sphingolipid metabolism before and after the diauxic shift
Báo cáo y học: "The Proteomic Code: a molecular recognition code for proteins" docx
... regarding the biochemical properties (charge and hydropathy) of the 20 amino acids, and the positions of the stop signals Figure emphasizes the importance of the central nucleotide in the codons, and ... is more significant that the FFEs in 1st and 3rd residues are additive and together they represent the entire FFE of the intact mRNA (Figure 21) That the FFE at the 1st and 3rd codon positions ... sequences immediately before and after the exon There are, of course, no known codons in the intronic sequences, therefore we continued the same phase that we applied for the exon, assuming that...
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Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
... (AtPDIL2-2 and AtPDIL2-3) were identified (supplementary Fig S1) Therefore, it is possible that one of the bands is GmPDIM and the other is a homolog However, it cannot be excluded that the second band ... molecular chaperones and other functional proteins must be strengthened in response to the increased de novo synthesis of seedstorage proteins Therefore, we next measured the mRNA and protein levels ... are folded and assembled into trimers in the ER, and then transported and deposited in the protein storage vacuoles [13] Glycinin is synthesized as a 60 kDa precursor polypeptide and is proteolytically...
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Báo cáo hóa học: "Reference values and physiological characterization of a specific isolated pig kidney perfusion model" doc
... reabsorption and the oxygen consumption [31-34] therefore is used here as a further indicator for the performance of the isolated pig kidney Materials and methods Animals and experimental groups After ... blood, they exhibit unique possibilities for pharmacology and toxicology studies and for the improvement of the graft function after transplantation As the donor kidneys are subject to warm and ... assessed directly after surgical resection (prior to eventual cold storage) and before and after reperfusion atinine for the determination of the exogenic creatinineclearance The substrates are...
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Báo cáo hóa học: " Design and Characterization of a 5.2 GHz/2.4 GHz ΣΔ Fractional-N Frequency Synthesizer for Low-Phase Noise Performance" potx
... are the contributors of the in-band noise All in-band noise sources are referred back to the input of the PLL and shown as ϕnoiseI in Figure The noise from the VCO is referred to the output and ... lower than the ΣΔ intercept with the VCO noise and therefore it is the crucial point in this case that sets the loop bandwidth Thus the loop bandwidth should be set at the point where these two ... optimize their circuits and meet their design goals prior to the expensive fabrication The measured integrated phase noise of the WLAN synthesizer was 0.5◦ rms for the lower band and 0.535◦ rms for the...
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Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... One of these changes occurred at framework of the heavy chain (Asp74Asn) and of the light chain Two of the changes (Thr152Ile and Ser197Gly) occurred at frameworks and 3, respectively, and the third ... for the VH domain of clone C1 were IGHV3-30*18, IGHD2-21*01 and IGHJ2*01, respectively For the VL domain, the germ line and the joining segments corresponded to IGVL1-44*01 and IGLJ1*01 The closest ... kinetics to Cn2 toxin The Langmuir (1 : 1) binding model was used (B) The variation between the theoretical and experimental data (residual values) shows the reliability of the fitting The total yield...
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Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx
... showing the four lineages described in genotype G1 of rotaviruses The strains having the four mutations reported by Rahman and his colleagues are indicated by ●; the four mutations plus others at the ... three out of the four mutations by ■, and three mutations plus others at the primer binding site by ᮀ The open branches indicate one or two mutations at the primer binding site The lineages are ... showed only out of the four nucleotide mismatches and the remainder 4.1% showed out of the four nucleotide mismatches plus others mismatches at the 9T1-1 primer binding site The strains grouped...
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Báo cáo y học: "Validation of a specific measure to assess healthrelated quality of life in patients with schizophrenia and bipolar disorder: the ‘Tolerability and quality of life’ (TOOL) questionnaire" doc
... from the patient perspective the most common adverse events of both typical and atypical antipsychotic drugs [4,18] The aim of the present study was to evaluate the linguistic adaptation and psychometric ... the aim of this work is not to obtain utilities but to measure patient HRQoL, in these multidimensional scales we used the unweighted scores In both cases, the higher the scores, the better the ... values between 0.3 to 0.5, and low for rs < 0.3 This statistic was also applied to determine the relationship between the TOOL and the MADRS, YMRS, and UKU scales These analyses were performed...
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Báo cáo y học: "Association of a specific haplotype across the genes MMP1 and MMP3 with radiographic joint destruction in rheumatoid arthritis" ppt
... radiograph was obtained after a median of year, the second after a median of years and the third after a median of 14 years after disease onset The total Ratingen score refers to the last radiograph ... experiments Both wrote the Materials and methods section RR and GH took care of all patients They collected clinical data and all blood samples and radiographs and scored them by means of the Ratingen ... which Materials and methods Patients and controls The study was approved by the Ethics Committee of the Medical Faculty of the MLU Halle-Wittenberg All patients attended the study after giving written...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
... c19orf10-His fusion protein and the reactivity and specificity of the purified IgY were confirmed using GST-c19orf10 and a panel of unrelated His and GST fusion proteins The antibody was then used to establish ... although the majority of the residues were non-polar The remainder of the protein showed a high degree of conservation between the human sequence and all other sequences (Figure 7) The two cysteine ... participated in the design and analysis of the study, drafted the manuscript, performed c19orf10 in silico analysis and the multiple sequence alignment, purified recombinant protein, and carried out the...
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báo cáo khoa học: "Design and characterization of protein-quercetin bioactive nanoparticles" doc
... indicates the loss of the a-helix during aggregation Meanwhile, the broadening of this band and the increase of the band intensity at 1665 cm-1 implies the increase of the random-coil content in the ... [29] The increase in band intensities shown in Tables and indicates that the added Q led to the reorientation of the indole ring through the adjustment in the torsional angle of the side chain The ... constant at 10% The band at 1611 cm-1 (Figures 10 and 11), which is sensitive to the bound ligands, is a marker of the orientation of the indole ring of Trp with respect to the Ca atom of the peptide...
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Design and characterization of functional novel oligopeptides
... attach the first amino acid residue and subsequent stages of the peptide as it is lengthened The carboxyl end of the peptide is attached to the polymeric support The N-terminus needs protection and ... biocomposites The effectiveness of the crystal growth and inhibition processes depends on the structure and chemistry of the interfaces between organic substrate, mineral, and medium The highly ... disrupts the surface and reduces the rate of crystal growth At the same time, aragonite nuclei, which are not affected by the additive, continue to grow unabated in the supersaturated solution and therefore...
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Design and characterization of interposers for high speed fine pitch wafer level packaged device testing
... governed by the number of probes in the array and their proximity to each other Varying the width of the blade shank increases or decreases the surface area where the blade is attached to the probe ... these considerations address the impact of the probe on the device under test (the semiconductor die), multiple contact of the probe needle to the wafer impacts the probe material itself In the ... to the pattern of the bond pads of the circuit to be tested are punched into the template A ceramic or anodized aluminum ring is epoxied to the probes The ring and epoxy hold the probes in their...
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Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
... acetonitrile in H2O) in the first 15 min, to 66.6% B in the next 40 and to 100% eluent B, in the last 10 The flow rate was 2.5 mLÆmin)1 and the absorbance was recorded at 280 nm The fractions containing ... odorants and other tested pheromones (sexual and nonsexual) This pheromone is known to be involved in the regulation of behavioral sequence including feeding of the larvae, capping of the cells and thermoregulation ... TCTTAAACATTAATGCC 3¢ These primers encoded a Kozak consensus sequence as well as BamHI and EcoRI restriction sites The PCR-amplified fragment was cloned into the BamHI and EcoRI sites of pPIC3,5K and the integrity...
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Báo cáo khoa học: Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage potx
... cells and led to a further increase in the pph3D, psy4D and psy2D cells (Fig 3A, cisplatin treatment; Fig 3B, MMS h recovery) After removal of cisplatin and MMS, the cH2AX levels returned to the ... investigated whether there was any interaction between the phosphatase and the histone Employing lysates from the and S cerevisiae cells AY925 PSY4–MYC13 AY925HA3–PPH3, cH2AX was found in the anti-HA ... damage and maintenance of the cell cycle arrest H2AX was found to be hyperphosphorylated in pph3D, psy4D and psy2D strains in both the absence and the presence of ionizing radiation [12], and in the...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
... underneath the inner nuclear membrane and function to maintain the nuclear architecture [3] The majority of vimentin may be bound to the nuclear lamina from the outside the nucleus [18] The presence ... C3H cells and the GFP–ISP36 protein was transiently expressed The cells were immobilized, and the DNA was stained with DAPI and observed by fluorescence microscopy The round structure in the DAPI ... compartments in the interchromatin space by anti-ISP36 staining (Figs and 9); (c) the immunostaining did not expand into the chromosomal compartment or the cytoplasm (Figs and 9); and (d) some ISP36...
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Knowledge transfer in Tehran University of Medical Sciences: an academic example of a developing country pdf
... contributions SN and RM participated in the design, statistical analysis, and manuscript writing JG designed and conducted the study SN, MG, MS, and MA gathered the data KM assisted in interpreting the statistical ... and 0.76 The questionnaire included the following variables: the percentage of time the participants allocated to research activities, the 'reasons for choosing the research topic', and the researchers' ... system experts The other format is for clinical and basic studies, where the researcher chooses which category the proposal most addresses Nevertheless we confirmed the validity of their choice...
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báo cáo khoa học: " Knowledge transfer in Tehran University of Medical Sciences: an academic example of a developing country" pot
... contributions SN and RM participated in the design, statistical analysis, and manuscript writing JG designed and conducted the study SN, MG, MS, and MA gathered the data KM assisted in interpreting the statistical ... and 0.76 The questionnaire included the following variables: the percentage of time the participants allocated to research activities, the 'reasons for choosing the research topic', and the researchers' ... system experts The other format is for clinical and basic studies, where the researcher chooses which category the proposal most addresses Nevertheless we confirmed the validity of their choice...
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Magnetic resonance spectroscopy correlation with histological analysis in gliomas and structure determination of a hypothetical protein
... medicine to detect and diagnose type of brain diseases at the beginning The more efficient and accurate the diagnosis is made, the higher the possibility for the patient to be cured Therefore it is ... on the correlations and proton chemical shifts provided by the COSY spectrum, major metabolites can be identified On the other hand, an HSQC spectrum can provide both H and 13 C chemical shifts ... reduce the line widths therefore facilitate the assignment of backbone and side chain carbon for proteins larger than 25kDa 12 The introduction of 3D and 4D hetero-nuclear NMR experiments and the...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... all the proteins Both the wild-type PfPDO and the mutant C35S were active in the insulin reductase assay [23], whereas the activity of the mutant C146S and the double mutant was similar to the ... reagents] and separated by SDS/PAGE The appearance of a band in the different conditions used (native, reduced, reduced and denatured) on the different proteins (PfPDO and mutants) is evidence of the ... active sites Refolding of the scrambled RNase in the presence of PDI was used as a positive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin from Alicyclobacillus...
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