song induced gene expression in the canary brain

... receptor binding parameters were determined using Scatchard analysis [32] The specific binding was deter- Page of 11 mined by subtracting non-specific binding from the total The binding parameters, ... activity in diabetic mice, but not in non diabetic mice [46] In our study, curcumin and insulin increased the dopamine D1 receptor expression levels in the cerebellum, which suggests that the curcumin ... hrs after the administration of morning dose of insulin and curcumin The results were expressed in terms of milligram per decilitre of blood Total Dopamine receptor binding studies in the cerebellum...

Ngày tải lên: 10/08/2014, 05:21

... through the Mutant Mouse Research Resource Centers [8,9] In addition, for some of the most interesting expression patterns, the project is developing lines where the BAC containing the gene promoter ... sites, resulting in a recombinant strain of mouse that eliminates a gene s expression only in the cells that express the Cre These lines will be a valuable resource for investigators looking to understand ... atlas, rather than choose sides in the ongoing debate about nomenclature in individual brain areas GenePaint points the user to several standard published adult and developmental brain atlases...

Ngày tải lên: 14/08/2014, 07:21

... This is the first report of differences in the levels of ovarian gene expression in an obese mouse model The most important finding of this study is that modified gene expression in the ovaries ... (in bold type) Genesto black mice involved in cholesterol biosynthesis exhibiting elevated expression (p < 0.05) in 180-day LY mice as comGenes (in bold type) involved in cholesterol biosynthesis ... into place, resulting in the inappropriate constitutive expression of the agouti gene [6] In the hypothalamus, the over-expressed agouti protein acts as an antagonist of melanocortin-4 receptors...

Ngày tải lên: 20/06/2014, 07:20

... current therapies In this study we provide the first gene array profiling of an estrogen responsive breast cancer cell line demonstrating that the combination of iodine and iodide alters gene expression ... 2-methoxyestradiol, decreasing 3, 4-estradiol quinone or indirectly via the inactivation of E2 The importance of the CYP1A1/CYP1B1 ratio in- vivo is evident in the increased presence of 4-OH-E2 in breast cancer ... required to illuminate the importance of these changes in response to iodine In addition to affecting estrogen metabolism, iodine/iodide may also inhibit estrogen induced transcription via increased...

Ngày tải lên: 08/08/2014, 17:20

... Article R135 Von Stetina et al R135.27 then used to query the Allen Brain Atlas [118] for expression in the mouse brain A gene was scored as 'expressed in the brain' if it had an intensity value of ... enriched in either embryonic or larval neurons In the future, it will be interesting to determine if these genes define stage-specific features of the developing nervous system The mRNA-tagging method ... (kinesin light chain) to regulate vesicular transport in neurons [68] Other members of this interacting complex, MKK-4 (MAP kinase kinase) and JNK-1 (Jun kinase) are also required for maintaining...

Ngày tải lên: 14/08/2014, 07:22

... coinciding with gene expression data By day these four cytokines were elevated, and there was also an increase in TNF-α and IL-18 in serum The ELISA data closely parallel the microarray mRNA expression ... genes were induced (Figure 4) Genes for the Bcl antagonists BCL2-antagonist of cell death, BH3 interacting domain death agonist, BCL-X, and BAK appeared to be induced in the later stages of infection; ... subtracting the average of the pre-infection baseline expression level from that animal, so that the values of each time point represent changes relative to the uninfected samples The genes whose expression...

Ngày tải lên: 14/08/2014, 08:20

... value of the training samples in the case of continuous variables The aggregate forest's output is then determined by averaging the regression values of the trees or using a weighted voting process ... accuracy, and in the regression context model error is the mean squared error The greater the decrease in normalized performance, the more instrumental was the variable (gene) in achieving the forest's ... basis, the random forest determines the importance score of each gene (variable) in the multivariate model The distribution of importance scores of the genes in the category is then compared with the...

Ngày tải lên: 14/08/2014, 08:20

... SLA2/Huntingtin-interacting protein Hip1 Y66H1B.3 462 784 Actin-binding cytoskeleton protein, filamin Y66H1B.2 119 902 Actin-binding cytoskeleton protein, filamin 766 20 Actin-binding protein Coronin, contains WD40 ... Actin regulatory proteins (gelsolin/villin family) Y71G12B.11 122 Talin W03F11.6 afd-1 - 393 Actin filament-binding protein Afadin F08A8.6 tag-138 - 474 Actin-binding protein SLA2/Huntingtin-interacting ... time course of gene expression (GeneSpring software; Agilent) for three independent samples at each time point for the HLH-1 induced dataset Line coloring in these graphs reflects the 6-hour value...

Ngày tải lên: 14/08/2014, 08:20

... boiling in a microwave and then immediately poured into a glass Coplin jar The Coplin jar was then placed into the 100°C water bath and the slides were then placed in to the Coplin jar for 20 minutes ... per point was 0.01 mm² and was determined at 4X magnification When measuring brain volume, the area inside the ventricles was included in the calculation (Figure 2.6.1 A) A random sampling of ... study examined gene expression in the brain of P0 Ts1Cje mice and found approximately a 1.5 fold expression level of trisomic genes (AMANO 2004) In the Ts65Dn mouse model, a study of the cerebellum...

Ngày tải lên: 24/08/2014, 12:33

... Abbreviations ICLAD Intracranial Large Artery Disease IDE Insulin-degrading enzyme IgG Immunoglobulin G IL12 Interleukin 12 IL-1β Interleukin-1β IL23 Interleukin 23 INSIG Insulin-induce gene IPA Ingenuity ... Cholesterol in the Brain The brain is the most cholesterol-rich organ in the body (Björkhem and Meaney, 2004) Although the human brain only accounts for 2% of the total body weight, it contains about ... Schipper, 2007) In the brain, cholesterol is divided into two pools: major pool with 70% of the brain cholesterol residing in myelin sheaths, and minor pool with the 30% residing in plasma membranes...

Ngày tải lên: 09/09/2015, 08:15

... [8,10-12] in cell culture studies Parthenolide appears to inhibit NF-κB by targeting the IκB (inhibitor of NF-κB) kinase complex [9] which might inhibit proinflammatory cytokine and chemokine gene expression ... determine whether differences in expression levels of each gene existed between the spleen and liver These organs contain macrophages and other cell types capable of responding to LPS and other inflammatory ... may account, at least in part, for the increased cytokine and COX-2 gene expression observed in the spleen when compared to the liver Figure mRNA expression levels in parthenolide and LPS co-treatment...

Ngày tải lên: 11/08/2014, 08:21

... saline, silica in physiological saline, or silica with Lv-shCD36 or Lv-shCD36-NC in physiological saline, was instilled into the lungs using a 14-gauge needle inserted into the trachea through the ... determine the silencing effect of CD36 in AMs in the silica+Lv-shCD36 group The results demonstrate that expression of CD36 mRNA in the silica+Lv-shCD36 group was significantly lower than in the ... silicosis was induced by an intratracheal administration of silica dust, resulting in varying degrees of fibrotic silicosis [15] Following silica -induced lung injury, AMs were stimulated and they secreted...

Ngày tải lên: 12/08/2014, 14:20

... than other endothelial cells in the body In keeping with the theory of endothelial cell activation having a role in ANFH, coagulation-related gene expression in particular serine (or cysteine) ... role in the development of early steroid -induced ANFH Determination of whether A2M over -expression in our study is either the result or the cause of the apoptosis found in our rats developing ... immunohistochemical findings for the two other genes of interest (COL2A1 and MIA) failed to show enhanced protein expression Discussion The early events in the pathogenesis of ANFH are incompletely understood...

Ngày tải lên: 12/08/2014, 14:22

... expression Genes detected and in following investigated for validation A) an induced expression of the PKN1 gene B) a repressed expression of the ZCD1 gene C) a repressed expression of BSG gene It ... receptor protein linked to signal transduction (CD147) (GO:0007166) The basigin protein was shown to be present in vascular endothelium in both in the non-neoplastic region of the brain and in tumor ... impact on the gene expression patterns, when analyzing the efficacy and the genetic consequences of targeted gene therapy in vitro (and in vivo) Microarray Study Differential Gene Expression...

Ngày tải lên: 03/11/2012, 11:44

... photosynthetic genes were induced by glucose was probably a result of the higher protein-synthetic activity Enzymes involved in glycolysis and the OPPP other than FbaA were not included in Table ... with respect to the accumulation of inhibitory metabolites [27,28] and gene expression [29] The expression of 10% of the genes was different in the linear growth phase compared to in the logarithmic ... evidence of the functional roles of the gene products has been obtained [8,13] The genes and ⁄ or enzymes involved in glycolysis and the OPPP, as studied to date in some detail in cyanobacteria, include...

Ngày tải lên: 16/03/2014, 04:20

... PTTHproducing cells (PTPCs) These results provide an insight into ecdysone receptor function in the brain during larval-pupal-adult development Results Expression of EcR-A and EcR-B1 in the Bombyx brain ... 3A–C, K L right), confirming that both isoforms are colocalized in PTPCs in the brain In the pupal brain, EcR-A and EcR-B1 expression was observed in TCCs and weakly in OLCs in addition to PTPCs ... arrows in (D) and (E) indicate the EcR-A- and EcR-B1-producing cells in the tritocerebral region of the pupal brain, respectively Red arrows indicate the EcR-Aor EcR-B1-producing cells in the subesophagal...

Ngày tải lên: 16/03/2014, 13:20

... tetracycline-resistance cassette (derived from the MiniTn5 [28]) was inserted and the resulting interrupted genes were cloned into the suicide vector, pKAS46 These constructs were then introduced into ... NifL-protein are located in the N-terminal domain [31] that binds the FAD-cofactor [17], one can speculate that the N-terminal domain of NifL might enter the lipid bilayer and contact the quinones ... transformed into RAS46 and recombinant strains carrying the chromosomally inserted plasmid (by means of single homologous recombination) were identified by their resistance to tetracycline and their inability...

Ngày tải lên: 17/03/2014, 10:20

... of the cytokine/chemokine levels in the F344 rat brain (Table 2) However, in the HIV-1Tg rat brain, the levels of the pro-inflammatory cytokines, IL-1β and IFN-γ, were significantly higher in the ... difference in the basal levels (SS+S group levels) of the cytokines/chemokines measured in the serum in the two strains of animals (Table 1) In the F344 serum, the levels of the pro-inflammatory cytokine, ... the anti-inflammatory cytokine, IL10, was elevated in the brain of both the SS+L (>3 fold) and LL+L (>2 fold) groups in the F344 rats, 12 ` but only in the brain of the SS+L (>2 fold) group in...

Ngày tải lên: 19/06/2014, 22:20

... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... level of expression for each gene (± standard error) compared to the house keeping gene GAPDH The increase in ADAMTS gene expression in the CrMFC approached significance and was included in the table ... compared to the lateral FC after ACL-X These data indicate that in this model the medial FC is more affected by the insults to the joint induced by transection of the ACL, which is in agreement...

Ngày tải lên: 20/06/2014, 00:20

... CA) The repression activity of the Mad-SID is mediated through interactions of the Mad N-terminus with the mammalian Sin3 co-repressor protein [10,11] The effectiveness of the Sin3 binding domain ... Mad in silencing gene expression when fused to DNA binding proteins has been demonstrated in two studies One group fused the Mad SID to the c-Jun DNA binding domain and demonstrated specific inhibition ... is involved in modulating the signaling cascade activated by PRL in granulosa cells [48] The Ctla4, Eda, and Mrgpre genes are in the signaling category but are not found in the OKDB However, they...

Ngày tải lên: 20/06/2014, 07:20