stable transfection pools for large quantity of protein production

... COFRADIC and protein modifications K Gevaert et al large number of species, and it now suffices to generate partial protein sequence information with which to access entire (predicted) protein ... et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... availability of massive numbers of DNA sequences, recent developments in MS, and bioinformatics tools that link DNA and protein sequences to information generated by different types of mass spectrometers...

Ngày tải lên: 18/02/2014, 16:20

... approaches offer generic advantages for the analysis of protein complexes both in terms of dynamic range (i.e how little of one protein can be identified in the presence of how much of a mixture of other ... Analysis of protein complexes Within the scope of this review, we will focus on summarizing the current technical state of the art for the biochemical retrieval of associated proteins and protein ... 577 large excess of contaminating proteins (e.g antibodies in IP experiments) Conclusions The combination of affinity methods for the purification of protein complexes and the identification of their...

Ngày tải lên: 31/03/2014, 07:20

... so we used a window size of ten for the remainder of our analysis There are no qualitative differences in the nature of groups identified by larger window sizes Many of the ten-gene groups that ... pattern of gene expression that mirrors this Journal of Biology 2002, 1:5 http://jbiol.com/content/1/1/5 Journal of Biology 2002, Table The number of groups of genes, and total numbers of genes ... a significant decrease in the numbers of Table The number of groups of genes, and total numbers of genes in groups, that are identified at various levels of significance (p values) Groups Significance...

Ngày tải lên: 06/08/2014, 18:20

... uniquely solvable for every pair of elements a, b in X The order of a quasigroup (X, ◦) is the size of X A quasigroup of order v is called idempotent if the identity x ◦ x = x holds for all x in X ... DTRIQ(v) for any positive integer v ≡ 0, 3, (mod 12) Transitive IQ has been used to give a tripling construction for large sets of STSs in Teirlinck [16] To consider the similar problem for large ... 4643-4652 [8] Q Kang, Large sets of resolvable MTS and DTS of order pn + 2, J Combin Des 4(1996) 301-321 [9] Q Kang, Y Chang, A completion of the spectrum for large sets of disjoint transitive triple...

Ngày tải lên: 08/08/2014, 01:20

... reliability of a hit protein structure For the purposes of scanning a large protein structure database, 3D-BLAST is fast and accurate and is useful for the initial scan for similar protein structures, ... assignments For most sets of sequence identities, 3D-BLAST outperformed PSI-BLAST (Figure 7b) in protein evolutionary classification assignments Almost 70.16% (362 out of 516 proteins) of query proteins ... significance of an alignment in order to determine the reliability of the prediction 3D-BLAST maintains the benefits of the BLAST tool in terms of ordering hit proteins by E value for rapid scanning of...

Ngày tải lên: 14/08/2014, 18:20

... State Bank of Vietnam Agriculture Bank Insurance Joint Stock Corporation Vietnam Bank for Agriculture LIST OF TABLES AND DIAGRAM Table 1.1 Fundamental information of Vietnam Bank for Agriculture ... coordination with Taxation and Customs Offices for the collection of state budget; 2010: Be in Top 10 of 500 largest enterprises in Vietnam and continuously to be the largest Financial Institution in ... also the largest bank in Viet Nam in terms of workforce, operating network and customer base As of October 2013, the operating network of Agribank includes 2,400 branches and transaction offices...

Ngày tải lên: 20/10/2014, 15:22

... of the incompleteness of the network The 'degree' of the network is defined as the average number of links per protein and captures the connectivity of the network Except for Reactome, each of ... outperforms other approaches for identifying human protein names in Medline abstracts, such as the simple matching of words to a dictionary of protein names, as well as the other available protein ... composite human protein interaction map, with network properties of each of the datasets Dataset Version Number of interactions Number of proteins Clustering Connectivity ...

Ngày tải lên: 14/08/2014, 14:21

... initial conformation of a protein crystal as the native conformation deposited in protein data bank (PDB) for a given protein crystal in a unit cell Such initial confirmation for a protein crystal ... role of native topology on the mechanical properties of biological protein materials, we introduce the dimensionless quantity Q representing the degree of folding topology of proteins For a protein ... present the mesoscopic model of biological protein materials composed of protein crystals prescribed by Go potential for characterization of elastic behavior of protein materials Specifically,...

Ngày tải lên: 22/02/2014, 08:20

... eliminate the effects of cAMP by mutating S385, S401 and T407 may be that isoforms other than GIRK1 may be also targets for protein phosphorylation in the protein complex: for example, GIRK2 had ... previously Plasmids for the production of recombinant protein were constructed by a PCR, where forward and reverse primers encoded the desired parts of the C- and N-termini of GIRK1 These sequences ... Cloning, edn CSH, Cold Spring Harbour 36 Bradford MM (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein- dye binding Anal Biochem 72,...

Ngày tải lên: 07/03/2014, 00:20

... designed for the sake of generalization, in order to not overfit the input sample The input of the GRA consists of (i) a sample set of document vectors with the correspond, (ii) a maximum number of ... parameters, which are used for balancing the two factors The extensity of cut is defined as a positive function where is a threshold size of cut is called weight of is called weight of the connection between ... following holds true for any concept-formative cluster and any document : # # q #   ‘6 ) ! # q } … # … £ # ˜ # The whole procedure for finding conceptformative clusters consists of two basic stages:...

Ngày tải lên: 08/03/2014, 04:22

... W & Goris, J (1995) Molecular cloning and developmental regulation of expression of two isoforms of the catalytic subunit of protein phosphatase 2A from Xenopus laevis Biochem Biophys Res Commun ... third subunit of a novel trimeric form of PP2A from rabbit skeletal muscle [28] By cloning the cDNA from a human heart cDNA library, a second cDNA was isolated coding for a protein of 130 kDa with ... templates for DNA sequencing 5¢-RACE technique 5¢-RACE reactions were performed on about 1.5 lg of total RNA of prophase X laevis oocytes with U of AMVreverse transcriptase (5¢/3¢RACE kit, Roche) For...

Ngày tải lên: 08/03/2014, 08:20

... Gla domain of protein C, have recently been solved [25] As predicted [18,28–30], the overall fold of sEPCR is similar to that of the CD1 ⁄ MHC class I family of proteins, consisting of a b-pleated ... the epitope 100 for RCR-2 on sEPCR is on the face of sEPCR opposite to its known binding site for protein C The stability of immobilized RCR-2 as a capture ligand for sEPCR and protein C is illustrated ... a response of 3600 RU (Upper) Binding of protein C to wild-type sEPCR Approximately 400 RU of sEPCR was captured before sequential injections containing decreasing concentrations of protein C...

Ngày tải lên: 16/03/2014, 18:20

... possibilities For example, the rate-constant for the association of MgATP with a protein kinase is typically of the order of 105)106 m)1Æs)1, which, given a typical dissociation constant of 10 lm for MgATP, ... c and d isoforms are not Thus, use of SB 203580, which has been the main 4632 pharmacological tool employed to date, is transparent to two of the p38 MAPK isoforms Although a number of structural ... mapped sites of protein protein interaction, the basis for and extent of the differences in specificity within the p38 MAPK family are still poorly understood Thus, we have no clear picture of how p38...

Ngày tải lên: 16/03/2014, 22:20

... classification, this is an example of the frame adj-obj -for- to-inf, shown in Figure 2, (using AVM notation in place of COMLEX s-expressions) Part of the output of RASP for this sentence is shown in ... unseen in the output of the classifier (17 vs 28) indicate that the new system is capable of detecting a higher number of SCFs The main reason for better performance is the ability of the new system ... classifier output For adjectives only of the 30 gold standard SCFs are unseen, resulting in better recall (57.6% vs 47.2% for nouns) For verbs, subcategorization acquisition performance often correlates...

Ngày tải lên: 17/03/2014, 04:20

... Army Office of Federal Procurement Policy Office of Management and Budget Office of the Secretary of Defense performance-based services acquisition performance-based services contracting performance ... of change, but each of our cases represents an example of a bottom-up effort to take advantage of new opportunities made available by acquisition reform OSD efforts to promote acquisition reform ... DASW01-01-C-0004 Library of Congress Cataloging-in-Publication Data Camm, Frank A., 1949Recent large service acquisitions in the Department of Defense : lessons for the Office of the Secretary of Defense /...

Ngày tải lên: 23/03/2014, 01:20

... unfolded form of the protein that aggregates and precipitates [23] Mutations that abolish metal binding allow the peptide to assume a conformation close to that of the native protein ) a stable conformation ... expression of the nucleotidebinding domains of P-glycoprotein Eur Biophys J 32, 644–654 11 Rigden DJ (2002) Use of covariance analysis for the prediction of structural domain boundaries from multiple protein ... domain of AMPP Different-length domains were tested, and mutations were made to the sequences of these domains It is known that the location of domain boundaries is critical to the formation of stable, ...

Ngày tải lên: 23/03/2014, 07:20

... and 368 s for ours) and 7∼12 times faster for decoding (2.881 ms for M ALLET, 5.028 ms for C RF ++, and 0.418 ms for ours) This result demonstrates that learning and decoding CRFs for large- scale ... (Sparse) All of our model variants were based on Sparse model For the hyper parameter , we empirically selected 0.001 for Method (this preserves 99% of probability density), for Method 2, and for Methods ... performs 5∼50 times faster for training (1,774 s for M ALLET, 18,134 s for C RF ++, Experiment We evaluated our method on six large- scale natural language data sets (Table 1): Penn Treebank3 for...

Ngày tải lên: 23/03/2014, 17:20

... diagram showing localization of the exon-specific oligonucleotide primers used for PCR amplification a + c for amplification of the larger IA transcript; b + c for amplification of the shorter IB transcript ... levels of expression of XlMGP transcripts IA and IB ODC was used as a loading control RNA extracts used for RT-PCRs were made from pools of five randomly picked embryos Results obtained for egg ... additional evidence for differential promoter usage in vivo These findings indicate that transcription of the larger IA form is important for gastrulation, whereas the shorter IB form is likely to...

Ngày tải lên: 30/03/2014, 16:20

... the family of ion peaks, gave a protein with molecular mass of 13,794 ± Da (Figure 1) The lack of recovery or detection of proteins of the size of CP subunits is likely the result of retention ... possible points of control of pararetroviruses http://www.virologyj.com/content/2/1/33 Figure In vitro releasing of the coat protein from the polyprotein P3 In vitro releasing of the coat protein from ... due to the very basic charge of the protein; estimated isoelectric point (pI) of CP is 9.43 Localization of the protease domain By comparing the known sequence of P3 protein in RTBV with aspartate...

Ngày tải lên: 19/06/2014, 08:20

... noted Transfections of plasmid DNA were performed using µg of pUC19, p(E/ Lp)L1R or p(wtp)L1R All infections were harvested at 24 hpi and titered on BSC40 cells Page of (page number not for citation ... Infections were performed by transfection of µg of pL1R each containing a single Cysteine-to-Serine mutation in the coding sequence at time of infection with VV-TetO:L1R at an MOI of 0.1 under non-permissive ... Expression of L1R cysteine-to-serine double mutants Infections were performed by transfection of µg of pL1R containing double Cysteine-to-Serine mutations in the coding sequence at time of infection...

Ngày tải lên: 19/06/2014, 08:20