starch its isolation separation and identification

... 16S-9F and 16S-1525R The result of PCR perform size band of strains and Bacillus subtilis control had 1500bp Sequencing perform that strains (Bs4 and BN5) were Bacillus subtilis (99% and 98% ... environment and organic matter should be decided on the subject of Bacillus subtilis isolated from waste water of beer and beer wallows in the brewing process Objective: Isolation, selection and identification ... and storaged in refrigerator 2.2.2 Observation shape and movement of bacteria After isolation and separation bacteria, observation the movement and shape in sterile distilled water Preparation of...

Ngày tải lên: 06/10/2015, 12:58

... P010726-22, P010726-23, P010726-25, and O157-C-1-2), and 14 USA isolates; strains of ATCC (A1, A2, A3, and A4), strains of Cornell University (C1, C2, C3, C4, C5, and C6), and strains of Pennsylvania ... Patararungrong N, Pungrasamee P, Jitsurong S, Morigaki T, Asai N, Nishibuchi M Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand FEMS Microbiol Lett ... eaeA, and hlyA in multiplex PCR assay, (iii) to compare the genetic patterns of Korean isolates and U.S isolates, and (iv) to compare the efficiency among conventional culture method, IMS, and...

Ngày tải lên: 07/08/2014, 18:21

... giant pandas’ spleens, infected and non-infected FCWF cells (as positive and negative controls) using the TRizol Reagents kit (Invitrogen, USA) per the manufacturer’s recommendations o and the ... Bacteriology failed to grow organisms in cultures inoculated with liver and spleen after 48 h culture Fig Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line (A) ... bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus strain from giant pandas 263 isolated The electron microscopy results provided...

Ngày tải lên: 07/08/2014, 23:22

... (H9N2 and H5N1) and human influenza A H1N in vitro than andrographolide [83] Another andrographolide analogue 14-glycinyl andrographolide hydrochloride inhibits virulence factor production and ... microglial activation and production of proinflammatory factors such as TNFα, NO and PGE2 [70] Andrographolide inhibits OVA-induced increases in total cells, eosinophils and IL-4, IL-5 and IL-13 levels ... chitinases, mucin Muc5ac and iNOS in lung tissues and airway hyperresponsiveness [71] Andrographolide inhibits OVA-induced increases TNF-α and GM-CSF in BALF of OVA-sensitized and nasally-challenged...

Ngày tải lên: 13/08/2014, 15:21

... Discussion and conclusions Chapter Isolation and characterization of components from ethyl acetate fraction of Ixeris sonchifolia Hance 48 55 3.1 Introduction 56 3.2 Materials and methods 56 3.2.1 Isolation ... Molecular and Cell Biology, who helped me process the tissue immunohistological staining I would like to thank Dr Chan Lai Wah and Dr Go Mei Lin for their valuable guidelines on my Ph.D candidature and ... continuous encouragement, wise advice and kind support Her support, understanding, advice and mentoring have helped guide me through my doctoral program and my dissertation Without her, none...

Ngày tải lên: 11/09/2015, 10:06

... and Pitas, 1997; Pigeon and Vandendrope 1997; Yang and Huang, 1994], feature invariant approaches [Kjeldsen and Kender, 1996; Leung, Burl and Perona 1995; McKenna, Gong and Raja, 1998; Yang and ... “divide and conquer” strategy is a means to solve this “curse of dimensionality” Face pattern detection [Li, Zhu, Zhang, Blake, Zhang and Shum, 2002; Pentland, 2000a; Pentland 2000b; Pentland and ... Yow and Cipolla, 1997] and template matching approaches [Venkatraman and Govindaraju, 1995; Sinha, 1995; Lanitis, Taylor and Cootes, 1995; Govindaraju, Srihari and Sher, 1990; Craw, Tock and...

Ngày tải lên: 12/09/2015, 10:37

... Xu B and Song J Prevalence and risk factors of overactive bladder syndrome in Fuzhou Chinese women Neurourol Urodyn 2006; 25:717 Teleman PM, Lidfeldt J, Nerbrand C, Samsioe G, Mattiasson A and ... there was a statistically significant association between OAB and hepatitis (p=0.03, OR=2.2) See Table Table Prevalence of OAB, LUTS and OAB subtypes DISCUSSION The prevalence of OAB in men has ... urinary symptoms (OAB, LUTS and/ or BPH) Furthermore, those with OAB had a worse quality of life score Mean quality of life score for those with OAB was 3.4 of 6, and those without OAB 1.6 Thus,...

Ngày tải lên: 25/10/2012, 11:35

... distress and sexual dysfunction in patients with lymphedema were low levels of social support [10] It was surmised that social support may help combat fears of abandonment and feelings of isolation ... their conditions and using generic metric tools for the assessment of physical and mental deficits represent both approaches that in conjunction can effectively follow-up rare, and commonly related ... that may suffer from the same problem”, and when information on his professional status was asked he added: “I could not spend too much time standing up and I lost my job” Case presentation The...

Ngày tải lên: 25/10/2012, 11:40

... of the standard The symbols and designations in this standard can depict both hardware and function Sketches and technical papers will usually contain highly simplified symbolism and identification ... e-mail: standards@isa.org The ISA Standards and Practices Department is aware of the growing need for attention to the metric system of units in general, and the International System of Units (SI) ... instrumentation standards The Department is further aware of the benefits to U.S.A users of ISA standards of incorporating suitable references to the SI (and the metric system) in their business and professional...

Ngày tải lên: 04/04/2013, 12:40

... is bound up its love of liberty, its independence, its passionate resistance to foreign conquerors, its devotion to lost causes, its loyalty to the throne, its pride, its trade, its maritime ... presently to the cabin and stand behind me, and knock down but one man, in case two laid on me, and I would kill and command all the rest presently.' The boy, however, was timid, and when Lyde, to ... 'paying their dues and doing their suits and services.' The 'suits and services' involved attendance at the Prince's Courts, and the tenants' help at the time of the bullock and pony drifts that...

Ngày tải lên: 21/02/2014, 21:20

... Physics 24 (2008) 209-222 identification, dynamic response and so on Several literatures presented the POD’s application to decompose the spatially-correlated and multi-variate random pressure fields ... of the random pressure fields almost have based on the covariance matrixbranched POD due to its straightforward in computation and interpretation Some authors used the POD to analyze random pressure ... troublesome and difficulties in interpreting theses results In this paper, the POD based spectral and covariance matrices of the random field will be presented Both covariance-based and spectral-based...

Ngày tải lên: 05/03/2014, 14:20

... primers annealing at the beginning and end of the cDNA and sequenced Sequence analysis and homology modeling DNA and amino acid sequences were processed, analyzed and aligned with the vector nti ... at 50 °C, and 60 s at 72 °C, and 10 at 72 °C The products of the PCR reaction were separated with HE 33 Mini agarose electrophoresis apparatus (Hoefer, Holliston, MA, USA), and the band of interest ... for the structural and functional properties of the enzyme, but might have an important role in determining its substrate specificity and in the uncoupling between substrate and Ca2+ binding [31]...

Ngày tải lên: 06/03/2014, 11:20

... rat HDC monomer and its quaternary structure, obtained after docking and energy minimization calculations carried out as described in the Materials and methods section One thousand dimer structures ... aminotransferase lowers its pKa in the unliganded enzyme and is crucial for the successive increase in the pKa during catalysis Biochemistry 37, 15076–15085 Guex, N & Peitsch, M.C (1997) SWISS-MODEL and the ... to adopt a-helix and random coil secondary structures (predictions not shown, also derived from Figs and 2) Concluding remarks Since the initial suggestions by Pauling in 1948 and the later formulation...

Ngày tải lên: 08/03/2014, 08:20

... national banks State and national banks may accept several different categories of deposits, including demand deposits and other transaction accounts, time deposits, and savings deposits These different ... (NOW), and share draft accounts nationwide; phased out interest ceilings on time and savings deposits; and broadened the investment and lending powers of savings and loan associations and savings ... demand deposits Banks are also required to maintain reserves, as specified by the Federal Reserve System, against their demand deposits and other transaction accounts Time deposits are deposits...

Ngày tải lên: 15/03/2014, 10:20

... an endogenous ligand which binds to site II, and of cefazolin (site I-ligand) and verapamil (site I and II-ligand) as exogenous ligands All these ligands are known for their high binding efficiencies ... between reduced and oxidized HSA, we examined two functional properties, ligand-binding properties and antioxidant activities, using both purified nonoxidized and oxidized HSA samples Ligand-binding ... functions of HSA [26] HSA binds and transports numerous endogenous and exogenous compounds, and controls their solubility and toxicity in vivo Among the several endogenous ligands of albumin, we focused...

Ngày tải lên: 16/03/2014, 13:20

... of protein (lanes and 4, VHD-caveolae; lanes and 5, HD-caveolae; lanes and 6, LD-caveolae) The amount of the analysed proteins is expressed as arbitrary densitometric units and normalized to ... centrifugation, and analysed by SDS ⁄ PAGE and immunoblotting for the distribution of caveolin (——), perilipin ( ), and SR-BI (– — –) The HDcaveolae were collected at the 26 ⁄ 35% sucrose interphase and ... before isolation of the caveolin-containing membranes After SDS ⁄ PAGE, blotting, and detection with horseradish peroxidase-labelled avidin, biotinylated A Fig Separation of HD-caveolae and LD-caveolae...

Ngày tải lên: 16/03/2014, 13:20

... [7.7]/[11.3] µm and [8.6]/[11.3] µm ratios, but the 6.2, 7.7, and 8.6 µm features include blended PAH+ and PAH0 bands As we show here, the 11.0 µm band is a purely cationic band and the 11.2 µm band is ... splitting of the initial 11.2 µm PAH band into two bands at 11.0 µm and 11.4 µm due to the Si adsorption on the PAH edge creates and a blue-shifted 6.2 µm band We argue here (see Section 6.2), ... band stretches from 12.5 to 13.0 µm Comparing the PAH+ and PAH0 spectra, there is a systematic 0.2 µm blue shift between the emitting bands We not observe this shift in the 13.5 µm band PAH band...

Ngày tải lên: 16/03/2014, 16:20

... GLUT1 and GLUT3 (exons 3–8), and four in human GLUT2 (exons 7–10) and GLUT4 (exons 6–9) (Table 1) The codons for arginine (96) and valine (231) in gcGLUT (Fig 2) are split between exons and 5, and ... (between exon and exon 5) and valine (between exons and exon 7) are shown Exonic regions are shown in uppercase and intronic regions are in lowercase The split codons are boxed and highlighted ... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and...

Ngày tải lên: 17/03/2014, 03:20