standardization of diagnostic assays for animal acute phase proteins

... 1.1.1 of this Aquatic Manual) The establishment of quality Manual of Diagnostic Tests for Aquatic Animals 2009 13 Chapter 1.1.2 — Principles and methods of validation of diagnostic assays for infectious ... diagnostic tests because they not require validation for diagnostic Manual of Diagnostic Tests for Aquatic Animals 2009 17 Chapter 1.1.2 — Principles and methods of validation of diagnostic assays ... error rates of diagnostic tests Biometrics, 36, 167–171 22 Manual of Diagnostic Tests for Aquatic Animals 2009 Chapter 1.1.2 — Principles and methods of validation of diagnostic assays for infectious...

Ngày tải lên: 21/08/2013, 13:57

... Critical evaluation of diagnostic aids for the , Oral Oncol (2007), doi:10.1016/j.oraloncology.2007.06.011 ARTICLE IN PRESS Critical evaluation of diagnostic aids for the detection of oral cancer ... Critical evaluation of diagnostic aids for the , Oral Oncol (2007), doi:10.1016/j.oraloncology.2007.06.011 ARTICLE IN PRESS Critical evaluation of diagnostic aids for the detection of oral cancer ... Critical evaluation of diagnostic aids for the , Oral Oncol (2007), doi:10.1016/j.oraloncology.2007.06.011 ARTICLE IN PRESS Critical evaluation of diagnostic aids for the detection of oral cancer...

Ngày tải lên: 06/03/2014, 02:21

... to the nature of the diagnostic specificity of the antibodies used and the properties of the target antigen Much of this information is theoretical and beyond the control of the diagnostic laboratory ... extensive assembly of panels of antibodies for application to a wide variety of diagnostic situations, providing an easy-to-use practical guide The addresses of worldwide suppliers of these reagents ... primary panel of antibodies provides the major categorization of the tumor and a secondary panel allows further subtyping For example, positivity for CD30 will be useful for the confirmation of the...

Ngày tải lên: 11/04/2014, 07:12

... Number of Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes...

Ngày tải lên: 28/04/2014, 13:08

... transcriptional activation of APP genes (Rousseau et al., 2008) Indeed, GATA4 represses 16 Acute Phase Proteins – Regulation and Functions of Acute Phase Proteins C/EBP isoform activation of the KNG1 and ... responses of circulating leukocytes, and by forestalling endothelial activation 26 Acute Phase Proteins – Regulation and Functions of Acute Phase Proteins A particularly important role for APPs ... receptor-gamma co-activator-1alpha in the acute- phase response Biochem J., 415(2): 289-296 24 Acute Phase Proteins – Regulation and Functions of Acute Phase Proteins Wang, Z., & Burke, P A (2010)...

Ngày tải lên: 27/06/2014, 19:20

... concentration of 100-150 ng/µl, and the template for this concentration was used in a normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, ... each of the PCR tubes One or µl of DNA was added PCR was performed in the PCR machine (Gene Amp PCR system 9700) with an initial 95oC step for minutes and 30 cycles of denaturation at 94oC for...

Ngày tải lên: 06/08/2014, 19:20

... the expression of the overexpressed high abundance acute- phase proteins (APPs) in sera of the EOCa patients was analysed according to the stages of the cancer Image analysis Images of the 2-DE gels ... protein profiling for diagnosis of pancreatic cancer reveals the presence of host response proteins Clin Cancer Res 2005, 11:1110-1118 Yu KH, Rustgi AK, Blair IA: Characterization of proteins ... analysis of 2-DE gels When the clusters of the high abundance serum protein spots from control women and cohorts of patients diag- Page of (page number not for citation purposes) Journal of Hematology...

Ngày tải lên: 10/08/2014, 22:20

... protein (LBP) [3,4] The acute phase proteins used in diagnostic procedures are produced mainly by hepatocytes Two classes of acute phase proteins may be distinguished on the basis of their synthesis ... marker of the acute phase response • 50 LBP is not a suitable parameter for differentiating between infectious and noninfectious etiologies of SIRS 20 10 1st examination Last examination Median of ... isolation of an organism of recognized pathogenic potential from an intravascular catheter removed for infection related reasons All patients were screened on a daily basis for the presence of pathogenic...

Ngày tải lên: 12/08/2014, 20:20

... models The acute phase response consists of changes in the serum concentration of multiple proteins due to a reorganization of hepatic protein synthesis favouring "the acute phase proteins" and ... decrease of certain proteins during the acute phase response is presumably caused by the need to divert available amino acids into the production of active acute phase proteins Albumin is one of these ... raise a reduced acute phase response induced by LPS Therefore, we measured the plasma concentrations of selected important acute phase proteins and the expression in liver tissue of their genes...

Ngày tải lên: 13/08/2014, 13:20

... NANOPARTICLE FORMULATIONS OF DIAGNOSTIC AGENTS FOR MEDICAL IMAGING WANG YAN (B Eng, Shanghai Jiao Tong University) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE PROGRAMME ... for qualitative determination of diagnostic agents encapsulated, inductively coupled plasma-mass spectrometry and micro-plate reader measurement for quantitative determination of the amount of ... concentrations of 0.5, 5, 50 or 250µg/ml for 24h incubation The nanoparticles were sterilized by γ-irradiation for 72h before the test wells were used for each sample One row of 12 wells was...

Ngày tải lên: 26/11/2015, 22:40

... National Board for Laboratory Acute phase proteins in calves Animals, Uppsala, Sweden approved the experimental studies (I-III) performed The average age of the animals at the start of the experiments ... inoculation with a daily dose of 250 larvae for days, while a dose of 100 larvae daily for days (experiment II) gave the smallest reactions A sin- Acute phase proteins in calves gle high dose, or a ... clinical signs such as coughing and dyspnoea of varying degrees The prepatent phase is 24 days, and after a patent phase of approximately 60 days the recovery phase follows and clinical signs are vanishing...

Ngày tải lên: 12/08/2014, 15:20

... Western blot analysis of cell extracts with antibody to GFP showed that the abundance of all three fusion proteins diminished in the presence of CHX (Figure 4a) The half-life of the proteins determined ... Two of the proteins showed a half-life of about hour, while the proto-oncogene protein appears to initiate abrupt degradation within about hours of treatment with CHX The results for all three proteins ... expressing EGFP or d1EGFP, a destabilized form of GFP, and analyzed as in (a) native non-fusion endogenous counterparts of two of the proteins we identified, those for which antibodies were available...

Ngày tải lên: 14/08/2014, 14:21

... therefore be a surrogate of disease activity, rather than an independent measure of irreversible loss of function [30,31] Our introduction of the CDAI was not intended to suggest that the acute phase ... indicative of high clinical conformity of classifications by different scores All of these findings indicate that content validity of the CDAI is well maintained despite the absence of CRP as ... limit theorem Analysis of the contributions of acute phase reactants to current composite scores Calculations of the DAS28 and SDAI are based on the following: numbers of swollen and tender joints...

Ngày tải lên: 09/08/2014, 06:22

... uptake of change [9] Theoretical models underpinning change are useful for guiding the design of diagnostic analysis For example, elements of diagnosis are evident in some stage models of change, ... utilising formal and informal interviews, focus groups, observation of meetings and clinical practice to identify organisational barriers for change Wensing and Grol [23] emphasised the value of diagnostic ... usefulness of a specific combination of methods for undertaking diagnostic analysis Methods Setting The study was conducted in an acute NHS Trust (600 beds) over a six-month period and formed stage...

Ngày tải lên: 11/08/2014, 05:22

... Figure 2: Formalization of the morphological layer and excerpt of a sample RDF instantiation to the original meaning of the word by reduplication, ... change of the original word Change of parts -of- speech by affixes Affixes change parts -of- speech of words in Thai (Charoenporn et al., 1997) There are three prefixes changing the part -of- speech of the ... singular ...

Ngày tải lên: 20/02/2014, 12:20

... Development of a genetic indicator of biodiversity for farm animals 119 Software tools for management of genetic variation 120 Demonstration of the opportunities offered by a software for inbreeding ... Secretary Prof Kostas Fegeros Rector of the Agricultural University of Athens and President of the Hellenic Society of Animal Production General Director of Animal Production, Dept Ministry of Rural ... Dept of Animal Sciences and Aquaculture of the Agricultural University of Athens Members Prof Stelios Deligeorgis Dept of Animal Sciences and Aquaculture, Agricultural University of Athens Prof...

Ngày tải lên: 22/02/2014, 05:20

... investigation of burst phase kinetics This is the rationale for using calorimetry in the current work Experimental analysis of the burst phase often utilizes the intersection p of the ordinate ... strand The notion of two partially overlapping phases of the slowdown is interesting in the light of the experimental observations of a double exponential decay reported for the rate of cellulolysis ... compilation ê 2011 FEBS Burst phase of processive cellulases C(t) (àMãs1) E Praestgaard et al Fig Rate of cellobiose production CÂ(t) as a function of time for S0 = 70 lM One aliquot of 50 nM Cel7A was...

Ngày tải lên: 28/03/2014, 23:20

... weight loss or deteriorating level of function Sputum smear x Sputum smear x FNAB of LN ≥ cm for TB microscopy (+/- culture and cytology) FNAB of LN _>2cm for TB microscopy (+/- culture and cytology) ... working in In order to increase specificity for the diagnosis of active TB and not overload the TB service, the following criteria had to be met before referral for empirical TB treatment: (i) cough ... the outskirts of Cape Town with a population of approximately 400 000 people and a TB incidence rate of 612/100 000 in 2005 (Virginia Azevedo, Khayelitsha District Manager, City of Cape Town –...

Ngày tải lên: 29/03/2014, 03:20

... Institutional Review Board (IRB) of the Institute of Medical Science at the University of Tokyo and written informed consent was obtained from all of the patients at the time of enrollment The A24-LCL, ... (Department of Surgery and Bioengineering, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo) We also thank Dr Paul D Robbins (Professor of Department of Molecular ... NKI-beteb, HMB-50 and HMB-45, which are used as diagnostic markers for human melanoma [15] The reactivity of HMB-45 on formalin-fixed-embedded specimens of malignant melanomas was shown to be approximately...

Ngày tải lên: 18/06/2014, 16:20