2014, 18:21

... In this circumstance, insulin-producing cells were present, but cells exhibiting characteristics of exocrine cells, including expression of trypsin, were also present (44,45) Interestingly, insulin ... differentiation of nestin-positive cells, approximately 80% of Pax4-expressing cells produced insulin Growth of cells in histotypic culture resulted in spheroids containing cells with insulin-positive granules, ... ES cells into insulin-secreting cells have been developed (23–25) To date, the efficiency of generating insulin-secreting cells using these protocols has been low, and the cells have, in general,...

Ngày tải lên: 10/08/2014, 18:21

... cellulin in liver cells induces insulin expression in rodent and human cells (101–104) This particular finding should be of much interest because certain progenitor cell populations in the fetal ... generating hepatocyte-like cells by manipulating cultured ES cells both in vitro and in vivo (40–44) Embryoid bodies derived from ES cells showed albumin and α-fetoprotein expression and capacity to synthesize ... islet-derived progenitor cells into insulinproducing cells Endocrinology 2002;143:3152–3161 54 Delacour A, Nepote V, Trumpp A, Herrera PL Nestin expression in pancreatic exocrine cell lineages Mech Dev...

Ngày tải lên: 10/08/2014, 18:21