sourcing isolation and strain dereplication

... giant pandas’ spleens, infected and non-infected FCWF cells (as positive and negative controls) using the TRizol Reagents kit (Invitrogen, USA) per the manufacturer’s recommendations o and the ... Bacteriology failed to grow organisms in cultures inoculated with liver and spleen after 48 h culture Fig Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line (A) ... bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus strain from giant pandas 263 isolated The electron microscopy results provided...

Ngày tải lên: 07/08/2014, 23:22

... Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in the respective human populations ... of the project WZ, SXY, QS, and TLS collected all samples and performed the RT-PCR experiments for HEV RNA WZ and SXY did the phylogenetic analysis All authors read and approved the final manuscript ... genotype HEV strains with complete genome available in GenBank (including both swine and human original isolates) were used as references in the analysis; their GenBank accession numbers and source...

Ngày tải lên: 12/08/2014, 04:21

... radial and circumferential strain and strain rate by 2DST in normo-, hypo-, akinetic and dyskinetic segments of post-infarct patients 8-13, 30-37, which were not reliably accessible by TDI Radial strain ... Bijnens B Strain and strain rate imaging: a new clinical approach to quantifying regional myocardial function J Am Soc Echocardiogr 2004; 17(7): 788-802 Voigt JU, Flachskampf FA Strain and strain ... velocities (S´ and E´), but not of strain and strain rate values In contrast to our results, one previous study showed significant differences of the peak systolic longitudinal strain between...

Ngày tải lên: 25/10/2012, 11:15

... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...

Ngày tải lên: 05/09/2013, 09:08

... wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf produced a predominant band with an apparent ... purified proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared...

Ngày tải lên: 18/02/2014, 08:20

... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS Half maximal inhibitory concentration (IC50) and median lethal dose (LD50) values from HeLa cells and mice divided ... numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant...

Ngày tải lên: 18/02/2014, 16:20

... these cells Mesenteric BECs and LECs (Fig 5A,B) were also obtained by the same strategy as illustrated in Figs and 4, and were treated with VEGF-A or VEGF-C (Fig 5C) MAPK and Akt phosphorylation ... induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolation and characterization of BECs and LECs We next ... introduced by lipofection and maintained for at least days after transfection as assessed by GFP expression (Fig 4E) These Fig Isolation and characterization of uterine BECs and LECs expressing tsA58T...

Ngày tải lên: 18/02/2014, 17:20

... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and averaged ... Materials and methods Bacterial strain The A radioresistens S13 strain used in this work was isolated as previously described [16,17] This bacterium bears several natural plasmids and is able...

Ngày tải lên: 21/02/2014, 00:20

... column, equilibrated and eluted in 0.1 M Tris/HCl, pH 7.6 and several common protein–protein dissociation salts (1 M sodium chloride, M urea and M guanidinium chloride) and ionic detergents (CHAPS, ... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for...

Ngày tải lên: 21/02/2014, 00:20

... or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents systems, the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ... (2H, m, Hb-2 and Hb-6 ) and 2.00 (2H, m, Hb-3 and Hb-5 ) The 13C NMR spectrum of compound displayed signals at d: 22.8 (C-3 and C-5), 27.5 (C-7), 28.8 (C-9 and C-10), 31.5 (C-2 and C-6), 32.9 ... not be separated from each other by CCC Since K2 and K4 were close to K3 and K5, respectively, curcumol (2) and germacrone (3), and curzerene (4) and 1,8-cineole (5) were hardly separated from...

Ngày tải lên: 07/03/2014, 21:20

... described in the Materials and methods phenolic compounds and alginates, etc and hence preserved the enzymatic activity of lamjapin But the efficiency of extraction was still low and the proteins could ... Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate J Biochem Biophys Methods 11, 13–20 Neuhoff, V., Stamm R & Eibl, H (1985) Clear background and highly ... proteins and also to the low efficiency of extraction Physical properties of lamjapin Lamjapin obtained from the Mono Q column migrated as a single band characterized by both 12% SDS/PAGE and isoelectric...

Ngày tải lên: 08/03/2014, 10:20

... 95% (w/v) ammonium sulfate and incubated for 20 at °C The suspension was centrifuged at 15 000 g for 30 and the pellet was resuspended in 10 mL 30 mM Tris/HCl, pH 7.9, and dialyzed against L 30 ... NcoI and BamHI, and cloned into the pET-8c vector Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas was a gift from  Á M Goldschmidt-Clermont (Universite de Geneve, Switzerland) ... mM Na2EDTA, 7% SDS, and 1% BSA After puri®cation of positive clones, k DNA was extracted and puri®ed as described in [13] cDNA inserts were excised by a digestion with EcoRI and cloned into the...

Ngày tải lên: 08/03/2014, 16:20

... c-terpinene and is therefore designated ClcTS (Fig 3B), C62 and M34 both produced limonene and are designated Cl(+)LIMS1 and Cl(+)LIMS2, respectively (Fig 3C,E) and D85 produced b-pinene and is designated ... C62 (D) D85 and (E) M34 B93 mainly produces c-terpinene, C62 and M34 produce limonene and D85 mainly produces b-pinene Peak identities were confirmed using standards, whose mass spectra and retention ... rich in serines and threonines and low in acidic and basic amino acids and about 45–70 amino acids long Usually they show only little homology The predictions using PREDOTAR and TARGETP indicate...

Ngày tải lên: 08/03/2014, 23:20

... higher d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the ... bands at about 42, 43 and 44 kDa were detected, while no bands were visible in chloroplast extracts (Fig 3) One could assume that in mitochondria the unprocessed protein, the mature protein and ... Qiagen) and 110 lL medium IV (4 g PEG 4000, mL H2O, 2.5 mL 0.8 m mannitol, mL m CaCl2) and incubated for 30 at 23 °C To remove the PEG mL medium II was added, centrifuged for at 95 g and °C, and...

Ngày tải lên: 16/03/2014, 18:20

... Cys22 and Cys82 were modified by alkylation, or replaced with alanine and valine, respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and ... library was then designed with randomized CDR3 loops, and displayed successfully on the surface of fd bacteriophages and panned for specific binding molecules [10] Third, and most recently we isolated ... CDR1 and CDR3 loops (Type2 VNAR) [5,11] Camelid VHHs typically possess disulphide linkages either between the CDR1 and )3, or CDR2 and )3 loops [13,14] Despite these similarities, the camel and...

Ngày tải lên: 17/03/2014, 10:20

... re-testing and validating the effects on cell growth by transforming W303.1C and plating the cells at 23 °C, 30 °C and 37 °C A cold-sensitive growth phenotype (where toxin is active and interferes ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... screens and for Temperature-dependent ricin A chain mutants their behaviour to be fully characterized in mammalian and plant systems Experimental procedures Yeast strain, manipulations and growth...

Ngày tải lên: 23/03/2014, 07:20

... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS chloroplasts were used as ... blue-native (BN) ⁄ PAGE and (2) colorlessnative (CN) ⁄ PAGE and BN ⁄ PAGE, we separated the Ndh complex from both MS and BS chloroplasts and determined its monomeric and dimeric state We also...

Ngày tải lên: 23/03/2014, 13:20

... Protein Assay solution (Bio-Rad, Sundbyberg, Sweden) Microbial strains Bacillus megaterium strain (Bm11), E coli strain (D21), and C albicans strain (ATCC 14043) were used to analyse the antimicrobial ... Institute of Iceland and 50 specimens were caught randomly After killing the fish by a concussion of the brain by striking of the cranium, mucus samples were collected by scraping the skin and then were ... (fraction 35 in Fig 2B), and purified to apparent homogeneity at 52% acetonitrile in the last step (fractions 58 and 59 in Fig 2C) SDS ⁄ PAGE of fractions 58 and 59 revealed a protein band with a mobility...

Ngày tải lên: 23/03/2014, 15:21

... collected and freezedried Standard procedures were employed analysing protein samples by SDS/PAGE using 18% polyacrylamide gels, and for the staining of proteins using Coomassie Brilliant Blue R-250 and ... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of ... following standard procedures, and a second round of PCR was performed using the same primers Obtained PCR products were ligated into pCR 2.1-TOPO cloning vectors and sequenced on both strands MUC15...

Ngày tải lên: 24/03/2014, 04:21

... works Genetics and the Origin of Species and Systematics and the Origin of Species, perhaps to emphasizethat they were using genetics and systematics primarily to advance understandingof the speciation ... marine fish and invertebrates suggest that isolation by distance occurs, but only over the largest geographic scales Isolated islands in the Pacific Ocean, like the Hawaiian Society and Islands, appear ... and two large circulation centers in the northern and southern hemispheresin both the Pacific and Atlantic Oceans Schopf (124) suggested that these basic patterns also occurred in the past, and...

Ngày tải lên: 28/03/2014, 16:20